SE0202552D0 - Recovery of plasmids in an aqueous two-phase system - Google Patents

Recovery of plasmids in an aqueous two-phase system

Info

Publication number
SE0202552D0
SE0202552D0 SE0202552A SE0202552A SE0202552D0 SE 0202552 D0 SE0202552 D0 SE 0202552D0 SE 0202552 A SE0202552 A SE 0202552A SE 0202552 A SE0202552 A SE 0202552A SE 0202552 D0 SE0202552 D0 SE 0202552D0
Authority
SE
Sweden
Prior art keywords
polymer
plasmid dna
aqueous
plasmids
recovery
Prior art date
Application number
SE0202552A
Other languages
English (en)
Inventor
Cecilia Kepka
Folke Tjerneld
Jenny Rhodin
Original Assignee
Amersham Biosciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Ab filed Critical Amersham Biosciences Ab
Priority to SE0202552A priority Critical patent/SE0202552D0/sv
Publication of SE0202552D0 publication Critical patent/SE0202552D0/sv
Priority to AT03736399T priority patent/ATE362529T1/de
Priority to US10/526,089 priority patent/US7553658B2/en
Priority to DE60313870T priority patent/DE60313870D1/de
Priority to PCT/SE2003/001016 priority patent/WO2004020629A1/en
Priority to EP03736399A priority patent/EP1532243B1/en
Priority to JP2004532487A priority patent/JP4699757B2/ja
Priority to AU2003237740A priority patent/AU2003237740A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SE0202552A 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system SE0202552D0 (sv)

Priority Applications (8)

Application Number Priority Date Filing Date Title
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system
AT03736399T ATE362529T1 (de) 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem
US10/526,089 US7553658B2 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system
DE60313870T DE60313870D1 (de) 2002-08-27 2003-06-17 Gewinnung von plasmiden in einem wässrigen zweiphasensystem
PCT/SE2003/001016 WO2004020629A1 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system
EP03736399A EP1532243B1 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system
JP2004532487A JP4699757B2 (ja) 2002-08-27 2003-06-17 水性二相系でのプラスミドの回収
AU2003237740A AU2003237740A1 (en) 2002-08-27 2003-06-17 Recovery of plasmids in an aqueous two-phase system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system

Publications (1)

Publication Number Publication Date
SE0202552D0 true SE0202552D0 (sv) 2002-08-27

Family

ID=20288830

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0202552A SE0202552D0 (sv) 2002-08-27 2002-08-27 Recovery of plasmids in an aqueous two-phase system

Country Status (8)

Country Link
US (1) US7553658B2 (sv)
EP (1) EP1532243B1 (sv)
JP (1) JP4699757B2 (sv)
AT (1) ATE362529T1 (sv)
AU (1) AU2003237740A1 (sv)
DE (1) DE60313870D1 (sv)
SE (1) SE0202552D0 (sv)
WO (1) WO2004020629A1 (sv)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008529516A (ja) * 2005-02-11 2008-08-07 インヴィトロジェン ダイナル エーエス エチレングリコール多量体の使用を含む核酸の単離方法
EP1762616A1 (de) * 2005-09-13 2007-03-14 Qiagen GmbH Verfahren zur selektiven Anreicherung von doppelsträngiger DNA aus Nukleinsäuregemischen
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8268915B2 (en) 2007-06-19 2012-09-18 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
US8999702B2 (en) 2008-06-11 2015-04-07 Emd Millipore Corporation Stirred tank bioreactor
JP2012511929A (ja) 2008-12-16 2012-05-31 イー・エム・デイー・ミリポア・コーポレイシヨン 攪拌タンク反応器及び方法
EP2571903B1 (en) 2010-05-17 2019-09-04 EMD Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
PT105438B (pt) * 2010-12-14 2014-08-14 Univ Do Minho Isolamento de dna plasmídico em sistemas de duas fases aquosas utilizando polímeros neutros derivatizados com grupos catiónicos
US9169379B2 (en) 2013-07-15 2015-10-27 King Fahd University Of Petroleum And Minerals Aqueous two-phase systems
CN114839374A (zh) 2017-03-28 2022-08-02 相达生物科技国际有限公司 用于在液体活检中准确诊断疾病靶向生物标记的方法
WO2018222972A1 (en) 2017-06-01 2018-12-06 Yin To Chiu Phase separation behavior modifying agents for aqueous two-phase separation within porous material
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
WO2019143943A2 (en) * 2018-01-19 2019-07-25 Yin To Chiu Composition and method for concentration and enrichment of nucleic acids
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
US11479765B2 (en) 2018-01-19 2022-10-25 Phase Scientific International, Ltd. Method of isolating exosomes using encapsulation and aqueous micellar system
JP7370987B2 (ja) 2018-01-19 2023-10-30 フェーズ サイエンティフィック インターナショナル リミテッド 固液相系を用いて核酸を単離して精製するための方法
CN112375109B (zh) * 2020-11-20 2023-03-28 华东理工大学 利用烷基酚聚氧乙烯聚氧丙烯醚npe-108分离纯化螺旋霉素
WO2023174022A1 (en) * 2022-03-16 2023-09-21 The University Of Hong Kong Synergistic control and dynamic assembly of viscoelastic networks and biomolecular condensates by aqueous liquid-liquid phase separation and liquid-solid phase separation (aqll-ls ps2)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9603304D0 (sv) * 1996-09-11 1996-09-11 Pharmacia & Upjohn Ab Process for purifying a compound
CA2277199A1 (en) * 1996-09-11 1998-03-19 Hans Ageland A process for purifying apolipoproteins and a composition for use in the process

Also Published As

Publication number Publication date
JP2005537004A (ja) 2005-12-08
JP4699757B2 (ja) 2011-06-15
US7553658B2 (en) 2009-06-30
EP1532243B1 (en) 2007-05-16
AU2003237740A1 (en) 2004-03-19
US20060166349A1 (en) 2006-07-27
ATE362529T1 (de) 2007-06-15
WO2004020629A1 (en) 2004-03-11
EP1532243A1 (en) 2005-05-25
DE60313870D1 (de) 2007-06-28

Similar Documents

Publication Publication Date Title
SE0202552D0 (sv) Recovery of plasmids in an aqueous two-phase system
DK1163260T3 (da) Fremgangsmåde til adskillelse af proteiner
WO2003064605A3 (en) Crude biological derivatives competent for nucleic acid detection
AR031912A2 (es) Subtilasa modificada, acido nucleico, vector, celula microbiana y metodo para producirla
EA200702156A1 (ru) Применение белков в качестве деэмульгаторов
EP1631314A4 (en) METHOD FOR MODIFYING BINDING SPECIFICITY OF PLASMIC PROTEINS USING OXIDATION-REDUCTION REACTIONS
WO2005061716A3 (de) Expressionsvektor zur antibiotikafreien expression
ATE414674T1 (de) Kreisel und verfahren zur dessen herstellung
WO2001059094A3 (en) Method of regulating transcription in a cell by altering remodeling of cromatin
BR112012016449A2 (pt) adesivo de laminação monocomponente tendo reticulação de silano
ATE378426T1 (de) Neue sequenz um die expression einer nukleinsäure zu erhöhen
WO2002010393A3 (en) Isolated nucleic acid molecules which encode t cell inducible factors, or interleukin-21, the proteins encoded, and uses thereof
PL1960521T3 (pl) Sposób i zestaw do rozdzielania, oczyszczania i odzyskiwania długo- i krótkołańcuchowych kwasów nukleinowych
WO2004033718A3 (en) Trap-tagging: a novel method for the identification and purification of rna-protein complexes
BR9810650A (pt) Vetor para expressão de proteìna heteróloga e métodos para extrair proteìna recombinante e para purificar insulina recombinante isolada
WO2009005547A3 (en) Method for electrostatic trapping of molecules in nanopores
DK1567474T3 (da) Fremgangsmåde til oprensning af diacerein
ATE513043T1 (de) Gegen denaturierungsmittel stabile und/oder proteaseresistente, chaperonähnliche oligomere proteine, diese kodierende polynukleotide sowie ihre verwendungen
WO2004011637A3 (en) Modified adamts4 molecules and method of use thereof
TW200506067A (en) Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence
WO2005076766A3 (en) Transient production of pharmaceutically important proteins in plants
WO2008025558A3 (en) Affinity marker comprising a first and a second tag and its use
PT1322753E (pt) Novas redutases do acido 2,5-diceto-l-gluconico e metodos de utilizacao
WO2001094573A3 (en) Processing of plasmid-containing fluids
ATE464380T1 (de) Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen