SE0004777D0 - Separation of X and Y sperm cells - Google Patents

Separation of X and Y sperm cells

Info

Publication number
SE0004777D0
SE0004777D0 SE0004777A SE0004777A SE0004777D0 SE 0004777 D0 SE0004777 D0 SE 0004777D0 SE 0004777 A SE0004777 A SE 0004777A SE 0004777 A SE0004777 A SE 0004777A SE 0004777 D0 SE0004777 D0 SE 0004777D0
Authority
SE
Sweden
Prior art keywords
sperm cells
separation
medium
density
centrifugation
Prior art date
Application number
SE0004777A
Other languages
Swedish (sv)
Inventor
Haakan Pertoft
Boerje Norling
Original Assignee
Amersham Pharm Biotech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharm Biotech Ab filed Critical Amersham Pharm Biotech Ab
Priority to SE0004777A priority Critical patent/SE0004777D0/en
Publication of SE0004777D0 publication Critical patent/SE0004777D0/en
Priority to EP01997697A priority patent/EP1336088B1/en
Priority to DE60112604T priority patent/DE60112604T2/en
Priority to PCT/EP2001/013551 priority patent/WO2002042767A2/en
Priority to AU2002223035A priority patent/AU2002223035A1/en
Priority to CA2428041A priority patent/CA2428041C/en
Priority to AT01997697T priority patent/ATE301830T1/en
Priority to JP2002544656A priority patent/JP4684533B2/en
Priority to US10/416,440 priority patent/US20040038356A1/en
Priority to ES01997697T priority patent/ES2249494T3/en
Priority to JP2002553093A priority patent/JP2004516035A/en
Priority to EP01985895A priority patent/EP1344042A2/en
Priority to AU2002235787A priority patent/AU2002235787A1/en
Priority to PCT/EP2001/014934 priority patent/WO2002052244A2/en
Priority to CA002429481A priority patent/CA2429481A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/05—Investigating sedimentation of particle suspensions in blood

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)

Abstract

The present invention relates to separation of X- and Y-sperm cells from each other by centrifugation in a colloidal density medium. In the method X and Y sperm cells can be separated from each other in a medium formula which has a density close to that of X and Y sperm cells of the species in question. The method is simple and rapid and provides highly purified X and Y sperm cells, respectively in high yield.
SE0004777A 2000-11-22 2000-12-22 Separation of X and Y sperm cells SE0004777D0 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
SE0004777A SE0004777D0 (en) 2000-12-22 2000-12-22 Separation of X and Y sperm cells
ES01997697T ES2249494T3 (en) 2000-11-22 2001-11-21 GRADUAL METHOD INTENDED TO RECOVER OR ELIMINATE BIOLOGICAL SUBSTANCES BY FLOATING FROM A LOW DENSITY COLLOIDAL ENVIRONMENT.
AT01997697T ATE301830T1 (en) 2000-11-22 2001-11-21 STEP-BY-STEP PROCESS FOR RECOVERING OR ELIMINATION OF BIOLOGICAL SUBSTANCES BY FLOTATION FROM AN UNDERLYING COLLOIDAL MEDIUM
US10/416,440 US20040038356A1 (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium
PCT/EP2001/013551 WO2002042767A2 (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium
AU2002223035A AU2002223035A1 (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium
CA2428041A CA2428041C (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium
EP01997697A EP1336088B1 (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium
JP2002544656A JP4684533B2 (en) 2000-11-22 2001-11-21 A multi-step method for recovering or removing biological samples by floating from an underlying colloidal medium
DE60112604T DE60112604T2 (en) 2000-11-22 2001-11-21 STEP-BY-STEP PROCESS FOR RECOVERING OR REMOVING BIOLOGICAL SUBSTANCES BY FLOTATION FROM A COLLIDAL MEDIUM THROUGHOUT
CA002429481A CA2429481A1 (en) 2000-12-22 2001-12-18 Separation of x- and y-sperm cells
JP2002553093A JP2004516035A (en) 2000-12-22 2001-12-18 Separation of X and sperm cells
EP01985895A EP1344042A2 (en) 2000-12-22 2001-12-18 Separation of x- and y-sperm cells
AU2002235787A AU2002235787A1 (en) 2000-12-22 2001-12-18 Separation of x- and y-sperm cells
PCT/EP2001/014934 WO2002052244A2 (en) 2000-12-22 2001-12-18 Separation of x- and y-sperm cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0004777A SE0004777D0 (en) 2000-12-22 2000-12-22 Separation of X and Y sperm cells

Publications (1)

Publication Number Publication Date
SE0004777D0 true SE0004777D0 (en) 2000-12-22

Family

ID=20282358

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0004777A SE0004777D0 (en) 2000-11-22 2000-12-22 Separation of X and Y sperm cells

Country Status (6)

Country Link
EP (1) EP1344042A2 (en)
JP (1) JP2004516035A (en)
AU (1) AU2002235787A1 (en)
CA (1) CA2429481A1 (en)
SE (1) SE0004777D0 (en)
WO (1) WO2002052244A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4323571B2 (en) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド Optical device
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
CA2822983C (en) 2000-11-29 2017-05-09 Xy, Llc System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
BRPI0313163B1 (en) 2002-08-01 2015-11-17 Univ Colorado State low pressure sperm cell separation system
AU2003265471B2 (en) 2002-08-15 2009-08-06 Xy, Llc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
BR0300604A (en) * 2003-02-17 2004-11-03 Fundacao De Amparo A Pesquisa Sex selection process of mammalian sperm and quality control method of frozen sexed semen doses
DK2305173T3 (en) 2003-03-28 2016-08-22 Inguran Llc Apparatus and method for providing sorted particles
US20060263829A1 (en) 2003-05-15 2006-11-23 Evans Kenneth M Efficient haploid cell sorting flow cytometer systems
AU2005229073B2 (en) 2004-03-29 2010-08-19 Inguran, Llc Sperm suspensions for use in insemination
US7833147B2 (en) 2004-07-22 2010-11-16 Inguran, LLC. Process for enriching a population of sperm cells
DE102013218528B3 (en) 2013-09-16 2014-12-24 ViaMed GmbH Device for the enrichment of spermatozoa
WO2019004217A1 (en) * 2017-06-30 2019-01-03 国立大学法人広島大学 Method for separating mammalian sperms, artificial insemination method, and in vitro fertilization method
JP7142842B2 (en) * 2017-06-30 2022-09-28 国立大学法人広島大学 Mammalian sperm separation method, artificial insemination method and in vitro fertilization method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4327177A (en) * 1969-04-10 1982-04-27 Wallace Shrimpton Method and means for controlling the sex of mammalian offspring and product therefor
US4927749A (en) * 1986-04-09 1990-05-22 Jeanette Simpson Reagent for cell separation
US4759344A (en) * 1986-08-11 1988-07-26 Wang Fu Nan Wang's tubules for sperm preparations used for IVF-ET-GIFT and artificial inseminations
US5437878A (en) * 1993-11-10 1995-08-01 Nabisco, Inc. Chewing gum exhibiting reduced adherence to dental work
EP1025904B1 (en) * 1999-02-02 2007-05-09 Nipro Corporation Tube for sperm washing and concentration and method for sperm washing and concentration

Also Published As

Publication number Publication date
JP2004516035A (en) 2004-06-03
EP1344042A2 (en) 2003-09-17
WO2002052244A3 (en) 2002-12-05
AU2002235787A1 (en) 2002-07-08
WO2002052244A2 (en) 2002-07-04
CA2429481A1 (en) 2002-07-04

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