AU2002223035A1 - Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium - Google Patents

Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium

Info

Publication number
AU2002223035A1
AU2002223035A1 AU2002223035A AU2303502A AU2002223035A1 AU 2002223035 A1 AU2002223035 A1 AU 2002223035A1 AU 2002223035 A AU2002223035 A AU 2002223035A AU 2303502 A AU2303502 A AU 2303502A AU 2002223035 A1 AU2002223035 A1 AU 2002223035A1
Authority
AU
Australia
Prior art keywords
underlayered
flotation
recover
biological substances
colloidal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002223035A
Inventor
Borje Norling
Hakan Pertoft
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0004271A external-priority patent/SE0004271D0/en
Priority claimed from SE0004777A external-priority patent/SE0004777D0/en
Application filed by Amersham Bioscience AB filed Critical Amersham Bioscience AB
Publication of AU2002223035A1 publication Critical patent/AU2002223035A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/05—Investigating sedimentation of particle suspensions in blood

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Paper (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)

Abstract

The present invention relates to a method and kit for discontinuous density gradient centrifugation to separate a minimum of two substances in a sample. In the method and kit use is made of colloidal density media and low density solutions, such as buffer solutions. The sample is mixed with colloidal media and placed at the bottom of a centrifuge tube and desired substances flotate to the lower density media following centrifugation.
AU2002223035A 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium Abandoned AU2002223035A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE0004271A SE0004271D0 (en) 2000-11-22 2000-11-22 Method and kit for discontinuous density gradient centrifugation
SE0004271-3 2000-11-22
SE0004777A SE0004777D0 (en) 2000-12-22 2000-12-22 Separation of X and Y sperm cells
SE0004777-9 2000-12-22
PCT/EP2001/013551 WO2002042767A2 (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium

Publications (1)

Publication Number Publication Date
AU2002223035A1 true AU2002223035A1 (en) 2002-06-03

Family

ID=26655316

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002223035A Abandoned AU2002223035A1 (en) 2000-11-22 2001-11-21 Step-wise process to recover or eliminate biological substances by flotation from underlayered colloidal medium

Country Status (9)

Country Link
US (1) US20040038356A1 (en)
EP (1) EP1336088B1 (en)
JP (1) JP4684533B2 (en)
AT (1) ATE301830T1 (en)
AU (1) AU2002223035A1 (en)
CA (1) CA2428041C (en)
DE (1) DE60112604T2 (en)
ES (1) ES2249494T3 (en)
WO (1) WO2002042767A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070238088A1 (en) * 2006-03-29 2007-10-11 General Electric Company Hydrophilic functionalized colloidal silica compositions, methods of making, and uses therefor
EP2552934B1 (en) * 2010-03-30 2015-07-15 Novozymes A/S Crystal metabolite recovery
US9217697B2 (en) 2012-11-30 2015-12-22 Rarecyte, Inc. Apparatus, system, and method for collecting a target material
US9513291B2 (en) 2012-11-30 2016-12-06 Rarecyte, Inc. Apparatus, system, and method for collecting a target material
CN108624552B (en) * 2018-03-27 2020-11-27 中国农业科学院北京畜牧兽医研究所 Method for obtaining high-purity chicken sperms

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4927750A (en) * 1986-04-09 1990-05-22 Jeanette Simpson Cell separation process
US4927749A (en) * 1986-04-09 1990-05-22 Jeanette Simpson Reagent for cell separation
US4957963A (en) * 1988-08-05 1990-09-18 Dow Corning Corporation Silicone water based elastomers
CA2066761C (en) * 1989-09-20 1999-11-16 Patrick Soon-Shiong Cell separation invention
US5275933A (en) * 1992-09-25 1994-01-04 The Board Of Trustees Of The Leland Stanford Junior University Triple gradient process for recovering nucleated fetal cells from maternal blood
AU2438497A (en) * 1996-04-05 1997-10-29 Johns Hopkins University, The A method of enriching rare cells

Also Published As

Publication number Publication date
US20040038356A1 (en) 2004-02-26
CA2428041C (en) 2012-08-07
DE60112604T2 (en) 2006-06-08
JP4684533B2 (en) 2011-05-18
WO2002042767A3 (en) 2002-09-26
CA2428041A1 (en) 2002-05-30
EP1336088A2 (en) 2003-08-20
DE60112604D1 (en) 2005-09-15
WO2002042767A2 (en) 2002-05-30
EP1336088B1 (en) 2005-08-10
ES2249494T3 (en) 2006-04-01
JP2004514548A (en) 2004-05-20
ATE301830T1 (en) 2005-08-15

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