RU99111046A - METHOD FOR DETERMINING FUNCTIONAL ACTIVITY OF HUMAN KOMPLEMENT COMPONENT C4 - Google Patents

METHOD FOR DETERMINING FUNCTIONAL ACTIVITY OF HUMAN KOMPLEMENT COMPONENT C4

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Publication number
RU99111046A
RU99111046A RU99111046/14A RU99111046A RU99111046A RU 99111046 A RU99111046 A RU 99111046A RU 99111046/14 A RU99111046/14 A RU 99111046/14A RU 99111046 A RU99111046 A RU 99111046A RU 99111046 A RU99111046 A RU 99111046A
Authority
RU
Russia
Prior art keywords
component
human
functional activity
komplement
determining functional
Prior art date
Application number
RU99111046/14A
Other languages
Russian (ru)
Other versions
RU2162601C1 (en
Inventor
Леонид Васильевич Козлов
Татьяна Геннадиевна Скороходова
Тамара Николаевна Баталова
Владимир Михайлович Лахтин
Наталья Сергеевна Матвеевская
Валентина Алексеевна Гузова
Всеволод Львович Дьяков
Original Assignee
Московский научно-исследовательский институт эпидемиологии и микробиологии им. Г.Н. Габричевского
Filing date
Publication date
Application filed by Московский научно-исследовательский институт эпидемиологии и микробиологии им. Г.Н. Габричевского filed Critical Московский научно-исследовательский институт эпидемиологии и микробиологии им. Г.Н. Габричевского
Priority to RU99111046/14A priority Critical patent/RU2162601C1/en
Priority claimed from RU99111046/14A external-priority patent/RU2162601C1/en
Application granted granted Critical
Publication of RU2162601C1 publication Critical patent/RU2162601C1/en
Publication of RU99111046A publication Critical patent/RU99111046A/en

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Claims (1)

Способ определения функциональной активности компонента С4 комплемента человека, отличающийся тем, что сорбируют в лунках микропанели липополисахарид из клеточной стенки грамотрицательных бактерий, в лунки микропанелей вносят раствор, содержащий компонент С4, и сыворотку морской свинки, проводят инкубацию, а затем вносят конъюгат фермента с антителами против компонента С4 человека и субстрат этого фермента, после чего проводят определение активности С4 по продукту ферментативной реакции.A method for determining the functional activity of a human complement component C4, characterized in that a lipopolysaccharide is sorbed from the cell wall of gram-negative bacteria in the micropanel wells, a solution containing the C4 component and guinea pig serum are added to the micropanel wells, incubated, and then an enzyme conjugate with antibodies against human C4 component and the substrate of this enzyme, after which the C4 activity is determined by the product of the enzymatic reaction.
RU99111046/14A 1999-05-26 1999-05-26 Method of assay of functional activity of component c4 of human complement RU2162601C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU99111046/14A RU2162601C1 (en) 1999-05-26 1999-05-26 Method of assay of functional activity of component c4 of human complement

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU99111046/14A RU2162601C1 (en) 1999-05-26 1999-05-26 Method of assay of functional activity of component c4 of human complement

Publications (2)

Publication Number Publication Date
RU2162601C1 RU2162601C1 (en) 2001-01-27
RU99111046A true RU99111046A (en) 2001-04-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU99111046/14A RU2162601C1 (en) 1999-05-26 1999-05-26 Method of assay of functional activity of component c4 of human complement

Country Status (1)

Country Link
RU (1) RU2162601C1 (en)

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