IL93862A0 - Enzymatic assay kit and method applicable to whole cells - Google Patents

Enzymatic assay kit and method applicable to whole cells

Info

Publication number
IL93862A0
IL93862A0 IL93862A IL9386290A IL93862A0 IL 93862 A0 IL93862 A0 IL 93862A0 IL 93862 A IL93862 A IL 93862A IL 9386290 A IL9386290 A IL 9386290A IL 93862 A0 IL93862 A0 IL 93862A0
Authority
IL
Israel
Prior art keywords
determination
cell population
endogenous
assay kit
whole cells
Prior art date
Application number
IL93862A
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of IL93862A0 publication Critical patent/IL93862A0/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a kit for determination of the endogenous cell enzymes characteristic of a cell population or subpopulation, comprising, as components: a) a solid support to which one or more monoclonal antibodies directed towards surface antigens of the cell population to be determined are bound by covalent bonding or by physical adsorption; b) one or more solutions supplying the necessary reagents (substrate and chromogen) for visualising the activity of the endogenous enzyme to be determined. The invention also relates to a method for enzymatic determination of the endogenous enzymes of a cell population or subpopulation. The kit for determination and the enzymatic method according to the invention may be used for the determination of myeloperoxidase in the granulocytes in the blood or urine.
IL93862A 1989-03-24 1990-03-23 Enzymatic assay kit and method applicable to whole cells IL93862A0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8903943A FR2644894B1 (en) 1989-03-24 1989-03-24 ENZYMATIC DOSAGE KIT AND METHOD APPLICABLE TO WHOLE CELLS

Publications (1)

Publication Number Publication Date
IL93862A0 true IL93862A0 (en) 1990-12-23

Family

ID=9380065

Family Applications (1)

Application Number Title Priority Date Filing Date
IL93862A IL93862A0 (en) 1989-03-24 1990-03-23 Enzymatic assay kit and method applicable to whole cells

Country Status (11)

Country Link
EP (1) EP0389381B1 (en)
JP (1) JPH02298866A (en)
KR (1) KR900014887A (en)
AT (1) ATE113725T1 (en)
CA (1) CA2012934A1 (en)
DE (1) DE69013730T2 (en)
FI (1) FI96723C (en)
FR (1) FR2644894B1 (en)
IE (1) IE66587B1 (en)
IL (1) IL93862A0 (en)
PT (1) PT93548A (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5385822A (en) * 1988-05-02 1995-01-31 Zynaxis, Inc. Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population
US5256532A (en) * 1988-05-02 1993-10-26 Zynaxis Technologies, Inc. Methods, reagents and test kits for determination of subpopulations of biological entities
DE19952155A1 (en) * 1999-10-29 2001-05-03 Volkswagen Ag Cable, pipe or wire fastening clip for use in road vehicle has L-shaped bracket holding U-section clip member with curved arm defining space for cable
US7780950B2 (en) 2002-01-02 2010-08-24 The Cleveland Clinic Foundation Systemic marker for monitoring anti-inflammatory and antioxidant actions of therapeutic agents
ES2429528T3 (en) 2001-01-02 2013-11-15 The Cleveland Clinic Foundation Myeloperoxidase, a risk indicator for cardiovascular disease
US7459286B1 (en) 2003-10-22 2008-12-02 The Cleveland Clinic Foundation Assessing the risk of a major adverse cardiac event in patients with chest pain
LT2433138T (en) * 2009-05-18 2017-03-10 Technische Universität Graz Method for detecting a wound infection
CN104950111A (en) * 2015-05-22 2015-09-30 北京协和洛克生物技术有限责任公司 Liquid chip kit for quantitatively detecting concentration of myeloperoxidase (MPO) in sample and preparation method of liquid chip kit

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3047860A1 (en) * 1980-12-18 1982-07-15 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR DETERMINING HLA ANTIGENS
US4591570A (en) * 1983-02-02 1986-05-27 Centocor, Inc. Matrix of antibody-coated spots for determination of antigens
FR2571498B1 (en) * 1984-10-04 1988-04-08 Immunotech Sa METHOD FOR SEPARATING CELLS USING LOW DENSITY ANTIBODIES AND BALLS
DE3438683A1 (en) * 1984-10-22 1986-04-24 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR DETERMINING PEROXIDASE
US4770995A (en) * 1985-08-29 1988-09-13 New York Blood Center, Inc Detection of the sensitivity of cells to the effects of tumor necrosis factor and lymphotoxin
DE3541033A1 (en) * 1985-11-19 1987-05-21 Boehringer Mannheim Gmbh METHOD FOR QUANTIFYING CELL POPULATIONS OR SUBPOPULATIONS AND REAGENT SUITABLE FOR THIS
CA1326435C (en) * 1987-03-13 1994-01-25 Wallace H. Coulter Method and apparatus for rapid mixing of small volumes for enhancing biological reactions
US5100777A (en) * 1987-04-27 1992-03-31 Tanox Biosystems, Inc. Antibody matrix device and method for evaluating immune status

Also Published As

Publication number Publication date
PT93548A (en) 1990-11-07
IE66587B1 (en) 1996-01-24
FI96723B (en) 1996-04-30
DE69013730T2 (en) 1995-06-01
DE69013730D1 (en) 1994-12-08
IE901081L (en) 1990-09-24
FI901468A0 (en) 1990-03-23
KR900014887A (en) 1990-10-25
FR2644894B1 (en) 1994-05-13
JPH02298866A (en) 1990-12-11
CA2012934A1 (en) 1990-09-24
FR2644894A1 (en) 1990-09-28
FI96723C (en) 1996-08-12
EP0389381A1 (en) 1990-09-26
EP0389381B1 (en) 1994-11-02
ATE113725T1 (en) 1994-11-15

Similar Documents

Publication Publication Date Title
AU2294888A (en) Immunometric assay kit and method applicable to whole cells
EP0803577A3 (en) Method for detecting and/or determining ATP from microorganism cells in a sample
AU591636B2 (en) Assay method employing fluorescent cell incorporative dye
GR3034288T3 (en) Method of stabilizing enzyme conjugates.
AU641399B2 (en) Method for determining nucleic acid
IL93862A0 (en) Enzymatic assay kit and method applicable to whole cells
AU8135987A (en) Novel immunochemical method for assaying stable glycosylated hemoglobin
EP0825258A4 (en) Method for determining 1,5-anhydroglucitol
AU559670B2 (en) Enzymatic process for the determination of beta-lactam antibiotics
IE850266L (en) Chromohenic support immunoassay utilizing labelled¹complement components
AU6647490A (en) Enzyme assay and assay kit to measure cellular activation
AU2747084A (en) Fluorometric assay of chymopapain and reagents therefor
EP0173276A3 (en) Method for the determination of substrate or enzyme activity
AU5057785A (en) A method for determining a ligand
AU3046089A (en) Method for assaying chondrocalcine
IL59673A0 (en) Tabilization of working reagent solutions containing nadh,nadph,and/or enzymes,and the use of such stabilized reagents in enzyme or substrate assays

Legal Events

Date Code Title Description
KB Patent renewed
KB Patent renewed
HC Change of name of proprietor(s)
KB Patent renewed
KB Patent renewed