AU2294888A - Immunometric assay kit and method applicable to whole cells - Google Patents

Immunometric assay kit and method applicable to whole cells

Info

Publication number
AU2294888A
AU2294888A AU22948/88A AU2294888A AU2294888A AU 2294888 A AU2294888 A AU 2294888A AU 22948/88 A AU22948/88 A AU 22948/88A AU 2294888 A AU2294888 A AU 2294888A AU 2294888 A AU2294888 A AU 2294888A
Authority
AU
Australia
Prior art keywords
assay
subpopulation
surface antigens
cell population
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU22948/88A
Other versions
AU621310B2 (en
Inventor
Dominique Carriere
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Aventis France
Original Assignee
Sanofi Synthelabo SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Synthelabo SA filed Critical Sanofi Synthelabo SA
Publication of AU2294888A publication Critical patent/AU2294888A/en
Application granted granted Critical
Publication of AU621310B2 publication Critical patent/AU621310B2/en
Assigned to SANOFI-SYNTHELABO reassignment SANOFI-SYNTHELABO Request to Amend Deed and Register Assignors: SANOFI
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A kit for the assay of the surface antigens characteristic of a cell population or subpopulation comprising, as components: a) a solid support on which are fixed by covalent bonding or by physical adsorption one or more monoclonal antibodies directed against the surface antigens of the cellular population to be assayed; b) one or more solutions, each containing a monoclonal antibody specific for the cell population or subpopulation to be assayed, labelled with a radioisotope probe or an enzyme probe; c) in the case of antibodies marked with an enzyme probe, one or more solutions contributing the necessary reagents (substrate and chromogen) for detecting the enzyme activity. The invention also relates to a process for immunometric assay of the surface antigens of a cell population or subpopulation. The assay kit and the immunometric process according to the invention can be employed for the total assay of the population of T lymphocytes and/or of the subpopulations of T4 and T8 lymphocytes.
AU22948/88A 1987-09-30 1988-09-29 Immunometric assay kit and method applicable to whole cells Ceased AU621310B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8713537A FR2621128B1 (en) 1987-09-30 1987-09-30 IMMUNOMETRIC ASSAY KIT AND METHOD FOR WHOLE CELLS
FR8713537 1987-09-30

Publications (2)

Publication Number Publication Date
AU2294888A true AU2294888A (en) 1989-04-06
AU621310B2 AU621310B2 (en) 1992-03-12

Family

ID=9355391

Family Applications (1)

Application Number Title Priority Date Filing Date
AU22948/88A Ceased AU621310B2 (en) 1987-09-30 1988-09-29 Immunometric assay kit and method applicable to whole cells

Country Status (16)

Country Link
EP (1) EP0311492B1 (en)
JP (1) JPH01121755A (en)
KR (1) KR0126236B1 (en)
AT (1) ATE103711T1 (en)
AU (1) AU621310B2 (en)
CA (1) CA1340973C (en)
DE (1) DE3888779T2 (en)
DK (1) DK174032B1 (en)
ES (1) ES2053785T3 (en)
FI (1) FI95752C (en)
FR (1) FR2621128B1 (en)
HK (1) HK1001570A1 (en)
IE (1) IE63426B1 (en)
IL (1) IL87882A (en)
NO (1) NO177444C (en)
PT (1) PT88615B (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009894A1 (en) * 1990-11-29 1992-06-11 Zynaxis Technologies, Incorporated Methods for detection and quantitation of cell subsets within subpopulations of a mixed cell population
US5256532A (en) * 1988-05-02 1993-10-26 Zynaxis Technologies, Inc. Methods, reagents and test kits for determination of subpopulations of biological entities
AU642904B2 (en) * 1989-09-22 1993-11-04 La Mina Ltd Urine testing module and method of collecting urine antigen
WO1994022013A1 (en) * 1993-03-22 1994-09-29 Zynaxis, Inc. Immunoassay for determination of cells
US5466574A (en) * 1991-03-25 1995-11-14 Immunivest Corporation Apparatus and methods for magnetic separation featuring external magnetic means
US5500348A (en) * 1992-11-04 1996-03-19 Shionogi & Co., Ltd. Basophil-binding monoclonal antibody, method for separation of basophils, method for chemical mediator release from basophils, and method for testing release of basophil-derived chemical mediators
WO1996027132A1 (en) * 1995-02-28 1996-09-06 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
US5658745A (en) * 1995-02-17 1997-08-19 E. I. Du Pont De Nemours And Company Cell enumeration immunoassay
US5795470A (en) * 1991-03-25 1998-08-18 Immunivest Corporation Magnetic separation apparatus
US7005271B1 (en) 1994-07-23 2006-02-28 Jean-Marie Freyssinet Method for the determination of the prethrombotic state

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2684186B1 (en) * 1991-11-25 1994-02-25 Pasteur Sanofi Diagnostics KIT FOR THE RAPID ENUMERATION OF GRANULOCYTES, AND METHOD USING THE SAME.
ES2066722B1 (en) * 1993-05-20 1995-11-01 Univ Pais Vasco PROCEDURE FOR THE DIAGNOSIS OF INVASIVE CANDIDIASIS.
GB9912631D0 (en) * 1999-05-28 1999-07-28 Nat Blood Authority The Cell counter
KR20020032751A (en) * 2000-10-27 2002-05-04 김희태 A micro well plate protein chip using nitrocellulose membrane as a support and a method for the preparation thereof
GB0129776D0 (en) * 2001-12-13 2002-01-30 Sec Dep For Environment Food & Assay device and method
EP1599730A2 (en) * 2003-03-03 2005-11-30 Kouyama, Yoshihisa Methods and apparatus for use in detection and quantitation of various cell types and use of optical bio-disc for performing same
JP5144950B2 (en) * 2007-03-29 2013-02-13 株式会社バイオマーカーサイエンス Evaluation method of natural killer activity
US20200363406A1 (en) * 2017-10-26 2020-11-19 The University Of Houston System Highly-specific assays

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983002954A1 (en) * 1982-02-23 1983-09-01 Ventrex Lab Inc Multipurpose supports for immunological and biological use
US4471056A (en) * 1982-04-02 1984-09-11 The Board Of Trustees Of The Leland Stanford Junior University Method for HLA-DR typing of total human lymphocyte sample
US4606855A (en) * 1982-07-26 1986-08-19 Mex Research Associates C/O Leon Reimer Monoclonal antibody to digoxin
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
US4591570A (en) * 1983-02-02 1986-05-27 Centocor, Inc. Matrix of antibody-coated spots for determination of antigens
US4474892A (en) * 1983-02-16 1984-10-02 Board Of Trustees Of The Leland Stanford Junior University Two-site immunoassays using monoclonal antibodies of different classes or subclasses and test kits for performing same
US4599304A (en) * 1983-10-07 1986-07-08 Becton, Dickinson And Company Method for monitoring activated cell subpopulations
FR2571498B1 (en) * 1984-10-04 1988-04-08 Immunotech Sa METHOD FOR SEPARATING CELLS USING LOW DENSITY ANTIBODIES AND BALLS
US4677061A (en) * 1984-10-19 1987-06-30 Genetic Systems Corporation T-cell lymphocyte subset monitoring of immunologic disease
ATE106565T1 (en) * 1984-10-31 1994-06-15 Alcon Lab Inc SANDWICH IMMUNOTEST PROCEDURE FOR ANTIGENS IN OPHTHALMIC DISORDERS.
US4661446A (en) * 1985-02-19 1987-04-28 Dana-Farber Cancer Institute, Inc. Monoclonal antibodies and method of immunizing therewith
EP0204522A3 (en) * 1985-05-30 1987-09-16 Genetic Systems Corporation Monoclonal antibody panel for histocompatibility typing
WO1986007633A1 (en) * 1985-06-17 1986-12-31 Electro-Nucleonics, Inc. Test for determining false positive reactions in a testing procedure to detect the presence of antibody to microorganisms.
WO1987005398A1 (en) * 1986-02-26 1987-09-11 The University Of Melbourne Hla-b27 testing
AU7357687A (en) * 1986-04-29 1987-11-24 Murex Corp. Diagnostic kit for sexually transmitted diseases

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5385822A (en) * 1988-05-02 1995-01-31 Zynaxis, Inc. Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population
US5256532A (en) * 1988-05-02 1993-10-26 Zynaxis Technologies, Inc. Methods, reagents and test kits for determination of subpopulations of biological entities
AU642904B2 (en) * 1989-09-22 1993-11-04 La Mina Ltd Urine testing module and method of collecting urine antigen
WO1992009894A1 (en) * 1990-11-29 1992-06-11 Zynaxis Technologies, Incorporated Methods for detection and quantitation of cell subsets within subpopulations of a mixed cell population
US5646001A (en) * 1991-03-25 1997-07-08 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof
US5466574A (en) * 1991-03-25 1995-11-14 Immunivest Corporation Apparatus and methods for magnetic separation featuring external magnetic means
US5795470A (en) * 1991-03-25 1998-08-18 Immunivest Corporation Magnetic separation apparatus
US5500348A (en) * 1992-11-04 1996-03-19 Shionogi & Co., Ltd. Basophil-binding monoclonal antibody, method for separation of basophils, method for chemical mediator release from basophils, and method for testing release of basophil-derived chemical mediators
US5374531A (en) * 1993-03-22 1994-12-20 Zynaxis, Inc. Immunoassay for determination of cells
WO1994022013A1 (en) * 1993-03-22 1994-09-29 Zynaxis, Inc. Immunoassay for determination of cells
AU695012B2 (en) * 1993-03-22 1998-08-06 Intracel Corporation Immunoassay for determination of cells
US7005271B1 (en) 1994-07-23 2006-02-28 Jean-Marie Freyssinet Method for the determination of the prethrombotic state
US5658745A (en) * 1995-02-17 1997-08-19 E. I. Du Pont De Nemours And Company Cell enumeration immunoassay
WO1996027132A1 (en) * 1995-02-28 1996-09-06 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof

Also Published As

Publication number Publication date
NO177444C (en) 1995-09-13
IE882939L (en) 1989-03-30
FR2621128A1 (en) 1989-03-31
JPH01121755A (en) 1989-05-15
FI884472A (en) 1989-03-31
FR2621128B1 (en) 1994-05-06
KR890005518A (en) 1989-05-15
FI95752C (en) 1996-03-11
CA1340973C (en) 2000-04-25
IL87882A (en) 1993-01-14
DK174032B1 (en) 2002-04-29
FI95752B (en) 1995-11-30
NO884327L (en) 1989-03-31
KR0126236B1 (en) 1997-12-24
DE3888779T2 (en) 1994-09-08
IL87882A0 (en) 1989-03-31
NO884327D0 (en) 1988-09-29
DE3888779D1 (en) 1994-05-05
HK1001570A1 (en) 1998-06-26
IE63426B1 (en) 1995-04-19
FI884472A0 (en) 1988-09-29
PT88615A (en) 1989-07-31
EP0311492A2 (en) 1989-04-12
ES2053785T3 (en) 1994-08-01
DK550288A (en) 1989-03-31
EP0311492A3 (en) 1989-05-31
PT88615B (en) 1993-07-30
AU621310B2 (en) 1992-03-12
DK550288D0 (en) 1988-09-30
ATE103711T1 (en) 1994-04-15
EP0311492B1 (en) 1994-03-30
NO177444B (en) 1995-06-06

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Legal Events

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HB Alteration of name in register

Free format text: SANOFI-SYNTHELABO

MK14 Patent ceased section 143(a) (annual fees not paid) or expired