RU99110682A - Способ диагностики туберкулеза - Google Patents
Способ диагностики туберкулезаInfo
- Publication number
- RU99110682A RU99110682A RU99110682/14A RU99110682A RU99110682A RU 99110682 A RU99110682 A RU 99110682A RU 99110682/14 A RU99110682/14 A RU 99110682/14A RU 99110682 A RU99110682 A RU 99110682A RU 99110682 A RU99110682 A RU 99110682A
- Authority
- RU
- Russia
- Prior art keywords
- tuberculosis
- dna
- amplification
- cycles
- buffer
- Prior art date
Links
- 201000008827 tuberculosis Diseases 0.000 title claims 2
- 230000003321 amplification Effects 0.000 claims 3
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 241000187479 Mycobacterium tuberculosis Species 0.000 claims 2
- 229940010383 Mycobacterium tuberculosis Drugs 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 2
- 241000186366 Mycobacterium bovis Species 0.000 claims 1
- 229940114179 Mycobacterium bovis Drugs 0.000 claims 1
- FPKOPBFLPLFWAD-UHFFFAOYSA-N Trinitrotoluene Chemical compound CC1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1[N+]([O-])=O FPKOPBFLPLFWAD-UHFFFAOYSA-N 0.000 claims 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Tris Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 1
- 239000007984 Tris EDTA buffer Substances 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 claims 1
- 101710002867 ins-5 Proteins 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 229920000136 polysorbate Polymers 0.000 claims 1
Claims (1)
- Способ диагностики туберкулеза (Mycobacterium tuberculosis и Mycobacterium bovis), в котором применяют полимеразную цепную реакцию (Н-ПЦР), отличающийся тем, что ДНК выделяют в ТЕ-буфере с 1% тритоном, внешние праймеры и условия амплификации для каждого из этапов подобраны самостоятельно и представляют собой: INS5 -5'ggt ttg cgg tgg ggt gtcg 3' и INS6- 5' gtt gga tgc ctg cct cgg 3', амплификацию проводят в два этапа, температура отжига для первого этапа 60° (20 циклов) и температура отжига для второго этапа 72° (20 циклов), в Трис НСl, буфере с КСl, формамидом и твином, затем с помощью электрофореза разделяют продукты амплификации и под ультрафиолетовой лампой выявляют ярко-оранжевую полоску - ДНК образца на уровне ярко-оранжевой полоски - ДНК Mycobacterium tuberculosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU99110682A RU2163022C1 (ru) | 1999-05-28 | 1999-05-28 | Способ диагностики туберкулеза |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU99110682A RU2163022C1 (ru) | 1999-05-28 | 1999-05-28 | Способ диагностики туберкулеза |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2163022C1 RU2163022C1 (ru) | 2001-02-10 |
| RU99110682A true RU99110682A (ru) | 2001-04-20 |
Family
ID=20220158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU99110682A RU2163022C1 (ru) | 1999-05-28 | 1999-05-28 | Способ диагностики туберкулеза |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2163022C1 (ru) |
-
1999
- 1999-05-28 RU RU99110682A patent/RU2163022C1/ru active
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