RU99110373A - USE OF ANTI-BODIES AGAINST EMBRYONAL HEMOGLOBIN FOR IDENTIFICATION OF FETAL CELLS - Google Patents

USE OF ANTI-BODIES AGAINST EMBRYONAL HEMOGLOBIN FOR IDENTIFICATION OF FETAL CELLS

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Publication number
RU99110373A
RU99110373A RU99110373/14A RU99110373A RU99110373A RU 99110373 A RU99110373 A RU 99110373A RU 99110373/14 A RU99110373/14 A RU 99110373/14A RU 99110373 A RU99110373 A RU 99110373A RU 99110373 A RU99110373 A RU 99110373A
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RU
Russia
Prior art keywords
antibody
fetal
cells
blood sample
fragment
Prior art date
Application number
RU99110373/14A
Other languages
Russian (ru)
Other versions
RU2178703C2 (en
Inventor
Митчелл ГОЛБАС
Original Assignee
Эпплайд Имэджин, Инк.
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Priority claimed from US08/734,556 external-priority patent/US5731156A/en
Application filed by Эпплайд Имэджин, Инк. filed Critical Эпплайд Имэджин, Инк.
Publication of RU99110373A publication Critical patent/RU99110373A/en
Application granted granted Critical
Publication of RU2178703C2 publication Critical patent/RU2178703C2/en

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Claims (22)

1. Способ идентификации эритроцита или эритробласта плода в образце крови, взятой у беременной женщины в стадии от 9 до 22 недель беременности, при этом указанный способ включает в себя:
а) контакт образца крови с антителом или фрагментом антитела против эпсилон-глобиновой цепи эмбрионального гемоглобина, при этом антитело или его фрагмент представляет собой первый плодный маркер, и при этом антитело или его фрагмент связываются с плодной клеткой;
б) идентификацию клеток, которые связались с антителом или его фрагментом в качестве плодных эритроцитарных или эритробластных клеток.1. A method of identifying an erythrocyte or erythroblast of the fetus in a blood sample taken from a pregnant woman in the stage from 9 to 22 weeks of gestation, with this method includes:
a) contact of a blood sample with an antibody or antibody fragment against the epsilon-globin chain of the fetal hemoglobin, wherein the antibody or its fragment is the first fetal marker, and the antibody or its fragment is associated with the fetal cell;
b) identification of cells that are associated with the antibody or its fragment as fetal red blood cells or erythroblastic cells. 2. Способ по п.1, отличающийся тем, что образец крови представляет собой материнскую кровь человека и плодный эритроцит представляет собой содержащий ядро эритроцит. 2. The method according to claim 1, wherein the blood sample is the human maternal blood and the fetal erythrocyte is the nucleus-containing erythrocyte. 3. Способ по п.1, .отличающийся тем, что антитело представляет собой поликлональное антитело. 3. The method according to claim 1, characterized in that the antibody is a polyclonal antibody. 4. Способ по п. 1, отличающийся тем, что антитело представляет собой моноклональное антитело. 4. The method according to p. 1, characterized in that the antibody is a monoclonal antibody. 5. Способ по п. 1, отличающийся тем, что указанное антитело прямо или опосредованно помечено. 5. The method according to p. 1, characterized in that the antibody is directly or indirectly marked. 6. Способ, по п. 1, включающий в себя также контакт образца крови со вторым плодным маркером. 6. The method according to claim 1, which also includes the contact of the blood sample with the second fetal marker. 7. Способ по п.6, отличающийся тем, что второй плодный маркер представляет собой антитело или фрагмент этого антитела против гамма-глобиновой цепи плодного гемоглобина. 7. The method according to claim 6, wherein the second fetal marker is an antibody or a fragment of this antibody against the gamma-globin chain of fetal hemoglobin. 8. Способ по п. 1, включающий в себя контакт образца крови маркером зрелой клетки. 8. A method according to claim 1, comprising contacting a blood sample with a mature cell marker. 9. Способ по п.8, отличающийся тем, что маркер зрелой клетки представляет собой антитело или фрагмент этого антитела против бета-глобиновой цепи гемоглобина взрослого организма. 9. The method of claim 8, wherein the mature cell marker is an antibody or a fragment of this antibody against an adult beta-globin chain of hemoglobin. 10. Способ по п.1, отличающийся тем, что клетки крови находятся в суспензии. 10. The method according to claim 1, characterized in that the blood cells are in suspension. 11. Способ по п. 1, отличающийся тем, что образец крови высушивают на воздухе или химически фиксируют на твердой матрице перед или после контакта с антителом или его фрагментом. 11. The method according to p. 1, characterized in that the blood sample is dried in air or chemically fixed on a solid matrix before or after contact with the antibody or its fragment. 12. Способ по п.1, включающий в себя также обогащение концентрации плодных клеток в образце крови перед приведением их в контакт или его фрагментом, для идентификации плодных клеток. 12. The method according to claim 1, which also includes the enrichment of the concentration of fetal cells in the blood sample before bringing them into contact, or its fragment, to identify fetal cells. 13. Способ по п.12, отличающийся тем, что плодные клетки обогащены в результате применения проточной цитометрии, фракционирования крови, разделения в градиенте плотности и разделения с использованием магнитных шариков. 13. The method of claim 12, wherein the fetal cells are enriched by the use of flow cytometry, blood fractionation, density gradient separation and separation using magnetic beads. 14. Способ по п.1, включающий в себя также отбор или выделение нуклеиновой кислоты или белка, содержащихся в плодной клетке после проведения идентификации плодных клеток. 14. The method according to claim 1, which also includes the selection or isolation of nucleic acid or protein contained in the fetal cell after the identification of fetal cells. 15. Способ по п.14, отличающийся тем, что нуклеиновую кислоту или белок амплифицируют или выявляют. 15. The method of claim 14, wherein the nucleic acid or protein is amplified or detected. 16. Набор для идентификации плодного содержащего ядро эритроцита или эритробласта в образце крови, взятой у беременной женщины в стадии от 9 до 22 недель беременности, включающий в себя:
а) меченое антитело против эпсилон-глобиновой цепи эмбрионального гемоглобина;
б) инструкции по применению.16. A kit to identify a fetus containing the nucleus of an erythrocyte or erythroblast in a blood sample taken from a pregnant woman in the stage from 9 to 22 weeks of gestation, including:
a) labeled antibody against the epsilon-globin chain of embryonic hemoglobin;
b) instructions for use. 17. Набор по п.16, отличающийся тем, что антитело метят с помощью флуорохрома или фермента. 17. The kit according to claim 16, wherein the antibody is labeled with a fluorochrome or an enzyme. 18. Набор для идентификации плодного содержащего ядро эритроцита или эритробласта в образце крови, взятой у беременной женщины в стадии от 9 до 22 недель беременности, включающий в себя:
а) антитело против эпсилон-глобиновой цепи эмбрионального гемоглобина, меченное антигенным гаптеном;
б) меченое второе антитело против гаптена или против эпсилон-глобиновой цепи эмбрионального гемоглобина; и
в) инструкции по применению.18. A kit to identify a fetal nucleus containing an erythrocyte or erythroblast in a blood sample taken from a pregnant woman in the 9 to 22 week gestation stage, including:
a) an antibody against the epsilon-globin chain of embryonic hemoglobin, labeled with antigenic hapten;
b) labeled second antibody against the hapten or against the epsilon-globin chain of fetal hemoglobin; and
c) instructions for use. 19. Набор для идентификации плодного содержащего ядро эритроцита или эритробласта в образце крови, взятой у беременной женщины в стадии от 9 до 22 недель беременности, включающий в себя:
а) антитело против эпсилон-глобина эмбрионального гемоглобина, конъюгированное с биотином;
б) меченую молекулу авидина или стрептавидина; и
в) инструкции по применению.19. A kit to identify a fetus containing the nucleus of an erythrocyte or erythroblast in a blood sample taken from a pregnant woman in the stage from 9 to 22 weeks of gestation, including:
a) an anti-Epsilon-globin embryonic hemoglobin antibody conjugated with biotin;
b) labeled avidin or streptavidin molecule; and
c) instructions for use. 20. Набор по п.19, включающий также:
а) пробирку для фракционирования крови;
б) реагент для гемолиза;
в) среду с градиентом плотности.20. The kit according to claim 19, including also:
a) a tube for blood fractionation;
b) a hemolysis agent;
c) medium with a density gradient. 21. Набор для отбора или выделения последовательности нуклеиновой кислоты в плодном содержащем ядро эритроците или эритробласте, включающий в себя:
а) меченое антитело против эпсилон-глобиновой цепи эмбрионального гемоглобина, используемое для идентификации плодных клеток;
б) среду с градиентом плотности;
в) агент для лизирования плодных клеток после проведения идентификации указанных клеток;
г) меченый зонд нуклеиновый кислоты, специфичный в отношении отобранной или выделенной последовательности нуклеиновой кислоты;
д) инструкции по применению.21. A kit for selecting or isolating a nucleic acid sequence in a fetal nucleus erythrocyte or erythroblast, including:
a) a labeled antibody against the epsilon-globin chain of fetal hemoglobin, used to identify fetal cells;
b) medium with a density gradient;
c) an agent for lysing fetal cells after identifying these cells;
d) a labeled nucleic acid probe specific for a selected or isolated nucleic acid sequence;
e) instructions for use. 22. Набор по п.21, отличающийся тем, что меченый зонд нуклеиновой кислоты представляет собой зонд ДНК или РНК. 22. The kit according to claim 21, wherein the labeled nucleic acid probe is a DNA or RNA probe.
RU99110373/14A 1996-10-21 1997-10-20 Application of antibodies against embryonic hemoglobin to identify fetal cells RU2178703C2 (en)

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US08/734,556 US5731156A (en) 1996-10-21 1996-10-21 Use of anti-embryonic hemoglobin antibodies to identify fetal cells
US08/734,556 1996-10-21

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US (1) US5731156A (en)
EP (1) EP1007965B1 (en)
JP (1) JP4091123B2 (en)
KR (1) KR100523381B1 (en)
CN (1) CN1234117A (en)
AT (1) ATE290210T1 (en)
AU (1) AU735380B2 (en)
BR (1) BR9712548A (en)
CA (1) CA2269327A1 (en)
DE (1) DE69732662T2 (en)
HK (1) HK1031255A1 (en)
IL (1) IL129382A (en)
RU (1) RU2178703C2 (en)
WO (1) WO1998018005A1 (en)

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