CN109115584A - The detection reagent and its application of Hemoglobin F in a kind of detection red blood cell - Google Patents
The detection reagent and its application of Hemoglobin F in a kind of detection red blood cell Download PDFInfo
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- CN109115584A CN109115584A CN201811064880.3A CN201811064880A CN109115584A CN 109115584 A CN109115584 A CN 109115584A CN 201811064880 A CN201811064880 A CN 201811064880A CN 109115584 A CN109115584 A CN 109115584A
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- detection reagent
- solid
- red blood
- hemoglobin
- distilled water
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- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 210000003743 erythrocyte Anatomy 0.000 title claims abstract description 31
- 108091005880 Hemoglobin F Proteins 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000007787 solid Substances 0.000 claims abstract description 26
- 238000004043 dyeing Methods 0.000 claims abstract description 23
- 230000002378 acidificating effect Effects 0.000 claims abstract description 20
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims abstract description 18
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000012153 distilled water Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 10
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims abstract description 8
- 210000004369 blood Anatomy 0.000 claims description 26
- 239000008280 blood Substances 0.000 claims description 26
- 235000019441 ethanol Nutrition 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 102000010445 Lactoferrin Human genes 0.000 claims description 3
- 108010063045 Lactoferrin Proteins 0.000 claims description 3
- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 claims description 3
- 229940078795 lactoferrin Drugs 0.000 claims description 3
- 235000021242 lactoferrin Nutrition 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 210000001995 reticulocyte Anatomy 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 5
- 230000008774 maternal effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000010828 elution Methods 0.000 description 4
- 108010085682 Hemoglobin A Proteins 0.000 description 3
- 102000007513 Hemoglobin A Human genes 0.000 description 3
- MVEOHWRUBFWKJY-UHFFFAOYSA-N 7-hydroxynaphthalene-2-sulfonic acid Chemical compound C1=CC(S(O)(=O)=O)=CC2=CC(O)=CC=C21 MVEOHWRUBFWKJY-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical class [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229940069428 antacid Drugs 0.000 description 1
- 239000003159 antacid agent Substances 0.000 description 1
- 230000001458 anti-acid effect Effects 0.000 description 1
- 208000003464 asthenopia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to the applications of the detection reagent and the detection reagent of Hemoglobin F in a kind of detection red blood cell, the detection reagent includes acidic effluent liquid and dyeing liquor, and the acidic effluent liquid is prepared by following raw material: 25% hydrochloric acid solution, hematoxylin solid, ferric trichloride solid, 95% ethanol solution, distilled water;The dyeing liquor is prepared by following raw material: Yihong solid, distilled water.Detection reagent preparation is simple, and recall rate is high.
Description
Technical field
The present invention relates to a kind of detection reagent, in particular in a kind of detection red blood cell the detection reagent of Hemoglobin F with
And the application of the detection reagent.
Background technique
In recent years, tire mother transfusion syndrome gradually caused the attention of ob-gyn, how to early diagnose out this hair
Emphasis disease concealment and the disease of serious consequence is easily caused to pay close attention to jointly as ob-gyn and clinical laboratory doctor.Due to normal
99.9% red blood cell contains hemoglobin A without Hemoglobin F in adult human body, and red blood cell contains blood red egg in fetus body
White F does not contain hemoglobin A, therefore can be by Hemoglobin F content in detection pregnant and lying-in women's body, between judging whether that generation tire is female
The case where blood transfusion.Known Hemoglobin F has stronger capacity antacid than hemoglobin A.Therefore, the Blood piece after fixation, sets
Held for some time in acidic buffer, the only red blood cell containing Hemoglobin F are not eluted, such as with eosin stains in scarlet
Color.Hemoglobin F acid elution test (K-B experiment) is fetus in the most common quantitative detection maternal blood circulation of current each hospital
Red blood cell number purpose method.Hemoglobin is carried out according to the method for " national clinical examination operating instruction " (third edition) defined
F acid elution test finds influence of this method result vulnerable to external factors such as temperature, manual operation factors, and when testing
Between it is longer.And this law is ineffective for the red blood cell elution without containing Hemoglobin F, causes to judge whether red blood cell contains
The differentiation of Hemoglobin F result is unobvious, and as a result accuracy is poor.
Summary of the invention
The purpose of the present invention is to provide a kind of detection reagents of Hemoglobin F in new detection red blood cell, on solving
State the problem of proposing in background technique.
To achieve the above object, the invention provides the following technical scheme:
The detection reagent of Hemoglobin F in a kind of detection red blood cell, including acidic effluent liquid and dyeing liquor;The acidity is washed
De- liquid is prepared by following raw material: 25% hydrochloric acid solution, hematoxylin solid, ferric trichloride solid, 95% ethanol solution, distilled water;
The dyeing liquor is prepared by following raw material: Yihong solid, distilled water;The acidic effluent liquid the preparation method is as follows: by 0.75
Gram hematoxylin solid is completely dissolved in 100 milliliter of 95% ethanol solution, mixes, and mixture A is made;2.4 grams of ferric trichlorides are consolidated
Body is completely dissolved in 2 milliliter of 25% hydrochloric acid solution, and distilled water is added later to 100 milliliters, mixes, mixture B is made;Mixture
A and mixture B is uniformly mixed according to 5: 1 ratio of volume ratio, obtains the acidic effluent liquid;The preparation method of the dyeing liquor
It is as follows: 1 gram of Yihong solid being completely dissolved in 100 milliliters of distilled water, mixes, obtains the dyeing liquor.
A kind of application of detection reagent as described above, its step are as follows: (1) Blood piece with 80% ethyl alcohol is fixed 5 minute,
The Blood piece fixed is placed in 40 DEG C -50 DEG C of the acidic effluent liquid and keeps the temperature 1 minute, washed to dry;It (2) will be after drying
Blood piece is dyed 1 minute with the dyeing liquor, is washed to dry;(3) Blood piece after drying is placed under microscope, is knitted referring to net red thin
Born of the same parents' method of counting counts percentage shared by Hemoglobin F positive red blood cell in 1000 red blood cells.
A kind of purposes of detection reagent as described above, the detection reagent be used to detect Hemoglobin F in red blood cell
In shared percentage.
Compared with prior art, the beneficial effects of the present invention are: detection reagent of the invention is to without containing Hemoglobin F
Red blood cell eluting power is stronger, so that whether there is or not Hemoglobin F fruiting areas to show clearly for red blood cell, improves observer and is easy visual fatigue
Situation, convenient for counting under microscope, count results accuracy is good.Also, Hemoglobin F is carried out with detection reagent of the invention
Acid elution test is substantially shorter test period, reduces operating procedure, while reducing extraneous factor probability of interference, convenient for experiment
Room quickly checks.
Detailed description of the invention
In order to illustrate more clearly of technical solution of the present invention, the present invention is made with reference to the accompanying drawings and examples further
Explanation.
Attached drawing 1 show the colored graph for detecting Hemoglobin F in maternal blood using the detection reagent of embodiment 1
Piece;
Attached drawing 2 show the colored graph for detecting Hemoglobin F in maternal blood using the detection reagent of embodiment 2
Piece.
Specific embodiment
Below in conjunction with embodiment, to technical solution of the present invention carry out it is clear, be fully described by.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts,
It shall fall within the protection scope of the present invention.
Embodiment 1
In the present embodiment, a kind of detection reagent detecting Hemoglobin F in red blood cell, including acidic effluent liquid and dyeing
Liquid;The acidic effluent liquid is prepared by following raw material: 25% hydrochloric acid solution, hematoxylin solid, ferric trichloride solid, 95% ethyl alcohol
Solution, distilled water;The dyeing liquor is prepared by following raw material: Yihong solid, distilled water.
Preparation method includes the following steps:
(1) preparation of mixed liquor A: 0.75 gram of hematoxylin solid is sufficiently dissolved with 100 milliliter of 95% ethanol solution, is mixed
It is even;
(2) it the preparation of mixed liquid B: after 2.4 grams of ferric trichloride solids are dissolved in 2 milliliter of 25% hydrochloric acid solution, are added and steam
Distilled water is mixed to 100 milliliters;
(3) preparation of acidic effluent liquid: by mixed liquor A and mixed liquid B, 5: 1 ratios are uniformly mixed by volume;Acidity is washed
The pH value of de- liquid is 1.65 ± 0.2;
(4) preparation of dyeing liquor: 1 gram of Yihong solid is dissolved in 100 milliliters of distilled water, is mixed.
Detection reagent described in the invention uses step:
(1) Blood piece is fixed 5 minutes with 80% ethyl alcohol, it is accurate the Blood piece fixed to be placed in 40 DEG C of acidic effluent liquid
After heat preservation 1 minute, wash to dry;
(2) it after dyeing the Blood piece after drying 1 minute with dyeing liquor, washes to dry;
(3) Blood piece after drying is placed under microscope, counts 1000 red blood cells referring to reticulocyte count method
Percentage shared by middle Hemoglobin F positive red blood cell.
Embodiment 2
In the present embodiment, a kind of detection reagent detecting Hemoglobin F in red blood cell, including acidic effluent liquid and dyeing
Liquid;The acidic effluent liquid is prepared by following raw material: 25% hydrochloric acid solution, hematoxylin solid, ferric trichloride solid, 95% ethyl alcohol
Solution, distilled water;The dyeing liquor is prepared by following raw material: Yihong solid, distilled water.
Preparation method includes the following steps:
(1) preparation of mixed liquor A: 0.75 gram of hematoxylin solid is sufficiently dissolved with 100 milliliter of 95% ethanol solution, is mixed
It is even;
(2) it the preparation of mixed liquid B: after 2.4 grams of ferric trichloride solids are dissolved in 2 milliliter of 25% hydrochloric acid solution, are added and steam
Distilled water is mixed to 100 milliliters;
(3) preparation of acidic effluent liquid: by mixed liquor A and mixed liquid B, 5: 1 ratios are uniformly mixed by volume;Acidity is washed
The pH value of de- liquid is 1.65 ± 0.2;
(4) preparation of dyeing liquor: 1 gram of Yihong solid is dissolved in 100 milliliters of distilled water, is mixed.
Detection reagent described in the invention uses step:
(1) Blood piece is fixed 5 minutes with 80% ethyl alcohol, it is accurate the Blood piece fixed to be placed in 50 DEG C of acidic effluent liquid
After heat preservation 1 minute, wash to dry;
(2) it after dyeing the Blood piece after drying 1 minute with dyeing liquor, washes to dry;
(3) Blood piece after drying is placed under microscope, counts 1000 red blood cells referring to reticulocyte count method
Percentage shared by middle Hemoglobin F positive red blood cell.
Detection reagent made from above-described embodiment 1-2 is applied to red blood cell of the observation containing Hemoglobin F.
Observe result: the red blood cell containing Hemoglobin F is colored liquid and dyes cerise, is positive cell, does not contain blood
The red blood cell of Lactoferrin F only leaves the cell membrane person of light shadow, for negative red blood cell.
It is that Hemoglobin F is detected in maternal blood using the detection reagent in above-described embodiment 1 shown in attached drawing 1
Picture is dyed, wherein dark cell is positive cell.It is to be existed shown in attached drawing 2 using the detection reagent in above-described embodiment 2
The dyeing picture of Hemoglobin F is detected in maternal blood, wherein dark cell is positive cell.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, this is fallen in
All changes in the meaning and scope of invention the meaning and scope of the equivalent requirements of the claims are included within the present invention.
Claims (3)
1. the detection reagent of Hemoglobin F in a kind of detection red blood cell, including acidic effluent liquid and dyeing liquor;
The acidic effluent liquid is prepared by following raw material: 25% hydrochloric acid solution, hematoxylin solid, ferric trichloride solid, 95% second
Alcoholic solution, distilled water;
The dyeing liquor is prepared by following raw material: Yihong solid, distilled water;
The acidic effluent liquid the preparation method is as follows: 0.75 gram of hematoxylin solid is completely dissolved in 100 milliliter of 95% ethyl alcohol
Solution mixes, and mixture A is made;2.4 grams of ferric trichloride solids are completely dissolved in 2 milliliter of 25% hydrochloric acid solution, are added later
Distilled water is mixed to 100 milliliters, and mixture B is made;Mixture A and mixture B is uniformly mixed according to 5: 1 ratio of volume ratio, i.e.,
The acidic effluent liquid is made;
The dyeing liquor the preparation method is as follows: 1 gram of Yihong solid is completely dissolved in 100 milliliters of distilled water, mix, that is, make
Obtain the dyeing liquor.
2. a kind of application of detection reagent as described in claim 1, which is characterized in that steps are as follows:
(1) Blood piece is fixed 5 minutes with 80% ethyl alcohol, the Blood piece fixed is placed in 40 DEG C -50 DEG C of the acidic effluent liquid
Middle heat preservation 1 minute, is washed to dry;
(2) dyeing liquor of the Blood piece after drying is dyed 1 minute, is washed to dry;
(3) Blood piece after drying is placed under microscope, counts blood in 1000 red blood cells referring to reticulocyte count method
Percentage shared by Lactoferrin F positive red blood cell.
3. a kind of purposes of detection reagent as described in claim 1, which is characterized in that the detection reagent be used to detect blood
Lactoferrin F shared percentage in red blood cell.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811064880.3A CN109115584A (en) | 2018-09-10 | 2018-09-10 | The detection reagent and its application of Hemoglobin F in a kind of detection red blood cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811064880.3A CN109115584A (en) | 2018-09-10 | 2018-09-10 | The detection reagent and its application of Hemoglobin F in a kind of detection red blood cell |
Publications (1)
Publication Number | Publication Date |
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CN109115584A true CN109115584A (en) | 2019-01-01 |
Family
ID=64859178
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201811064880.3A Pending CN109115584A (en) | 2018-09-10 | 2018-09-10 | The detection reagent and its application of Hemoglobin F in a kind of detection red blood cell |
Country Status (1)
Country | Link |
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CN (1) | CN109115584A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1234117A (en) * | 1996-10-21 | 1999-11-03 | 应用成像公司 | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
US20030157113A1 (en) * | 1999-12-28 | 2003-08-21 | Terman David S. | Compositions and methods for treatment of neoplastic disease |
-
2018
- 2018-09-10 CN CN201811064880.3A patent/CN109115584A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1234117A (en) * | 1996-10-21 | 1999-11-03 | 应用成像公司 | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
US20030157113A1 (en) * | 1999-12-28 | 2003-08-21 | Terman David S. | Compositions and methods for treatment of neoplastic disease |
Non-Patent Citations (1)
Title |
---|
SAMUEL S. ANTWI-BAFFOUR ET AL: "Foetomaternal Haemorrhage (FMH): A Case for Routine Screening", 《JOURNAL OF GYNECOLOGY AND OBSTETRICS,》 * |
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