RU98104924A - Isolation of Clavanic Acid from the Fermentation Bouillon by Ultrafiltration - Google Patents

Isolation of Clavanic Acid from the Fermentation Bouillon by Ultrafiltration

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Publication number
RU98104924A
RU98104924A RU98104924/04A RU98104924A RU98104924A RU 98104924 A RU98104924 A RU 98104924A RU 98104924/04 A RU98104924/04 A RU 98104924/04A RU 98104924 A RU98104924 A RU 98104924A RU 98104924 A RU98104924 A RU 98104924A
Authority
RU
Russia
Prior art keywords
ultrafiltration
filtrate
clavulanic acid
isolation
ester
Prior art date
Application number
RU98104924/04A
Other languages
Russian (ru)
Other versions
RU2169151C2 (en
Inventor
Эгидий Цапудер
Original Assignee
Лек Фармасьютикал энд Кемикал Ко. ДД
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SI9500265A external-priority patent/SI9500265A1/en
Application filed by Лек Фармасьютикал энд Кемикал Ко. ДД filed Critical Лек Фармасьютикал энд Кемикал Ко. ДД
Publication of RU98104924A publication Critical patent/RU98104924A/en
Application granted granted Critical
Publication of RU2169151C2 publication Critical patent/RU2169151C2/en

Links

Claims (9)

1. Способ получения и/или очистки клавулановой кислоты или ее фармацевтически приемлемой соли или сложного эфира, включающий в себя удаление твердых веществ из содержащего клавулановую кислоту ферментационного бульона посредством микрофильтрации с образованием первого фильтрата, дальнейшее удаление твердых веществ из первого фильтрата посредством ультрафильтрации с образованием второго фильтрата, концентрирование второго фильтрата путем удаления воды и обработку концентрированного второго фильтрата с выделением клавулановой кислоты или ее фармацевтически приемлемой соли или сложного эфира, отличающийся тем, что первый фильтрат фильтруют с использованием мембраны для ультрафильтрации, имеющей отсечку молекулярной массы от 10 до 30 кДа, и пропускающую способность от 10 до 30 л•м-2•ч1.1. A method for producing and / or purifying clavulanic acid or a pharmaceutically acceptable salt or ester thereof, comprising removing solids from clavulanic acid-containing fermentation broth by microfiltration to form a first filtrate, further removing solids from the first filtrate by ultrafiltration to form a second filtrate, concentrating the second filtrate by removing water and treating the concentrated second filtrate with isolation of clavulanic acid lot or its pharmaceutically acceptable salt or ester, characterized in that the first filtrate is filtered using an ultrafiltration membrane having a molecular weight cut-off of 10 to 30 kDa and a transmittance of 10 to 30 l • m -2 • h 1 . 2. Способ по п.1, отличающийся тем, что рН первого фильтрата перед ультрафильтрацией составляет от 5,8 до 6,2. 2. The method according to claim 1, characterized in that the pH of the first filtrate before ultrafiltration is from 5.8 to 6.2. 3. Способ по п.1 или 2, отличающийся тем, что ультрафильтрация является непрерывной. 3. The method according to claim 1 or 2, characterized in that the ultrafiltration is continuous. 4. Способ по п. 3, отличающийся тем, что непрерывную ультрафильтрацию проводят с использованием двухступенчатого аппарата. 4. The method according to p. 3, characterized in that continuous ultrafiltration is carried out using a two-stage apparatus. 5. Способ по любому из пп.1-4, отличающийся тем, что мембрана для ультрафильтрации содержит полисульфон. 5. The method according to any one of claims 1 to 4, characterized in that the ultrafiltration membrane contains polysulfone. 6. Способ по любому из пп.1-5, отличающийся тем, что пропускающая способность мембраны составляет 12 л•м-2•ч-1.6. The method according to any one of claims 1 to 5, characterized in that the transmittance of the membrane is 12 l • m -2 • h -1 . 7. Способ по любому из пп.1-6, отличающийся тем, что отсечка молекулярной массы составляет 20 кДа. 7. The method according to any one of claims 1 to 6, characterized in that the molecular weight cut-off is 20 kDa. 8. Способ получения и/или очистки клавулановой кислоты или ее фармацевтически приемлемой соли или сложного эфира, отличающийся тем, что сокращают количество примесей, имеющих поглощение при 420 нм. 8. The method of obtaining and / or purification of clavulanic acid or its pharmaceutically acceptable salt or ester, characterized in that they reduce the amount of impurities having an absorption at 420 nm. 9. Способ по п.8, включающий в себя стадии, которые определены по любому из пп. 1-7. 9. The method of claim 8, including the stages that are defined according to any one of paragraphs. 1-7.
RU98104924/04A 1995-08-28 1996-08-27 Method of preparing and/or purification of clavulanic acid from fermentation broth by ultrafiltration RU2169151C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SIP-9500265 1995-08-28
SI9500265A SI9500265A1 (en) 1995-08-28 1995-08-28 Process for purification of the aqueous fermented broth filtrate of streptomyces sp. p 6621 ferm p 2804 by ultrafiltration

Publications (2)

Publication Number Publication Date
RU98104924A true RU98104924A (en) 1999-12-10
RU2169151C2 RU2169151C2 (en) 2001-06-20

Family

ID=20431679

Family Applications (1)

Application Number Title Priority Date Filing Date
RU98104924/04A RU2169151C2 (en) 1995-08-28 1996-08-27 Method of preparing and/or purification of clavulanic acid from fermentation broth by ultrafiltration

Country Status (20)

Country Link
US (2) US6127358A (en)
EP (1) EP0859780A1 (en)
JP (1) JP2000512125A (en)
KR (1) KR19990035966A (en)
CN (1) CN1101396C (en)
AU (1) AU714892B2 (en)
BG (1) BG63086B1 (en)
CA (1) CA2226683C (en)
CZ (1) CZ31898A3 (en)
EE (1) EE03567B1 (en)
HU (1) HU221353B1 (en)
NZ (1) NZ316136A (en)
PL (1) PL324855A1 (en)
RO (1) RO116813B1 (en)
RU (1) RU2169151C2 (en)
SI (1) SI9500265A1 (en)
SK (1) SK10398A3 (en)
UA (1) UA59343C2 (en)
WO (1) WO1997008175A1 (en)
ZA (1) ZA967273B (en)

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