RU98103368A

RU98103368A - INDUCED HERBICIDAL STABILITY

Info

Publication number: RU98103368A
Application number: RU98103368/13A
Authority: RU
Inventors: Джепсон Ян
Original assignee: Зенека Лимитед
Priority date: 1995-08-03
Filing date: 1996-08-02
Publication date: 2000-01-10

Claims

1. A chemically inducible gene expression cassette comprising an inducible promoter operably linked to a target gene that confers resistance to the herbicide, where the target gene confers resistance to the herbicide N-phosphonomethylglycine or a salt or derivative thereof.

2. The chemically inducible gene expression cassette of claim 1, wherein the herbicide is N-phosphonomethylglycine or a salt thereof or a derivative thereof.

3. The chemically induced gene expression cassette according to claim 1 or 2, wherein the inducible promoter is a tetracycline repressor switch gene, a Lac repressor system, copper-induced systems such as those based on ACE 1, salicylic acid-induced promoters, for example PR-la systems, systems based on a steroid hormone such as glucocorticoid, progesterone and estrogen systems, or a modification of one of them, such as glucocorticoid receptor system, which includes a GAL 4-binding ene from yeast and VP 16 activator are insect steroid hormones systems such as those based on the ecdysone receptor of Heliothis virescens, a gene switching system benzenesulfonamide, a gene switching based on the alcR-protein from Aspergillus nidulans, or promoter glutathione transferase-S-.

4. A chemically inducible gene expression cassette comprising a first promoter operably linked to an alcR regulatory sequence that encodes an alcR regulatory protein and an inducible promoter operably linked to a target gene that confers herbicidal resistance, wherein the inducible promoter is activated by regulatory protein in the presence of an effective exogenous inducer, whereby, the use of an inducer induces expression of the target gene.

5. The gene expression cassette of claim 4, wherein the inducible promoter is derived from a1cA, alcR, aldA, or another alcR-inducible gene promoter.

6. Gene expression cassette according to any one of paragraphs. 4 and 5, where the inducible promoter is a chimeric promoter.

7. A plant cell containing the gene expression cassette according to any preceding paragraph.

8. The plant cell according to claim 7, where the gene expression cassette is stably incorporated into the genome of the plant.

9. A method for controlling herbicidal resistance, comprising transforming a plant cell using a plant gene expression cassette according to any one of claims. 1-6.

10. The method of selective control of weeds among field plants, including a plant cell according to any one of paragraphs. 7 and 8, including the application in the field of an effective amount of the indicated herbicide and exogenous inducer.

11. Gene expression cassette comprising a first promoter operably linked to a first target gene encoding a product that confers resistance to N-phosphonomethylglycine; and a second promoter operably linked to a second target gene encoding a product that metabolizes N-phosphonomethylglycine; moreover, the second promoter is inducible as a result of external use of an effective exogenous inducer, and where the plant's resistance to N-phosphonomethylglycine is provided by expression of the first and / or second target genes.

12. The gene expression cassette of claim 11, wherein said inducible promoter is derived from alcA, alcR, aldA, or from another alcR-inducible gene promoter.

13. The gene expression cassette of claim 11 or 12, wherein the first promoter is a constitutive promoter.

14. Gene expression cassette, where the first target gene encodes 5-enolpyruvyl-chymate-3-phosphate (EPSPS) CP4.

15. Gene expression cassette, where the second target gene encodes glyphosate oxidase.