RU95110112A - Method of preparing chitosan-glucane complex
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Method of preparing chitosan-glucane complex
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RU95110112A
RU95110112ARU95110112/13ARU95110112ARU95110112ARU 95110112 ARU95110112 ARU 95110112ARU 95110112/13 ARU95110112/13 ARU 95110112/13ARU 95110112 ARU95110112 ARU 95110112ARU 95110112 ARU95110112 ARU 95110112A
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Application filed by Акционерное общество закрытого типа "Цитробел"filedCriticalАкционерное общество закрытого типа "Цитробел"
Priority to RU95110112ApriorityCriticalpatent/RU2121505C1/en
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Publication of RU2121505C1publicationCriticalpatent/RU2121505C1/en
FIELD: biotechnology. SUBSTANCE: invention proposes method of preparing chitosan-glucane complex at content of glucane and chitosan from 25 to 60% and deacetylation degree from 15 to 70%. Biomass of Aspergillus niger is treated with 2 N hydrochloric acid, then - with a mixture of reagents (detergent, weak alkali, ethanol) at 96 C for 35-45 min. Deacetylation is carried out with 20-25% alkali for 1-2 h at 100-110 C. Then the end product is washed off with alcohols to remove alkali at decreasing concentration and with water. EFFECT: accelerated and universal method.
Claims (1)
Описан способ получения хитозан-глюканового комплекса с содержанием глюкана и хитозана от 25 до 60% и степенью деацетилирования от 15 до 70%. Биомассу Aspergillus niger обрабатывают 2н соляной кислотой, далее смесью реагентов ( детергентом, слабой щелочью, этанолом) при 96oС в течение 35-45 мин. Деацетилирование ведут 20-25%-ной щелочью в течение 1-2 ч при температуре 100-110oС. Далее целевой продукт отмывают от щелочи спиртами с понижающимися концентрациями и водой. Предлагаемый способ получения хитозан-глюканового комплекса является по сравнению с аналогами более быстрым, универсаль- ным и экологически более чистым.A method for producing a chitosan-glucan complex with a glucan and chitosan content of 25 to 60% and a deacetylation degree of 15 to 70% is described. The Aspergillus niger biomass is treated with 2N hydrochloric acid, followed by a mixture of reagents (detergent, weak alkali, ethanol) at 96 ° C for 35-45 minutes. Deacetylation is carried out with 20-25% alkali for 1-2 hours at a temperature of 100-110 o C. Next, the target product is washed from alkali with alcohols with lower concentrations and water. The proposed method for producing the chitosan-glucan complex is faster, more versatile and more environmentally friendly in comparison with analogues.
RU95110112A1995-06-151995-06-15Method of chitosan-glucane complex preparing
RU2121505C1
(en)