RU94017790A - Method of preparing sowing material for citric acid production
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Method of preparing sowing material for citric acid production
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RU94017790A
RU94017790ARU94017790/13ARU94017790ARU94017790ARU 94017790 ARU94017790 ARU 94017790ARU 94017790/13 ARU94017790/13 ARU 94017790/13ARU 94017790 ARU94017790 ARU 94017790ARU 94017790 ARU94017790 ARU 94017790A
Государственный научно-исследовательский институт биосинтеза белковых веществ
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Priority to RU94017790ApriorityCriticalpatent/RU2092557C1/en
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Preparation Of Compounds By Using Micro-Organisms
(AREA)
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof
(AREA)
Abstract
FIELD: biotechnology. SUBSTANCE: method involves the use of the strain A. niger. Strain is obtained by step-by-step treatment of parental strain with chemical mutagens, in part, N-nitrosomethylurea and nitrosonitromethylurea. The use of indicated strain A. niger at submerged cultivation promotes increase yield of citric acid (an average by 10%) and citric acid concentration in total synthesized acids by 15%. Also, strain can ferment molasses medium containing increased sugar content. EFFECT: increased yield of citric acid.
Claims (1)
С целью повышения выхода лимонной кислоты селекционирован штамм A.niger, характеризующийся более высокой, чем исходный штамм, активностью биосинтеза лимонной кислоты. Штамм получен путем ступенчатой обработки исходного штамма химическими мутагенами, в частности путем обработки N- нитрозометилмочевиной и нитрозонитрометилмочевиной. Использование посевного материала селекционированного штамма A.niger при глубинном культивировании способствует увеличению выхода лимонной кислоты в среднем на 10%, увеличению концентрации лимонной кислоты в сумме синтезируемых кислот в среднем на 15%. Кроме того, штамм обладает способностью ферментировать мелассную среду с более высоким содержанием сахара.In order to increase the yield of citric acid, A.niger strain was selected, which is characterized by higher than the original strain, activity of citric acid biosynthesis. The strain was obtained by stepwise treatment of the initial strain with chemical mutagens, in particular by treatment with N-nitrosomethylurea and nitrosonitromethylurea. The use of seed of the selected A.niger strain during deep cultivation contributes to an increase in the yield of citric acid by an average of 10%, and an increase in the concentration of citric acid in the sum of the synthesized acids by an average of 15%. In addition, the strain has the ability to ferment molasses medium with a higher sugar content.
RU94017790A1994-05-111994-05-11Method of preparing the sowing material for citric acid production
RU2092557C1
(en)
Strain of yeast schizosaccharomyces acidodevoratus vierul y-282 for biological deacidation of grape wort of wine and method for biologically deacidizing grape wort or wine