RU93040843A - METHOD FOR DETERMINING EXOTOXIN OF CORINE BACTERIA DIPHTERIA IN BLOOD OF PATIENTS WITH TOXIC FORM OF DIPHTERIA INFECTION - Google Patents

METHOD FOR DETERMINING EXOTOXIN OF CORINE BACTERIA DIPHTERIA IN BLOOD OF PATIENTS WITH TOXIC FORM OF DIPHTERIA INFECTION

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Publication number
RU93040843A
RU93040843A RU93040843/14A RU93040843A RU93040843A RU 93040843 A RU93040843 A RU 93040843A RU 93040843/14 A RU93040843/14 A RU 93040843/14A RU 93040843 A RU93040843 A RU 93040843A RU 93040843 A RU93040843 A RU 93040843A
Authority
RU
Russia
Prior art keywords
diphteria
exotoxin
patients
blood
determining
Prior art date
Application number
RU93040843/14A
Other languages
Russian (ru)
Other versions
RU2087911C1 (en
Inventor
О.А. Аксенов
А.Н. Сиземов
В.В. Иванова
Original Assignee
Научно-исследовательский институт детских инфекций, Санкт-Петербург
Filing date
Publication date
Application filed by Научно-исследовательский институт детских инфекций, Санкт-Петербург filed Critical Научно-исследовательский институт детских инфекций, Санкт-Петербург
Priority to RU93040843A priority Critical patent/RU2087911C1/en
Priority claimed from RU93040843A external-priority patent/RU2087911C1/en
Publication of RU93040843A publication Critical patent/RU93040843A/en
Application granted granted Critical
Publication of RU2087911C1 publication Critical patent/RU2087911C1/en

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Claims (1)

Способ относится к медицине, в частности к бактериологии. Способ основан на выявлении его (экзотоксина) в сыворотке крови больных в остром периоде заболевания до серотерапии. Авторы предлагают определять в сыворотке крови уровень эндогенного комплемента, затем разводить до содержания комплемента 1 - 2 гемолизирующих дозы, вносить 50 - 100 антитоксических доз противодифтерийной антитоксической сыворотки и через 1 ч инкубирования при 37oC определяют уровень задержки эндогенного комплемента по каталазной активности лизированных эритроцитов, вносимых в реакционную смесь в комплексе с 10 гемолитическими дозами антиэритроцитарной сыворотки.The method relates to medicine, in particular to bacteriology. The method is based on the identification of it (exotoxin) in the blood serum of patients in the acute period of the disease before serotherapy. The authors propose determining the level of endogenous complement in the blood serum, then diluting 1 to 2 hemolytic doses to complement content, adding 50 to 100 antitoxic doses of antidiphtheria antitoxic serum, and after 1 h of incubation at 37 o C, the level of endogenous complement retention is determined by the catalase activity of lysed red blood cells, introduced into the reaction mixture in combination with 10 hemolytic doses of anti-erythrocyte serum.
RU93040843A 1993-08-11 1993-08-11 Method for determining diphtheria corinebacteria exotoxin in patient blood suffering from toxic form of diphtheria infection RU2087911C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU93040843A RU2087911C1 (en) 1993-08-11 1993-08-11 Method for determining diphtheria corinebacteria exotoxin in patient blood suffering from toxic form of diphtheria infection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU93040843A RU2087911C1 (en) 1993-08-11 1993-08-11 Method for determining diphtheria corinebacteria exotoxin in patient blood suffering from toxic form of diphtheria infection

Publications (2)

Publication Number Publication Date
RU93040843A true RU93040843A (en) 1996-10-10
RU2087911C1 RU2087911C1 (en) 1997-08-20

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Family Applications (1)

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RU93040843A RU2087911C1 (en) 1993-08-11 1993-08-11 Method for determining diphtheria corinebacteria exotoxin in patient blood suffering from toxic form of diphtheria infection

Country Status (1)

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RU (1) RU2087911C1 (en)

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