RU41729U1 - PLANT FOR MIXING AND THERMOSTATING LIQUID IN FLASKS AND BOTTLES - Google Patents

Abstract

The utility model relates to equipment for the cultivation of microorganisms during bacteriological and serological studies in laboratories of clinics and hospitals, research institutes and other healthcare institutions. Installation for mixing and temperature control of the liquid in the vessels contains a heat chamber 1, inside of which are replaceable tables 2 with holders 3 for flasks and bottles. The thermal chamber 1 is suspended on a cable 4 through an anchor bolt 5 and connected to an external control unit 6. The thermal chamber 1 consists of an outer 7 and an inner 8 chambers, between which a heat insulator 9 is placed. The capacity of the inner chamber 8 is increased due to the special arrangement of holders 3 for flasks , bottles on removable tables 2: four holders 3 at the corners are perpendicular to the walls of the inner chamber 8, and the rest are at an acute angle and the ventilation holes 10 with shutters, respectively, in the lid 12 and at the bottom of the heat chamber 1. Inside In the lower chamber 8, an arc mercury lamp 13 (DRC 120) is installed for disinfection during prolonged temperature control, in the upper part there is a temperature sensor 14, and in the lower part there is an electric heating element 15. The electric motor 17 is attached outside the heat chamber 1 and turns on after closing the cover 12 of the heat chamber 1 when the limit switch 18 is activated. The amplitude and frequency of oscillations of the heat chamber 1 can be adjusted by an eccentric: an adjusting counterweight 19 on the flywheel 20 of the motor shaft 17 and the autotransformer 21 installed on and the control unit 6. On the remote control unit 6, a temperature controller 22 is installed for automatic regulation in p

Description

The utility model relates to equipment for the microbiological industry, namely, devices for cultivating microorganisms for conducting bacteriological and serological studies in clinical diagnostic and sanitary-bacteriological laboratories of clinics and hospitals, research institutes and other healthcare institutions.

There are many plants for growing microorganisms.

A known installation for the cultivation of microorganisms in which a pre-sterilized fermenter is introduced through a manifold and a pipe through a manifold and pipe a sterile nutrient medium, and then the seed is fed into it. With stirring and aeration, a foam forms in the fermenter, the penetration of which into the cavity is prevented by means of a perforated chipper with flanging. In cases where the foam level is above the permissible level, an antifoam is supplied through the pipeline. To maintain optimal cultivation conditions, the necessary additives are introduced into the fermenter through the collector via the pipeline [USSR Author's Certificate, No. 734256, class. C 12 M 1/02, dated July 22, 87].

The disadvantage of this installation is the impossibility of its use in laboratory conditions, the complexity of the design.

A device for growing microorganisms is known, in which a cylindrical container is filled with a nutrient medium and microorganisms are sown. Then the mixer is turned on and air is supplied through the aerator, and the screws are also rotated. The latter suck in the culture medium through the channels in the casings and discard through the channels. In this case, the medium is dispersed in the cavities of the casings, and the entire mass of the medium in the tank acquires a rotational motion, during which the medium is mixed

as a mixer and augers. The augers during their rotation pump fluid from the upper layers of the medium volume in the tank to the lower, to its bottom [Copyright certificate No. 371271, class. C 12 M 1/06, dated 07.02.89].

However, this device is suitable mainly for the industrial cultivation of microorganisms, requires qualified staff, cumbersome in size and not suitable for use in laboratory conditions.

A known installation of an electric heat chamber with a water jacket type - CVE-2, designed to store samples at a constant temperature in the range of ambient temperature up to + 70 ° C. The camera is used in laboratory conditions for growing bacterial cultures. The desired temperature is set on the contact thermometer, is automatically held in the inner cavity of the chamber [Technical documentation of the Electric heat chamber with a water jacket type CVE-2.-Lodz, Poland, 1979. - 8 p.].

The disadvantage of this installation is the constant control of the water level and the absence of a shaker for mixing and aeration of the nutrient medium and bacterial culture.

A device for shaking AVU-6P is known, intended for mechanically mixing liquids in flasks, test tubes, and bottles when growing crops. It consists of the following main units: frame, base, drive, linkage and removable tables. The drive of the apparatus is electromechanical, the number of oscillations is regulated within 100-300 per minute. The amplitude of the oscillations is regulated in the range from 10 to 25 mm [Prospect for the rules of operation of equipment for shaking. - Moscow, 1973 - S.10-11].

However, this device to maintain a certain temperature is placed in a special box and contains a limited number of flasks with shaking.

A known apparatus for growing microorganisms. The stimulator of the flow from the bottom of the overflow nozzle is to direct the processed fluid through the suction and discharge pipelines into the ejectors. In the ejectors, the processed fluid is mixed with the air ejected from the atmosphere and introduced into the apparatus in the form of an incident jet, which processes the entire layer of monolithic liquid in the apparatus. Upon contact of the jet with a device for distributing a gas-liquid flow, its flow is divided as follows. The main part of the gas-liquid mixture flow passes through a vertical cylinder - stream III, and the rest, cut off by the upper edges of the cylinder, falls on the upper surface of the disk, which is coaxially fixed to the outer surface of the cylinder and is reflected from it. The air released in this case, floating up, forms an upward flow II. The flow of gas-liquid mixture III, being under the disk, is evenly distributed along its lower surface in the direction of the periphery. Having reached the perforation zone, the gas component of stream III, evenly distributed in the volume of the apparatus, forms an upward stream IV. Next, the processed liquid enters the overflow cup and is sent to the next recycling cycle [Installations for industrial wastewater treatment. A review of the work of the NP "Chemianlangenbaukombinat", Leipzig, GDR, p.4-5].

However, this apparatus is a complex engineering system that requires a powerful fan and compressor and does not contain devices to maintain temperature.

A device is known for a bacteriological thermostat, providing the necessary thermal conditions for the cultivation of bacteria and viruses. The bacteriological thermostat consists of an external cabinet with thermal insulation, an internal cabinet with a channel for forced air circulation and heating. Electrical cabinet with mechanical and electrical control devices [Passport of bacteriological thermostat LP-120/2. Esztergom, Hungarian People's Republic, 1981. - 23 p.].

The disadvantage of this device is the lack of mechanisms for shaking the nutrient medium and the bacterial culture for aeration and preventing sticking.

A known device is a dry-air electric thermostat TS-80M-2, designed to obtain and maintain a stable temperature inside the working chamber, necessary for bacteriological and serological studies. The thermostat is equipped with a control unit that provides continuous temperature change in the working chamber of the thermostat and automatic maintenance of the set temperature [Passport for operating the thermostat of electric dry-air TS-80M-2.-Odessa, 1986. - 47 p.].

However, this device does not allow shaking of flasks with a nutrient medium and a bacterial culture.

Known installation - laboratory shaking machine WS-2, designed for continuous processes of mixing liquid. The electric motor rotates through the pulleys to a continuously variable friction gear. The shaker contains devices for attaching laboratory sieves, attaching a vessel with a capacity of more than 5 liters, for attaching tubes, flasks [Instructions for maintenance of a laboratory shaking machine. Warsaw, Poland, 1985. - 9 p.].

The disadvantage of this installation is the lack of devices to maintain a constant temperature.

The closest technical solution to the utility model is a shaker with a water bath - type 357, Elpan electronic equipment factory, Lubawa (Poland), consisting of an electric boiler, a heat-sensitive element, a drive motor, a table with holders, and individual adjustment elements. All elements of the shaker are mounted on a steel frame. The metal case is just a fence. The movement of the table of the shaker is carried out elliptically, on which are installed

Erlenmeyer flasks. The range of thermoregulation is 30-100 ° С [Instruction manual for the shaker with a water bath type 357-Lubava, Poland, 1987. - 8 p.].

However, the disadvantage of this installation is the constant control of the water level during long-term operation and the small capacity of the chamber for the flasks. In addition, it is necessary to constantly carry out the disinfection of the device in between the shaking of the media in the flasks.

Thus, the technical task of the proposed utility model is the creation of such an installation that would allow the cultivation of microorganisms in laboratory conditions with stirring and thermostating of the liquid in the vessels.

The technical result of the utility model consists in the duration and effectiveness of the operation of the installation with the simultaneous use of mixing to improve aeration of the bacterial culture and nutrient medium and thermostating in laboratory conditions, with the possibility of disinfection of the heat chamber of the installation during the interval between the cultivation of microorganisms.

The specified technical result is achieved by the fact that the installation for mixing and temperature control of the liquid in the flasks and bottles, containing a heat chamber, a table with holders for flasks, an electric heating element, an electric motor, a control unit, according to a utility model, a heat chamber is suspended from a cable to the ceiling or laboratory cabinet and connected to the control unit, while the thermal chamber consists of an outer and inner chambers, between which a heat insulator is placed, and the capacity of the working cavity of the inner chamber is increased due to the location of holders for flasks, bottles on removable tables and ventilation openings with shutters in the lid and on the bottom of the heat chamber, a disinfecting element is installed on the side of the inner chamber of the inner chamber, in its upper part there is a temperature sensor, in the lower part it is fixed under the table with the holders are an electric heating element, and the electric motor is located in the lower part outside the heat chamber with the possibility of its inclusion only

after closing the lid of the heat chamber, and the amplitude and frequency of oscillations of the heat chamber is possible by adjusting the eccentric on the motor shaft, in addition, a temperature controller and an autotransformer are installed on the control unit.

The new distinctive features of the proposed utility model-installation for mixing and temperature control of liquids in flasks and bottles are:

- suspension of the heat chamber with a cable to the ceiling or laboratory cabinet and connection to an external control unit ensures uniform mixing of culture media in flasks and bottles in laboratory conditions;

- the heat chamber consists of the outer and inner chambers, between which a heat insulator is placed, and the capacity of the working cavity of the inner chamber is increased due to the special arrangement of holders for flasks, bottles on the table and ventilation holes with shutters in the lid and on the bottom of the heat chamber, which ensures a constant temperature environment;

- the use of a disinfecting element inside the working cavity of the inner chamber, in the upper part of the temperature sensor, in the lower part of the electric heating element fixed under the table with holders, provide automatic temperature control within 20-100 ° C and quickly, effectively disinfect the inner chamber with prolonged temperature control;

- the location of the electric motor outside the lower part of the heat chamber with the possibility of turning it on only after closing the lid of the heat chamber provides mechanical vibrations to the heat chamber for shaking liquid samples in flasks, bottles at a constant temperature;

- regulation of the eccentric on the motor shaft provides the necessary amplitude and frequency of oscillations of the thermal chamber;

- the use of a temperature controller and an autotransformer on a remote control unit ensures constant temperature in the capacity of the working cavity of the heat chamber, power to the electric motor and less chance of tilting.

In known devices - analogues, the cultivation of microorganisms is carried out:

1) only due to mechanical stirring of the medium with the help of screws [Copyright certificate No. 371271 of 02/07/89], shaking the table with flasks using an electromechanical drive [Prospectus for the rules for the operation of shaking equipment. - Moscow, 1973 - S.10-11] or 2) the simultaneous use of mixing and maintaining the required temperature in the vessels [Instructions for maintenance of the shaker with a water bath type 357-Lyubava, Poland, 1987. - 8p.]. In this case, in the first cases, plants for growing microorganisms to maintain temperature are placed in special boxes and use mixing devices, driven by a motor. In the second case, the plant for growing microorganisms uses the heat of a water jacket. However, these installations are cumbersome, technically complex and do not use a constant temperature for the cultivation of microorganisms in the cultivation process. A shaker with a water bath requires constant monitoring of the water level during long-term operation in scientific research and a small volume of accommodation of the heat chamber for flasks. In addition, it is necessary to constantly carry out the disinfection of the device in between the shaking of the media in the flasks.

The proposed installation for mixing and temperature control of liquid in flasks and bottles, made in the form of a heat chamber with a table for flasks and bottles, suspended on a cable, connected to an electric motor with an eccentric on the shaft, connected to a control unit to automatically maintain the temperature from 20-100 ° С and to regulate the amplitude and frequency of mixing, it allows you to grow cultures of microorganisms with temperature control, improve mixing and aeration of the medium, to carry out disinfection

heat chamber, use the installation to obtain experimental data in laboratory conditions and in practical health care.

Comparison of the proposed device with other well-known technical solutions from the prior art in patent and scientific documentation made it possible to establish that the authors have not identified solutions that include a combination of features similar and equivalent to those stated. This allows us to conclude that the proposal meets the eligibility criteria of utility models.

The essence of the proposed utility model is illustrated by drawings, in which Fig. 1 shows a unit for mixing and temperature control of liquid in flasks and bottles, Fig. 2 is a view of a removable table with holders from above inside the heat chamber in Fig. 3 is a removable table with holders, installed in bottom of the heat chamber.

The proposed installation for mixing and temperature control of the liquid in the flasks and bottles contains a heat chamber 1, inside of which removable tables 2 with holders 3 for flasks and bottles of various diameters are installed (see figures 1, 2, 3). The thermal chamber 1 is suspended on a cable 4 through an anchor bolt 5 to the ceiling or to the laboratory cabinet (see Fig. 1) and connected to an external control unit 6. The thermal chamber 1 consists of an outer 7 and an inner 8 chambers, between which a heat insulator 9 is placed, representing a laying from a corrugated cardboard. The capacity of the working cavity of the inner chamber 8 is increased due to the special arrangement of holders 3 for flasks, bottles on removable tables 2 (see figure 2): four holders 3 at the corners are perpendicular to the walls of the inner chamber 8, and the rest are at an acute angle and ventilation holes 10 with shutters, respectively, in the lid 12 and on the bottom of the heat chamber 1. Inside the working cavity of the inner chamber 8, a disinfecting element 13 is installed on the side, which is used as an arc mercury lamp DRC 120 for fast and efficient infection with prolonged thermostating. In the upper part of the inner chamber 8, a temperature sensor 14 is installed, and in the lower part there is an electric heating element 15, which

fixed under the table 2 with holders 3 by means of mounting bolts 16. The electric motor 17 is located and attached in the lower part outside the heat chamber 1 with the possibility of turning it on only after closing the cover 12 of the heat chamber 1 when the end switch 18 is mounted inside the cover 12. The amplitude and the oscillation frequency of the heat chamber 1 can be controlled by an eccentric: an adjusting counterweight 19 on the flywheel 20 of the shaft of the electric motor 17 and the autotransformer 21 mounted on the control unit 6. Autotransformer 21, itayuschy motor 17 provides a smooth regulation of the frequency of its revolutions - frequency vibration shaker, practically independent of the load. A temperature controller 22 is installed on the remote control unit b for automatic regulation within 20 - 100 ° C inside the capacity of the working cavity of the inner chamber 8. The installation is turned on using the main switch 23 on the control unit 6.

The proposed installation for mixing and temperature control of the liquid in the flasks and bottles works as follows.

Installation for mixing and temperature control of the liquid in the flasks and bottles is attached using a cable 4 through an anchor bolt 5 to the ceiling or to the laboratory cabinet (Figure 1). Cable 4 adjusts the required height of the installation. Parts of the unit in contact with the objects under study: the inner surface of the chamber 8 of the heat chamber 1, the outer surface 7 is wiped with a swab dipped in 35% hydrogen peroxide solution with the addition of 0.5% detergent (Lotus type) or a swab dipped in 1% chloramine solution. After the parts and surfaces 7, 8 of the installation have dried, after the disinfection works, the disinfecting element includes an arc mercury lamp 13 DRC 120 for 3-5 minutes. The culture medium is placed in flasks or bottles, which are then fixed in the holders 3 of the removable table 2 (Fig.1, 2, 3). Close the lid 12 of the heat chamber 1 until the limit switch 18 trips (a click is heard). Temperature controller 22 on the remote control unit 6

the required constant temperature of cultivation is selected in the range of 20-100 ° C.

The handle of the autotransformer 21 on the remote control unit 6, as well as the eccentric-adjusting counterweight 19 on the flywheel 20 of the motor shaft 17, sets the amplitude and frequency of oscillation of the thermal chamber 1. The installation is turned on using the main switch 23 located on the remote control unit b (Figure 1) .

After shaking and temperature control, the flasks or bottles are removed from the heat chamber 1 when disconnected from the mains and the lid 12 of the heat chamber 1 is opened. The disinfecting element of the mercury arc lamp 13 (DRC 120) is turned on for 3-5 minutes. Then again fix the culture medium in new flasks or bottles in the holders 3 of the table 2 and close the lid 12 of the heat chamber 1 until the operation of the limit switch 18.

The proposed installation for mixing and temperature control of the liquid in the flasks allows, in comparison with known devices in this field, to carry out the cultivation of media with microorganisms while using stirring and constant aeration of them at the required temperature in the heat chamber 1. Simultaneous use of the ventilation system and disinfection of the heat chamber reduces the number of communications and increase the efficiency of maintaining the temperature regime of the media. All this leads to the duration and efficiency of the installation.

Claims (1)

Installation for mixing and temperature control of liquid in flasks and bottles, containing a heat chamber, a table with holders for flasks, an electric heating element, an electric motor, a control unit, characterized in that the heat chamber is suspended from a cable to the ceiling or laboratory cabinet and connected to a remote control unit, the thermal chamber consists of an outer and inner chambers, between which a heat insulator is placed, and the capacity of the working cavity of the inner chamber is increased due to the location of holders for flasks, a bottle On the removable tables and ventilation openings with shutters in the lid and on the bottom of the heat chamber, inside the working chamber of the inner chamber, a disinfecting element is installed on the side, a temperature sensor in the upper part, and an electric heating element fixed under the table with holders, the electric motor being located in the lower part outside the heat chamber with the possibility of its inclusion only after closing the lid of the heat chamber, and the amplitude and frequency of oscillations of the heat chamber is regulated the eccentric shaft of the motor, in addition to the temperature control unit mounted regulator and an autotransformer.