RU2805149C1 - Hepatoprotective tea for treatment of liver diseases - Google Patents

Abstract

FIELD: pharmaceutical industry; hepatoprotective tea.

SUBSTANCE: hepatoprotective tea contains flowers of calendula officinalis, herb of common yarrow, flowers of immortelle sandy, corn columns with stigmas, peppermint leaves, herb of greater celandine, fruits of rose hips, leaves of silver birch, flowers of chamomile and herb of tripartite series with a mass ratio of components, respectively, of 2: 2:2:2:2:1:2:2:2:2. The tea described above expands the arsenal of agents with hepatoprotective and antioxidant effects.

EFFECT: hepatoprotective tea showed comparable effectiveness in comparison with traditional tablet therapy.

1 cl, 1 dwg, 1 tbl, 2 ex

Description

The invention relates to medicine, namely to pharmacy and pharmacognosy, and can be used to treat patients suffering from liver diseases: toxic liver damage; conditions after acute hepatitis; chronic hepatitis of non-viral etiology; liver steatosis (alcoholic and non-alcoholic); cirrhosis of the liver; prevention of liver damage due to prolonged use of medications, alcohol, and chronic intoxication.

Currently, there is an increase in the incidence of liver diseases throughout the world. According to the World Health Organization, more than 2 billion people suffer from liver disease, which is almost 100 times the prevalence of HIV infection. Hepatitis is the most common liver pathology [World Health Organization [Electronic resource]. - URL: http://www.who.int.ra - Cap. from the screen.], therefore, the search for new drugs that have a hepatoprotective effect is relevant. The use of herbal remedies allows one to achieve good results in the treatment of pathology of the hepatobiliary system [Shulpekova, Yu.O. Herbal preparations in the treatment of liver diseases / Yu.O. Shulpekova // Russian Medical Journal. - 2006. - No. 4. - P. 337]. In this regard, the search for new drugs with hepatoprotective effects is extremely important. Certain successes achieved in the treatment of liver diseases are largely associated with the introduction into practice of effective medicines, in particular of herbal origin.

There are known drugs for stabilizing liver cell membranes, normalizing metabolic processes and improving antioxidant activity in organ cells with liver diseases. These include preparations based on the fruit extract of milk thistle, the active substance of which is the flavonoid silymarin under the trade names “Karsil” and “Legalon”. It is known that the hepatoprotective effect is associated with the competitive interaction of silymarin with toxins for the corresponding receptors on the hepatocyte membrane; This is due to the membrane-stabilizing effect. It affects metabolism and other cellular processes by suppressing the 5-lipoxygenase pathway (especially leukotriene B4), binding to free reactive oxygen radicals. Accelerates regenerative processes by stimulating the synthesis of proteins (structural and functional) and phospholipids in affected hepatocytes. The general condition of patients improves, the number of complaints related to the gastrointestinal tract decreases. In patients with reduced digestion associated with liver disease, appetite increases and weight gain occurs. "Karsil" is standardized for silymarin, the content of which is not less than 35 mg [Register of Medicines of Russia RLS. Encyclopedia of drugs. - 21st issue. / Ch. ed. G.L. Vyshkovsky. - M.: LIBROPHARM, 2012].

For the treatment of liver diseases, hepatoprotective collection No. 1 is used: sandy immortelle herb (1 part), knotweed (1 tsp), milk thistle herb (1 tsp), agrimony herb (1 tsp), common cuff herb (1 tsp). ), cinnamon rose hips (1 tsp.) Pour a tablespoon of the mixture with 1 cup of boiling water, leave for 3-6 hours in a thermos, strain. Drink 1/3-1/2 cup three times a day 20-30 minutes before meals [Ivanchenko V.A. Natural medicine. M., 2004, p. 242]. However, this collection does not have a pronounced therapeutic effect due to an insufficient hepatoprotective effect, and with long-term use, side effects of the milk thistle herb on the activity of the heart are possible.

A known herbal collection "Hepatofite" for preparing an infusion, containing a mixture of medicinal plant materials: bean fruit leaves 12 g, dandelion roots 12 g, immortelle flowers 12 g, rose hips 12 g, columns with corn silks 12 g, calendula flowers 11 g , galega officinalis herb 10 g, nettle leaves 10 g, milk thistle fruits 10 g. The collection is used for diseases of the liver and biliary tract. Hepatofite promotes the restoration of liver cells (hepatocytes), improves the antitoxic function of the liver, increases bile secretion, reduces the tone of the gallbladder and bile ducts, increases the concentration of bile acids and reduces the concentration of cholesterol, moderately helps reduce blood sugar levels [Nikolaev S.M., Sambueva Z. .G., Razuvaeva Ya.G., Zandanov A.O., Markhaeva L.E. Hepatoprotective effect of "Hepatofit" in experimental drug-induced hepatitis. Antibiotics and Chemotherapy. 2009; 54 (11-12): 21-24]. The disadvantages of this collection are the limited use in people with low blood sugar levels (risk of hypoglycemia), due to the presence of the herb Galega officinalis and beans, and the low severity of anti-inflammatory and antispasmodic effects.

The closest analogue of the invention is a remedy for the treatment of chronic hepatitis with fibrosis, which is a herbal collection including tansy flowers, yarrow grass, elecampane root, immortelle flowers, corn silk, sweet clover, peppermint leaves, calendula flowers, lingonberry leaves , rhizome with calamus roots, licorice root, dandelion root, bearberry leaves, eucalyptus leaves, wormwood grass, juniper fruits, flax seed, pink periwinkle herb, black elderberry flowers, Scots pine buds, linden flowers, stinging nettle leaves, taken in a certain ratio [patent RU 2164802, 2001]. This collection has antispasmodic, diuretic, pronounced anti-inflammatory and choleretic effects. The disadvantages of this collection are the excessive multicomponents (22 plants), which increases the risk of allergic reactions and the low intensity of the antioxidant effect.

The objective of the invention is to expand the arsenal of drugs that have hepatoprotective, anti-inflammatory, antioxidant effects in order to improve the quality of life of patients.

The technical result is the production of a new oral medicinal product in the form of a collection of medicinal plants that has hepatoprotective, anti-inflammatory, and antioxidant effects.

The proposed hepatoprotective collection for the treatment of liver diseases contains flowers of calendula officinalis, flowers of immortelle sandy, corn columns with stigmas, wild rose hips, great celandine grass, silver birch leaves, peppermint leaves, chamomile flowers, yarrow herb and herb of the trifid series at their mass ratio, respectively, 2:2:2:2:1:2:2:2:2:2.

The proposed collection has an antioxidant effect, is capable of inactivating lipid peroxidation products and restoring cellular metabolism in the liver due to the group of flavonoids predominant in the collection (hyperoside and quercetin) and vitamin C. Essential oils, chelidonin, saponins and flavonoids provide choleretic, antispasmodic, anti-inflammatory, bactericidal and diuretic effects.

All components of the hepatoprotective collection are approved for use in medicine.

The proposed fee is obtained and used as follows. Each type of medicinal plant material that meets the requirements of the State Pharmacopoeia of the XIV edition is crushed individually to a particle size of no more than 3-4 mm. Then the plant material is weighed and thoroughly mixed in equal proportions until a uniform mixture is obtained, the dust is sifted out through a sieve with a hole diameter of 0.18 mm. To obtain an infusion from the collection, 2 tablespoons of the collection are poured into 0.5 liters of water. Leave in a boiling water bath for 15 minutes and then leave at room temperature for 45 minutes, then filter. The infusion is taken 1/2 cup 3 times a day 15-20 minutes before meals for 10 days.

Example 1. The proposed hepatoprotective collection was used in the treatment of 15 patients with diffuse liver damage. All patients, while using the hepatoprotective collection, noted its pleasant taste, mild laxative, diuretic effect, sedative and analgesic effects. The collection was prescribed as an infusion for 7 days. The study involved (with informed consent) 15 patients (7 men, 8 women) with diffuse liver diseases treated at the Republican Center for Surgical Hepatology on the basis of the Russian Clinical Hospital named after. G.G. Kuvatova. The patients were treated for obstructive jaundice of various origins (n=11), or secondary toxic hepatitis (n=4). Patients received an infusion from the collection 2-3 days after surgical elimination of the cause, which led to secondary diffuse liver damage. In the control group (also 15 patients), patients received traditional treatment in the postoperative period.

Clinical patients, when taking the hepatoprotective collection, noted a mild laxative, diuretic effect, as well as a sedative effect. In addition, patients noted the analgesic effect of the collection, which was expressed in the abolition of narcotic analgesics 2.8±0.4 days after surgery (in the control group - abolition of analgesics after 3.7±0.5 days). Based on the results of biochemical liver tests in patients, the following results were obtained, presented in the table.

Based on these results, the hepatoprotective collection showed comparable effectiveness in comparison with traditional tablet therapy.

Example 2. Antioxidant activity of aqueous, alcohol (40% ethyl alcohol) extracts, as well as polyextract obtained from a hepatoprotective collection by fractional maceration using purified water as extractants in a ratio of 1:14, ethyl alcohol 40% - 1:18, alcohol ethyl 70% - 1:14, degree of grinding of raw material particles 1 mm [Baimukhametov I.R. Research on the development of a polyextract from a hepatoprotective collection / Baimukhametov I.R., Kudashkina N.V. // Collection of materials of the VII All-Russian scientific conference of students and graduate students with international participation “Young pharmacy - the potential of the future.” - St. Petersburg: Publishing house SPHFA, 04/24-25/2017. - P. 499-500], determined spectrophotometrically and assessed by the ability to inhibit autoxylation of adrenaline (in vitro) and thereby prevent the formation of reactive oxygen species [Sirota, T.V. Method for determining the antioxidant activity of superoxide mutase and chemical compounds / T.V. Orphan. - Application No. 99103192 (003673), priority dated February 24, 1999]. A 0.05% alcohol solution of quercetin was used as a comparison drug.

The index of antioxidant activity was calculated using the formula:

Where:

AOA - indicator of antioxidant activity, %;

OD1 - change in optical density in the absence of the extract;

OD2 - change in the optical density of the sample in the presence of the extract.

The figure shows a graph of the effect of the studied drugs on the oxidation of adrenaline over time. The graph shows that the infusion, alcohol extract, as well as the polyextract from the studied collection have an effect on the autoxidation of adrenaline, reducing it.

Antioxidant activity, according to the calculations obtained, was 30.5±0.7% for the infusion, 10.1±0.3% for the alcoholic extract, 29.5±1.1% for the polyextract and 32.4±0.9% for comparator drug (quercetin), respectively. According to the methodology used, a value above 10% indicates that the samples under study have antioxidant activity.

Claims (1)

A hepatoprotective collection containing calendula officinalis flowers, yarrow herb, sandy immortelle flowers, corn columns with stigmas, peppermint leaves, characterized in that it additionally contains greater celandine herb, May rose hips, silver birch leaves, chamomile flowers and tripartite herb with a mass ratio of components respectively 2:2:2:2:2:1:2:2:2:2.