RU2800322C2 - Device for determining the effectiveness of caries prophylactic means based on the evaluation of ion transport through a biological membrane - Google Patents

Abstract

FIELD: preventive dentistry.

SUBSTANCE: invention can be used for scientific research and in certification centers to evaluate the effectiveness of caries prophylactic agents. A device for determining the effectiveness of caries prophylactic agents based on the assessment of ion transport through a biological membrane, contains a stand, a holder, a model of tooth enamel in the form of a biological membrane, a thermostat and a glass with a lid and electrodes. The electrodes are made to be connected to a 9 V current source and installed in the lid for interaction with a caries prophylactic agent in the form of a gel and with saline. The holder is installed in a glass and is made for pouring the studied caries prophylactic agent. The biological membrane is made in the form of a reservoir for pouring saline. The thermostat is built into the lid and is designed to maintain the temperature of the studied gel 36.6°C.

EFFECT: determination of the effectiveness of caries prophylactic agents based on the assessment of ion transport through a biological membrane.

1 cl, 1 dwg

Description

Caries is a common disease and over 98 percent of the population has carious lesions. Caries treatment is an expensive and painful process. At the same time, caries prevention is an affordable and safe way to avoid serious complications. The selection of means for the prevention of caries is a complex process that should be based on objective indicators of their composition. The proposed device can solve this problem.

The invention relates to medicine, namely preventive dentistry, and can be used for scientific research, laboratory testing of prophylactic agents in certification centers and other scientific institutions in order to evaluate the effectiveness of various caries prophylactic agents.

At the present stage of development of dentistry, to determine the effectiveness of prophylactic agents, a “Method for determining the remineralizing effectiveness of dental preparations” was proposed by the authors G.G. Ivanova and V.K. Leontiev (SU 1755160 A1 dated 12/19/1992). This method involves conducting clinical trials with patients. A selected group of patients are assigned different options for remineralization therapy using the study agents. The frequency of therapy is prescribed and oral hygiene is taught. Further, various indicators are analyzed (hygiene indices, saliva pH level, RMA index assessment, KOSRE-test and TER-test, electrical conductivity of dental tissues) and follow-up examinations are scheduled after 3 months, after 6 months and after a year. Each appointment involves re-diagnosis according to the above parameters. Further, conclusions are drawn about the results of the effectiveness of caries prophylactic agents.

This method is lengthy, and also involves the participation of patients in the study, which is a significant drawback. The method also does not take into account the natural resistance to caries of tooth enamel, which creates an error in determining the effectiveness of caries prophylactic agents.

The device will be able to determine the effectiveness of caries prophylactic agents based on the analysis of ion transport through a biological membrane - a chicken egg shell, similar in structure to human tooth enamel, which excludes research on living people and allows for an objective assessment. There are no direct analogues of devices for evaluating the effectiveness of funds.

A device for determining the effectiveness of caries preventive agents based on the assessment of ion transport through a biological membrane, containing a tripod, a holder, a model of tooth enamel in the form of a biological membrane, a thermostat and a glass with a lid and electrodes made for connection to a 9-volt current source and installed in the lid for interaction with a caries preventive agent in the form of a gel and with saline, moreover, the holder is installed in a glass and is made for pouring the studied caries preventive agent, and the biological membrane a is made in the form of a reservoir for pouring saline, while the thermostat is built into the lid and is designed to maintain the temperature of the gel under study at 36.6°C.

The design of the device is fully presented in Figure 1. The numbers indicate the components of this device, namely: the glass (1) is installed with a holder (2) and a chicken egg shell (3), after which the test gel is poured into the glass (1), and saline solution is poured into the shell (3). A cover (6) and electrodes (9) are installed on the glass, after which voltage is applied to them (voltage source 9V (10)). With the help of the heater (7) and through the thermostat (8) the required temperature (36.6 degrees Celsius) of the studied gel is maintained.

There is no direct analogue. However, we found a significant drawback - the inability to automatically study the internal solution after passing an electric current. In the future, it is planned to level this shortcoming.

The problem to which the present invention is directed is to develop a device that will be able to evaluate the effectiveness of a caries preventive agent without the participation of people in the experiment based on the assessment of ion transport through a biological membrane simulating human tooth enamel (chicken egg shell).

EFFECT: determination of the effectiveness of caries preventive agents based on the assessment of ion transport through a biological membrane. The specified technical result is achieved by the fact that the pH is determined, and the content of calcium, fluorine and phosphate ions (mmol/l) in the internal solution is determined. Determination of pH allows you to testify to the creation of the necessary alkaline environment for effective enamel remineralization. The quantitative content of ions in the internal solution makes it possible to judge the effectiveness of remineralization, the penetrating ability of ions, and also allows you to ideally select a caries prophylactic gel for a patient, taking into account his clinical situation.

The essential distinguishing features of the proposed technical solution are that:

- people do not participate in the experiment;

- the shell of a chicken egg is used, modeling the enamel of a human tooth;

- the study time is reduced to 5 minutes;

- a device for evaluating the effectiveness of prophylactic remineralizing agents is innovative, unparalleled and reflects several laboratory indicators in the internal solution that characterize the effectiveness of the substance,

- the device allows you to determine not only the concentration of ions in the gel, but also to evaluate the efficiency of ion transport through the tooth enamel, which is important in a comprehensive assessment of the caries prevention composition.

Clinical examples

Example 1 Prophylactic #1 was used as external solution. The experiment was carried out according to the method described above. As a result, the internal solution was investigated (solution pH, active concentration of calcium and phosphate ions). The following results were obtained: pH 6.4 w.e., content of calcium and phosphate ions in solution 0 mmol/l. This tool will not be effective in remineralization, because phosphate ions will not pass through the biological membrane, and they are important elements of the crystal lattice, which ensures the resistance of tooth enamel to caries.

Example 2 Prophylactic #2 was used as external solution. The experiment was carried out according to the method described above. As a result, the internal solution was investigated (solution pH, active concentration of calcium and phosphate ions). The following results were obtained: pH 8.2 w.e., content of calcium ions 0.013 mmol/l, phosphate 0.11 mmol/l. This tool will provide a highly alkaline environment on the surface of tooth enamel and an optimal concentration of ions, which indicates a high remineralizing potential of the gel.

Example 3 Prophylactic #3 was used as external solution. The experiment was carried out according to the method described above. As a result, the internal solution was investigated (solution pH, active concentration of calcium and phosphate ions). The following results were obtained: pH 8.9 w.e., content of calcium ions 0.21 mmol/l, phosphate 0.32 mmol/l. This tool will provide a highly alkaline environment on the surface of tooth enamel and an optimal concentration of ions, which indicates a high remineralizing potential of the gel.

Conclusion. Thus, the use of the device will help to evaluate the effectiveness of caries prevention gels based on ion transport and thus help in clinical medicine, as well as in industry, to create new innovative preventive agents.

Claims (1)

A device for determining the effectiveness of caries prophylactic agents based on the assessment of ion transport through a biological membrane, containing a tripod, a holder, a model of tooth enamel in the form of a biological membrane, a thermostat and a glass with a lid and electrodes made for connection to a 9 V current source and installed in the lid for interacting with a caries prophylactic agent in the form of a gel and with saline, wherein the holder is installed in a glass and is made to in the form of a reservoir for pouring saline, while the thermostat is built into the lid and is designed to maintain the temperature of the gel under study at 36.6°C.

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