RU2796834C1 - Preimplantation method of martine-bell syndrome genetic testing - Google Patents
Preimplantation method of martine-bell syndrome genetic testing Download PDFInfo
- Publication number
- RU2796834C1 RU2796834C1 RU2022129270A RU2022129270A RU2796834C1 RU 2796834 C1 RU2796834 C1 RU 2796834C1 RU 2022129270 A RU2022129270 A RU 2022129270A RU 2022129270 A RU2022129270 A RU 2022129270A RU 2796834 C1 RU2796834 C1 RU 2796834C1
- Authority
- RU
- Russia
- Prior art keywords
- insdseq
- insdqualifier
- insdfeature
- name
- value
- Prior art date
Links
Abstract
Description
Изобретение относится к преимплантационному генетическому тестированию моногенных заболеваний. В настоящее время в мире насчитывается более 350 миллионов людей, страдающих редким заболеванием (по данным RARE Project). Общее количество таких заболеваний по подсчетам European Organization for Rare Diseases (EURORDIS) варьируется от 5 до 7 тысяч. При этом около 80% редких заболеваний имеют генетическую причину. Известная генетическая основа заболевания позволяет с высокой точностью предсказать не только здоровье уже родившегося ребенка, но и оценить риск рождения такого ребенка при анализе генотипов родителей, а также провести генетическую диагностику на самых ранних этапах. Преимплантационное генетическое тестирование (ПГТ) моногенного заболевания становится мощным инструментом для профилактики таких заболеваний.The invention relates to preimplantation genetic testing of monogenic diseases. Currently, there are more than 350 million people in the world suffering from a rare disease (according to the RARE Project). The total number of such diseases, according to the estimates of the European Organization for Rare Diseases (EURORDIS), varies from 5 to 7 thousand. At the same time, about 80% of rare diseases have a genetic cause. The known genetic basis of the disease makes it possible to predict with high accuracy not only the health of an already born child, but also to assess the risk of having such a child by analyzing the genotypes of the parents, as well as to conduct genetic diagnostics at the earliest stages. Preimplantation genetic testing (PGT) of a monogenic disease is becoming a powerful tool for the prevention of such diseases.
Настоящее изобретение относится к способу преимплантационного генетического тестирования синдрома Мартина-Белл. Синдром Мартина-Белл - это наследственное Х-сцепленное заболевание, проявляющееся преимущественно у лиц мужского пола, и являющееся второй, после синдрома Дауна, наиболее частой генетической причиной нарушения умственного развития. Синдром Мартина-Белл встречается с частотой около 1 на 4000 для мужского пола и 1 на 8000 для женского пола. Это заболевание приводит к серьезному нарушению психомоторного развития ребенка, умственной отсталости. Для данного заболевания, при Х-сцепленном доминантном типе наследования, вероятность рождения ребенка мужского пола с этим заболеванием в семье составляет 50%; для детей женского пола вероятность наследования также 50%, но симптомы заболевания выражены намного меньше.The present invention relates to a method for preimplantation genetic testing of Martin-Bell syndrome. Martin-Bell syndrome is a hereditary X-linked disease that occurs predominantly in males, and is the second most common genetic cause of mental retardation after Down syndrome. Martin-Bell syndrome occurs in about 1 in 4000 for males and 1 in 8000 for females. This disease leads to a serious violation of the psychomotor development of the child, mental retardation. For this disease, with an X-linked dominant type of inheritance, the probability of having a male child with this disease in the family is 50%; for female children, the probability of inheritance is also 50%, but the symptoms of the disease are much less pronounced.
К заболеванию синдром Мартина-Белл могут приводить патогенные генетические варианты в гене FMR1, располагающемся на хромосоме X [Garber, K. et. al. Eur J Hum Genet 16, 666-672 (2008)]. Этот ген кодирует белок FMRP (fragile X mental retardation protein, белок умственной отсталости, связанной с синдромом хрупкой X хромосомы), который является РНК-связывающим белком и играет важную роль в развитии синапсов между нейронами, а так же не до конца ясную на данный момент роль в нормальном функционировании женской репродуктивной системы.Martin-Bell syndrome can be caused by pathogenic genetic variants in the FMR1 gene located on chromosome X [Garber, K. et. al. Eur J Hum Genet 16, 666-672 (2008)]. This gene encodes the FMRP protein (fragile X mental retardation protein, mental retardation protein associated with fragile X chromosome syndrome), which is an RNA-binding protein and plays an important role in the development of synapses between neurons, as well as not completely clear at the moment role in the normal functioning of the female reproductive system.
ПГТ синдрома Мартина-Белл проводится для семей, имеющих подтвержденную молекулярно-генетическую природу заболевания. Важно отметить, что обоснование патогенности и каузативности генетических вариантов происходит до проведения ПГТ моногенного заболевания и не входит ни в цели и задачи ПГТ моногенного заболевания, ни в комплекс мероприятий по проведению ПГТ моногенного заболевания. Оценку патогенности проводят по международному стандарту - по критериям, описанным в 2015 году Американским Колледжем Медицинской генетики и Геномики (American College of Medical Genetics and Genomics-Association for Molecular Pathology (ACMG-AMP)) в ходе поиска молекулярно-генетической причины заболевания. ПГТ рекомендуется семьям с высоким риском рождения ребенка с тяжелым (неизлечимым) наследственным заболеванием с установленным патогенными вариантом, обуславливающим этот риск. ПГТ позволяет выбрать из всех эмбрионов, полученных при ЭКО (экстракорпоральном оплодотворении), эмбрионы без патогенного варианта и, следовательно, без риска развития заболевания.PGT of Martin-Bell syndrome is carried out for families with a confirmed molecular genetic nature of the disease. It is important to note that the substantiation of the pathogenicity and causation of genetic variants occurs before the PGT of a monogenic disease and is not included either in the goals and objectives of the PGT of a monogenic disease, or in the complex of measures for conducting PGT of a monogenic disease. Pathogenicity is assessed according to the international standard - according to the criteria described in 2015 by the American College of Medical Genetics and Genomics-Association for Molecular Pathology (ACMG-AMP) in the search for the molecular genetic cause of the disease. PGT is recommended for families at high risk of having a child with a severe (incurable) hereditary disease with an established pathogenic variant that causes this risk. PGT allows you to choose from all embryos obtained by IVF (in vitro fertilization) embryos without a pathogenic variant and, therefore, without the risk of developing a disease.
Основной проблемой при генетической диагностике эмбрионов является -малое исходное количество биоматериала, так как биоптат содержит от одной до трех клеток. В этом случае для повышения эффективности и точности анализа важно как полностью исключить возможность контаминации, так и нивелировать возможный эффект неравномерной и/или неполной амплификации, а также деградации биоматериала. Это требует разработки тест-системы с особыми характеристиками. При этом тест-система разрабатывается с учетом возможности использовать биоматериал разного типа - тотальную дезоксирибонуклеиновую кислоту (ДНК), выделенную из разных тканей, продукт полногеномной амплификации (Whole Genome Amplification, WGA), а также единичные клетки. Сочетание универсальности по отношению к биоматериалу с поэтапной амплификацией целевых фрагментов позволяет проводить анализ нескольких патогенных вариантов для одного образца, в том числе на единичных клетках, а также выявить ситуацию неполной амплификации, контаминации или деградации, образца. Еще одной особенностью ПГТ является отсутствие информации о биологических особенностях эмбриона: в отличие от взрослого человека, у эмбриона могут быть любые хромосомные аномалии, которые усложняют задачу оценки статуса эмбриона по конкретному генетическому варианту. Поэтому тест-система для ПГТ моногенного заболевания должна давать возможность выявить такие случаи и оценить их влияние на достоверность результата диагностики.The main problem in the genetic diagnosis of embryos is the small initial amount of biomaterial, since the biopsy contains from one to three cells. In this case, to improve the efficiency and accuracy of the analysis, it is important both to completely exclude the possibility of contamination and to neutralize the possible effect of uneven and/or incomplete amplification, as well as degradation of the biomaterial. This requires the development of a test system with special characteristics. At the same time, the test system is being developed taking into account the possibility of using different types of biomaterial - total deoxyribonucleic acid (DNA) isolated from different tissues, the product of Whole Genome Amplification (WGA), as well as single cells. The combination of versatility in relation to the biomaterial with step-by-step amplification of target fragments makes it possible to analyze several pathogenic variants for a single sample, including on single cells, as well as to identify the situation of incomplete amplification, contamination or degradation of the sample. Another feature of PGT is the lack of information about the biological characteristics of the embryo: unlike an adult, an embryo can have any chromosomal abnormalities that complicate the task of assessing the status of an embryo for a specific genetic variant. Therefore, a test system for PGT of a monogenic disease should make it possible to identify such cases and evaluate their impact on the reliability of the diagnostic result.
Наиболее близким техническим решением является проведение ПГТ синдрома Мартина-Белл, предложенное Emmanuelle Kieffer и коллегами [Emmanuelle Kieffer et al, 2015] для детекции экспансии CGG-повторов в гене FMR1 с помощью мультплексной гнездовой ПЦР в 2 раунда на единичных клетках с применением косвенной диагностики с помощью анализа 9-ти сцепленных полиморфных маркеров. Учитывая высокую вероятность непрохождения амплификации для прямой диагностики, или низкой информативности прямой диагностики, такая тест-система не позволяет достичь высокой информативности и универсальности. Важным преимуществом предлагаемого нами подхода является использование большего количества полиморфных маркеров для косвенной диагностики, что снижает вероятность недостоверного результата из-за выпадения аллеля или рекомбинации.The closest technical solution is the PGT of Martin-Bell syndrome proposed by Emmanuelle Kieffer and colleagues [Emmanuelle Kieffer et al, 2015] to detect the expansion of CGG repeats in the FMR1 gene using 2-round multiplex nested PCR on single cells using indirect diagnostics with using the analysis of 9 linked polymorphic markers. Taking into account the high probability of failure of amplification for direct diagnostics, or the low information content of direct diagnostics, such a test system does not allow achieving high information content and universality. An important advantage of our approach is the use of a larger number of polymorphic markers for indirect diagnosis, which reduces the likelihood of an unreliable result due to allele dropout or recombination.
Представленный нами метод ПГТ синдрома Мартина-Белл решает задачу разработки более точного способа преимплант ационного генетического тестирования этого моногенного заболевания без использования дорогостоящих приборов и реагентов, который можно было бы применять на биоматериале различного типа: ДНК, выделенную из разных тканей, продукт полногеномной амплификации (WGA), единичные клетки.The method of PGT of Martin-Bell syndrome presented by us solves the problem of developing a more accurate method for preimplantation genetic testing of this monogenic disease without the use of expensive devices and reagents, which could be used on various types of biomaterial: DNA isolated from different tissues, a product of whole genome amplification (WGA ), single cells.
Техническим результатом стало создание тест-системы для диагностики патогенного варианта - экспансии тринуклеотидного повтора CGG в 5'-нетранслируемой области гена FMR1 [Garber, K. et. al. Eur J Hum Genet 16, 666-672 (2008)] с двойной системой детекции - прямой и косвенной. Чаще всего такая мутация наследуется ребенком от матери - носителя премутации, т.е. более короткой экспансии тринуклеотидного повтора в 5'-нетранслируемой области гена, длины которой недостаточно для развития синдрома Мартина-Белл. При формировании яйцеклеток экспансия удлиняется, и ребенок наследует полную мутацию. Такая система необходима при работе с малым количеством биоматериала, так как нестабильная амплификация может привести к потере информации или сниженной точности анализа. Прямая диагностика подразумевает анализ непосредственно наличия-отсутствия патогенного варианта. В данном случае для генетического варианта экспансия тринуклеотидного повтора в 5'-нетранслируемой области гена FMR1 были подобраны праймеры, которые позволяют произвести детекцию нормального аллеля с помощью ПЦР с последующим фрагментным анализом. Косвенная диагностика заключается в анализе наследования молекулярно-генетических маркеров, сцепленных с мутацией, т.е. наследуемых вместе с ней. Для этого на расстоянии не более 3 мБ (что соответствует 3% кроссинговера в среднем) от гена.FMR1 в каждую сторону были выбраны полиморфные локусы, называемые STR (short tandem repeat - короткий тандемный повтор), с гетерозиготностью не менее 0,70 для обеспечения максимальной информативности косвенной диагностики. STR представляют собой повторы 2х и более нуклеотидов расположенные друг за другом (например пара аденин-цитозин (АЦ), повторяющаяся несколько раз подряд: АЦАЦАЦАЦ) и в большом количестве присутствуют в геноме человека. Количество повторов в каждом из них может, варьироваться от индивидуума к индивидууму, а также может быть разным у одного и того же человека на 2х гомологичных хромосомах. Гетерозиготность выше 0,70 означает, что высока вероятность того, что у одного и того же человека количество повторов нуклеотидов в данном STR на одной хромосоме будет отличаться от количества повторов в этом же STR на гомологичной хромосоме, другими словами, аллели данного маркера у этого человека будут отличаться между собой по длине. При амплификации фрагмента, содержащего такой маркер, будут получены ампликоны двух разных длин. Проанализировав количество повторов в нескольких маркерах, окружающих патогенный вариант, и изучив то, как они наследуются в тестируемой семье, можно установить сцепление между аллелями маркеров и патогенным вариантом. Диагностическая ценность исследования количества повторов в данных маркерах у эмбрионов состоит в том, что по тому, какой аллель каждого из маркеров был унаследован эмбрионом, можно судить о том, унаследовал ли эмбрион ген FMR1, несущий патогенный вариант, или же он унаследовал ген FMR1 с другой, гомологичной хромосомы, не содержащий патогенный вариант. Для каждого из этих локусов были подобраны праймеры для амплификации по типу гнездовой или полугнездовой ПЦР в 2 раунда, позволяющей повысить точность и эффективность амплификации. В тест-систему были включены 16 STR локусов для гена FMR1: D17S1449, D17S1452, D17S1455, D17S1460.1, D17S1460.2, D17S1461, D17S1463, D17S1467, D17S1472, D17S1475, FMR1-CA525D, D17S1476, FMR1-GT624D, D17S1477, D17S1482, D17S1483. Праймеры для амплификации находятся на X хромосоме в районе координат 144913360-148380426 (в соответствии с hg19). Последовательности праймеров указаны в формуле изобретения в перечне SEQ ID NO 1-50. Важно отметить, что при подборе праймеров соблюдали ряд особенных требований: длина продукта с внешними праймерами для первого раунда ПЦР не должна превышать 500 п. н. (для наработки с фрагментов, получаемых при полногеномной амплификации), длина продукта с внутренних праймеров для второго раунда ПЦР от 120 до 350 п. н., высокая специфичность внешних праймеров, температура отжига не отличается более, чем на 1°С.The technical result was the creation of a test system for diagnosing a pathogenic variant - the expansion of the CGG trinucleotide repeat in the 5'-untranslated region of the FMR1 gene [Garber, K. et. al. Eur J Hum Genet 16, 666-672 (2008)] with a dual detection system - direct and indirect. Most often, such a mutation is inherited by the child from the mother - the carrier of the premutation, i.e. a shorter expansion of the trinucleotide repeat in the 5'-untranslated region of the gene, which is not long enough for the development of Martin-Bell syndrome. When the eggs are formed, the expansion lengthens, and the child inherits the full mutation. Such a system is necessary when working with a small amount of biomaterial, since unstable amplification can lead to loss of information or reduced accuracy of the analysis. Direct diagnosis involves the analysis of the direct presence-absence of a pathogenic variant. In this case, for the genetic variant of the expansion of the trinucleotide repeat in the 5'-untranslated region of the FMR1 gene, primers were selected that make it possible to detect the normal allele using PCR followed by fragment analysis. Indirect diagnosis consists in the analysis of the inheritance of molecular genetic markers linked to a mutation, i.e. inherited with it. To do this, at a distance of no more than 3 mB (which corresponds to 3% crossover on average) from the FMR1 gene, polymorphic loci called STR (short tandem repeat - short tandem repeat) were selected in each direction with a heterozygosity of at least 0.70 to ensure maximum information content of indirect diagnostics. STRs are repeats of 2 or more nucleotides located one after another (for example, an adenine-cytosine (AC) pair repeated several times in a row: ACACACACA) and are present in large numbers in the human genome. The number of repeats in each of them may vary from individual to individual, and may also be different in the same person on 2 homologous chromosomes. Heterozygosity above 0.70 means that there is a high probability that in the same person the number of nucleotide repeats in this STR on one chromosome will differ from the number of repeats in the same STR on the homologous chromosome, in other words, the alleles of this marker in this person will differ in length. When amplifying a fragment containing such a marker, amplicons of two different lengths will be obtained. By analyzing the number of repeats in several markers surrounding the pathogenic variant and examining how they are inherited in the tested family, linkage between the alleles of the markers and the pathogenic variant can be established. The diagnostic value of studying the number of repeats in these markers in embryos is that by which allele of each of the markers was inherited by the embryo, it can be judged whether the embryo inherited the FMR1 gene carrying the pathogenic variant, or whether it inherited the FMR1 gene from another , a homologous chromosome that does not contain a pathogenic variant. For each of these loci, primers were selected for amplification by the type of nested or semi-nested PCR in 2 rounds, which makes it possible to increase the accuracy and efficiency of amplification. The test system included 16 STR loci for the FMR1 gene: D17S1449, D17S1452, D17S1455, D17S1460.1, D17S1460.2, D17S1461, D17S1463, D17S1467, D17S1472, D17S1475, FMR1- CA525D, D17S1476, FMR1-GT624D, D17S1477, D17S1482 , D17S1483. Primers for amplification are located on the X chromosome in the region of coordinates 144913360-148380426 (according to hg19). The primer sequences are indicated in the claims in the list of SEQ ID NO 1-50. It is important to note that during the selection of primers, a number of special requirements were observed: the length of the product with external primers for the first round of PCR should not exceed 500 bp. (for production from fragments obtained by whole genome amplification), product length from internal primers for the second round of PCR from 120 to 350 bp, high specificity of external primers, annealing temperature does not differ by more than 1°C.
Подготовительный этап ПГТPreparatory stage of the PGT
На подготовительном этапе проводится отработка тест-системы: подбор условий амплификации, оптимальных для работы праймеров, анализ эффективности и специфичности ПЦР-амплификации в обоих раундах, оценки универсальности тест-системы для биообразцов различного типа (ДНК, продукт WGA, единичные клетки). При отработке тест-системы были приготовлены стоковые разведения праймеров с концентрацией 100 mМ, и рабочие разведения комбинаций праймеров (комбинация пар праймеров для 1 и 2 раунда ПЦР) с концентрацией 10 mМ каждого праймера в растворе. Так как в рамках диагностики клинического материала могут быть использованы различные типы матриц, при отработке тест-системы были использованы две биопсии единичных клеток, находящихся в специальном лизирующем буфере (1×PCR Buffer, 0,1% Tween-20, 0,1% Triton X-100, 1 мкг Proteinase K), два образца продуктов полногеномной амплификации биопсиий эмбриона (WGA), а также тотальной ДНК членов семьи, выделенной из крови, для составления родословной и выявления сцепления патогенного варианта с аллелями полиморфных маркеров.At the preparatory stage, the test system is being developed: selection of amplification conditions that are optimal for primers to work, analysis of the efficiency and specificity of PCR amplification in both rounds, assessment of the universality of the test system for biosamples of various types (DNA, WGA product, single cells). When testing the test system, stock dilutions of primers were prepared with a concentration of 100 mM, and working dilutions of primer combinations (a combination of primer pairs for rounds 1 and 2 of PCR) with a concentration of 10 mM of each primer in solution. Since various types of matrices can be used as part of the diagnostics of clinical material, two biopsies of single cells were used in the development of the test system, which were in a special lysis buffer (1 × PCR Buffer, 0.1% Tween-20, 0.1% Triton X-100, 1 µg Proteinase K), two samples of the products of whole genome amplification of embryonic biopsies (WGA), as well as total DNA of family members isolated from blood, for compiling a pedigree and identifying the linkage of a pathogenic variant with alleles of polymorphic markers.
В рамках гнездовой и полугнездовой ПЦР амплификация проводится в два этапа. На первом этапе проводится мультиплексная ПЦР со всеми внешними праймерами для всех локусов, входящих в тест-систему, для обогащения образца всеми целевыми фрагментами. На втором этапе проводится индивидуальная амплификация каждого фрагмента с внутренними праймерами.In nested and semi-nested PCR, amplification is carried out in two steps. At the first stage, multiplex PCR is performed with all external primers for all loci included in the test system to enrich the sample with all target fragments. At the second stage, individual amplification of each fragment with internal primers is carried out.
Полугнездовая ПЦРSemi-nested PCR
Для первого этапа были подобраны внешние высокоспецифичные праймеры для амплификации фрагментов от 300 до 500 п. н. Для второго этапа были, подобраны праймеры для амплификации фрагментов длиной не более 350 пар оснований, а также были введены метки для детекции методом фрагментного анализа. Последовательности праймеров для амплификации фрагментов, содержащих STR локусы, указаны в формуле изобретения в перечне SEQ ID NO 1-50. ПЦР-смесь для первого раунда амплификации содержала 1×ПЦР буфер с Mg2+ (Евроген, Россия), 0.1 mМ каждого деоксинуклеотида, 0.15 μМ каждого праймера, 2,5 U/μl ДНК полимеразы HsTaq (Евроген, Россия), 6% DMSO и 1 мкл тотальной ДНК или 2,5 мкл WGA или 5 мкл лизирующего буфера с образцом в качестве матрицы. Первый этап амплификации проводился по следующему протоколу: этап денатурации 94°С в течение 2 минут, 30 циклов с понижением температуры отжига праймеров с 62 до 45°С в каждом, этап достройки всех матриц 72°С 10 минут. Далее продукты 1-ого этапа были разнесены в индивидуальные пробирки с одной парой праймеров на определенный локус.For the first stage, external highly specific primers were selected to amplify fragments from 300 to 500 bp. For the second stage, primers were selected for the amplification of fragments no longer than 350 base pairs, and labels were introduced for detection by fragment analysis. The sequences of primers for amplification of fragments containing STR loci are indicated in the claims in the list of SEQ ID NO 1-50. The PCR mixture for the first round of amplification contained 1×PCR buffer with Mg2+ (Evrogen, Russia), 0.1 mM of each deoxynucleotide, 0.15 μM of each primer, 2.5 U/μl of HsTaq DNA polymerase (Evrogen, Russia), 6% DMSO, and 1 µl total DNA or 2.5 µl WGA or 5 µl lysis buffer with sample as template. The first stage of amplification was carried out according to the following protocol: the stage of denaturation at 94°C for 2 minutes, 30 cycles with a decrease in the annealing temperature of the primers from 62 to 45°C in each, the stage of completion of all templates at 72°C for 10 minutes. Next, the products of the 1st stage were separated into individual tubes with one pair of primers per specific locus.
В состав ПЦР смеси для второго этапа входили 1×ПЦР буфер с Mg2+ (Евроген, Россия), 0.5xRediLoad™ загрузочный буфер (Thermo Fisher Scientific, USA), 0.2 mM каждого деоксинуклеотида, 0.2 μM каждого праймера, 1U/μl ДНК полимеразы HsTaq (Евроген, Россия), 6% диметилсульфоксида (DMSO) и 1 μl ПЦР-продукта первого этапа амплификации в качестве матрицы. Второй этап амплификации проводился по следующему протоколу: этап денатурации 95°С в течение 2 минут, 35 циклов: денатурация 95°С 30 секунд, отжиг праймеров - 57°С 30 секунд, синтез матрицы - 72°С 1 минута, этап достройки всех матриц 72°С 5 минут. Оценку эффективности и специфичности амплификации проводили с помощью электрофореза в 2% агарозном геле. Результат электрофореза в агарозном геле позволяет определить необходимую степень разведения продуктов амплификации для нанесения на фрагментный анализ (продукты амплификации ДНК членов семьи).The PCR mixture for the second stage included 1×PCR buffer with Mg2+ (Evrogen, Russia), 0.5xRediLoad™ loading buffer (Thermo Fisher Scientific, USA), 0.2 mM of each deoxynucleotide, 0.2 μM of each primer, 1U/μl of HsTaq DNA polymerase ( Evrogen, Russia), 6% dimethyl sulfoxide (DMSO) and 1 μl of the PCR product of the first stage of amplification as a template. The second stage of amplification was carried out according to the following protocol: denaturation stage at 95°C for 2 minutes, 35 cycles: denaturation at 95°C for 30 seconds, primer annealing at 57°C for 30 seconds, template synthesis at 72°C for 1 minute, completion of all templates 72°C 5 minutes. Evaluation of the efficiency and specificity of amplification was performed using electrophoresis in 2% agarose gel. The result of electrophoresis in agarose gel allows you to determine the required degree of dilution of the amplification products for application to fragment analysis (amplification products of DNA of family members).
Фрагментный анализ продуктов амплификации проводили с помощью капиллярного электрофореза на приборе 3130x1 Genetic Analyzer (Applied Biosystems, USA). По результатам фрагментного анализа составляется родословная и отмечаются информативные полиморфные STR-локусы для каждой семьи, которые в дальнейшем будут использованы в клинической диагностике. Локусы делятся на неинформативыне (носитель патогенного варианта гомозиготен по этому локусу), полуинформативные (на некоторых и родительских хромосом аллели по этому маркеру совпадают), информативные (на всех хромосомах родителей аллели этого маркера разные, что дает возможность отличить каждую из них при анализе генотипа эмбриона). Детекция патогенного варианта экспансия тринуклеотидного повтора в 5'-нетранслируемой области гена FMR1 проводилась с помощью праймеров для амплификации: 5'-(6-FAM)-TCAGGCGCTCAGCTCCGTTTCGGTTTCA-3' (F), 5'-AAGCGCCATTGGAGCCCCGCACTTCC-3' (R).Fragment analysis of amplification products was performed using capillary electrophoresis on a 3130x1 Genetic Analyzer (Applied Biosystems, USA). Based on the results of the fragment analysis, a pedigree is compiled and informative polymorphic STR loci for each family are noted, which will later be used in clinical diagnostics. Loci are divided into non-informative (the carrier of the pathogenic variant is homozygous for this locus), semi-informative (alleles for this marker are the same on some and parental chromosomes), informative (alleles of this marker are different on all chromosomes of the parents, which makes it possible to distinguish each of them when analyzing the genotype of the embryo ). Detection of the pathogenic variant expansion of the trinucleotide repeat in the 5'-untranslated region of the FMR1 gene was carried out using amplification primers: 5'-(6-FAM)-TCAGGCGCTCAGCTCCGTTTCGGTTTCA-3' (F), 5'-AAGCGCCATTGGAGCCCCGCACTTCC-3' (R).
Пример 1Example 1
Пациенты APatients A
В ЦГРМ Генетико обратилась семья А, в которой родился ребенок с заболеванием синдром Мартина-Белл с патогенным вариантом экспансия тринуклеотидного повтора CGG в 5'-нетранслируемой области гена FMR1, унаследованном от матери-носителя премутации. Паре было рекомендовано проведение ПГТ заболевания синдром Мартина-Белл в рамках ЭКО для отбора эмбрионов, не унаследовавших заболевание.Family A turned to the CGRM Genetico, in which a child with Martin-Bell syndrome was born with a pathogenic variant of the expansion of the CGG trinucleotide repeat in the 5'-untranslated region of the FMR1 gene inherited from the mother-carrier of the premutation. The couple was recommended to undergo PGT for the disease Martin-Bell syndrome as part of IVF to select embryos that did not inherit the disease.
Гаплотипирование семьиFamily haplotyping
На первом этапе был получен биоматериал (периферическая кровь) членов семьи для детекции патогенного варианта и выявления групп сцепления аллелей полиморфных маркеров. Фрагменты ДНК, содержащие полиморфные маркеры, амплифицировались с помощью праймеров, последовательности которых указаны в формуле изобретения в перечне SEQ ID NO 1-50. Было проанализировано 16 STR локусов. Из них 7 оказались информативными по матери. Таким образом, образцы эмбрионов тестировались только на информативные маркеры.At the first stage, biomaterial (peripheral blood) of family members was obtained to detect the pathogenic variant and identify linkage groups of alleles of polymorphic markers. DNA fragments containing polymorphic markers were amplified using primers, the sequences of which are indicated in the claims in the list of SEQ ID NO 1-50. 16 STR loci were analyzed. Of these, 7 were informative on the mother. Thus, embryo samples were tested only for informative markers.
Аллели полиморфных маркеров, совпадающие у пациентки (матери) и у ребенка с заболеванием синдром Мартина-Белл с заранее установленным носительством патогенного варианта экспансия тринуклеотидного повтора в 5'-нетранслируемой области гена FMR1, признаются сцепленными с патогенным вариантом и друг с другом. Аллели полиморфных маркеров, не совпадающие у матери и у ребенка с заболеванием, признаются сцепленными друг с другом и с нормальным аллелем гена FMR1. Аллели полиморфных маркеров партнера (отца), признаются сцепленными друг с другом и с нормальным геном FMR1. Косвенная диагностика позволяет сделать вывод о наследовании нормального или мутантного аллеля гена даже в случае выпадения аллеля или непрохождения реакции при амплификации фрагментов ДНК для прямой диагностики.Alleles of polymorphic markers that match in a patient (mother) and in a child with a disease of Martin-Bell syndrome with a pre-established carriage of a pathogenic variant, expansion of a trinucleotide repeat in the 5'-untranslated region of the FMR1 gene, are recognized as linked to the pathogenic variant and to each other. Alleles of polymorphic markers that do not match in the mother and in the child with the disease are considered linked to each other and to the normal allele of the FMR1 gene. Alleles of polymorphic markers of the partner (father) are recognized as linked to each other and to the normal FMR1 gene. Indirect diagnostics makes it possible to draw a conclusion about the inheritance of a normal or mutant allele of a gene even if the allele is lost or the reaction fails during amplification of DNA fragments for direct diagnostics.
В результате гаплотипирования и определения групп сцепления для семьи А из примера были получены результаты, представленные в таблице 1. Аллели, указанные на одной строке, располагаются на одной хромосоме, то есть, представляют группу сцепления. Таким образом для пациентки представлено 2 группы сцепления, соответствующие каждой из двух X хромосом. Партнер и ребенок с заболеванием мужского пола имеют только по 1 X хромосоме и только по 1 группе сцепления. N в таблице обозначает отсутствие экспансии тринуклеотидного повтора в 5'-нетранслируемой области гена FMR1, mut -присутствие экспансии тринуклеотидного повтора CGG в 5'-нетранслируемой области гена FMR1, premut - премутацию. Цифрами записаны длины ампликонов в парах нуклеотидов; их длины зависят от количества повторов в маркере STR. Патогенный вариант экспансия тринуклеотидного повтора в 5'-нетранслируемой области гена FMR1 в таблице обозначен коротко - FRAXA.As a result of haplotyping and determination of linkage groups for family A from the example, the results presented in Table 1 were obtained. The alleles indicated on the same line are located on the same chromosome, that is, they represent the linkage group. Thus, the patient has 2 linkage groups corresponding to each of the two X chromosomes. The male partner and child with the disease have only 1 X chromosome and only 1 linkage group. N in the table denotes the absence of trinucleotide repeat expansion in the 5'-untranslated region of the FMR1 gene, mut - the presence of CGG trinucleotide repeat expansion in the 5'-untranslated region of the FMR1 gene, premut - premutation. Numerals indicate the lengths of amplicons in base pairs; their lengths depend on the number of repeats in the STR marker. Pathogenic variant of the expansion of the trinucleotide repeat in the 5'-untranslated region of the FMR1 gene in the table is denoted briefly - FRAXA.
В результате гаплотипирования был сделан вывод, что у пациентки с патогенным вариантом были сцеплены следующие аллели STR-маркеров: DXS1449 - 248, DXS1455 - 222, DXS1460.1 - 236, DXS1463 - 208, GT624D - 249, DXS1482 - 292, DXS1483 - 220.As a result of haplotyping, it was concluded that the following alleles of STR markers were linked in the patient with the pathogenic variant: DXS1449 - 248, DXS1455 - 222, DXS1460.1 - 236, DXS1463 - 208, GT624D - 249, DXS1482 - 292, DXS1483 - 22 0 .
Преимплантационное генетическое тестированиеPreimplantation genetic testing
В цикле ЭКО было получено 6 эмбрионов, проведена биопсия на 5 день развития (в клинике ЭКО), биоптат в буфере для WGA (1xPBS (Invitrogen, США), 1% поливинилпирролидона (PVP) (Fertipro, Бельгия)) направлен в лабораторию «Генетико». Для контроля контаминации на разных этапах работы с образцом в лаборатории разработана система контролей: контроль контаминации буфера для биопсии, контроль контаминации при транспортировке (одна пробирка с буфером не открывается эмбриологом), контроль контаминации каждого образца (проба среды из последней отмывочной капли биопсиийного материала). Все эти контроли вместе с образцами проходят этап полногеномной амплификации, после которого будет заметно малейшее количество ДНК, контаминировавшей контроли. Полногеномную амлификацию проводили с помощью коммерческого набора SurePlex (lllumina, США).In the IVF cycle, 6 embryos were obtained, a biopsy was performed on the 5th day of development (in the IVF clinic), the biopsy in WGA buffer (1xPBS (Invitrogen, USA), 1% polyvinylpyrrolidone (PVP) (Fertipro, Belgium)) was sent to the Genetico ". To control contamination at different stages of working with a sample, the laboratory developed a system of controls: control of contamination of the biopsy buffer, control of contamination during transportation (one test tube with buffer is not opened by the embryologist), control of contamination of each sample (sample of the medium from the last wash drop of biopsy material). All these controls, together with the samples, go through the stage of whole genome amplification, after which the smallest amount of DNA contaminating the controls will be noticeable. Genome-wide amplification was performed using the commercial SurePlex kit (lllumina, USA).
Продукт полногеномной амплификации, а также ДНК всех членов семьи амплифицировали на 1 этапе в мультиплексной ПЦР с праймерами для детекции патогенного варианта и праймерами для информативных для семьи А полиморфных маркеров в соответствии с разработанным в рамках подготовительного этапа протоколом для тест-системы. Также были добавлены праймеры для маркеров половых хромосом - SRY/AMEL. На 2 этапе амплификацию проводили для каждого маркера отдельно в соответствии с разработанным протоколом для тест-системы. Таким образом были установлены группы сцепления, унаследованные каждым эмбрионом. Полученные результаты:The product of genome-wide amplification, as well as the DNA of all family members, was amplified at stage 1 in multiplex PCR with primers for the detection of the pathogenic variant and primers for polymorphic markers informative for the A family in accordance with the protocol for the test system developed as part of the preparatory stage. Primers for sex chromosome markers, SRY/AMEL, were also added. At stage 2, amplification was performed for each marker separately in accordance with the developed protocol for the test system. In this way, the linkage groups inherited by each embryo were established. Results:
По результатам прямой и косвенной диагностики 4 эмбрионов (эмбрионы 3, 4, 5 и 6) не унаследовали заболевание, у 2 эмбрионов (эмбрионы 1 и 2) выявлен гаплотип, соответствующий унаследованному заболеванию. Эмбрионы, не унаследовавшие заболевание, были рекомендованы к переносу по результатам ПГТ-М.According to the results of direct and indirect diagnostics, 4 embryos (embryos 3, 4, 5, and 6) did not inherit the disease; in 2 embryos (embryos 1 and 2), a haplotype corresponding to the inherited disease was detected. Embryos that did not inherit the disease were recommended for transfer based on the results of PGT-M.
--->--->
<?xml version="1.0" encoding="UTF-8"?><?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE ST26SequenceListing PUBLIC "-//WIPO//DTD Sequence Listing <!DOCTYPE ST26SequenceListing PUBLIC "-//WIPO//DTD Sequence Listing
1.3//EN" "ST26SequenceListing_V1_3.dtd">1.3//EN" "ST26SequenceListing_V1_3.dtd">
<ST26SequenceListing dtdVersion="V1_3" fileName="Способ <ST26SequenceListing dtdVersion="V1_3" fileName="Method
преимплантационного генетического тестирования синдрома preimplantation genetic testing syndrome
Мартина-Белл_11111.xml" softwareName="WIPO Sequence" Martina-Bell_11111.xml" softwareName="WIPO Sequence"
softwareVersion="2.2.0" productionDate="2022-12-02">softwareVersion="2.2.0" productionDate="2022-12-02">
<ApplicationIdentification> <ApplicationIdentification>
<IPOfficeCode>RU</IPOfficeCode> <IPOfficeCode>EN</IPOfficeCode>
<ApplicationNumberText>2022129270</ApplicationNumberText> <ApplicationNumberText>2022129270</ApplicationNumberText>
<FilingDate>2022-11-11</FilingDate> <FilingDate>2022-11-11</FilingDate>
</ApplicationIdentification> </ApplicationIdentification>
<ApplicantFileReference>2022129270</ApplicantFileReference> <ApplicantFileReference>2022129270</ApplicantFileReference>
<EarliestPriorityApplicationIdentification> <EarliestPriorityApplicationIdentification>
<IPOfficeCode>RU</IPOfficeCode> <IPOfficeCode>EN</IPOfficeCode>
<ApplicationNumberText>2022129270</ApplicationNumberText> <ApplicationNumberText>2022129270</ApplicationNumberText>
<FilingDate>2022-11-11</FilingDate> <FilingDate>2022-11-11</FilingDate>
</EarliestPriorityApplicationIdentification> </EarliestPriorityApplicationIdentification>
<ApplicantName languageCode="ru">Акционерное общество "Центр <ApplicantName languageCode="en">Joint-Stock Company "Center
Генетики и Репродуктивной Медицины "Генетико" Genetics and Reproductive Medicine "Genetics"
</ApplicantName></ApplicantName>
<ApplicantNameLatin>Genetico</ApplicantNameLatin> <ApplicantNameLatin>Genetico</ApplicantNameLatin>
<InventionTitle languageCode="ru">Способ преимплантационного <InventionTitle languageCode="en">Preimplantation method
генетического тестирования Мартина-Белл</InventionTitle>genetic testing Martin-Bell</InventionTitle>
<SequenceTotalQuantity>50</SequenceTotalQuantity> <SequenceTotalQuantity>50</SequenceTotalQuantity>
<SequenceData sequenceIDNumber="1"> <SequenceData sequenceIDNumber="1">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q2"> <INSDQualifier id="q2">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>caaatccctccctgatctg</INSDSeq_sequence> <INSDSeq_sequence>caaatccctccctgatctg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="2"> <SequenceData sequenceIDNumber="2">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q4"> <INSDQualifier id="q4">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>caagacaatctcaccgctct</INSDSeq_sequence> <INSDSeq_sequence>caagacaatctcaccgctct</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="3"> <SequenceData sequenceIDNumber="3">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q6"> <INSDQualifier id="q6">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gtgaatgaggctctggttc</INSDSeq_sequence> <INSDSeq_sequence>gtgaatgaggctctggttc</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="4"> <SequenceData sequenceIDNumber="4">
<INSDSeq> <INSDSeq>
<INSDSeq_length>24</INSDSeq_length> <INSDSeq_length>24</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..24</INSDFeature_location> <INSDFeature_location>1..24</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q8"> <INSDQualifier id="q8">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>agatcttggctatgtgttactaca</INSDSeq_sequence> <INSDSeq_sequence>agatcttggctatgtgttactaca</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="5"> <SequenceData sequenceIDNumber="5">
<INSDSeq> <INSDSeq>
<INSDSeq_length>23</INSDSeq_length> <INSDSeq_length>23</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..23</INSDFeature_location> <INSDFeature_location>1..23</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q10"> <INSDQualifier id="q10">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ctgtttcattctaagattcaggc</INSDSeq_sequence> <INSDSeq_sequence>ctgtttcattctaagattcaggc</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="6"> <SequenceData sequenceIDNumber="6">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q12"> <INSDQualifier id="q12">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>aatcccccataatcacactct</INSDSeq_sequence> <INSDSeq_sequence>aatcccccataatcacactct</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="7"> <SequenceData sequenceIDNumber="7">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q14"> <INSDQualifier id="q14">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>tcagaacatcttgggcatct</INSDSeq_sequence> <INSDSeq_sequence>tcagaacatcttgggcatct</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="8"> <SequenceData sequenceIDNumber="8">
<INSDSeq> <INSDSeq>
<INSDSeq_length>18</INSDSeq_length> <INSDSeq_length>18</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..18</INSDFeature_location> <INSDFeature_location>1..18</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q16"> <INSDQualifier id="q16">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gctgactaggcatgttcg</INSDSeq_sequence> <INSDSeq_sequence>gctgactaggcatgttcg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="9"> <SequenceData sequenceIDNumber="9">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q18"> <INSDQualifier id="q18">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gaggcatgatgggtaactatac</INSDSeq_sequence> <INSDSeq_sequence>gaggcatgatgggtaactatac</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="10"> <SequenceData sequenceIDNumber="10">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q20"> <INSDQualifier id="q20">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gcatgggataaagcaggta</INSDSeq_sequence> <INSDSeq_sequence>gcatgggataaagcaggta</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="11"> <SequenceData sequenceIDNumber="11">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q22"> <INSDQualifier id="q22">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gggaaaaaggttggtgaga</INSDSeq_sequence> <INSDSeq_sequence>gggaaaaaggttggtgaga</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="12"> <SequenceData sequenceIDNumber="12">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q24"> <INSDQualifier id="q24">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>cccaagcctccacaataat</INSDSeq_sequence> <INSDSeq_sequence>cccaagcctccacaataat</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="13"> <SequenceData sequenceIDNumber="13">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q26"> <INSDQualifier id="q26">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ttactcattacaactgggcaac</INSDSeq_sequence> <INSDSeq_sequence>ttactcattacaactgggcaac</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="14"> <SequenceData sequenceIDNumber="14">
<INSDSeq> <INSDSeq>
<INSDSeq_length>17</INSDSeq_length> <INSDSeq_length>17</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..17</INSDFeature_location> <INSDFeature_location>1..17</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q28"> <INSDQualifier id="q28">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ttcactggtcagcggag</INSDSeq_sequence> <INSDSeq_sequence>ttcactggtcagcggag</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="15"> <SequenceData sequenceIDNumber="15">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q30"> <INSDQualifier id="q30">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>cctctcacaagaagactgca</INSDSeq_sequence> <INSDSeq_sequence>cctctcacaagaagactgca</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="16"> <SequenceData sequenceIDNumber="16">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q32"> <INSDQualifier id="q32">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>acaagcacaatagaagcaggt</INSDSeq_sequence> <INSDSeq_sequence>acaagcacaatagaagcaggt</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="17"> <SequenceData sequenceIDNumber="17">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q34"> <INSDQualifier id="q34">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gggagctgagcattagttc</INSDSeq_sequence> <INSDSeq_sequence>gggagctgagcattagttc</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="18"> <SequenceData sequenceIDNumber="18">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q36"> <INSDQualifier id="q36">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gggttacaacgtactcctgtag</INSDSeq_sequence> <INSDSeq_sequence>gggttacaacgtactcctgtag</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="19"> <SequenceData sequenceIDNumber="19">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q38"> <INSDQualifier id="q38">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gtcatttggaggaaagacact</INSDSeq_sequence> <INSDSeq_sequence>gtcatttggaggaaagacact</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="20"> <SequenceData sequenceIDNumber="20">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q40"> <INSDQualifier id="q40">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>cctgatgacttggcctatg</INSDSeq_sequence> <INSDSeq_sequence>cctgatgacttggcctatg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="21"> <SequenceData sequenceIDNumber="21">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q42"> <INSDQualifier id="q42">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ggttcacttttcttttgagtca</INSDSeq_sequence> <INSDSeq_sequence>ggttcacttttcttttgagtca</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="22"> <SequenceData sequenceIDNumber="22">
<INSDSeq> <INSDSeq>
<INSDSeq_length>24</INSDSeq_length> <INSDSeq_length>24</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..24</INSDFeature_location> <INSDFeature_location>1..24</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q44"> <INSDQualifier id="q44">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>tgtatggtagaatatgctagtgga</INSDSeq_sequence> <INSDSeq_sequence>tgtatggtagaatatgctagtgga</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="23"> <SequenceData sequenceIDNumber="23">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q46"> <INSDQualifier id="q46">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ggtaaaagctgcaacctacaa</INSDSeq_sequence> <INSDSeq_sequence>ggtaaaagctgcaacctacaa</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="24"> <SequenceData sequenceIDNumber="24">
<INSDSeq> <INSDSeq>
<INSDSeq_length>25</INSDSeq_length> <INSDSeq_length>25</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..25</INSDFeature_location> <INSDFeature_location>1..25</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q48"> <INSDQualifier id="q48">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gagtctaattaggttttactgtggc</INSDSeq_sequence> <INSDSeq_sequence>gagtctaattaattaggttttactgtggc</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="25"> <SequenceData sequenceIDNumber="25">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q50"> <INSDQualifier id="q50">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gatcatcggtaggttaggagg</INSDSeq_sequence> <INSDSeq_sequence>gatcatcggtaggttaggagg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="26"> <SequenceData sequenceIDNumber="26">
<INSDSeq> <INSDSeq>
<INSDSeq_length>18</INSDSeq_length> <INSDSeq_length>18</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..18</INSDFeature_location> <INSDFeature_location>1..18</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q52"> <INSDQualifier id="q52">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ctggaaacgctttgatga</INSDSeq_sequence> <INSDSeq_sequence>ctggaaacgctttgatga</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="27"> <SequenceData sequenceIDNumber="27">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q54"> <INSDQualifier id="q54">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>actgggcattaagagtcacat</INSDSeq_sequence> <INSDSeq_sequence>actgggcattaagagtcacat</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="28"> <SequenceData sequenceIDNumber="28">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q56"> <INSDQualifier id="q56">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>aggatggtgtaatagtgggact</INSDSeq_sequence> <INSDSeq_sequence>aggatggtgtaatagtgggact</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="29"> <SequenceData sequenceIDNumber="29">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q58"> <INSDQualifier id="q58">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gaagcccagttcctagagaa</INSDSeq_sequence> <INSDSeq_sequence>gaagcccagttcctagagaa</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="30"> <SequenceData sequenceIDNumber="30">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q60"> <INSDQualifier id="q60">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gtgctgagatttggtcaaga</INSDSeq_sequence> <INSDSeq_sequence>gtgctgagatttggtcaaga</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="31"> <SequenceData sequenceIDNumber="31">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q62"> <INSDQualifier id="q62">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ctgtttccatgcttgctataa</INSDSeq_sequence> <INSDSeq_sequence>ctgtttccatgcttgctataa</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="32"> <SequenceData sequenceIDNumber="32">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q64"> <INSDQualifier id="q64">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ctgataacaaagcacgtgtgt</INSDSeq_sequence> <INSDSeq_sequence>ctgataacaaagcacgtgtgt</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="33"> <SequenceData sequenceIDNumber="33">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q66"> <INSDQualifier id="q66">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gacatgggcatttctaagg</INSDSeq_sequence> <INSDSeq_sequence>gacatgggcatttctaagg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="34"> <SequenceData sequenceIDNumber="34">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>other DNA</INSDQualifier_value> <INSDQualifier_value>other DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q68"> <INSDQualifier id="q68">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ttctccttttcattcttcctg</INSDSeq_sequence> <INSDSeq_sequence>ttctccttttcattcttcctg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="35"> <SequenceData sequenceIDNumber="35">
<INSDSeq> <INSDSeq>
<INSDSeq_length>24</INSDSeq_length> <INSDSeq_length>24</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..24</INSDFeature_location> <INSDFeature_location>1..24</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q70"> <INSDQualifier id="q70">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gcatctctttattctgctacactc</INSDSeq_sequence> <INSDSeq_sequence>gcatctctttattctgctacactc</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="36"> <SequenceData sequenceIDNumber="36">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q72"> <INSDQualifier id="q72">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>tcaccaggtaccatttgtgt</INSDSeq_sequence> <INSDSeq_sequence>tcaccaggtaccatttgtgt</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="37"> <SequenceData sequenceIDNumber="37">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q74"> <INSDQualifier id="q74">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gtgctgcagtgttagttacca</INSDSeq_sequence> <INSDSeq_sequence>gtgctgcagtgttagttacca</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="38"> <SequenceData sequenceIDNumber="38">
<INSDSeq> <INSDSeq>
<INSDSeq_length>18</INSDSeq_length> <INSDSeq_length>18</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..18</INSDFeature_location> <INSDFeature_location>1..18</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q76"> <INSDQualifier id="q76">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ggccaattacaaacccag</INSDSeq_sequence> <INSDSeq_sequence>ggccaattacaaacccag</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="39"> <SequenceData sequenceIDNumber="39">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q78"> <INSDQualifier id="q78">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>atttactgggtactggactgtg</INSDSeq_sequence> <INSDSeq_sequence>atttactgggtactggactgtg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="40"> <SequenceData sequenceIDNumber="40">
<INSDSeq> <INSDSeq>
<INSDSeq_length>21</INSDSeq_length> <INSDSeq_length>21</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..21</INSDFeature_location> <INSDFeature_location>1..21</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q80"> <INSDQualifier id="q80">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>attgaatctgctaggtaggca</INSDSeq_sequence> <INSDSeq_sequence>attgaatctgctaggtaggca</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="41"> <SequenceData sequenceIDNumber="41">
<INSDSeq> <INSDSeq>
<INSDSeq_length>24</INSDSeq_length> <INSDSeq_length>24</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..24</INSDFeature_location> <INSDFeature_location>1..24</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q82"> <INSDQualifier id="q82">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gaaactgagagtctgagaagtctg</INSDSeq_sequence> <INSDSeq_sequence>gaaactgagagtctgagaagtctg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="42"> <SequenceData sequenceIDNumber="42">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q84"> <INSDQualifier id="q84">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>caacaacagagacagccaa</INSDSeq_sequence> <INSDSeq_sequence>caacaacagagacagccaa</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="43"> <SequenceData sequenceIDNumber="43">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q86"> <INSDQualifier id="q86">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>aggaccgaggctcttttag</INSDSeq_sequence> <INSDSeq_sequence>aggaccgaggctcttttag</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="44"> <SequenceData sequenceIDNumber="44">
<INSDSeq> <INSDSeq>
<INSDSeq_length>22</INSDSeq_length> <INSDSeq_length>22</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..22</INSDFeature_location> <INSDFeature_location>1..22</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q88"> <INSDQualifier id="q88">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>caggctctcgttctacttagct</INSDSeq_sequence> <INSDSeq_sequence>caggctctcgttctacttagct</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="45"> <SequenceData sequenceIDNumber="45">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q90"> <INSDQualifier id="q90">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>caagttgaacacgattgca</INSDSeq_sequence> <INSDSeq_sequence>caagttgaacacgattgca</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="46"> <SequenceData sequenceIDNumber="46">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q92"> <INSDQualifier id="q92">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gctaaaagtggcttggatct</INSDSeq_sequence> <INSDSeq_sequence>gctaaaagtggcttggatct</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="47"> <SequenceData sequenceIDNumber="47">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q94"> <INSDQualifier id="q94">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>cgtcagctacaactcaaagg</INSDSeq_sequence> <INSDSeq_sequence>cgtcagctacaactcaaagg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="48"> <SequenceData sequenceIDNumber="48">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q96"> <INSDQualifier id="q96">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>gtgcaagccttagagtgatg</INSDSeq_sequence> <INSDSeq_sequence>gtgcaagccttagagtgatg</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="49"> <SequenceData sequenceIDNumber="49">
<INSDSeq> <INSDSeq>
<INSDSeq_length>19</INSDSeq_length> <INSDSeq_length>19</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..19</INSDFeature_location> <INSDFeature_location>1..19</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q98"> <INSDQualifier id="q98">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>cacaaaagggcttattgga</INSDSeq_sequence> <INSDSeq_sequence>cacaaaagggcttattggga</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
<SequenceData sequenceIDNumber="50"> <SequenceData sequenceIDNumber="50">
<INSDSeq> <INSDSeq>
<INSDSeq_length>20</INSDSeq_length> <INSDSeq_length>20</INSDSeq_length>
<INSDSeq_moltype>DNA</INSDSeq_moltype> <INSDSeq_moltype>DNA</INSDSeq_moltype>
<INSDSeq_division>PAT</INSDSeq_division> <INSDSeq_division>PAT</INSDSeq_division>
<INSDSeq_feature-table> <INSDSeq_feature-table>
<INSDFeature> <INSDFeature>
<INSDFeature_key>source</INSDFeature_key> <INSDFeature_key>source</INSDFeature_key>
<INSDFeature_location>1..20</INSDFeature_location> <INSDFeature_location>1..20</INSDFeature_location>
<INSDFeature_quals> <INSDFeature_quals>
<INSDQualifier> <INSDQualifier>
<INSDQualifier_name>mol_type</INSDQualifier_name> <INSDQualifier_name>mol_type</INSDQualifier_name>
<INSDQualifier_value>unassigned DNA</INSDQualifier_value> <INSDQualifier_value>unassigned DNA</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
<INSDQualifier id="q100"> <INSDQualifier id="q100">
<INSDQualifier_name>organism</INSDQualifier_name> <INSDQualifier_name>organism</INSDQualifier_name>
<INSDQualifier_value>unidentified</INSDQualifier_value> <INSDQualifier_value>unidentified</INSDQualifier_value>
</INSDQualifier> </INSDQualifier>
</INSDFeature_quals> </INSDFeature_quals>
</INSDFeature> </INSDFeature>
</INSDSeq_feature-table> </INSDSeq_feature-table>
<INSDSeq_sequence>ttgtttatcggttgctgtgt</INSDSeq_sequence> <INSDSeq_sequence>ttgtttatcggttgctgtgt</INSDSeq_sequence>
</INSDSeq> </INSDSeq>
</SequenceData> </SequenceData>
</ST26SequenceListing></ST26SequenceListing>
<---<---
Claims (4)
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2796834C1 true RU2796834C1 (en) | 2023-05-29 |
Family
ID=
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2620944C1 (en) * | 2016-12-27 | 2017-05-30 | Федеральное государственное бюджетное научное учреждение "Научный центр неврологии" (ФГБНУ НЦН) | Method for detection of expansion of trinucleotide cgg-repeats in 5'-untranslated, promotor region of fmr1 gene in case of ataxy/ tremor syndrome associated with fragile x-chromosome (fxtas) |
| CN109355376A (en) * | 2018-12-04 | 2019-02-19 | 深圳会众生物技术有限公司 | Fragile X mental retardation FMR1 genetic test primer, kit and detection method |
| US20200399699A1 (en) * | 2019-06-24 | 2020-12-24 | Origin Biotechnology Co., Ltd. | Kits for diagnosing fragile x syndrome and uses thereof |
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2620944C1 (en) * | 2016-12-27 | 2017-05-30 | Федеральное государственное бюджетное научное учреждение "Научный центр неврологии" (ФГБНУ НЦН) | Method for detection of expansion of trinucleotide cgg-repeats in 5'-untranslated, promotor region of fmr1 gene in case of ataxy/ tremor syndrome associated with fragile x-chromosome (fxtas) |
| CN109355376A (en) * | 2018-12-04 | 2019-02-19 | 深圳会众生物技术有限公司 | Fragile X mental retardation FMR1 genetic test primer, kit and detection method |
| US20200399699A1 (en) * | 2019-06-24 | 2020-12-24 | Origin Biotechnology Co., Ltd. | Kits for diagnosing fragile x syndrome and uses thereof |
Non-Patent Citations (1)
| Title |
|---|
| Garber KB, Visootsak J, Warren ST. Fragile X syndrome. Eur J Hum Genet. 2008 Jun;16(6):666-72. doi: 10.1038/ejhg.2008.61. Epub 2008 Apr 9. PMID: 18398441; PMCID: PMC4369150. * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sobenin et al. | Quantitative assessment of heteroplasmy of mitochondrial genome: perspectives in diagnostics and methodological pitfalls | |
| US20080311565A1 (en) | Methods and Kits for Detecting Germ Cell Genomic Instability | |
| US20220228214A1 (en) | Assay for quantitating the extent of methylation of a target site | |
| Valaperta et al. | Identification and characterization of DM1 patients by a new diagnostic certified assay: neuromuscular and cardiac assessments | |
| AU2022211822A1 (en) | Major histocompatibility complex single nucleotide polymorphisms | |
| Capucchio et al. | Degenerative myelopathy in German Shepherd Dog: comparison of two molecular assays for the identification of the SOD1: c. 118G> A mutation | |
| US20100297633A1 (en) | Method of amplifying nucleic acid | |
| US20220372575A1 (en) | A kit for detection of mutations causing genetic disorders | |
| RU2796834C1 (en) | Preimplantation method of martine-bell syndrome genetic testing | |
| KR101677127B1 (en) | Primer set for genotyping of horse | |
| RU2816650C1 (en) | Method for preimplantation genetic testing of smith-lemli-opitz syndrome | |
| RU2795481C1 (en) | Preimplantation genetic testing method for alport syndrome | |
| RU2795482C1 (en) | Preimplantation genetic testing method for achondroplasia | |
| RU2803650C1 (en) | Method of preimplantation genetic testing for spondyloepiphyseal dysplasia | |
| RU2799541C1 (en) | Method of preimplantation genetic testing of hereditary zonular cataract | |
| RU2799538C1 (en) | Preimplantation genetic testing method for blepharophimosis, ptosis and epicanthus reversal syndrome type 1,2 | |
| RU2795483C1 (en) | Method for preimplantation genetic testing of type 4 osteopetrosis | |
| RU2791878C1 (en) | Method for preimplantation genetic testing of non-syndromic sensorineural hearing loss | |
| RU2808833C1 (en) | Method of preimplantation genetic testing of facioscapulohumeral muscular dystrophy | |
| RU2777091C1 (en) | Method for pre-implantation genetic testing of breast and ovarian cancer | |
| RU2777081C1 (en) | Method for pre-implantation genetic testing of louis-bar syndrome | |
| RU2772938C1 (en) | Method for pre-implantation genetic testing of familial hypertrophic cardiomyopathy | |
| RU2795796C1 (en) | Preimplantation genetic testing method for achondroplasia | |
| RU2775416C1 (en) | Method for preimplantation genetic testing of duchenne/becker muscular dystrophy | |
| RU2769300C1 (en) | Test system for pre-implantation genetic testing of type 1 spinocerebellar ataxia |