RU2794784C1 - Fungicidal composition for seed treatment - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to a fungicidal composition against fungal pathogenic organisms for the control of crop diseases. The fungicidal composition for dressing seeds contains tebuconazole, prothioconazole, pyraclostrobin and fludioxanil in the amount of 16–26.6 wt.% in the composition as active ingredients, agriculturally acceptable excipients and water up to 100 wt.%. The ratio of active ingredients is, by weight parts: fludioxonil 4.76–37.59, pyraclostrobin 5.64–71.43, prothioconazole 4.76–56.39, and tebuconazole 0.38–19.05. As agriculturally acceptable excipients, the composition contains at least one dispersant and/or emulsifier, defoamer, antifreeze, wetting agent-adhesive, liquid dye, stabilizer-thickener, bactericide, fatty acid methyl esters or vegetable oil, and, optionally, an organic solvent.

EFFECT: proposed fungicidal composition for seed dressing has a high fungicidal activity, which makes it possible to reduce the environmental burden by minimizing the doses of active substances introduced into the environment while maintaining high plant protection efficiency and reduce the degree of resistance due to the synergistic action of four active substances belonging to different groups of chemical compounds.

9 cl, 2 tbl

Description

The invention relates to the field of fungicides, specifically, to a fungicidal composition against fungal pathogenic organisms containing active compounds from the groups of phenylpyroles, strobilurins and triazoles, which are used in agriculture to combat diseases of crops.

A composition is known containing a strigolactone derivative and a fungicidal compound selected from the group, in particular fludioxonil, in mass ratios of 1:1-1014. The composition may additionally contain a fungicidal compound selected from the group - prothioconazole, tebuconazole or pyraclostrobin. The composition further comprises an agriculturally acceptable substrate, carrier, filler and/or surfactant and is applied via seed treatment, foliar application, stem application, application to seeds, plant or plant fruit by irrigation/immersion (chemigation) or soil application and /or on an inert substrate, pumice, pyroclastic materials/tuff, synthetic organic substrates, organic substrates or liquid substrate, where the plant is grown or should be grown (patent RU 2444194, IPC A01N 43/653, A01N 43/12, A01N 43/08, A01N 43/56, A01N 37/50, A01N 37/46, A01P 3/00, A01P 13/00, 03/10/2012).

The disadvantage of the composition is the presence in its composition of a derivative of strigolactone, which is a plant hormone produced by plant roots, which is difficult to obtain on the market. The complex composition of the composition limits its wide application.

The closest is the fungicidal synergistic combination, contains tebuconazole, prothioconazole and pyraclostrobin in a synergistically effective weight ratio (0.2-5):(0.2-8):1 (patent RU 2763596, IPC A01N 29/04, A01N 31/08 , A01N 37/30, A01N 43/26, A01N 43/50, A01N 43/653, A01P 3/00, 12/30/2021). The fungicidal combination is used as concentrates, as direct spray solutions, suspensions, including also concentrated aqueous or anhydrous suspensions, or as a microemulsion, for seed treatment, spraying, fine spraying and other treatments.

The disadvantage of the composition is the ecological load of the fungicidal composition on the environment.

The objective of the invention is to develop a fungicidal composition for plant protection, specifically for dressing seeds in order to control diseases at an early stage of plant growth.

The technical result is an increase in the biological activity of the fungicidal composition, which makes it possible to reduce the environmental burden by minimizing the doses of active substances introduced into the environment while maintaining the high efficiency of protective treatments and reduce the degree of resistance due to the synergistic action of four active substances belonging to different groups of chemical compounds.

The technical result is achieved by using a fungicidal composition for dressing seeds containing tebuconazole, prothioconazole and pyraclostrobin as active ingredients, while the composition additionally contains fludioxonil, with the content of active substances in the composition of 16-26.6 wt.%, agriculturally acceptable excipients and water up to 100 wt.%, while the ratio of active substances is, wt.h.:

fludioxonil 4.76-37.59 pyraclostrobin 5.64-71.43 prothioconazole 4.76-56.39 tebuconazole 0.38-19.05

The fungicidal composition for seed dressing is characterized in that, as agriculturally acceptable excipients, it contains at least one dispersant and/or emulsifier, defoamer, antifreeze, wetting agent, liquid dye, stabilizer-thickener, bactericide, fatty acid methyl esters or vegetable oil, and optionally an organic solvent.

The fungicidal seed dressing composition is characterized in that it contains 7-14% by weight of a dispersant and/or an emulsifier.

The fungicidal composition for dressing seeds is characterized by the fact that it contains 0.2 wt.% of a defoamer and a stabilizer-thickener.

The fungicidal composition for seed dressing is characterized by the fact that it contains 5 wt.% of antifreeze and liquid dye.

The fungicidal seed dressing composition is characterized in that it contains 4% by weight of a wetting agent.

The fungicidal composition for seed dressing is characterized in that it contains 0.1% by weight of the bactericide.

The fungicidal composition for seed dressing is characterized in that it contains 12-20% by weight of fatty acid methyl esters or 5-20% by weight of vegetable oil.

The fungicidal seed dressing composition is characterized in that it may contain 2-12% by weight of an organic solvent.

The claimed composition of the fungicidal composition for seed treatment is characterized by the presence of four active substances belonging to three classes of chemical compounds, which ensures high biological activity of the composition due to the synergistic action of the active substances and largely avoids the development of resistance of pathogenic organisms to its action. At the same time, a significant amount of vegetable oil and / or a chemical derivative of vegetable oil (FAME) and their possible combinations with an organic solvent not only provide a suspension medium (in combination with water), but also act as an adjuvant, reduce the toxic load on the environment while maintaining high fungicidal efficiency.

As active substances (AI) in the composition of the fungicidal composition, the following are used:

Fludioxonil - supplier Zhechem (China). Belongs to the class of phenylpyrol compounds (analogue of natural antimycotic substances). Broad-spectrum fungicide, inhibits the growth of mycelium, inhibits glucose phosphorylation during cellular respiration.

Pyraclostrobin - supplier of Platform Agrotech Co., Ltd. Belongs to the group of strobilurins. A contact fungicide with a long-term protective effect, capable of blocking the mitochondrial respiration of pathogenic cells.

Prothioconazole - supplier Zhechem (China). Belongs to the class of triazole compounds (a subclass of triazolintions). Systemic fungicide against a wide range of diseases with a long-term therapeutic effect. The fungicide has an immunizing effect.

Tebuconazole - supplier of Astec LifeSciences Ltd. Belongs to the class of triazole compounds. Systemic fungicide. Suppresses the biosynthesis of ergosterol in cell membranes of phytopathogens,

As auxiliary components, the fungicidal composition contains at least one dispersant and / or emulsifier, defoamer, antifreeze, wetting agent-adhesive, liquid dye, stabilizer-thickener, bactericide and, for the preparation of a suspension medium, water and methyl esters of fatty acids (FAME) (manufacturer OJSC "Grodno Azot") or vegetable oils or mixtures thereof with an organic solvent.

A block copolymer of ethylene oxide and propylene oxide, for example, Tensiofix DB08 (manufacturer OmniChem Ind. (import)), can be used as a dispersant and/or emulsifier for preparing the composition; ethoxylated tristyrylphenol Agnique TSP 16 (manufacturer BASF (import)); ethoxypropoxytristyrylphenol Soprpophor TSP/461; Soprophor 796/P (manufacturer Solvay (import)); copolymer of ethylene oxide and propylene oxide Atlox 4894 (manufacturer Croda Europe Ltd. (import)); organic sulfonate Tensiofix SC, (manufacturer Omnichem Ind. (import)); ethoxylated sorbitan trioleate, e.g. Alkamuls T/85-V (manufacturer Solvay (import)), polyglycol Tensiofix 26100 (manufacturer Omnichem Ind. (import)), mixtures of anionic and nonionic surfactants based on oxyalkylated alcohols and calcium salt of alkylbenzenesulfonic acid, e.g. , Berol 9968/9960 (manufacturer AkzoNobel (import)).

Sunflower oil (manufactured by Rusagro Group of Companies LLC), soybean oil (manufactured by Amuragrocenter LLC), castor oil (manufactured by ElGroup Company LLC), rapeseed oil (manufactured by OAO Kosulinskoye, ProFet LLC), and as organic solvents, for example, Solvesso 150 or 200 (manufacturer ExxonMobil), petroleum orthoxylene (manufacturer OAO Bashneft), xylenes, toluene. As an antifreeze, for example, propylene glycol, manufacturer Shandong Shida Shenghua Chemical Group Co., Ltd (import, China) and other glycols (ethylene glycol, diethylene glycol, glycerin), antifoam, for example, Penta 465 (manufacturer Penta-91 LLC) ""), wetting agent - 10 wt.% aqueous solution of polyvinyl alcohol (manufacturer HebeiSmartChemicals Co. Ltd. (import)), thickener, for example, Tenziofix 821 - xanthan gum manufactured by Omnichem Ind. (import), a biocide, for example, Proxel GXL (solution of benzisothiazol-3(2H)-one, manufacturer Arch UK Biocides Ltd (import)), and as a dye - Rhodamine B in the form of a 30 wt.% aqueous solution (manufacturer ALL- CHEMIE LTD (India)).

The fungicidal composition for seed dressing is prepared as follows. An aqueous suspension of fludioxonil is prepared, the resulting suspension is ground in a bead mill in the presence of a defoamer and at least one dispersant. The ground mixture is mixed with a solution of pyraclostrobin, prothioconazole and tebuconazole in vegetable oil or FAME or their mixture with an organic solvent in the presence of at least one emulsifier.

Next, the required amount of liquid dye and other auxiliary components - antifreeze, wetting agent, thickener and biocide are added to the resulting mixture. As a result of mixing, a ready-to-use suspo-emulsion concentrate is obtained.

Examples of the compositions of fungicidal compositions for dressing seeds in the form of suspo-emulsion concentrates are presented in table 1.

The effectiveness of the fungicidal composition for dressing seeds is shown in table 2.

For studies of the effect of the fungicidal composition for dressing seeds, wheat seeds with known low germination and high infestation were used to obtain contrasting results. The laboratory germination of seeds was determined according to GOST 12038-84. - 07/01/86. Seeds of agricultural crops. Germination methods.

Germinated seeds are normally germinated. Normally germinated seeds include seeds that have: well-developed roots (or the main germinal root) that have a healthy appearance; well developed and intact hypocotyl genu (hypocotyl) and supracotyl genu (epicotyl) with normal apical bud; two cotyledons - in dicots; primary leaflets, occupying at least half the length of the coleoptile, are in cereals.

Normally germinated seeds also include seedlings with small defects.

Non-germinated seeds include: swollen seeds that have not germinated by the time of the final germination count, but have a healthy appearance and are not crushed when pressed with tweezers.

Dissimilar seeds include: rotten seeds with soft decomposed endosperm, blackened or rotten embryo and seedlings with partially or completely rotted roots, cotyledons, bud, hypocotyl, epicotyl; abnormally germinated seeds that have one of the following disorders in the development of seedlings: there are no germinal roots or they are less than the established norm, or they are short, stopped growing, weak, spirally twisted, watery; the main germinal root is shortened, swollen, stunted, long filiform, fusiform, has a longitudinal crack or damage affecting the conductive tissues, watery, bifurcated; coleoptile empty, cracked, shorter than leaves, deformed, absent; primary leaflets occupy less than half of the coleoptile or are discolored, shattered or split longitudinally, fusiform, watery, usually with short or stunted germinal roots; the kidney is missing or rotten; the hypocotyl is short and thickened, twisted, curved, watery, segmented, with a constriction or with an open crack affecting the conductive tissues; the epicotyl is short and thickened, twisted, constricted, with an open crack affecting conductive tissues; both cotyledons are lost more than 1/2 or completely, abnormally enlarged with a shortened knee; the cotyledon of the onion without a pronounced “knee” is poorly developed.

Seed infestation was determined according to GOST 12044-93 Seeds of agricultural crops. Methods for determining the incidence of diseases.

In sterile Petri dishes with a diameter of 9.5-10 cm, 10 cm of sterilized agarized Chapek's medium was poured. The thickness of the medium layer in the Petri dish should be 3-4 mm. Pouring nutrient media into cups and laying seeds was carried out in a bacteriological chamber (sterile box). Seeds were laid out on a solidified nutrient medium with tweezers, periodically sterilizing it by burning on an alcohol lamp. The seeds were washed with a stream of water under a tap for 1-2 hours and disinfected with 96% alcohol for 1-2 minutes. Then the seeds were washed in sterile or boiled water and dried between sheets of sterile filter paper. Seeds were treated with mixtures of 1-10, the control was left untreated, then placed in Petri dishes, 10-25 pcs. depending on the culture and put them for germination in a thermostat at a temperature of 22°C - 25°C. Germination of seeds was carried out during the period specified to determine the germination of seeds.

The Fusarium oxysporum strain MFG58518 provided by the Laboratory of Mycology and Phytopathology of the FGBNU VIZR was used. The strain was cultivated in Petri dishes on Czapek's dense nutrient medium. The plates were placed in a box under illumination with a 30 W erythema lamp to induce sporulation. To obtain a static culture of phytopathogenic fungi, 100 µl of a suspension of fungal spores containing 10 ³ CFU was added to sterile flasks with 50 ml of a sterile liquid nutrient medium. Fungicide solutions were added to the same flasks so that their concentration in the medium was from 1.0 to 12.5 ppm.

No fungicides were added to the control flask.

Mycelium was grown for 10-14 days. Then, the culture liquid and mycelium were filtered through pre-dried at 55°C and weighed filters (white or red ribbon), the resulting filters with mycelium were dried at 55°C to constant weight, reweighed, and the mass of mycelium was calculated.

As can be seen from Table 2, the fungicides in the disinfectant had a fungistatic effect - the infection of grain and the growth of F. oxysporum mycelium in the enrichment culture decreased compared to the control.

Thus, the fungicidal composition for dressing seeds containing active ingredients is 0.38-19.05 wt.h. tebuconazole, 4.76-56.39 wt.h. prothioconazole, 5.64-71.43 wt.h. pyraclostrobin and 4.76-37.59 wt.h. fludioxonil in combination with agriculturally acceptable excipients at the content of active ingredients in the composition of 16-26.6 wt.%, provides an increase in the biological activity of the fungicidal composition, which reduces the environmental burden by minimizing the doses of active substances introduced into the environment while maintaining high efficiency of protective treatments and reduce the degree of resistance due to the synergistic action of four active substances belonging to different groups of chemical compounds.

Claims (10)

1. Fungicidal composition for dressing seeds containing tebuconazole, prothioconazole and pyraclostrobin as active ingredients, characterized in that the composition additionally contains fludioxonil, with the content of active substances in the composition of 16-26.6 wt.%, agriculturally acceptable excipients and water up to 100 wt.%, while the ratio of active substances is, wt.h.: fludioxonil 4.76-37.59 pyraclostrobin 5.64-71.43 Prothioconazole 4.76-56.39 Tebuconazole 0.38-19.05 2. Fungicidal composition according to claim 1, characterized in that it contains at least one dispersant and / or emulsifier, defoamer, antifreeze, wetting agent, liquid dye, stabilizer-thickener, bactericide, fatty acid methyl esters as agriculturally acceptable excipients or vegetable oil and optionally an organic solvent. 3. Fungicidal composition according to claim 2, characterized in that it contains 7-14 wt.% of the dispersant and/or emulsifier. 4. Fungicidal composition according to claim 2, characterized in that it contains 0.2 wt.% of a defoamer and a stabilizer-thickener. 5. Fungicidal composition according to claim 2, characterized in that it contains 5 wt.% of antifreeze and liquid dye. 6. Fungicidal composition according to claim 2, characterized in that it contains 4 wt.% wetting agent-adhesive. 7. Fungicidal composition according to claim 2, characterized in that it contains 0.1 wt.% bactericide. 8. Fungicidal composition according to claim 2, characterized in that it contains 12-20 wt.% methyl esters of fatty acids or 5-20 wt.% vegetable oil. 9. Fungicidal composition according to claim 2, characterized in that it may contain 2-12 wt.% organic solvent.