RU2790666C1 - Use of micellized form of curcumin as a feed additive for farm animals - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: use of a food additive containing micellized curcumin as a feed additive for farm animals.

EFFECT: improved feed additive for farm animals.

3 cl, 6 tbl

Description

The invention relates to the field of agriculture.

Curcumin (diferuloylmethane) is an extract from the root of the turmeric plant (lat. Cúrcuma) and belongs to the group of curcuminoids. They also include dimethoxycurcumin and bis-dimethoxycurcumin. Curcumin is commonly used as a food coloring and is also found in dietary supplements.

The positive effect of curcumin on the human body is well studied, its anti-inflammatory and antitumor properties, the ability to fight free radicals, improve brain function and reduce the risk of cardiovascular diseases have been confirmed. At the same time, the use of curcumin is not limited to use only in the nutrition or treatment of people. It is also recommended as an additive in livestock feed to improve animal health and productivity, and reduce the negative effects of indoor crowding. It is also known that more bioavailable forms of curcumin (for example, liposomes and micelles) are widely used in the production of dietary supplements to improve human well-being and health, and research is underway on the use of such forms in pharmaceuticals for the prevention and treatment of various diseases.

However, the natural form of curcumin is poorly soluble in water and has low bioavailability, which affects the effect, while an excessive increase in the dosage of curcumin in the feed is not rational and does not always achieve the desired result. Thus, the problem solved when creating the claimed invention is to develop new digestible products based on curcumin used as feed additives for farm animals, while the technical result achieved in solving this problem is to increase the bioavailability of such products to improve productivity. animals, reducing the level of oxidized lipids in animal meat and increasing the biological value of such products.

To achieve the desired result, it is proposed to use a food additive containing micelle curcumin as a feed additive for farm animals, while this additive contains curcumin in an amount of not more than 10 wt.% and a surfactant with a hydrophilic-lipophilic value. balance (HLB) more than 9.

Preferably, the content of curcumin in the supplement is not more than 7.5 wt.%, and the surfactant can be selected from the group including polysorbate 20, polysorbate 80, polyethylene glycol or its derivatives, sucrose fatty acid esters, or mixtures thereof.

An example of this additive is the complex food additive "ExtraOx^® Curcumin”, EAEU declaration of conformity No. RU D-RU.RA03.V.94286/21, in which the content of micellized curcumin is at least 6 wt.% (but not more than 10 wt.%), and polysorbate 80 is used as a surfactant.

The production process of this additive includes obtaining a micellar solution by introducing curcumin powder into an excess of surfactant of more than 80% with an HLB value of more than 9 at an elevated temperature and further heating with constant stirring. At room temperature, the finished product is a clear viscous liquid that can be diluted with water without becoming cloudy, which is achieved by the formation of micelles that are smaller than the wavelength of light. The particle size is in the range from 5 to 20 nm, which is important for the stability of the micellar solution and increasing bioavailability.

Together with the All-Russian Research and Technological Institute of Poultry of the Russian Academy of Sciences (FNTs "VNITIP" RAS), a study was made of the effect of the micellized form of curcumin according to the present invention on the condition of broiler chickens. The experiment was carried out on two groups of chickens, 35 heads each, without segregation by sex. Broiler chickens of the "Change" cross-breeding from one day to 35 days of age were used. Feeding was carried out with dry full-ration loose compound feed with nutritional value according to the VNITIP 2019 standards without restricting access to food and water. The feed of the control group did not contain curcumin, while the feed of the second experimental group was supplemented with ExtraOx ^® Curcumin at a dosage of 200 g/t. The content of curcuminoids in the ExtraOx ^® Curcumin supplement is 6.5%, thus, taking into account the dosage, 13 g/t of pure curcuminoids were added to the feed. The additive was introduced by its preliminary dissolution in vegetable oil.

Zootechnical results of the study on the effect of the ExtraOx ^® Curcumin additive are shown in Table 1. From the data presented, it can be seen that the productivity of broilers in the group using the ExtraOx ^® Curcumin additive was better than control in all growing periods. Thus, the live weight of chickens at 7, 21 and 35 days of age significantly exceeded the control by 2.0, 7.8 and 7.2%, respectively, while the live weight of males was significantly higher than the control group by 6.2%, and chickens - by 8.4%. The use of the mycelized form of curcumin also had a positive effect on feed conversion and contributed to a decrease in this indicator by 10.7%.

Evaluation of broilers according to the productivity index (EIP) showed that the inclusion of "ExtraOx ^® Curcumin" in the poultry feed made it possible to increase the productivity index of chickens in this group by 70.82 points compared to the control group.

Table 1

Index Group #1 Control #2 ExtraOx® Curcumin Livestock safety, % 100 100 Live weight, g at age, days:
per diem
7
21
35 (average) 42.0±0.34
169.3±2.24
839.11±12.17
1993.3 42.0±0.34
175.80±2.02 (+2.0%)
904.31±14.45 (+7.8%)
2136.74 (+7.2%) including:
roosters
chickens 2175.80±39.40
1810.80±33.28 2311.31±53.79 (+6.2%)
1962.17±38.53 (+8.4%) Feed costs per head, kg 3.071 3.067 Feed costs per 1 kg of live weight gain, kg 1.639 1.464 Average daily live weight gain, g 57.38 61.61 EIP, score 349.98 420.8

Table 2 shows the chemical composition (%) and the content of vitamins and carotenoids (µg/g) in the liver of broiler chickens at the age of 35 days after slaughter. As can be seen from the obtained data, the content of crude protein in the micellar curcumin group is higher by 8.05%, while the fat content decreased by 7.71%, which indicates the absence of a cytotoxic effect on liver cells when using "ExtraOx ^® Curcumin" at the studied dosage.

In addition, the content of vitamin A in the liver of broilers in the experimental group was higher by 32.47% compared to chickens in the control group. A similar pattern was observed for the accumulation of vitamin E in the liver - its level by the end of cultivation in the second group exceeded the control by this indicator by 19.6%, which, in turn, indicates a reduced formation of free radicals in the body of chickens of this group.

table 2

Index Group #1 Control #2 ExtraOx® Curcumin Moisture 65.61 67.79 Crude protein 58.81 66.86 crude fat 23.38 15.67 raw ash 4.05 4.62 Vitamin A 135.23 179.15 Vitamin E 14.71 17.59 Vitamin B ₂ 11.74 11.70 Carotenoids 0.49 0.77

The study of hematological and biochemical parameters in broilers showed that the level of total protein, leukocyte formula, erythrocyte count in chickens of both groups practically did not differ and was within the physiological norm for a given age of chickens.

The addition of a mycelialized form of curcumin also improved meat quality. Tables 3 and 4 show the results of checking the chemical composition of the homogenate of the pectoral and leg muscles of broilers, respectively (% air. dry matter). The content of protein in the pectoral muscles of cockerels and hens increased by 2.33 and 1.45% in comparison with the control, with a decrease in fat content by 1.36% in cockerels and by 0.99% in hens of the experimental group. The content of protein in the leg muscles of cockerels and hens in the experimental group increased by - 2.53 and 8.21%, due to a decrease in fat content - by 4.49% in cockerels and by 11.09% in hens.

Table 3

Index Petushki chickens Group #1 Control #2 ExtraOx® Curcumin #1 Control #2 ExtraOx® Curcumin Moisture 69.50 70.35 73.61 69.25 Crude protein 85.60 87.93 88.23 89.68 crude fat 6.61 5.25 3.60 2.61 raw ash 4.42 4.43 4.40 4.65

Table 4

Index Petushki chickens Group #1 Control #2 ExtraOx® Curcumin #1 Control #2 ExtraOx® Curcumin Moisture 67.47 62.59 64.01 67.24 Crude protein 66.99 69.52 60.83 69.04 crude fat 27.66 23.17 34.21 23.12 raw ash 3.71 3.23 3.28 3.56

Thus, it was confirmed that the use of micellar form of curcumin in accordance with the claimed invention has a positive effect on the intensity of protein, carbohydrate and lipid metabolism. Due to this, broiler chickens gained weight more efficiently without increasing feed intake, which led to a decrease in the cost of poultry meat and an increase in economic efficiency. Also, by increasing the protein in the muscles and reducing the amount of fat, such meat is a more valuable food product for the population. Another benefit is the increased shelf life of meat due to the accumulation of antioxidants and the reduction of free radical formation.

An experiment was also conducted to test the effectiveness of the native non-micelle form of curcumin in growing broiler chickens. In this experiment, the food of the control group also did not contain curcumin, while native curcumin was added to the food of the experimental group. In this case, curcumin was used, mixed with a neutral carrier (silicon dioxide), the content of curcuminoids in it was 6.5%, by analogy with the product "ExtraOx ^® Curcumin". The dosage of the native form in the feed was also 200 g/t or 13 g/t of pure curcuminoids. Curcumin was added by mixing with dry ingredients. Table 5 presents the main zootechnical results.

Table 5

Index Group #1 Control #2 Native form of curcumin Livestock safety, % 100 100 Live weight, g at age, days:
per diem
7
21
36 (average) 39.0±0.34
128.57±2.16
716.0±20.30
1991.05 39.0±0.39
131.98±2.64 (+2.7%)
718.09±12.69 (+0.6%)
1976.09 (-0.75%) including:
roosters
chickens 2146.0±47.32
1836.1±34.77 2067.73±54.74 (-3.6%)
1884.45±32.79 (+2.6%) Feed costs per head, kg 3.230 3.268 Feed costs per 1 kg of live weight gain, kg 1.675 1.699 Average daily live weight gain, g 57.77 55.35 EIP, score 332.96 325.78

The study showed that the native form of curcumin at a dosage similar to "ExtraOx ^® Curcumin" does not have a positive effect on the growth of broiler chickens. The average body weight in the experimental group with curcumin on day 36 was 0.75% less than the weight of chickens in the control group.

During storage in fats, incl. animal origin, oxidation processes occur, which lead to a deterioration in their quality, which leads to product spoilage. Organoleptically, this manifests itself in the appearance of an unpleasant rancid taste and smell, often there is a change in color or texture. To test the effect of the ExtraOx ^® Curcumin feed additive on the subsequent storage of broiler meat, the thiobarbituric number was measured in the samples. This characteristic is an indicator of oxidative deterioration of fats and is often used to control meat and meat products. For this, minced chicken meat was prepared along with the skin from carcasses from each group (separately from rooster and chicken). The obtained samples after cooling were transferred to the laboratory for analysis. The results obtained are presented in table 6.

Table 6

Group Thiobarbituric number, mgMA/kg #1 Control Rooster 0.078±0.008 Chicken 0.156±0.015 #2 ExtraOx® Curcumin Rooster 0.039±0.004 Chicken 0.066±0.006

From the data obtained, it can be seen that in the group of broiler chickens that received "ExtraOx ^® Curcumin", oxidative spoilage is on average 2.2 times lower compared to the control group. Such meat will be better stored and retain good organoleptic properties longer by slowing down the oxidative processes of chicken fat.

Thus, the use of the micellized form of curcumin "ExtraOx ^® Curcumin" in accordance with the claimed invention, on the example of broiler chickens, showed significant results, expressed in improving the health of chickens, improving feed absorption and, as a result, in increasing live weight with a decrease in feed intake, which together led to a decrease in the cost of meat produced and an increase in profitability in the cultivation of poultry. Meat from broiler chickens fed with the ExtraOx ^® Curcumin diet will also retain its properties better during storage and sale. It is also obvious to specialists that the use of micellar curcumin in accordance with the claimed invention is possible when growing not only chickens, but also other farm animals.

Claims (3)

1. The use of a food additive containing curcumin in micellar form in an amount of not more than 10 wt.% and a surface-active substance (surfactant) with a hydrophilic-lipophilic balance (HLB) value of more than 9, as a feed additive for poultry. 2. Use according to claim 1, wherein the content of curcumin in the supplement is not more than 7.5% by weight. 3. Use according to claim 1 or 2, wherein the surfactant is selected from the group consisting of polysorbate 20, polysorbate 80, polyethylene glycol or its derivatives, sucrose fatty acid esters, or mixtures thereof.