RU2763589C1 - Method for producing powder extract from grape snail - Google Patents

Abstract

FIELD: powdered extracts producing.

SUBSTANCE: invention relates to methods for producing powdered extracts from grape snails. The method includes the following stages: preparatory, during which the snail placed in the cage is provided with flour and water for 3-5 days, after which the snail is provided with only water for 5 days, keeping at a temperature of 19-22°С. Then the prepared snail is mechanically removed from the shell and placed in a homogenizer, to which water is added in an amount of 30% of the snails mass. After that, the homogeneous and homogeneous snail mass is dried and then sterilized.

EFFECT: invention improves the efficiency and manufacturability of the process of obtaining the extract, reducing the time and energy consumption when obtaining a powdered extract of high quality.

5 cl, 2 dwg, 1 tbl

Description

The field of technology to which the invention belongs

The invention relates to methods for obtaining powdered extracts from grape snails, which can be used for the preparation of cosmetics (extracts, gels, creams, ointments, pastes), as well as in medicine, pharmacology and in the production of biologically active additives, in the food industry and in others areas of the national economy.

State of the art

Currently, one of the promising areas in medicine and pharmacology is the creation of drugs based on biologically active substances of natural origin. Grape snails (lat. Helix pomatia) have found their application in cosmetology and medicine, where they are used due to the high content of nutrients, vitamins and minerals, including: choline - 13%, vitamin B12 - 16.7%, vitamin E - 33 .3%, potassium - 15.3%, magnesium - 62.5%, phosphorus - 34%, iron - 19.4%, copper - 40%, selenium - 49.8%.

It is known that grape snail extract, containing mucopolysaccharides, enzymes and amino acids, has a beneficial protective and regenerating effect on the gastric and intestinal mucosa, quickly eliminates painful symptoms and dyspepsia, improves appetite and suppresses insomnia. The symptoms are relieved without the use of sedatives (contained in many of the drugs) and the treatment is generally more stable and durable than with currently used drugs and does not have their unpleasant side effects (German patent application DE2318787, IPC classes A61K 35/56, A61K 17/00, published 10/24/1974).

Known methods for obtaining powdered extracts from plant materials: RF patents for inventions: No. 2458114, IPC class C12C 1/18, publ. 08/10/2012; No. 2547710, IPC class A23L 1/10, publ. 04/10/2015; as well as extracts from plant material with anaerobic gram-negative bacteria: RF patent for invention No. 2370532, IPC classes C12N 1/20, A23L 1/105, A61K 35/74, publ. 10/20/2009.

A known method for obtaining an extract with immunomodulating action from animal raw materials (AS USSR for invention No. 1442064, class IPC A61K 37/24, publ. pyrogen-free bidistilled water and high molecular weight components are precipitated in a flow heater for 10 min at 80°C. Then the pulp is cooled and centrifuged. Pyrogen-free water is added to the sediment for continuous ultrafiltration. The ultrafiltrate is subjected to electrodialysis for 2 hours at 30°C, voltage 12V, maximum current 40A. The extract yield is 1.6-1.54%.

A known method of purification of Helix pomatia agglutinin (German application for invention DE 1948610, IPC class C07K 14/435, publ. solution, optional concentration, precipitation (NH ₄ ) 2SO ₄ salt-free dialysis and lyophilization.

The closest in technical essence to the proposed method is a method for the production of an enzyme extract from frozen snails selected from the species Helix aspersa, Helix pomatia, Helix lucorum or combinations thereof (French patent application FR 3014902, IPC classes C12N 9/42, A61K 8/66 , A61P 1/00, C12N 9/24, published 06/19/2015). This method includes the steps:

- preparation of a mixture containing purified water and frozen snails;

- grinding frozen snails in the specified mixture;

- enzymatic extraction in the aqueous phase at room temperature;

- separation of liquid and solid substances;

- spray drying, freeze drying or hot air flow.

The drying step is carried out with a stream of hot air, the temperature of which is 30-45°C. Additionally, the method may contain:

- the stage of grinding said snails before the stage of preparing the mixture or before the stage of grinding;

a concentration step by vacuum evaporation after the liquid-solid separation step and before the drying step;

- a filtration step after the liquid/solid separation step.

The enzymatic extraction step in the aqueous phase is carried out with stirring for 1 hour. The solid/liquid separation step is carried out by centrifugation at about 4000 rpm.

The disadvantages of the known method are the relatively low quality of the obtained extract, high energy costs and the duration of the production cycle, and as a result, the lack of efficiency and manufacturability of the extract production process.

Brief description of the drawings

In FIG. 1-2 are photos of the obtained powdered extract from the grape snail.

Disclosure of invention

The objective of the invention is to develop an easily implemented, scalable technology for obtaining a concentrated powdered extract from a grape snail for use in medicine, pharmacology, or as a food additive (BAA).

The technical result is an increase in the efficiency and manufacturability of the process of obtaining an extract, reducing time and energy costs when obtaining a high quality powdered extract.

The specified technical result is achieved using a method that includes the following steps: preparatory, during which the snail placed in the cage is provided with flour and water for 3-5 days, after which the snail is provided with only water for 5 days, keeping at a temperature of 19-22 °C; then the prepared snail is mechanically removed from the shell and placed in a homogenizer, to which water is added in an amount of 30% by weight of the snails; after which the homogeneous and homogeneous snail mass is dried and further sterilized.

Drying can be carried out in a spray dryer at a temperature of 40-45°C or by lyophilization.

Drying can be carried out within 24-26 hours.

Sterilization can be carried out within 20-30 minutes.

Implementation of the invention

The essence of the proposed method is as follows.

The method for obtaining a powdered extract from a grape snail consists of the following steps:

1. Preparation of a grape snail.

The snail in the cage is fed for 3-5 days with flour, also provided with water, after which they do not feed for 5 days, leaving only water and the required temperature from +19 to +22°C.

Snail preparation is one of the most important steps. Since the stomach of the snail may contain earth, it should be prepared in the manner described above. As a result, after 5 days, during which only water is available to the snails, the body of the snail is completely cleansed, which ensures the production of a high quality powdered extract.

2. Extraction of the snail.

The prepared snail is mechanically removed from the shell and placed in a homogenizer with the addition of a small amount of water, the amount of water is 30% by weight of the snails. Homogenization is carried out until a mixture of homogeneous composition is obtained.

3. Drying.

A homogeneous and homogeneous mass is dehydrated. For this, a spray dryer with a temperature of 40-45°C or by lyophilization (lyophilization) can be used. Drying can be carried out within 24-26 hours.

4. Sterilization.

The sterilization cycle consists of loading the sterilization chamber and the actual sterilization process. The average sterilization cycle is 20-30 minutes, depending on the chosen method (device).

As a result, a fine powder of light gray or gray color is obtained (see photo in Fig. 1-2), which retains the maximum amount of useful substances.

The table shows the nutrient content (calorie, protein, fat, carbohydrate, vitamins and minerals) per 100 grams of powdered extract.

Nutrient Quantity calories 90 kcal Squirrels Fats Carbohydrates Water Ash vitamins Vitamin A, RE 30 mcg Retinol 0.03 mg Vitamin B1, thiamine 0.01 mg Vitamin B2, riboflavin 0.12 mg Vitamin B4, choline 65 mg Vitamin B6, pyridoxine 0.13 mg Vitamin B9, folate 6 mcg Vitamin B12, cobalamin 0.5 mcg Vitamin E, alpha tocopherol, TE 5 mg Vitamin K, phylloquinone 0.1 µg Vitamin PP, NE 1.4 mg Macronutrients Potassium, K 382 mg Calcium Ca 10 mg Magnesium 250 mg Sodium, Na 70 mg Sulfur, S 161 mg Phosphorus, P 272 mg trace elements Iron, Fe 3.5 mg Copper, Cu 400 mcg Selenium, Se 27.4 mcg Zinc, Zn 1 mg Sterols (sterols) Cholesterol 50 mg Saturated fatty acids Saturated fatty acids 14:0 Myristic 16:0 Palmitic 18:0 Stearic Monounsaturated fatty acids 16:1 Palmitoleic 18:1 Oleic (omega-9) Polyunsaturated fatty acids 18:2 Linoleic 18:4 Stioride Omega-3 20:5 Eicosapentaenoic (EPA), Omega-3 Omega 3 fatty acids 22:5 Docosapentaenoic (DPA), Omega-3 Omega 6 fatty acids

Source: Chemical composition and nutrition analysis: USDA National Nutrient Database for Standard Reference https://fdc.nal.usda.gov/ndb/search/list.

Implementation example

According to the claimed method, a powdered extract was obtained from grape snails, which is a fine powder of light gray color (see photo in Fig. 1-2). The resulting extract has high quality characteristics: homogeneity, fine fraction, dry matter content, useful substances, vitamins and trace elements of the grape snail (Helix pomatia) are preserved in it to the maximum.

Thus, the claimed method ensures the production of a high-quality powdered extract, as well as an increase in the efficiency and manufacturability of the extract production process, and a reduction in time and energy costs during its production.

The analysis of the prior art showed that the claimed set of essential features set forth in the claims is unknown. This allows us to conclude that the claimed technical solution complies with the condition of patentability "novelty".

Comparative analysis showed that in the prior art no solutions were found that have features that coincide with the distinctive features of the claimed invention, and the knowledge of the influence of these features on the technical result has not been confirmed. Thus, the claimed technical solution satisfies the condition of patentability "inventive step".

The above information confirms the possibility of using the claimed technical solution for obtaining a high quality powdered extract from the grape snail, which can be used in medicine, pharmacology and the food industry, and therefore meets the patentability condition "Industrial applicability".

Claims (5)

1. A method for obtaining a powdered extract from a grape snail, including the following steps: preparation: the snail placed in the cage is provided with flour and water for 3-5 days, after which the snail is provided with only water for 5 days, keeping at a temperature of 19-22 ° WITH; then the prepared snail is mechanically removed from the shell and placed in a homogenizer, to which water is added in an amount of 30% by weight of the snails; after which the homogeneous and homogeneous snail mass is dried and further sterilized. 2. The method according to p. 1, characterized in that the drying is carried out in a spray dryer at a temperature of 40-45°C. 3. The method according to p. 1, characterized in that the drying is carried out by lyophilization. 4. The method according to p. 1, characterized in that the drying is carried out within 24-26 hours. 5. The method according to p. 1, characterized in that the sterilization is carried out for 20-30 minutes.

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