RU2758066C1 - Remedy for the treatment of escherichiosis and citrobacteriosis of young cattle in early postnatal ontogenesis - Google Patents

Abstract

FIELD: veterinary medicine.SUBSTANCE: invention relates to the field of veterinary medicine, in particular to a means for the prevention and treatment of infectious gastrointestinal diseases of young cattle in early postnatal ontogenesis, and can be used in agriculture for the treatment of bacterial diseases - Escherichiosis and cytrobacteriosis caused by enterobacteria. The product contains Lactulose, Lactobacillus acidophilus (B-4107) K-1-T, Enterococcus faecium UDS 86 and distilled water in specified quantities.EFFECT: reduction of production losses of young cattle.1 cl, 1 dwg, 4 ex

Description

The technical field to which the invention relates

The invention relates to the field of veterinary medicine, in particular, to an agent for the prevention and treatment of infectious gastrointestinal diseases of young cattle in early postnatal ontogenesis and can be used in agriculture for the treatment of bacterial diseases (escherichiosis and cytrobacteriosis) caused by enterobacteria (E coli and C. feundii).

State of the art

There is a known drug for the prevention of diarrhea in young farm animals (US Pat. E. coli strains. The toxoid was obtained by treating a broth culture of E. coli with a 0.4% formalin solution for two weeks, followed by centrifugation and collection of the supernatant.

The disadvantages of this drug is that the risk of intoxication is possible.

A known drug for the treatment of Escherichiosis in calves (dis. Work. Doctor of Veterinary Sciences. Setdekov R.A. Development of new means of specific prevention and treatment of Escherichiosis in calves and piglets, Kazan 2015, http://www.ksavm.senet.ru/ files / avtoref / setdekov / setdekov_disser.pdf) characterized by the fact that calves with milk or colostrum are given a tincture of dried vegetable raw materials of scumpia and sumac, which is used in an amount of 20 ml per 1 kg of body weight 2-3 times a day for 5 days with simultaneous intramuscular administration of Tetravit at 3 ml / head with an interval of 5 days, while to obtain the tincture, the initial components are taken in the following ratio, wt. %:

collection of scumpia and sumac 4-6,

ethyl alcohol 18-22,

sodium chloride 2.5-3.5,

distilled water 70-74.

The disadvantages of this remedy are the short duration of action and the chemical load.

The closest in technical essence and the achieved positive result, taken by the authors as a prototype, is a means for the prevention of Escherichiosis in young cattle, including combined probiotics for prophylactic Escherichiosis in cattle. Based on strains Bifidobacterium 48 bifidum DSM 20456, ATCC 29521 and Enterococcus faecium UDS 86. (dissertation work of candidate of veterinary sciences Vasiliev N.V. Preventive measures for escherichiosis of young cattle in the Stavropol Territory, Savropol 2017, http: // www .stgau.ru / science / dis / avtoreferat / vasilev_2017 / 1.pdf)

The disadvantage of this remedy for the treatment of escherichiosis of young cattle is that a large amount of drunk milk can get into the rumen, which will result in the development of toxic dyspepsia.

Brief description of drawings and other materials

FIG. 1 - Microbiota of the large intestine in calves in early postnatal ontogenesis.

Disclosure of invention

The objective of the present invention is to develop an agent for the treatment of dysbiosis of the gastrointestinal tract in young cattle in early postnatal ontogenesis, which has a high efficiency of treatment against bacterial diseases (Escherichiosis and cytrobacteriosis) caused by enterobacteriaceae (E. coli and C. feundii), characterized by ecological safety and obtaining products of high sanitary quality.

The technical result, which can be achieved with the help of the present invention, is reduced to a decrease in production losses of young cattle, using a drug with a smaller volume for oral administration, due to the targeted ingestion of the proposed drug into the large intestine and acceleration of the sanogenesis process.

The technical result is achieved using a means for the treatment of escherichiosis and cytrobacteriosis of young cattle in early postnatal ontogenesis containing Enterococcus faecium UDS 86, while additionally contains distilled water, Lactulose, Lactobacillus acidophils (B-4107) K-1-T, mixed when temperature of 37 ° C, in the following ratio of components:

distilled water - 5 ml,

Lactulose - 2.5 g

Enterococcus faecium UDS 86 - 10 × 10 ⁹ -10 × 10 ¹² KOE / 5 ml,

Lactobacillus acidophilus (B-4107) K-1-T - 20 × 10 ⁹ - 10 × 10 ¹² CFU / 5 ml.

Thus, Escherichiosis is an infectious disease of young animals of most types of farm animals, caused by the pathogen Escherichia coli, which occurs more often in an acute form and is accompanied by diarrhea. The disease causes significant economic damage to livestock. The costs of organizing the zoo-veterinary rules for keeping, feeding and operating animals are a trigger for the onset of the disease. The source of infection is sick, recovered animals, contaminated environmental factors. Calves are infected in the first 3-7 days of life through the surrounding objects contaminated with E. coli: colostrum, milk dishes, air, hands and overalls of service personnel, as well as through contact with murine rodents.

Cytrobacteriosis is an acute infectious disease of the digestive system caused by bacteria of the genus Citrobakter, characterized by a decrease in the viability of the animal against the background of dehydration. Citrobakter is a widespread microorganism in nature and is present in soil, water and faeces of humans and animals, therefore it is often transmitted through the alimentary route.

The causative agents of dysbiosis of the gastrointestinal tract cause a decrease in appetite, depression, calves lie down, involuntary convulsions are observed, sniff the stomach, trying to hit it. Diarrhea appears after 1-2 days. The feces are watery, yellow, with different shades with an unpleasant odor. The result of which can be mass death of young cattle in early postnatal ontogenesis. Modern remedies have a large volume of liquid to be drunk, which must be given to the calf in a certain time, so that the drug does not get into the rumen. Accordingly, in case of a mass disease, carrying out therapeutic measures by this method requires a lot of labor and time. To eliminate the above disadvantages, a method is proposed for the treatment of calves in early postnatal ontogenesis of patients with Escherichiosis or Citrobacteriosis, which includes:

- Enterococcus faecium (Streptococcus faecium) UDS 86 2 × 10 ⁹ - 10 × 10 ¹² CFU / ml - gram-positive oval cocci, located singly, in pairs or in short chains. Colonies on M17 are round, small, and white. Used to combat dysbiosis of farm animals, producer of vitamin B;

- Lactobacillus acidophilus (B-4107) K-1-T 3 × 10 ⁹ - 10 × 10 ¹² CFU / ml - gram-positive facultative anaerobic bacteria located singly or in short chains. Antagonist of putrefactive and pyogenic bacteria.

- Lactulose (Lactulose) C12H22O11 - a disaccharide consisting of galactose and fructose, not hydrolyzed by disaccharidases of the mucous membrane of the small intestine. It has the property of breaking down the intestinal flora of the colon and is a breeding ground for beneficial bacteria in the large intestine. Dilution of 0.5 g per 1 ml of distilled water.

Implementation of the invention

Examples of the specific implementation of obtaining an agent for the treatment of escherichiosis and cytrobacteriosis in young cattle in early postnatal ontogenesis.

Example 1. When examining 12 calves in early postnatal ontogenesis, clinical signs were revealed: decreased appetite, depression, calves lie down, involuntary convulsions, sniff the belly, trying to hit it. Then the calves were divided into 4 groups of 3 calves each. Of these, 3 groups are experienced and one is a control group. Diarrhea appears after 1-2 days. The feces are watery, yellow, with different shades with an unpleasant odor. To establish the disease, fecal samples are taken from the rectum from sick animals that have not been treated with antibiotics. The resulting material contained representatives of the Enterobacteriaceae family (Escherichia coli and C. Feundii, Fig. 1)

For the treatment of calves with a negative shift of the conditionally pathogenic microflora, distilled water, Lactulose, Enterococcus faecium UDS 86 and Lactobacillus acidophilus (B-4107) K-1-T are mixed in a bottle at a temperature of 37 ° C, in the following ratio of the components:

Distilled water - 5 ml,

Lactulose - 0.9 g

Enterococcus faecium UDS 86 - 10 × 10 ⁵ CFU / 5 ml,

Lactobacillus acidophilus (B-4107) K-1-T - 20 × 10 ⁵ CFU / 5 ml.

Calves are drunk with the prepared agent 2 hours before feeding once every 48 hours in the form of an aqueous solution at the rate of 5 ml per 50 kg of live body weight for the treatment of colibacillosis and colibacillosis. After that, the general condition of the animal is assessed daily in comparison with the animals of the control groups. Also, an assessment was made of changes in the live weight of calves and temperature indicators during the entire experiment, and the microbial landscape of the contents of the gastrointestinal tract was studied. According to the results, it was established that profuse diarrhea passes into the formalized feces 26 hours after taking the drug, but due to the fact that there was not enough Lactulose volume for the development of microflora specified by the complex drug, E. coli suppressed the beneficial microflora, resulting in diarrhea , dehydration and death on the 3rd day (Fig. 1) .. Percentage of deaths in the group: 75% of the experimental group.

Example 2. Treatment of calves is carried out in accordance with example No. 1 in the following ratio of the components:

Distilled water - 5 ml,

Lactulose - 1.5 g

Enterococcus faecium UDS 86 - 10 × 10 ⁷ CFU / 5 ml,

Lactobacillus acidophilus (B-4107) K-1-T - 20 × 10 ⁷ CFU / 5 ml.

The prepared product is given 2 hours before feeding once every 48 hours in the form of an aqueous solution at the rate of 5 ml per 50 kg of live body weight. According to the results of daily monitoring, it was found that profuse diarrhea passes into formalized feces after 17-20 hours after taking the drug, and confident posture is also observed. (Fig. 1).

Example 3. Treatment of calves is carried out in accordance with example No. 1 in the following ratio of the components:

Distilled water - 5 ml,

Lactulose - 2.5 g

Enterococcus Raecium UDS 86 - 10 × 10 ⁹ CFU / 5 ml,

Lactobacillus acidophilus (B-4107) K-1-T - 20 × 10 ⁹ CFU / 5 ml.

Calves are drunk with the prepared product 2 hours before feeding once every 24 hours in the form of an aqueous solution at the rate of 5 ml per 50 kg of live body weight. According to the results of which it was found that profuse diarrhea passes into the formalized feces 12-15 hours after taking the drug, there is a lack of impaired coordination of movement. In this experiment, there were no dead animals (Fig. 1).

Thus, the most optimal option for the treatment of calves at the rate of 5 ml per 50 kg of live body weight is the following ratio of components:

Distilled water - 5 ml,

Lactulose - 2.5 g

Enterococcus faecium UDS 86 - 10 × 10 ⁹ KOE / 5 ml,

Lactobacillus acidophils (B-4107) K-1-T - 20 × 10 ⁹ KOE / 5 ml.

As a result of the targeted ingestion of the proposed drug in the large intestine, the process of sanogenesis is accelerated due to the competing inhibition of pathogenic microorganisms.

Example 4. An agent for the treatment and prevention of calves against dysbiosis of the gastrointestinal tract was tested for toxicity in rabbits. The toxicity of the agent is determined with a single cutaneous application of the solution to sexually mature, heterosexual white rabbits weighing 3.0-3.5 kg.

For the experiment, 5 experimental rabbits were used, on the skin of which the developed drug was introduced by the method of scarification. After treatment, the general condition of the experimental animals was monitored for 2 weeks. No changes were found on the skin. Not a single animal died.

In the course of testing the proposed agent on calves with negative shifts of opportunistic microflora, its high efficiency was proved as new methods of prevention, environmental safety and, as a consequence, acceleration of the process of sanogenesis. The tool is highly effective in dysbiosis of the gastrointestinal tract of newborn calves, helps to normalize the functioning of the digestive system, there is a formalized feces, normalization of body temperature, improved appetite, no bloating, there is a confident standing posture, weight gain.

The proposed invention in comparison with the prototype and other known technical solutions has the following advantages:

- target ingestion of the proposed drug in the large intestine and acceleration of the process of sanogenesis due to the competing inhibition of pathogenic microorganisms;

- high efficiency of treatment;

- environmental safety of the substance;

- elimination of a negative shift in the conditionally pathogenic microflora of the gastrointestinal tract.

Claims (5)

A remedy for the treatment of escherichiosis and citrobacteriosis of young cattle in early postnatal ontogenesis, containing Enterococcus faecium UDS 86, characterized in that it additionally contains distilled water, Lactulose, Lactobacillus acidophilus (B-4107) K-1-T, mixed at 37 ° C, in the following ratio of components: distilled water - 5 ml, Lactulose - 2.5 g Enterococcus faecium UDS 86 - 10 × 10 ⁹ -10 × 10 ¹² CFU / 5 ml, Lactobacillus acidophilus (B-4107) K-1-T - 20 × 10 ⁹ -10 × 10 ¹² CFU / 5 ml.

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