RU2730459C1 - Bactericidal agent - Google Patents

Abstract

FIELD: chemistry.SUBSTANCE: invention relates to a bactericidal agent containing hydrogen peroxide, silver nitrate, 5 % solution of furocoumarin in ethyl alcohol, water, wherein the disclosed bactericidal agent further contains glutaric aldehyde, with the following ratio of components, wt%: hydrogen peroxide – 5; silver nitrate – 1; 5 % solution of furocoumarin in ethyl alcohol – 10; glutaric aldehyde – 0.3; water – balance.EFFECT: described is a bactericidal agent.1 cl, 1 ex, 2 tbl

Description

The invention relates to the field of hygiene and sanitation and can be used for disinfection and sanitation of the air environment of closed (livestock) premises.

Known means for disinfecting the air, containing the ratio of components, wt. %: hydrogen peroxide - 5; silver salt - 1; 5% solution of furocoumarin in ethyl alcohol - 10; water - the rest (RF patent No. 2699372) [1].

The disadvantages of the invention is that the agent does not have sufficiently high antibacterial properties.

The objective of the present invention is to improve the efficiency of the process of disinfection of indoor air.

The technical result of the proposed invention is to increase the antimicrobial activity of the active substance.

The task and the specified technical result are achieved due to the fact that in the known bactericidal agent containing hydrogen peroxide, soluble silver salt, 5% solution of furocoumarin in ethyl alcohol, water, according to the invention, glutaraldehyde is additionally contained in the following ratio of components, wt%: hydrogen peroxide - 5; silver salt - 1; 5% solution of furocoumarin in ethyl alcohol - 10; glutaraldehyde - 0.3; water is the rest.

Hydrogen peroxide has a universal antimicrobial effect. Gram-positive and gram-negative bacteria, viruses, and many types of pathogenic fungi are sensitive to it. Causes the death of spores of the majority of spore-forming bacteria (Matreninskaya ES A new generation of disinfectants based on hydrogen peroxide / Medical alphabet. Epidemiology and sanitation, 2011, No. 1, p. 40) [2].

A wide spectrum of antimicrobial action of silver, low toxicity, lack of resistance to it in most pathogenic microorganisms and non-allergic silver make it possible to use it as an effective, harmless disinfectant (Kulsky L.A. Silver water. - 9th ed., Revised and add. - K .: Naukova Dumka, 1987. - 134 p.) [3].

Known antibacterial activity of derivatives of plant furocoumarin - peucedanin, obtained from the Morison mountaineer (Shirokikh I.V., Burova L.G., Lipeeva A.V., Schultz E.E. Study of the antibacterial properties of peucedanin derivatives against Staphylococcus aureus in vitro. / I. V. Shirokikh, L. G. Burova, A. V. Lipeeva, E. E. Schultz // Siberian Medical Bulletin. - 2018. - No. 2. - P. 8-12.) [4].

Furocoumarins obtained from grapefruit juice inhibit the biofilm formation of Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa (Girennavar B., Cepeda ML, Soni KA, Vikram A., Jesudhasan P., Jayaprakasha GK, Pillai foucoufouc SD, juice Patil BS inhibits autoinducer signaling and biofilm formation in bacteria // Int. J. Food Microbiol. 2008, V. 125, P. 204-208) [5].

Glutaraldehyde is an effective fast-acting biocide used for disinfection and regulation of the growth of microorganisms (RF patent No. 2534574) [6].

A disinfectant was prepared by mixing the listed ingredients in the indicated ratios (wt%). The drug Ammifurin was used as a source of furocoumarins. Of the silver salts, silver nitrate was used.

An example of the use of a bactericidal agent.

In a closed room, a bactericidal agent was sprayed into the air. Spraying can be carried out using any device that allows you to dose the amount of sprayed substance.

The total microbial contamination was determined by the aspiration method using the Krotov apparatus. Air samples were taken to determine its bacterial contamination before and after air disinfection. To determine the content of yeast-like and mold fungi, inoculation was performed on Sabouraud's medium, total microbial contamination on plain agar. The air flow rate was 25 l / min for 40 min. The seeded media were kept in a thermostat at (37 ± 1) ° C for 24 h, then at room temperature for 24 h, and then the grown bacterial colonies were counted and the colony-forming units (hereinafter referred to as CFU) contained in 1 m ^{3 of} air were calculated. ...

In the process of air treatment with the proposed tool, a decrease in the total microbial contamination and molds was noted (tables 1, 2).

The total microbial contamination when using the proposed bactericidal agent is reduced by an average of 1.3 times compared with the use of the known method, the content of molds - by 1.33 times.

As can be seen from the tables, after the application of the proposed bactericidal agent, the total microbial contamination of the premises decreases by an average of 5.58 times, the content of molds - by 13.33 times.

Claims (6)

A bactericidal agent containing hydrogen peroxide, silver nitrate, a 5% solution of furocoumarin in ethyl alcohol, water, characterized in that it additionally contains glutaraldehyde, with the following ratio of components, wt%: hydrogen peroxide - 5 silver nitrate - 1 5% solution of furocoumarin in ethyl alcohol - 10 glutaraldehyde - 0.3 water is the rest.