RU2716082C1 - Olive oil enriching method with fucoxantin - Google Patents

Abstract

FIELD: fat and oil industry.

SUBSTANCE: invention relates to the fat and oil industry. Method for enriching olive oil with fucoxantin involves producing an alcohol extract from brown algae of the genus Cystoseira containing fucoxanthin. Mixing in equal proportions of alcohol extract, distilled water, olive oil with addition of 2 mg of table salt, stirring during 10 minutes with subsequent separation of oil fraction and washing with distilled water.

EFFECT: invention allows to produce olive oil enriched with fucoxantin suitable for use both in pure form, and in production of food products and biologically active substances.

1 cl, 1 ex

Description

The invention relates to the oil industry and is intended to produce a functional food product, namely, pure vegetable oil with a high content of fucoxanthin (FC).

A known method of enriching vegetable oils with biologically active substances zeaxanthin and lutein [1], including drying and grinding of raw materials, extraction of lutein and zeaxanthin diesters with hot ethanol, removal of ethanol by drying, and addition of the obtained active components to vegetable oils. The disadvantage of this method is that it does not allow to obtain vegetable oils enriched in fucoxanthin.

Closest to the claimed is a method of enriching vegetable oil with fucoxanthin (US Pat. 2629276, RF, IPC A23D 9/00, 2019), in which the process of obtaining a biologically active product based on vegetable oil enriched in FC includes the following steps: enriched in FC alcohol extract, previously obtained from the raw biomass of diatom Cylindrotheca closterium, mixed with vegetable oil in the ratio of alcohol extract: oil 1: 5, then the mixture is kept for 60 minutes, followed by separation of the oil from insoluble alcohol oic fraction. As vegetable oil, use sunflower or olive oil. The disadvantage of this method is the low yield of FC (75%) when using raw C. closterium biomass as a raw material for obtaining alcoholic extraction of fucoxanthin, as well as in the duration of the fraction separation process (60 min). The disadvantage is also that when changing the raw material base and attracting, for example, algae of the genus Cystoseira as a source of fucoxanthin, the known method does not give the expected result.

The objective of the invention "Method for the enrichment of olive oil with fucoxanthin" is the development of technology that allows the use of brown algae of the genus Cystoseira to obtain an alcoholic extract as a source of biologically active substances fucoxanthin to saturate vegetable oil.

The technical result consists in the fact that the method allows to expand the raw material base for obtaining fucoxanthin, to reduce the saturation time of vegetable oils Fc extracted from brown algae of the genus Cystoseira, to obtain a biologically active product based on plant materials with a high content of Fc (0.28 mg / ml oil )

The problem is solved in that in the Method for the enrichment of olive oil with fucoxanthin, including saturation of the oil with the pigment fucoxanthin by mixing in a certain ratio of oil and alcohol extract from algae, an alcohol extract containing fucoxanthin obtained from brown algae of the genus Cystoseira is mixed in equal proportions with distilled water , olive oil with the addition of 2 mg of sodium chloride, stirred for 10 minutes, followed by separation of the oil fraction and washing with distilled water

In common with the prototype is the preliminary isolation of alcohols of FC alcohol from biomass of algae, as well as the saturation of vegetable oil with fucoxanthin by mixing in a certain ratio of vegetable oil with an alcohol extract enriched in fucoxanthin.

New in the claimed method is that the raw materials for the selection of FC are brown algae of the genus Cystoseira. Also new is the fact that the conversion of FC from an alcoholic extract to oil is carried out by mixing in equal proportions the alcoholic extract of FC, vegetable oil, distilled water, with the addition of edible salt.

Brown algae of the genus Cystoseira are a valuable raw material for the production of biologically active substances, including FC, since they contain a sufficient amount for isolation. FC has antioxidant, cytostatic, hypotensive, antidiabetic, antiviral effects [2, 3]. Reception of fucoxanthin in an amount of 0.6 mmol per day has a preventive effect against cancer and can fight obesity [4, 5]. Currently, a number of brown algae processing technologies have been developed with the aim of obtaining various therapeutic and preventive additives and dietary supplements. A sufficiently high total biomass of these algae in the Black Sea makes them a promising object for industrial use.

The redistribution of carotenoids between the oil and alcohol fractions occurs upon mixing the alcohol extract Fc obtained from brown algae of the genus Cystoseira, distilled water and olive oil. The authors experimentally found that the addition of distilled water is necessary to dissolve sodium chloride, dilute alcohol and transfer FC from the alcohol fraction to the oil fraction. The lack of water did not allow to obtain the desired result. The addition of 2 mg of sodium chloride contributes to the rapid saturation of the oil fraction with fucoxanthin. Washing the oil fraction with distilled water is necessary to clean it from salt. The redistribution of FC between the oil and alcohol fraction depends on the volume ratio of the alcohol extract: oil: distilled water in the presence of sodium chloride. The most effective was mixing in equal proportions of the alcoholic extract of FC, vegetable oil and distilled water, then the oil is saturated with fucoxanthin within 10 minutes, and the output of FC from the alcohol fraction to the oil is 80%.

Example

Alcoholic extract of FC was preliminarily obtained from brown algae of the genus Cystoseira by extraction of the Black Sea cystozira with ethyl alcohol in accordance with the description of the patent [6]. To 20 ml of alcoholic extract with FC (pigment content 0.35 mg / ml) was added 20 ml of distilled water and 20 ml of olive oil, then 2 mg of sodium chloride was added and the mixture was stirred for 10 min. An oil fraction was formed with a fucoxanthin content of 0.28 mg / ml. The fraction was separated and washed with 40 ml of distilled water to obtain a product enriched in fucoxanthin in oil and free of alcohol. The result was 20 ml of olive oil enriched with FC with a high content of 0.28 mg / ml. The concentration of FC in the oil fraction was determined using thin-layer chromatography on silica gel in a solvent system of hexane: acetone (7: 3). The silica gel layer containing FC was extracted with 96 ° alcohol. Then spectrophotometrically on a UNICO 1201 spectrophotometer at a wavelength of 450 nm. The yield of FC from the alcohol fraction to the oil fraction was 80%. The resulting oil had a transparent brown color with a reddish tint, a homogeneous consistency, the taste of olive oil, with a slight smell of fish oil.

The oil has good organoleptic properties, contains a high concentration of FC (0.28 mg / ml), the method of its production is quite simple and economical. The method can be applied to obtain environmentally active additives based on vegetable oils enriched in fucoxanthin at the enterprises of the food and pharmaceutical industries.

References taken into account.

1. Pat. 2424722 RF. IPC A23D 7/00 / Method for the production of vegetable oils enriched with diesters of lutein and zeaxanthin. L.A. Deineka, V.I. Deineka, M.Yu. Tretyakov, I.A. Gostishchev, T.Yu. Semkina. Applicant and patent holder: Belgorod State University State Educational Institution of Higher Professional Education. - No.2010110710 / 13, declared 03/23/2010, publ. 07/27/2011, bull. No. 21. - 7 p.

2. Mikami K. Biosynthetic pathway and health benefits of fucoxanthin, an algae-specific xanthophyll in brown seaweeds / K. Mikami, M. Hosokawa // Int. J. Mol. Sci. - 2013 .-- Vol. 14, iss. 7.-P. 13763-13781.

3. Ryabushko V.I. Functional role of fucoxanthin and phytohormones in brown algae / V.I. Ryabushko, L.I. Musatenko, L.V. Voytenko, E.V. Popova, M.V. Nechoroshev // Intern. J. on Algae. - 2015. - Vol. 17, iss. 1. - P. 68-81.

4. Hosokawa M. Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARgamma ligand, troglitazone, on colon cancer cells / M. Hosokawa, M. Kudo, H. Maeda, H. Kohno, T. Tanaka, K. Miyashita // Biochim. Biophys. Acta. - 2004 .-- Vol.1675. - P. 113-119.

5. Maeda H. Fucoxanthin from edibleseaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues / H. Maeda, M. Hosokawa, T. Sashima, K. Funayama, K. Miyashita // Biochem. Biophys. Res. Commun. - 2005. - Vol. 332. - P. 392-397.

6. Pat. 2658705 Russian Federation. IPC A23L 17/60 Method for producing a prophylactic product from the Black Sea algae of the genus Cystoseira (options) / Nekhoroshev M.V., (RU), Ryabushko V.I. (RU), Gureeva E.V. (RU); Applicant and patent holder Federal State Budgetary Institution of Science A.O. Institute of Marine Biological Research Kovalevsky RAS ”- No. 2016140006; declared 10/11/2016, publ. 06/22/2018 Bull. Number 18.

Claims (1)

A method of enriching olive oil with fucoxanthin, comprising saturating the oil with the pigment fucoxanthin by mixing in a certain ratio of oil and alcohol extract from algae, characterized in that the alcohol extract containing fucoxanthin obtained from brown algae of the genus Cystoseira is mixed in equal proportions with distilled water, olive oil with by adding 2 mg of sodium chloride, stirred for 10 minutes, followed by separation of the oil fraction and washing with distilled water.