RU2704857C1 - Strain of lactobacilli lactobacillus plantarum - producer of lactic acid, antibiotic substances and starter component for production of fermented milk products - Google Patents

Abstract

FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Strain Lactobacillus plantarum Lb (n)37 2-2018, having antagonistic activity in relation to the opportunistic and pathogenic microflora, is deposited in the Russian National Collection of Industrial Microorganisms under registration number VKPM V-13052. Strain of bacteria Lactobacillus plantarum VKPM V-13052 can be used to produce a probiotic starter.EFFECT: invention allows to increase the milk coagulation speed, reduce the manufacturing process time and increase the product shelf life.1 cl

Description

The invention relates to biotechnology, in particular, can be used in the food industry.

A known bacterial strain Lactobacillus amylovorus BT - 24/88 VKPM B-6253 isolated from the contents of the cecum of a milk pig [Tarakanov B.V. The bacterial strain LACTOBACILLUS AMYLOVORUS, used to produce the probiotic lactoamilovorin. Patent RU No. 2054478, publ. 02.20.1996, declared 5063804/13, 10/01/1992] used for the preparation of the veterinary probiotic preparation "Lactoamilovorin".

The disadvantages of this strain is the low level of antagonistic activity against different types of opportunistic enterobacteria.

However, this strain has a lower antagonistic activity compared to the proposed strain.

The technical solution is the selection of a highly productive new local strain of lactobacilli by selection.

EFFECT: obtaining a pure culture of the lactic acid microorganism Lactobacillus plantarum VKPM B-13052, isolated from the surface of clover grass from the highlands above the village of Koban in North Ossetia-Alania by repeated inoculations on nutrient media.

The identification of the strain was carried out according to the method of Bannikova L.A. and PCR diagnostics.

Stages and results of genetic research.

a) Sequencing portions of the sequence encoding the 16S rRNA gene.

b) Sequence analysis of the gene encoding 16S rRNA.

Initial screening using a database of GenBank and RDP-II showed that the studied strain belongs to the following systematic groups of Bacteria; Firmicutes Bacilli; Lactobacillales; Enterococcaceae; Enterococcus.

According to the results of the analysis of sequencing of variable regions of genes encoding 16S rRNA and analysis of phylogenetic relationship constructed using typical strains of closely related bacteria, the test strain is closest to the species Lactobacillus plantarum (99%) and deposited in the All-Russian collection of industrial microorganisms (VKPM) of the State Research Institute of Genetics and Genetics under the number B-13052.

Properties of the strain Lactobacillus plantarum VKPM B-13052:

- rod-shaped cells arranged in chains;

- on the surface of agarized milk hydrolyzate and MRS-agar after 24 hours at a temperature of 37 ° C forms point colonies.

- milk fermentation rate of 6 hours.

- the limit of acid formation is 145 ° T.

- products synthesized by the strain - lactic acid and antibiotic substances.

- the diameter of the zone of inhibition of growth of Staphylococcus aureus - 30 mm;

- the diameter of the zone of inhibition of growth of Escherichia coli is 29 mm.

The antibiotic activity of the strain of Lactobacillus plantarum VKPM B-13052 was determined on a solid nutrient medium (MPA) by agar diffusion.

The method, conditions and composition of the media for long-term storage of the strain - lyophilization; storage at a temperature of 4-6 ° C; culture media: MRS; Rogosa et.al .; milk with chalk; milk with yeast autolysate, glucose, litmus, liver extract and CaCO ₃ .

The method, conditions and composition of the media for the propagation of the strain - at a temperature of 37 ° C - environment: MRS; skim milk, unsalted cheese whey with a content of at least 4.0% sugar.

Optimum conditions and composition of the medium for fermentation - temperature 37 ° C; Wednesday: MRS, unsalted cheese whey with a content of at least 4.0% sugar; milk of farm animals.

The proposed new strain of Lactobacillus plantarum VKPM B-13052 isolated from the surface of an inflorescence of creeping clover growing in the highlands above the village of Koban in North Ossetia-Alania is well adapted to local conditions. It is unpretentious in cultivation and storage, has a low marginal acidity and a high rate of milk coagulation, which allows to increase economic efficiency in production (by speeding up the production process and increasing the shelf life of the finished product).

The strain exhibits a high antagonistic ability in relation to opportunistic and pathogenic microflora.

The proposed new strain of lactobacilli Lactobacillus plantarum VKPM B-13052 has high biological activity and is the basis for obtaining probiotic starter culture.

Claims (1)

The strain of lactobacilli Lactobacillus plantarum VKPM B-13052 is a producer of lactic acid and antibiotic substances and a starter component for the production of probiotic products.