RU2649135C1 - Method for increase of adaptation to physical loads and body restoration by "alloplant" biomaterial - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method involves subcutaneous injection of dispersed Alloplant biomaterial diluted in physiological saline in a ratio of 50 mg of biomaterial per 12 ml of solution to specific biologically active points responsible for regulating the body energy balance GV4, GV14, GV20, GV23, BL24, BL26, LI11, ST36, as well as to additional points that are previously identified in the process of acupuncture diagnostics and that have reduced electrical conductivity. Injections are performed by 2-12 sessions per course, with an interval of 1-5 days between sessions.

EFFECT: application of the invention allows to improve the health of athletes in a short time due to activation of antioxidant systems against the background of metabolic processes normalization.

2 ex

Description

The invention relates to medicine, in particular to restorative and sports medicine, and can be used to increase the health of the body in conditions of physical and psychological stress, including during intensive training.

Excessive physical activity, characteristic of modern sport, has a negative impact on the health status of athletes. The body’s universal response to stress is the activation of lipid peroxidation (LPO). With excessive accumulation of active forms of oxygen, peroxides and their metabolites, a condition occurs that is called oxidative stress. Excessive activation of free radical chain oxidation of lipids can lead to the accumulation of products such as lipoperoxides, fatty acid radicals, ketones, aldehydes, keto acids in the tissues, which, in turn, leads to damage and increased permeability of cell membranes. The participation of free radicals in the pathogenesis of many diseases is proved: atherosclerosis; impaired cerebral, coronary and peripheral circulation; diabetes mellitus and diabetic angiopathy; rheumatoid, inflammatory and degenerative diseases of the musculoskeletal system; eye damage; pulmonary diseases; oncological pathology and premature aging. Excessive activation of lipid peroxidation has a negative effect on the contractile capabilities of the muscular activity of athletes as a result of difficulties in transmitting motor nerve impulses; the damaging effect of lipid peroxidation inevitably leads to a deterioration in the relaxation properties of muscles, leads to a decrease in the aerobic energy supply of muscle work, to the loss of many important substances by muscle cells, and generally negatively affects the athlete’s physical performance and endurance.

Currently, various means are used to prevent the fatigue and maintain physical performance under conditions of increased loads of high-performance sports, which can increase the capacity of the antioxidant system of the body.

The most relevant among them are medicines, dietary supplements and innovative methods for the correction of factors that limit the performance of athletes who are not related to doping, are not toxic substances and do not cause side effects in the training process and competitive activity.

In clinical practice, various substances are widely used that have antioxidant properties of both natural (ascorbic acid, tocopherol, carotenoids, flavonoids, selenium, superoxide dismutase, catalase), and synthetic origin (ionol, probucol, unitiol, emoxipin). The disadvantage of the substances used as antioxidants is their instability, fat solubility and the large size of the molecules, which complicates their introduction into the body and subsequent penetration through biological membranes.

A known method of improving the psychoemotional state of the body of athletes (RU No. 2469730, IPC A61K 35/30, publ. 12/20/2012), which consists in pharmacological effects on the body through the intranasal effect of a nootropic drug - cortexin after a preliminary psychotherapeutic effect. The method allows to improve the psycho-emotional state of athletes, but the effect on the functional state of the body, the overall physical performance of the athletes is not disclosed.

There is a method of increasing the physical performance of athletes (RF patent No. 2542506, IPC АКК 35/36, publ. 02.20.2015), which proposes the use of the Esporin dietary supplement based on reindeer antlers. The composition of Esporin includes phospholipids, amino acids, microelements, water and fat-soluble vitamins, esters of unsaturated fatty acids, esters of organic acids, sets of prostaglandins and peptides, because of this, in addition to antioxidant effects, this drug carries the risk of developing an allergic reaction to any of the components .

The well-known "Method for the correction of thyroid status and health of highly qualified athletes who are disturbed by intense physical activity of a cyclic orientation" (RF patent No. 2485948, IPC A61K 31/375, published on June 27, 2013). The method involves the simultaneous administration of two drugs: “Selenium-active” and “Ribose” during a period of intense physical exertion and allows you to regulate the production of thyroid hormones by activating the functions of the antioxidant system and inhibiting free radical processes. The disadvantages of the method include the possible presence of contraindications for taking drugs that increase the activity of the thyroid gland.

The well-known "Method of stimulating the body's adaptation to extreme and stressful factors" (RF Patent No. 2108789, А61К 31/79, publ. 04/20/1998), which is provided by intramuscular or intragastric administration in subtoxic doses of aqueous solutions of a composition containing highly dispersed metallic silver and poly -N-vinylpyrrolidone (PVP). The disadvantages of the method include the fact that it was tested only on laboratory animals in which the reaction to taking the drug may differ from human.

The well-known "Method for the treatment of adaptation disorders" (RF Patent No. 2472432, IPC АВВ 5/05, published on January 20, 2013), which includes transcranial electrical stimulation, trance guidance against the background of aromatherapy and relaxation music, reflexotherapy effect on corporate points and zones of Su-Jok as well as herbal remedies, neuroprotective agents, adaptogens or other allopathic and homeopathic preparations. The method, comprehensively affecting the processes of self-regulation, allows you to reduce the dose of the main medication and, accordingly, avoid undesirable complications. The disadvantage of this method is the low industrial applicability, due to the complexity of the combination of various therapeutic techniques.

As you know, one of the effective physical factors used in rehabilitation is acupuncture, which has an anti-inflammatory, analgesic effect, improves microcirculation and lymph circulation, metabolic processes and tissue metabolism. This creates a favorable background for the correction of the adaptive capabilities of the central nervous system, which has a beneficial effect on the body as a whole.

Known acupuncture stimulation with Alloplant biomaterial for the treatment and prevention of diseases by exposure to biologically active points and zones (RF Patent No. 2238076, IPC A61K 35/32, publ. 20.10.2004). The Alloplant biomaterial in an amount of 10-100 mg is diluted in 5-15 ml of physiological saline and injected into biologically active points with the aim of their long-term stimulation and prolongation of the effect. The method is aimed at restoring the underlying mechanisms of nervous regulation, metabolic processes, increasing immunity. The well-known regenerative properties of the Alloplant biomaterial, made on the basis of crushed biological tissues with metered-extracted glycosaminoglycans (Registration certificate No. 901 of July 22, 1987), have provided it with widespread use in surgery for the correction of fibrotic and degenerative-dystrophic changes in tissues (RF Patent No. 2189257, “Alloplant biomaterial for regenerative surgery”, IPC A61L 27/00, A01N 1/00, publ. September 20, 2002). A special form in the form of dispersed Alloplant biomaterial is used for injection (“Alloplant Biomaterials for Regenerative Surgery. Catalog”, under the editorship of Prof. ER Muldashev, Ufa, 2007). All biomaterials of the Alloplant series have extremely low antigenicity and zero toxicity, so their use has no contraindications.

The disadvantages of the method are the lack of a targeted effect on the antioxidant system, respectively, in the low efficiency of the recovery of the body and adaptation to physical activity.

The objective of the invention is to develop an effective and affordable way to increase the body's adaptation to extreme and stress factors, increasing efficiency and helping to restore the body after physical and emotional stress, devoid of the above disadvantages. The technical result of the invention is to reduce lipid peroxidation by activating the functions of the antioxidant system.

The problem is solved, and the technical result is achieved by increasing adaptation to physical activity and restoring the body by subcutaneous injection of dispersed Alloplant biomaterial, diluted in physiological solution in the ratio of 50 mg of biomaterial to 12 ml of solution, to specific biologically active points responsible for regulation energy balance of the body GV4, GV14, GV20, GV23, BL24, BL26, LI11, ST36, as well as at additional points that are previously identified in the process of acupuncture urn diagnosis, and having reduced electrical conductivity indicators, and the injection is carried out sessions in an amount of 2-12 per course of 1-5 days with an interval between sessions.

The achievement of the technical result is due to the following factors. When injecting Alloplant biomaterial according to the proposed acupuncture recipe, which is an optimal combination of specific biologically active points, it was first used its ability to influence the change in the direction of metabolic processes towards anabolic reactions and increase the power of the antioxidant system, which helps to increase the adaptive reserves of the body. In the course of the studies conducted by the authors, it was first established that the Alloplant biomaterial at the intracellular level promotes the activation of cell membrane receptors with a subsequent increase in the intracellular concentration of cyclic adenosine monophosphate that regulates cellular metabolism. When acupuncture is introduced, the Alloplant biomaterial helps to reduce free radical oxidation by increasing the activity of enzymes of the antioxidant system - catalase and glutathione peroxidase, which resulted in a decrease in lipid peroxidation products - diene conjugates and Schiff bases. It was also found to increase the oxygen capacity of the blood, an increase in hemoglobin and total blood protein to normal values, which eliminates the development of complications associated with blood clotting. This circumstance excludes Alloplant from the category of doping.

The individually used additional points for injecting the Alloplant biomaterial according to the essence of the invention are determined using diagnostics according to R. Voll’s method, based on measuring electrical resistance at biologically active representative points - control points of classical Chinese meridians (A.V. Samokhin, Yu.V. Gotovsky. Electropuncture diagnostics and therapy according to the method of R. Voll - M., Imedis, 2012 - S. 48-53).

The frequency of injections with an acceptable interval between sessions of 1 to 5 days was determined experimentally and correlates with the results of studies on the processes of resorption in the tissues of Alloplant biomaterials (R. Khasanov. Injection forms of Alloplant allografts. and biological activity ”, Perm, 1999, cand.).

Clinical example 1

Athlete - single-combatant A-s Bulat, 29 years old, specialization - Brazilian Jiu-Jitsu, sports experience - 14 years. The intensity of physical activity is high. The total amount of training load is 20 hours per week. Complaints of fatigue, headaches, discomfort in the heart, decreased interest in training and to achieve results.

An objective examination: pulse - blood pressure - 140/90, 48 beats / min, ECG sinus bradycardia, signs of left ventricular hypertrophy, signs of impaired repolarization of the posterior wall of the left ventricle. Clinical blood test: er. 4.9 × 10 ¹² / l; HB - 143 g / l; lake. 5.2 × 10 ⁹ / l (E-2, P-3, S-65, L-26, M-4. Total protein 68 g / l, cholesterol - 4.3 mmol / l; LDH -315 E / L; CPK - 174 U / L; glucose - 5.1 mmol / L; diene conjugates - 3.85 μmol / L, Schiff bases - 0.40 μmol / L, catalase - 11.85 conventional units / g. During acupuncture diagnostics according to R. Voll’s method on the computerized hardware-software complex IMEDIS-EXPERT (version 7.61), underestimated electrical conductivity was detected at representative points of the pericardium meridians (42 conventional units) and a triple heater (37 conventional units) responsible for the endocrine system, as well as the phenomenon of "falling stra Lki ”on the heart meridian. The athlete was diagnosed with overtraining syndrome and prescribed treatment according to the proposed method: Alloplant biomaterial of 0.5 ml was injected into general regulatory points according to the developed recipe, as well as at additional points determined by the acupuncture diagnosis of this patient. group trays of yan-meridians: TE8, located on the channel of the triple heater and GB39 on the channel of the gallbladder, affecting the autonomic regulation of the body, pair agreement points BL14 (ШУ-dot and the pericardium) and BL15 (ШУ-point of the heart), HT3 points on the meridian of the heart. The patient tolerated the procedures satisfactorily, already on the third day noted the effect in the form of increased efficiency. 7 sessions were conducted, with a frequency of 3 times a week. As a result of the treatment, the athlete's fatigue decreased, general weakness and discomfort in the heart area disappeared, headaches stopped, sleep and appetite returned to normal, and high motivation for playing sports and achieving results returned.

An objective examination: blood pressure 120/75, pulse 52 beats / min. On the ECG, a clear positive trend in the form of improved repolarization processes. Clinical blood test: er. 5.2 × 10 ¹² / l; HB - 154 g / l; lake. 4.8 × 10 ⁹ / l (E-1, P-2, S-61, L-27, M-9). Total protein 79 g / l, cholesterol - 3.7 mmol / l; LDH - 242 U / L; KFK - 92 U / L; lactate - 1.8 mmol / l; glucose - 4.4 mmol / l; diene conjugates - 3.55 μmol / l, Schiff bases - 0.36 μmol / l, catalase - 17.65 conventional units / g. Acupuncture diagnostics according to R. Voll showed the restoration of the electrical conductivity of all representative points to normal values (from 50 to 65 conventional units).

Clinical example 2

Athlete - Oleg Oleg B, 22 years old, footballer, sports experience - 11 years. The intensity of physical activity is high. The total amount of training load is 24 hours a week. Training regimen - 2 times a day, 6 days a week. Complaints of fatigue, irritability, unstable mood, loss of appetite, discomfort in the epigastrium. Objectively: blood pressure 110/86, pulse 65 beats / min, on ECG: slowing of atrial conduction, incomplete blockade of the left leg of the bundle of His. Clinical blood test: er. 5.1 × 10 ¹² / l; HB - 145 g / l; white blood cells 5.0 × 10 ⁹ / l (E-1, P-2, S-61, L-29, M-7). Biochemical analysis of blood: total protein 72 g / l, cholesterol - 6.75 mmol / l; LDH - 303 U / L; KFK - 118 U / L; lactate - 4.5 mmol / l; glucose - 6.2 mmol / l; diene conjugates - 3.88 μmol / L, Schiff bases - 0.41 μmol / L, catalase - 11.55 conventional units / g. Cardiointervalometry indices indicated the predominance of sympathetic influences (stress index (IN) 128, variational range (BP) 365, Fashion (M) 764); orthostatic index 2.1 rel. reflected overfatigue. Adaptation Potential Index R.M. Bayevsky (1987) 3.1 - testified to the tension of adaptation mechanisms.

Acupuncture diagnostics according to R. Voll’s method revealed low electrical conductivity at representative points of the heart meridian (26 conventional units), the phenomenon of “falling arrows” on the liver and blood circulation meridians (pericardium), and overestimated gastric meridians (76 conventional units). ) and gall bladder (81 conventional units).

The athlete was treated with a course of 6 procedures with a frequency of 2 times a week according to the proposed method: Alloplant biomaterial was injected in 0.5 ml at acupuncture points according to the developed recipe and at additional points according to the results of acupuncture diagnostics: at agreement points BL15 (ШУ-point heart), BL14 (ШУ-point of the pericardium), BL18 (ШУ-point of the liver), BL19 (ШУ-point of the gallbladder), BL21 (Stomach joke); GB24 on the gallbladder channel and VC12 on the anteromedian meridian, responsible for the work of the liver and stomach.

According to the results of the treatment, the athlete noted a surge of strength and efficiency, general weakness, irritability disappeared, mood and sleep stabilized, appetite improved.

Objectively: blood pressure 120/80, pulse 62 beats / min. Clinical blood test: er. 4.9 × 10 ¹² / l; HB - 150 g / l; white blood cells 5.8 × 10 ⁹ / l (E-1, P-2, S-62, L-28, M-7). Total protein 79 g / l, cholesterol - 4.7 mmol / l; LDH - 240 U / l; KFK - 48 U / l; lactate - 2.1 mmol / l; glucose - 5.1 mmol / l; diene conjugates - 3.39 μmol / l, Schiff bases - 0.37 μmol / l, catalase - 18.33 arb./g. Indicators of cardiointervalometry: IN 103, variational range (BP) 426, Fashion (M) 842 reflected the balanced tone of the autonomic nervous system; orthostatic index 1.3 rel. and index of adaptive potential R.M. Bayevsky (1987) 2,4 - met the parameters of satisfactory adaptation. The conductivity of all representative points were within the physiological norm.

Thus, the proposed method of adaptation to high physical exertion and recovery of the body with Alloplant biomaterial allows improving the health of athletes in a rather short time and with high efficiency due to the activation of antioxidant systems against the background of normalization of metabolic processes, which makes it possible to recommend it for widespread use.

Claims (1)

A way to increase adaptation to physical activity and restore the body by subcutaneous injection of dispersed Alloplant biomaterial, diluted in physiological solution in a ratio of 50 mg of biomaterial per 12 ml of solution, to specific biologically active points responsible for regulating the energy balance of the body GV4, GV14, GV20 , GV23, BL24, BL26, LI11, ST36, as well as to additional points that are previously detected during acupuncture diagnostics, and having reduced conductivity and, moreover, injections are carried out in sessions of 2-12 per course, with an interval between sessions of 1-5 days.