RU2611826C1 - Composition for diluting and storing male dog sperm - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to veterinary and biology. Medium for dilution and storing male dog sperm contains TRIS, citric acid, fructose, human albumen, water for injections, dog blood serum, enrogen.

EFFECT: invention makes it possible to preserve viability of sex gametes of male dogs for a long period of time, and protects them from temperature shock.

Description

The invention relates to veterinary medicine and biology, specifically to animal reproduction, allows you to dilute and save freshly obtained sperm of dogs in deep freezing.

German researchers found that the introduction of bovine serum albumin into the medium for storing chilled sperm of boars helps to increase the survival time of spermatozoa outside the body, inhibits the capacitation reaction and protects their acrosome [2]. A comparative test of the effect of bovine serum albumin, porcine serum albumin and ovalbumin on the mobility and preservation of the acrosome in boar sperm at 15 ° C showed that bovine serum albumin has a higher protective effect. According to other researchers, bovine serum albumin enhances the fertilizing ability of stored sperm of boars [3] and has an antioxidant effect on the membrane structure of sperm [4].

The prior art composition for freezing dog sperm based on distilled water, sodium citrate, dextrose, penicillin, streptomycin, catalase, yolk, glycerin and dog blood serum (RU 2001128885 A, A61D 19/02, publication date 20.08.2003). This composition is selected as the closest analogue.

The claimed invention is specific and easy to prepare. TRIS, citric acid and fructose are quite widely used as components for preparing media for diluting the seminal fluid of various animals. The proposed composition includes the use of a 10% solution of human albumin and dog blood serum as a substitute for chicken yolk. The composition of the claimed medium allows you to keep viable sex gametes of dogs for a long period of time, and protects them from temperature shock.

The resulting medium can be used for long-term storage of dog semen at a temperature of -198 ° C. The proposed composition of the medium is characterized by ease of preparation, low cost.

The composition of the medium:

TRIS - 2.4 g

Citric Acid - 1.3 g

Fructose - 1.0 g

Human Albumin 10% - 20 ml

Water for injection - 72.2 ml

Dog blood serum - 0.1-0.2 mcg

Enrogen - 25 mg.

The medium is prepared as follows: TRIS - 2.4 g, citric acid - 1.3 g, fructose - 1.0 g are weighed on an analytical balance. These components are placed in a flask, 20 ml of 10% human albumin solution and 72.2 ml are added. and water for injection and 0.1-0.2 μg of dog blood serum, then mix thoroughly until the components are completely dissolved, to prevent bacterial contamination, add 25 mg of enrogen.

BIBLIOGRAPHY

1. Waberski D., Weitze K.F., Rath D. Wirkung von bovinem scrumalbumin and Zwitterionen buffer auf flussigkonservierten Ebrsamen // Zuchthugiene, 1989 .-- v. 24. - No. 3. - p. 128-133.

2. Weitze K.F. Interaction between inseminate and ovarian function in the saw. // Reprod. Domest. Anim. - 1990. - v. 25. - No. 4. - p. 191-196.

3. Upreti G.C., Oliver J.E., Duganzich D.M. e.a. Development of a chemically defined ram semen diluent (RSD-1). // Animal Reproduction Sci. 1995. - v. 37. - p. 143-157.

Claims (1)

The composition of the medium for dilution and storage of male semen containing TRIS - 2.4 g, citric acid - 1.3 g, fructose - 1.0 g, human albumin 10% - 20 ml, water for injection - 72.2 ml, serum dog blood - 0.1-0.2 mcg, enrogen - 25 mg.