RU2611827C1 - Medium for dilution and storage of sperm of male dogs - Google Patents

Abstract

FIELD: agriculture; veterinary medicine.

SUBSTANCE: medium for dilution and storage of male dog sperm comprises TRIS - 6.06 g, citric acid - 3.4 g, fructose - 2.5 g, glycerol - 16 ml, human albumin 10% - 40 ml, water for injection - 184 ml.

EFFECT: when the fresh sperm of male dogs is diluted with the proposed medium the sperm cells remain viable and active movement for 6 days from the day of dilution.

Description

The invention relates to veterinary medicine and biology, namely to animal reproduction.

German researchers found that the introduction of bovine serum albumin into the medium for storing chilled sperm of boars helps to increase the survival time of spermatozoa outside the body, inhibits the capacitation reaction and protects their acrosome [1]. A comparative test of the effect of bovine serum albumin, porcine serum albumin and ovalbumin on the mobility and preservation of acrosome in boar sperm at 15 ° C showed that bovine serum albumin has a higher protective effect.

According to other researchers, bovine serum albumin enhances the fertilizing ability of preserved sperm of boars [2] and has an antioxidant effect on the membrane structures of sperm [3].

The claimed invention is distinguished by its specificity and ease of preparation. TRIS, citric acid, glycerin and fructose are quite widely used as components for preparing media for diluting the seminal fluid of various animals. The proposed composition is characterized by the use of a 10% solution of human albumin as a substitute for chicken yolk, which allows you to dilute and maintain the freshly obtained sperm of dogs at a temperature of + 4 ° C. The proposed environment allows you to keep viable sexual gametes of dogs for 6 days and protects them from temperature shock. The resulting medium can be used for short-term storage of dog semen at a temperature of + 4 ° C. The proposed composition of the medium is characterized by ease of preparation, low cost.

The composition of the medium:

1. TRIS - 6.06 g

2. Citric acid - 3.4 g

3. Fructose - 2.5 g

4. Glycerin - 16 ml

5. Human albumin 10% - 40 ml

6. Water for injection - 184 ml

Wednesday is prepared as follows. TRIS - 6.06 g, citric acid - 3.4 g, fructose - 2.5 g are weighed on an analytical balance. These components are placed in a flask, 40 ml of 10% human albumin solution, 16 ml of glycerin and 184 ml of water for injection are added. , then mix thoroughly until the components are completely dissolved.

When diluting freshly obtained sperm of males with the proposed sperm medium, when stored properly, they retain their viability and active movement for 6 days from the day of dilution.

BIBLIOGRAPHY

1. Waberski D., Weitze K.F., Rath D. Wirkung von bovinem scrumalbumin and Zwitterionen buffer auf flussigkonservierten Ebrsamen // Zuchthugiene, 1989 .-- v. 24. - No. 3. - p. 128-133.

2. Weitze K.F. Interaction between inseminate and ovarian function in the saw. // Reprod. Domest. Anim. - 1990. - v. 25. - No. 4. - p. 191-196.

3. Upreti G.C., Oliver J.E., Duganzich D.M. e.a. Development of a chemically defined ram semen diluent (RSD-1). // Animal Reproduction Sci. 1995. - v. 37. - p. 143-157.

Claims (1)

Male sperm dilution and storage medium containing TRIS - 6.06 g, citric acid - 3.4 g, fructose - 2.5 g, glycerin - 16 ml, human albumin 10% - 40 ml, water for injection - 184 ml , when diluting freshly obtained sperm of males with this medium, the sperm retain their viability and active movement for 6 days from the day of dilution.