RU2611822C1 - Composition of medium for dilution and storing male dog sperm - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to veterinary and biology, in particular to composition of medium for dilution and storing male dog sperm. Medium contains TRIS - 6.06 g, citric acid - 3.4 g, fructose - 2.5 g, glycerin - 16 ml, human albumen 10% - 40 ml, dog blood serum 0.1-0.2 mcg, enrogen - 25 ml, water for injections - 184 ml.

EFFECT: application of the invention makes it possible to preserve viability of sex gametes of male dogs for a long period of time and protect them from temperature shock.

Description

The invention relates to veterinary medicine and biology, specifically to animal reproduction, allows you to dilute and save freshly obtained sperm of dogs in deep freezing.

German researchers found that the introduction of bovine serum albumin into the medium for storing chilled sperm of boars helps to increase the survival time of spermatozoa outside the body, inhibits the capacitation reaction and protects their acrosome [1]. A comparative test of the effect of bovine serum albumin, porcine serum albumin and ovalbumin on sperm motility and acrosome safety at 15 ° C showed that bovine serum albumin has a higher protective effect. According to other researchers, bovine serum albumin enhances the fertilizing ability of the preserved sperm of boars [2] and has an antioxidant effect on the membrane structures of sperm [3].

The prior art composition for freezing dog sperm based on distilled water, sodium citrate, dextrose, penicillin, streptomycin, catalase, yolk, glycerin and dog blood serum (RU 2001128885 A, A61D 19/02, publication date 20.08.2003). This composition is selected as the closest analogue.

The proposed composition is specific and easy to prepare. TRIS, citric acid, glycerin and fructose are quite widely used as components for preparing media for diluting the seminal fluid of various animals. The claimed invention includes the use of a 10% solution of human albumin and dog blood serum as a substitute for chicken yolk, as well as the use of enrogen, an antibiotic with a wider spectrum of action.

The proposed composition of the medium is characterized by ease of preparation, low cost.

The composition of the medium:

TRIS - 6.06 g

Citric Acid - 3.4 g

Fructose - 2.5 g

Glycerin - 16 ml

Human Albumin 10% - 40 ml

Dog blood serum 0.1 - 0.2 mcg

Enrogen - 25 mg

Water for injection - 184 ml

The composition of this medium allows you to keep viable gametes of dogs for a long period of time and protects them from temperature shock.

The medium is prepared as follows: TRIS - 6.06 g, citric acid - 3.4 g, fructose - 2.5 g are weighed on an analytical balance. These components are placed in a flask, 40 ml of 10% human albumin solution, 16 ml of glycerol are added, 0.1-0.2 μg of dog serum, 25 mg of enrogen and 184 ml of water for injection are then thoroughly mixed until the components are completely dissolved.

When diluting freshly obtained sperm of males with the claimed medium, spermins, if stored properly, retain their viability and active movement for a long period of time. The resulting medium can be used for long-term storage of dog semen at a temperature of -198 ° C.

Information sources

1. Waberski D., Weitze K.F., Rath D. Wirkung von bovinem scrumalbumin and Zwitterionen buffer auf flussigkonservierten Ebrsamen // Zuchthugiene, 1989 .-- v. 24. - No. 3. - p. 128-133.

2. Weitze K.F. Interaction between inseminate and ovarian function in the saw. // Reprod. Domest. Anim. - 1990. - v. 25. - No. 4. - p. 191-196.

3. Upreti G.C., Oliver J.E., Duganzich D.M. e.a. Development of a chemically denned ram semen diluent (RSD-1). // Animal Reproduction Sci. 1995. - v. 37. - p. 143-157.

Claims (1)

The composition of the medium for dilution and storage of male semen containing TRIS - 6.06 g, citric acid - 3.4 g, fructose - 2.5 g, glycerin - 16 ml, human albumin 10% - 40 ml, dog blood serum 0.1-0.2 μg , enrogen - 25 mg, water for injection - 184 ml.