RU2603088C1 - Method for stimulating growth and development of plants - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Method comprises a treatment of seeds by a biological preparation, represented by culture of Enterococcus durans VKPM V-10093 lactic bacteria, mixed with beet molasses at a preset ratio. Treatment of seeds by a biological preparation is carried out in an amount of 10⁹ cells per 1 kg of seeds.

EFFECT: invention allows increasing productivity of crops and reducing their incidence rate.

1 cl, 4 tbl, 1 ex

Description

The invention relates to biotechnology, in particular to means for producing a new biological preparation for stimulating the growth of agricultural plants.

There is a method in which a bacterial strain is used to stimulate plants (patent No. 2051586 dated January 10, 1996, IPC A01 63/00, C12N 1/20).

However, the known strain is used on industrial, grain and vegetable crops, providing high growth rates, significantly exceeding the original data (without processing), but not used on forage grasses.

The known method, where they also use a biological product to stimulate and protect plants (patent No. 2478290 from 11.11.2011, IPC A01N 63/02, A01C 1/06, C12N 1/20).

However, in the known method at the level of biological product use an additional stimulator humate, which increases the cost of implementing the method.

The closest technical solution is the method in which the treatment is carried out before sowing, enveloping the seeds with a mixture of biological product and molasses at a temperature of 28-30 ° C for 60-64 hours (patent No. 2421967 from 06/27/2011, IPC А01С 1/06).

The disadvantage of the prototype method is that additionally cultivate the biological product on the liquid fraction of the post-alcohol stillage stillage, which complicates the technical solution. In addition, the Enterococcus durans strain VKPM B-10093 as a plant stimulant was not found in the known technical solutions.

The technical result is a simplification of the method and the expansion of technological capabilities of the strain Enterococcus durans VKPM B-10093.

The technical solution of the claimed object is that before sowing seeds, a biological product is prepared from the Enterococcus durans VKPM B-10093 strain, which contains 10 ⁹ microbial cells in 1 ml. For processing 1 kg of seeds, 10 ml of this drug is used.

The method is as follows.

Before sowing, a culture mixture of Enterococcus durans VKPM B-10093 is prepared on beet molasses with a microbial number of 10 ⁹ and is used in an amount of 10 ml per 1 kg of seeds. The seeds are treated with this mixture to adhere the lactobacillus cells to the seeds. Then the seeds are dried to flowability. 10 ml of beet molasses is taken per 1 ml of lactobacilli culture. Cultivation of Enterococcus durans VKPM B-10093 is carried out for 5-6 hours at t 37 ° C.

The strain Enterococcus durans VKPM B-10093 (patent No. 2461620 dated 09/20/2012), used as a plant stimulant and cultivated on molasses - a waste product of sugar production, increases plant productivity, increases leaf mass and winter hardiness of plants, reduces plant morbidity.

The isolated strain from the terrestrial mass of the culture of clover meadow (Trifolium pratense L.), growing in the mountain zone of natural phytocenosis, has unique properties to stimulate the growth and development of not only this type of clover, but also of other agricultural crops (timothy grass meadow, winter wheat and others).

Cells are in the form of gram-positive cocci, with a diameter of up to 0.3 mm, an optional anaerobic. Using molasses to increase the activity of the biostimulant - Enterococcus durans strain, the winter hardiness of winter crops, as well as perennial grasses (clover, timothy sown in autumn) increases simultaneously.

Usually, meadow clover is sown in the early spring. In our experiments, clover seeds were processed in the fall (first decade of November).

When the biostimulator was applied in the control plot (without seed treatment), single shoots were observed, and in the experimental plot, about 90 pieces were recorded on 1 m ² , 23 pieces in winter wheat. on the control and about 50 pcs. in the area where the seeds were enveloped in a strain mixed with molasses.

Example. 1 ml of a pure Enterococcus durans culture containing 10 ⁹ cells was introduced into 100 ml of beet molasses and cultured at t 37 ° C for 5-6 hours to 10 ⁹ microbial cells in 1 ml. The microbial number was determined in a Goryaev chamber under a microscope. After treatment with a biological product based on beet molasses, the seeds were dried at a temperature of 30 ° C in a thermostat until they flow. Dosed seeds of 3 crops: meadow clover, meadow timothy and winter wheat (Zustrich cultivar of Ukrainian selection) were sown in areas of 5 m ^{2 of} each plant species in triplicate. Untreated strain seeds were sown with the same area as a control.

Sowing was carried out on November 10, 2014. Germination and plant development were taken into account before leaving for the winter. The following year, the growth of crops, the phases of development and ripening of seeds in winter wheat were noted. Timothy has a phase of formation of sultans, flowering; clover has budding and flowering, foliage and the number of generative inflorescences on the stem.

All experimental cultures studied the disease.

The results of the experiments are summarized in tables 1, 2, 3, 4.

From the above data in tables 1, 2, 3, 4 it follows that the strain Enterococcus durans VKPM B-10093 helps to increase plant productivity, reduce the incidence of feed and grain plants.

Claims (1)

A method of stimulating the growth and development of plants, including pre-sowing seed treatment with a biological product containing cells of a microorganism strain, characterized in that the Enterococcus durans VKPM B-10093 lactobacillus culture mixed with beet molasses is used as a biological product, and seed treatment with a biological product is carried out at a rate of 10 ⁹ cells per 1 kg of seeds.