RU2587722C2 - Collection having antihypoxic and hypotensive actions - Google Patents

Abstract

FIELD: medicine; pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry, namely to collection having antihypoxic and hypotensive actions. Collection having antihypoxic and hypotensive actions and containing hawthorn berries, chamomile flowers, peppermint leaves, rose hips berries, is characterised by comprising chokeberries, meadowsweet herb, red clover flowers, lady's mantle leaves and hawthorn flowers at a certain ratio.

EFFECT: collection has a pronounced anti-hypoxic and hypotensive actions and can be used for preventing hypoxic damages, hypertension and cerebrovascular disease (CVD).

1 cl, 3 dwg

Description

The invention relates to medicine, namely to herbal medicine, and can be used for the prevention of hypoxic damage, arterial hypertension and cerebrovascular accident (NMC).

Charges from medicinal plants are widespread for the prevention of diseases, since they do not have undesirable side effects, are easier to tolerate by the human body than many synthetic products, and have a mild to moderate effect.

Known drug from the ginkgo biloba plant with antihypoxic effect, which is used for the prevention and treatment of NMC (Shah ZA, Nada SE, Dore S. Heme oxygenase 1, beneficial role in permanent ischemic stroke and in Gingko biloba (EGb 761) neuroprotection // Neuroscience . - 2011. - Vol. 180. - P.248-255).

However, the known drug has a significant drawback, as it is made from a relic plant, which grows in vivo only in two small areas in eastern China.

Known collection that has a hypotensive effect (RF patent No. 2155058), which contains rose hips of a blood-red fruit, motherwort five-bladed grass, horsetail field grass, dried grass, shepherd's purse grass and birch leaves.

The disadvantage of this collection is the bitter taste, due to the presence in its composition motherwort five-bladed and horsetail. In addition, the well-known collection has a pronounced sedative effect due to the motherwort five-lobed and cinnamon, which limits its use during working hours, when driving vehicles and in operator activity.

The closest technical result achieved is the collection of medicinal plants for the prevention and treatment of cerebrovascular accidents, which has an antihypoxic effect (RF patent №2338550), which contains periwinkle small leaves, peppermint leaves, plantain large leaves, hawthorn fruits, rosehip fruits, mountain ash fruits , common wormwood grass, sweet clover grass, dried grass marshmallows, pharmacy chamomile flowers, licorice roots.

A disadvantage of the known collection is that periwinkle is a cultivated plant that grows in natural conditions only in the Mediterranean and West Asia, in addition, this plant contains more than 40 types of alkaloids whose therapeutic doses are close to toxic or are associated with toxic effects. Due to the toxicity of the periwinkle, it is necessary to strictly observe the dosage, with its overdose, inhibition of cardiac activity occurs. Also a significant drawback of the known collection is the bitter taste, due to the presence in the composition of wormwood ordinary grass, clover grass, plantain large leaves and vinca small leaves.

The technical result of the proposed collection is to expand the arsenal of fees of plant origin for the prevention of cerebrovascular accidents.

The technical result is achieved by the fact that the collection additionally contains the chokeberry fruit chokeberry, meadowsweet herb, clover meadow flowers, common cuffs of common leaves and hawthorn flowers in the following ratio of medicinal plants in parts:

Aronia of Aronia 2 Straw grass four Hawthorn flowers and fruits 3 Clover meadow flowers 2 Chamomile Pharmacy Flowers 3 Peppermint leaves 2 Rosehip fruit 2

Common cuff leaves 2

The invention is illustrated by figures 1, 2, 3 in the form of graphs.

Figure 1 shows the result of HPLC water infusion of the claimed collection, where 1 is gallic acid, 2 is gallic acid esters, 3 is rutin, 4 is hyperoside, 5 is kempferol glycoside, 6 is luteolin glycoside, 7 is a mixture of ferulic and coumaric acids, 8 - quercetin glycoside.

Figure 2 presents the results of a study of the antihypoxic effect of the claimed collection in an experiment on rats.

Figure 3 presents the results of a study of the hypotensive effect of the claimed collection in an experiment on volunteers.

The claimed collection contains the chokeberry fruit chokeberry, meadowsweet herb, hawthorn flowers and fruits, meadow clover flowers, pharmacy chamomile flowers, peppermint leaves, rosehip fruits, common cuff leaves with a ratio of components in parts of 2: 4: 3: 2: 3, respectively: 2: 2: 2. In the claimed collection of medicinal plants are selected in such a way and in such a ratio as to obtain a high effect in the prevention of NMC.

Aronia of aronia fruits affect the hemostatic system, lengthening the coagulation time and reducing the rate of fibrin polymerization in human blood plasma. The mixture of procyanidins, anthocyanins and phenolic acids contained in the chokeberry is one of the most powerful natural antioxidants.

Lubaznika vulva herb has an anxiolytic effect, antioxidant effect and reduces the severity of exudation during inflammation.

Hawthorn flowers and fruits have a protective effect on ischemia, which is due to flavonoids, namely oligomers of proanthocyanides, which are concentrated in berries and inflorescences. Hawthorn also contains vitamins P and C, which have a capillary-strengthening effect. Modern studies of the effects of hawthorn extract on an experimental model of stroke show that it reduces the output of pro-inflammatory cytokines, the expression of intercellular adhesion molecules, as well as the inflammatory response and leukocyte infiltration in the focus of ischemia.

Clover meadow flowers improve the lipid profile, reducing the level of triglycerides, total cholesterol and LDL in plasma due to the content of coumarins.

Chamomile pharmacy flowers have antispasmodic, anti-inflammatory and antioxidant properties, as well as inhibit COX-2 and reduce the synthesis of prostaglandin E2, but do not reduce the expression of COX-1.

Peppermint leaves have antimicrobial, antioxidant and antispasmodic effects.

Rosehip fruits contain flavonoids, vitamins, tannins and have an antioxidant effect, and the water rosehip extract contains a high level of vitamin C.

Cuffs of common leaves have a pronounced antioxidant effect, in addition, they increase the concentration of phospholipids in the membranes of red blood cells and improve microcirculation due to phenolic compounds.

The collection is prepared as follows. Each type of dry raw material that meets the requirements of the State Pharmacopoeia X and XI edition is crushed to a particle size of not more than 7 mm. Then the dry raw materials are weighed and thoroughly mixed in equal parts until a uniform mixture is filtered and dust is sieved through a sieve with a hole diameter of 0.18 mm. To prepare the aqueous extraction, 20.0 g of dry raw material is poured into 200 ml of purified water at room temperature, insisted on a water bath for 15 minutes, then cooled at room temperature for 45 minutes and filtered.

The claimed collection is taken 2 times a day (morning and evening) after meals for three weeks in the form of water infusion.

Contraindications to admission are allergic reactions to herbal components that are part of the proposed collection, pregnancy, breastfeeding.

In the water infusion of the claimed collection contains: gallic acid and its esters, rutin, hyperoside, kempferol glycoside, luteolin glycoside, a mixture of ferulic and coumaric acids, quercetin glycoside (figure 1). These substances determine the therapeutic effect of drug collection: gallic acid inhibits Ca-activated chlorine channels, has a cardioprotective effect; rutin and ferulic acid inhibit ACE, increase the level of nitric oxide; quercetin and catechin inhibit angiotensin-II-induced redox-dependent signaling pathway in smooth muscle cells with hypertension; luteolin inhibits the proliferation of smooth muscle cells in blood vessels.

To prove the effectiveness of the proposed collection, a study was conducted on laboratory animals.

The study was carried out on 100 white male rats of the Wistar line, weighing 141.4 ± 26.2 g. The animal line was grown in a vivarium of the Institute of Cytology and Genetics SB RAS (Novosibirsk). All animals were randomly divided into eight groups: the 1st group of rats was injected with 1 ml of decoction into the stomach through a probe for three days, the 2nd group was given boiled water in the stomach in the same volume for three days, the 3rd group was injected 1 ml of broth for seven days, group 4 was injected with water in the same volume for seven days, group 5 was injected with 1 ml of broth for 14 days, group 6 was injected with water in the same volume for 14 days, 7 the 1st group was injected with 1 ml of broth for 21 days, the 8th group was injected with water in the same volume for 21 days.

On the next day after the last injection, resistance to acute hypobaric hypoxia was determined. Hypobaric hypoxia was modeled using a special pressure chamber, in which, using a vacuum electric pump, the necessary air pressure was created, corresponding to a "height" of 11500 m above sea level. During the simulation of acute hypoxia in an animal, the time of loss of posture (VPP) and the time of life (VL) were recorded. Runway - the period from the moment of reaching a critical height to the moment the animals lose their ability to maintain a normal posture. VZh - the period from the moment of reaching a critical height to the appearance in the animal of a second agonal inspiration.

Statistical processing of the obtained material was carried out using the program “Statistica” v 6.0. When processing the results, the non-parametric Mann-Whitney test was used, since the values of the studied parameters did not obey the normal distribution. When comparing the groups, an acceptable level of significance of conclusions about the significance of differences was p <0.05.

With 3-, 7-, and 14-day administration of decoction from the herbs, the runways and VZh did not significantly differ between the experimental and control groups.

Significant antihypoxic effect was observed in rats that were injected with decoction of herbs for 21 days (figure 2). The graph shows that the life time of rats that were given decoction of herbs for 21 days was, on average, 1.5 times longer compared to the control group. Moreover, the time of posture loss in the experimental and control groups did not differ significantly.

The data obtained indicate that in the claimed collection of medicinal plants are selected in such a way and in such a ratio that they can increase resistance to hypoxia when applying the collection for 21 days.

Since resistance to hypoxia depends on the state of the cardiovascular system, the effect of the claimed collection on blood pressure in humans was investigated. The study of the hypotensive effect of the collection was carried out on 24 volunteers, who were divided into two equal groups. The subjects of the experimental group took 200 ml of decoction before the study, and the control - 200 ml of boiled water. Then a continuous non-invasive measurement of blood pressure was carried out using the beat to beat method for 30 minutes on a CNAP ™ Monitor 500 with registration of systolic, diastolic, medium pressure and pulse trends.

Statistical processing of the obtained material was carried out using the program “Statistica” v 6.0. When processing the results, the non-parametric Mann-Whitney test was used, since the values of the studied parameters did not obey the normal distribution. When comparing the groups, an acceptable level of significance of conclusions about the significance of differences was p <0.05.

When monitoring blood pressure in the group of people taking the decoction, there was a significant decrease in systolic pressure, while in the control group there was an increase in systolic pressure (Fig. 3).

Thus, the claimed collection, made from inexpensive affordable raw materials, significantly increases resistance to hypoxia, has a hypotensive effect and can be used to prevent cerebrovascular disorders.

Claims (1)

A collection with antihypoxic and hypotensive effects, containing hawthorn fruit, chamomile pharmacy flowers, peppermint leaves, rosehip fruit, characterized in that it additionally contains chokeberry aronia, meadowsweet herb, clover meadow flowers, cuff ordinary leaves and hawthorn flowers in the following ratio medicinal plants in parts:
Aronia of Aronia 2 Straw grass four Hawthorn flowers and fruits 3 Clover meadow flowers 2 Chamomile Pharmacy Flowers 3 Peppermint leaves 2 Rosehip fruit 2 Common cuff leaves 2

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