RU2338550C1 - Admixture of herbs for prevention and treatment of disturbances of cerebral circulation - Google Patents

Abstract

FIELD: medicine; pharmacology.

SUBSTANCE: admixture contains leaves of periwinkle, leaves of mint peppery, leaves of common plantain, hawthorn foetuses, rose hips, mountain ash foetuses, a grass of felon herb, sweet clover, a grass of cotton weed, camomile flowers, roots of liquorice at a proportion of components in parts accordingly 1:1:1:1:1:1:1:2:1:1:1.

EFFECT: admixture dilates an arsenal of medical products of a phytogenesis with the expressed therapeutic effect for treatment of disturbances of a cerebral circulation.

5 dwg, 3 dwg

Description

The present invention relates to medicine, namely to herbal medicine, and can be used for the prevention and treatment of cerebrovascular accidents (NMC).

The State Register of Medicinal Products and Medical Devices includes more than 200 types of medicinal plants, of which only preparations of small periwinkle (vincamine, vinpocetin) and two-bladed ginkgo (ginkgo biloba, bilobil) are used to treat cerebrovascular disorders, their action is aimed at the disappearance of subjective symptoms diseases (improving cerebral circulation, hypotensive, antispasmodic, vasodilator, etc.) [State Register of Medicines / Ch. Editor R.U. Khabriev. - M.: Scientific Center for Expertise of Medical Devices of the Ministry of Health of Russia, 2004. - T.1. -1405 p.]. The prototype is a small periwinkle. However, the drugs used have a number of drawbacks: insufficient raw material base, the effect only on individual parts of NMC.

The objective of the invention is to expand the arsenal of herbal medicines of various types of pharmacological actions for the prevention and treatment of NMC.

The technical result achieved using the inventions is to obtain a plant collection with a pronounced therapeutic effect for the treatment of NMC.

The proposed collection for the prophylaxis and treatment of MMC contains vinca leaves, peppermint leaves, plantain leaves, hawthorn berries, rose hips, rowan fruits, common wormwood grass, sweet clover grass, dried cinnamon grass, chamomile flowers, licorice roots with a ratio of components in parts respectively 1: 1: 1: 1: 1: 1: 1: 2: 1: 1: 1.

The collection includes medicinal plants that are approved for use in medical practice [State Pharmacopoeia of the USSR, Eleventh Edition. Issue 2 - M, 1990, VFS 42-1728-87, FS 42-2094-83, FSP 42-0296-2339-02, FSP 42-0330168301].

The technology for preparing the collection is that each type of medicinal raw material that meets the requirements of the State Pharmacopoeia X and XI edition is crushed to a particle size of not more than 7 mm. Then the medicinal raw materials are weighed and thoroughly mixed in equal parts to a uniform mixture and filtered from dust through a sieve with a hole diameter of 0.18 mm. To prepare an aqueous extraction of 4.0 g of collection, 200 ml of purified room temperature water is poured, infused in an infundir apparatus for 15 minutes, then cooled at room temperature for 45 minutes, filtered, squeezing the plant material, and water is added to the required extraction volume .

The antihypoxic effect of the collection was studied on outbred mice to increase life expectancy in normobaric hypoxia.

The study of acute collection toxicity was carried out by introducing 10% infusion in doses of 1500-15000 mg / kg of animal weight (white mongrel mice of both sexes, weighing 18-20 g), calculated on the dry weight of the collection intraperitoneally and orally. The toxicity parameters were calculated by the method of probit analysis according to Litchfield-Wilcoxon.

The antiplatelet activity of the collection was determined by the modified Born method using an AT-02 platelet analyzer connected to a computer using a serial COM port with an ACOM interface and equipped with the original manufacturer AT-02.EXE program for recording the results.

The anticoagulant activity of the collection was determined by counting the clotting time of the prepared plasma before the formation of fibrin. Similarly, prothrombin time was determined in plasma samples with the studied collection.

The antioxidant activity of the test collection was judged by its ability to inhibit the autooxidation of adrenaline in vitro and thereby prevent the formation of reactive oxygen species (method 1) and the express method was used on cell culture by fixing the concentration causing lysis (method 2).

Figure 1 shows the effect of the proposed collection on platelet aggregation caused by ADP, collagen and adrenaline; figure 2 - the impact of the proposed collection on the factors of the prothrombin complex; figure 3 - comparative data of methods 1-2 antioxidant activity of the collection.

Example 1. The effect of collection for the prevention and treatment of NMC on hypoxia

Take a collection that contains: hawthorn fruits - 1 hour, rose hips - 1 hour, rowan fruits - 1 hour, common wormwood grass - 1 hour, peppermint leaves - 1 hour, leaves of small periwinkle - 1 hour. , the grass of the dried marshmallow - 1 hour, the leaves of the large plantain - 1 hour, the chamomile flowers - 1 hour, the licorice roots - 1 hour, the clover grass - 2 hours; 10 g of collection mixtures are poured with 100 ml of purified water and insisted for 15 minutes in an infundir apparatus, cooled at room temperature for 45 minutes. The collection was introduced intragastrically in the form of 10% freshly prepared infusion with the calculation of 1.5 ml / kg, 1 hour before the start of the experiment, taking into account the peak of its action. One animal was placed in hermetically sealed 500 ml jars. The animals were monitored until the cessation of breathing.

It was found that collection increases resistance to normobaric hypoxia, the death of animals decreases by 44%.

Example 2. Acute toxicity of collection

The collection was introduced in the form of 10% infusion in doses of 1500-15000 mg / kg of animal weight, calculated on the dry weight of the collection, intraperitoneally and orally. Animals were monitored for 14 days; noted the clinical picture of poisoning, established the relationship between the number of surviving animals and the dose.

According to the classification of Izmerov N.F. collection with intraperitoneal administration belong to class 5 - practically non-toxic substances (LD ₅₀ = 10050 (6281 ÷ 16080)).

Example 3. Antiplatelet activity of the collection

The aggregation process was induced by adding a standard amount of platelet aggregation agonist (ADP, collagen, adrenaline) to platelet-rich human plasma, and recorded photometrically by the decrease in its optical density (540 nm).

Thus, from the data presented it follows that the collection of NMC has antiplatelet activity, with platelet aggregation caused by ADP, collagen and adrenaline (figure 1).

Example 4. Anticoagulant collection activity

For the study used blood from the cubital vein, to which was added a 3.8% solution of sodium citrate (9: 1). Blood was centrifuged immediately after taking blood at 3000 rpm for 15 min, plasma was taken for research immediately after centrifugation.

When determining control parameters, 0.1 ml of control plasma was added to the test tube, incubated at a temperature of + 37 ° C for 1 min, 0.2 ml of diluted Tehplastin having a temperature of + 37 ° C was added, and the coagulation time started to form fibrin.

Similarly, prothrombin time (PV) was determined in plasma samples with the studied collection:

Based on the data obtained, the collection at NMC has a high anticoagulant activity (figure 2).

Example 5. Antioxidant activity of the collection

For the study, 0.1 ml of a 0.1% solution of adrenaline hydrochloride was added to 2 ml of bicarbonate buffer (pH = 10.65) and the optical density was determined after 20 minutes at a wavelength of 347 nm in a 10 mm thick cuvette using an SF-46 spectrophotometer ( OD ₁ ). Then, to 2 ml of bicarbonate buffer (pH = 10.65), 0.01 ml of the test infusion from plant material or compositions and 0.1 ml of 0.1% adrenaline hydrochloride were added and the optical density was determined after 20 minutes at a wavelength of 347 nm in a cuvette 10 mm thick on an SF-46 spectrophotometer (OD ₂ ).

Antioxidant activity (AOA) was calculated by the formula:

The collection, consisting of 11 types of medicinal plant materials, has the highest antioxidant activity (AOA - 77%).

Method 2 was used to confirm the antioxidant activity of the collection.

Infusoria of the species Parametium caudatum were cultured in laboratory conditions on a Lozin-Lozinsky medium containing 0.1 g of sodium chloride, 0.01 g of potassium chloride, calcium chloride, magnesium chloride and 0.02 g of sodium bicarbonate, at a temperature of 22-25 ° C , in natural light, avoiding direct sunlight. For infusoria nutrition, an infusion of oat grains (1-3 grains per 10 ml of culture) was used. Paramecium cultivation was carried out in 30-50 ml flasks. The height of the cultural layer is not more than 3 cm for free penetration of oxygen, the medium is neutral.

The test infusion was added to the Paramecium culture and kept in a chronic experiment for 24-72 hours, i.e. the period when protective mechanisms are formed, then a pathological model of damage to the paramecium membranes was created with a 14% solution of ethyl alcohol and a 3% solution of hydrogen peroxide, detecting the time of their complete stop. Further increasing the percentage of poisons, the concentration causing lysis was recorded. Both methods give objective results, according to which the studied recipe collection has a sufficiently high antioxidant activity (figure 3).

Thus, the proposed collection allows you to expand the arsenal of herbal medicines of multivalent action for the prevention and treatment of cerebrovascular diseases, as it has a pronounced anticoagulant, antiplatelet, antioxidant effect, increases resistance to normobaric hypoxia.

Claims (1)

Means for the prevention and treatment of cerebral circulation, containing the leaves of small periwinkle, characterized in that it additionally contains peppermint leaves, large plantain leaves, hawthorn fruits, rose hips, rowan fruits, common wormwood grass, sweet clover grass, clover grass, chamomile flowers , licorice roots with a ratio of components in parts respectively 1: 1: 1: 1: 1: 1: 1: 2: 1: 1: 1.

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