KR20130081852A - Composition for enhancing immune system containing selaginella tamariscina spring extracts or fractions thereof - Google Patents

Abstract

PURPOSE: A composition for immunity sterengthening is provided to strengthen immunity by having a Selaginellar tamariscina Spring extract or a fraction of a Selaginellar tamariscina Spring extract as an active ingredient. CONSTITUTION: A composition for immunity strengthening contains a Selaginellar tamariscina Spring extract or a fraction of a Selaginellar tamariscina Spring extract as an active ingredient. The composition contains a 0.02-50 weight% of the Selaginellar tamariscina Spring extract or the fraction of the Selaginellar tamariscina Spring extract per total weight of the composition. The Selaginellar tamariscina Spring extract is extracted using low quality alcohols ranged from C1 to C4 or a mixed solvent thereof. The low quality alcohols in with C1-C4 are methanol or ethanol. The Selaginellar tamariscina Spring extract fraction is fractionated using a non-polar or polar organic solvent. [Reference numerals] (AA) Leukocyte count; (BB,CC) Experimental group; (DD) Control group; (EE) Day seven; (FF) Day fourteen

Description

Composition for strengthening immunity including butcherson extract or fractions thereof {COMPOSITION FOR ENHANCING IMMUNE SYSTEM CONTAINING SELAGINELLA TAMARISCINA SPRING EXTRACTS OR FRACTIONS THEREOF}

The present invention relates to a composition for strengthening immunity, and more particularly, to a composition for enhancing immunity comprising Buddha extract or a fraction thereof as an active ingredient.

Immunity is a defense system that protects the body on its own against resistance to pathogens and viruses from the outside. When immunity is strong, the immune system is not only susceptible to disease, but also recovers in a short time even if the disease occurs.

However, this immunity can be easily lowered by various causes such as poor diet, obesity, lack of exercise, stress, smoking, drinking, lack of sleep, and the like. In other words, malnutrition caused by poor diet can inhibit the activity of immune cells, and stress can lead to imbalance of hormone secretion, thereby reducing immune function by reducing the number of lymphocytes. When the immunity is lowered like this, the possibility of developing an infectious disease such as a cold, an inflammatory disease, an allergic disease, chronic fatigue, or a cancer is rapidly increased, and therefore, special care is required to strengthen and maintain the immunity.

On the other hand, Selaginella tamariscina Spring is an evergreen perennial herb that grows in stone gaps in Jeju Island, Ulleungdo, South, Central, and North Korea. It is about 20cm tall and leaves are 1.5 ~ 2㎜ long. It is a projection and is densely arranged in 4 lines and ovate. The branches are split into planes, the surface is dark green, and the back side is white green. Branches spread in all directions when wet, and curled into balls when dry, and spread again when there is moisture. Spores are 0.5 ~ 1.5㎝ long, 2㎜ in diameter, one at the end of twigs, square. It is used for ornamental purposes, and leaves, stems, and roots are used for medicinal purposes.

As a result of the above, development of a composition for enhancing immunity has become an important problem, and it is of interest to use a buddhason as an active ingredient of such a composition. In addition, until now, the application for the Buddha's hand is mostly related to the cosmetic composition (Patent Application No. 10-2008-0097572) containing the Buddha's hand extract. It is incomplete.

The present invention has been proposed to solve the above problems of the conventionally proposed methods, by including the Buddha hand extract or fractions thereof as an active ingredient, an object of the present invention to provide a composition for enhancing immunity that can enhance immunity. .

According to a feature of the present invention for achieving the above object, the composition for enhancing immunity,

It is characterized by its composition comprising the Buddha extract or a fraction thereof as an active ingredient.

Preferably, the composition is

With respect to the total weight of the composition may include the fraction of the Buddha extract or the Buddha extract as 0.02 to 50% by weight.

Preferably, the Buddha hand extract,

Extraction may be performed using water, C1 to C4 lower alcohols, or a mixed solvent thereof.

More preferably, the lower alcohol of C1 to C4,

Methanol or ethanol.

Preferably, the fraction is,

The Buddha extract can be fractionated using a nonpolar or polar organic solvent.

More preferably, the nonpolar organic solvent,

Hexane, chloroform, methylene chloride or ethyl acetate.

More preferably, the polar organic solvent,

Acetone, ethanol, methanol or butanol.

According to the composition for enhancing immunity proposed in the present invention, by including the Buddha extract or a fraction thereof as an active ingredient, it is possible to enhance the immunity.

1 is a view showing the results of a leukocyte number change experiment according to an extract or a fraction of Busonson as an active ingredient in a composition for enhancing immunity according to an embodiment of the present invention.
Figure 2 is a view showing the results of the cortisol concentration change experiment according to the butcherson extract or fractions thereof as an active ingredient in the composition for enhancing immunity according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, in order that those skilled in the art can easily carry out the present invention. In the following detailed description of the preferred embodiments of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In the drawings, like reference numerals are used throughout the drawings.

In addition, in the entire specification, when a part is referred to as being 'connected' to another part, it may be referred to as 'indirectly connected' not only with 'directly connected' . In addition, the term 'comprising' of an element means that the element may further include other elements, not to exclude other elements unless specifically stated otherwise.

In the present invention, the term "active ingredient" refers to a substance or a group of substances (a pharmacologically active ingredient or the like, which is expected to express the efficacy or effect of the composition directly or indirectly by intrinsic pharmacological action). It means containing a main component).

Immunity enhancing composition according to an embodiment of the present invention, characterized in that it comprises Buddha extract or a fraction thereof as an active ingredient. More specifically, the Butcherson extract can be extracted using water, a lower alcohol of C1 to C4, or a mixed solvent thereof, and the fraction can be obtained by adding an organic solvent to the Butcherson extract. In addition, the content of the Buddha extract or fractions thereof contained in the composition for enhancing immunity according to an embodiment of the present invention is preferably included in 0.02 ~ 50% by weight relative to the total weight% of the composition. When included in less than 0.02% by weight, almost no action effect of the Buddha extract or fractions thereof, and exceeds 50% by weight is not only disadvantageous to the stability of the composition, but also does not show a synergistic effect of the effect due to excess addition is not preferred. .

Hereinafter, the process of preparing the Buddha extract or fractions thereof in the composition for enhancing immunity according to an embodiment of the present invention will be described in detail.

Butcherson extract can be extracted using water, C1 to C4 lower alcohol or a mixed solvent thereof. That is, the leaves, stems, and roots of the Buddha's hand are cut into whole or small pieces or pulverized by a pulverizer, and then finely powdered, and then put into an extraction container, followed by water, a lower alcohol of C1 to C4, preferably methanol or ethanol, or these. After mixing and extracting a mixed solvent of, it was left for a certain time and then filtered through a filter paper, etc. to obtain the Buddha hand extract. On the other hand, the method of extracting the extract from the Buddha hand can be extracted through various methods such as hot water extraction, cold needle extraction, reflux extraction, ultrasonic extraction, but is not limited thereto.

In addition, the fraction of the Buddha extract can be obtained by suspending the Buddha extract in water or distilled water and then fractionating by adding a nonpolar or polar organic solvent. Nonpolar organic solvents may include hexane, chloroform, methylene chloride or ethyl acetate, and polar organic solvents may include but are not limited to acetone, butanol, ethanol or methanol.

The present inventors confirmed the effect of the Buddha extract or fractions thereof obtained as described above on the leukocyte count, and as a result, the Buddha extract or fractions thereof according to the present invention showed an excellent effect of enhancing immunity by increasing the number of leukocytes. (See Table 1 and FIG. 1).

In addition, the present inventors also confirmed the effect of the Buddha extract or fractions thereof obtained as described above on the change in the amount of cortisol, as a result, the Buddha extract or fractions thereof according to the present invention is effective in strengthening immunity by inhibiting the secretion of cortisol Excellent was confirmed (see Table 2 and FIG. 2).

On the other hand, the composition according to the present invention may be provided as a pharmaceutical composition. When the composition according to the present invention is prepared as a pharmaceutical composition, the composition according to the present invention is not limited thereto, but is used to prevent and treat diseases caused by lowering immunity such as infectious diseases such as colds, inflammatory diseases, allergic diseases, etc. It may be provided in a composition. In addition, when the composition according to the present invention is prepared as a pharmaceutical composition, the composition according to the present invention may further include a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers include, but are not limited to, physiological saline, polyethylene glycol, ethanol, vegetable oils and isopropyl myristate, as commonly used in the formulation.

Pharmaceutical dosage forms of the composition for enhancing immunity according to one embodiment of the present invention may be used in the form of their pharmaceutically acceptable salts, and may also be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection. have.

In addition, the composition for enhancing immunity according to the embodiment of the present invention may be used in the form of oral formulations, suppositories, and sterile injectable solutions, such as powders, tablets, capsules, granules, syrups, and aerosols, respectively, according to a conventional method. Can be. When formulated, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, surfactants, etc. which are commonly used. Oral formulations may be prepared by mixing one or more excipients such as starch, calcium carbonate, sucrose, lactose, mannitol, and the like, with the Bucherson extract or fractions thereof as an active ingredient. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like can also be used. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspending agents, and the like, and non-aqueous solvents and suspending agents may include propylene glycol, vegetable oils such as polyethylene glycol, and injectable esters such as ethyl oleate.

Preferred dosages of the composition for enhancing immunity according to one embodiment of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the drug form, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, preferably, the pharmaceutical composition according to one embodiment of the present invention is administered at 0.001-1000 mg / kg body weight per day, more preferably 0.01-500 mg / kg body weight. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The pharmaceutical composition according to one embodiment of the present invention may be administered to mammals such as rats, mice, livestock, humans by various routes. The method of administration is not limited and may be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine epidural or cerebrovascular (intracerbroventricular) injection.

The present invention is explained in more detail by the following examples, but the present invention is not limited in any way by the following examples.

Buddha hand extract manufacturer

The dried Buddhas (collected from Jeju, South Korea) were chopped and placed in an extraction container. Subsequently, the mixture was immersed in 100% methanol having a volume of 10 times the weight of the budsonson, extracted under reflux for 10 hours, cooled at room temperature, and filtered through a filter paper. The extract was concentrated under reduced pressure using a concentrator until the solution was completely evaporated, thereby obtaining Buddha extract (83 g).

Of Buddha Hand Extract Fraction  Produce

The Busonson extract (83 g) obtained in Example 1 was suspended in distilled water (1 L), and then fractionated using hexane, chloroform, ethyl acetate, and butanol, and extracted with hexane (4.9 g), chloroform (11.3 g), and ethyl acetate (12 g), respectively. , Butanol (15.2 g) and aqueous (13.8 g) fractions were obtained.

Hereinafter, the effects of the present invention will be described in more detail through experimental examples, but the scope of the present invention is not limited by the following experimental examples.

[Experimental Example 1]

White blood cell count experiment

In order to confirm the immune-enhancing effect by the immunity-enhancing composition comprising the Buddha extract or fractions thereof according to the present invention, the animal experiment was carried out as follows.

1. Subject and Period

The subjects of this experiment were 100-200 g 6-week-old rats (SD rats), and the experimental period was 2 weeks.

2. Experimental method

After dividing the experimental rats (SD rats) into two experimental groups and one control group of five animals each, 30 mg / kg of the Buddha extract prepared in Example 1 in Experimental group 1, and the fraction (ethyl acetate) prepared in Example 2 in Experimental group 2 Fraction)) 30 mg / kg, saline 30 mg / kg in the control group using a syringe for oral administration, blood was collected through cardiac puncture at 7 and 14 days once daily to give a white blood cell count. Was measured.

3. Results

The number of leukocytes in blood of experimental group 1, experimental group 2 and control group was measured, and the results are shown in Table 1 and FIG. 1. On the other hand, the leukocyte counts shown in Table 1 and Fig. 1 represent the average of five mice belonging to each group.

White blood cell count (cell / μl) 7 days 14 days Experiment 1 6,897 ± 3,256 8,234 ± 3,012 Experiment 2 6,648 ± 2,549 7,945 ± 2,754 Control group 5,712 ± 2,512 5,814 ± 1,865

As shown in Table 1 and Figure 1, compared with the control group, it was confirmed that the number of leukocytes increased in the experimental group 1 and the experimental group 2. Through this, the Buddha hand extract or fractions thereof according to the present invention was found to be excellent immunity enhancing effect.

[Experimental Example 2]

Cortisol Concentration Measurement Experiment

In order to confirm the immune-enhancing effect by the immunity-enhancing composition comprising the Buddha extract or fractions thereof according to the present invention, the animal experiment was carried out as follows.

1. Subject and Period

The subjects of this experiment were 100-200 g 6-week-old rats (SD rats), and the experimental period was 3 days.

2. Experimental method

After dividing the experimental rats (SD rats) into two experimental groups, each of 5 rats, and one control group and a normal group, the water of 30 cm in height and 30 cm in width was filled with water at 25 ° C., and SD of the other groups except the normal group. Each rat was placed in a tank and forced to swim for 20 minutes. Thereafter, using the oral syringe, Experimental group 1, 20mg / kg of Busonson extract prepared in Example 1, Experimental group 2, 20mg / kg of fraction (particularly ethyl acetate fraction) prepared in Example 2, control group Was administered saline 20mg / ㎏, and the normal group did not receive any treatment. After 1 hour of administration, blood was collected from the heart of all groups of SD rats to measure the amount of cortisol, which was repeated three times.

3. Results

The amount of cortisol in blood of the experimental group 1, the experimental group 2, the control group and the normal group was measured, and the results are shown in Table 2 and FIG. 2. In addition, the quantity of the cortisol shown in Table 2 and FIG. 2 shows the average value measured by repeating 3 times.

division Cortisol (㎍ / ㎗) Experiment 1 24.2 Experiment 2 23.8 Control group 48.4 Normal group 10.2

As shown in Table 2 and FIG. 2, the cortisol was excessively secreted due to forced swimming in the control group compared to the normal group, but in the experimental group 1 and the experimental group 2, the secretion of the cortisol was suppressed, and thus the difference between the control group and the cortisol was significantly different. Indicated. Excessive secretion of cortisol not only inhibits the release of transporters such as interleukin and interferon, but also reduces the number of lymphocytes through various mechanisms, leading to the death of lymphocytes. Therefore, the Buddha extract or fractions thereof according to the present invention inhibits the secretion of cortisol and prevents the reduction of lymphocytes.

[Experimental Example 3]

Toxicity test

Experimental rats (SD rats) were divided into two experimental groups, each of three, each of which was divided into two experimental groups. In the experimental group 1, the Busonson extract prepared in Example 1, and in the experimental group 2, the fractions prepared in Example 2 (particularly, ethyl acetate fraction) were each 20 mg / No toxic effects were observed and no death was observed after 7 days of injecting kg, 10 mg / kg or 1 mg / kg. Therefore, it was confirmed that the Buddha extract or fractions thereof according to the present invention is a safe drug.

In the following, the preparation examples of the composition of the present invention comprising a butcherson extract or a fraction thereof as an active ingredient, but the scope of the present invention is not limited by the following preparation examples.

[Example 1]

Sanje  Produce

Table 3 shows a preparation example according to an embodiment of the present invention, in the form of a powder.

ingredient content Buddha hand extract of Example 1 or fraction of Example 2 25mg Lactose 100 mg Talc 5mg

The above components are mixed and filled in airtight bags to prepare powders.

[Example 2]

Manufacture of tablets

Table 4 shows an example of the preparation according to an embodiment of the present invention, the formulation of the tablet form.

ingredient content Buddha hand extract of Example 1 or fraction of Example 2 20 mg Lactose 100 mg Corn starch 100 mg Magnesium stearate 0.2 mg

The tablets are prepared by mixing the components and then tableting according to a conventional method for preparing tablets.

[Example 3]

Preparation of capsules

Table 5 shows a preparation example according to an embodiment of the present invention, a capsule form preparation example.

ingredient content Buddha hand extract of Example 1 or fraction of Example 2 10 mg Crystalline cellulose 2 mg Lactose 20 mg Talc 0.2 mg Magnesium stearate 0.2 mg

According to a conventional capsule manufacturing method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

[Example 4]

Liquid  Produce

Table 6 shows a preparation example according to an embodiment of the present invention, in the form of a liquid.

ingredient content Buddha hand extract of Example 1 or fraction of Example 2 30mg Mannitol 5g Isomer 10g Purified water Suitable amount

According to the conventional method of preparing a liquid solution, each component is added to the purified water to dissolve it, and then the lemon flavor is appropriately mixed, the above components are mixed, and then purified water is added to adjust the total amount to 100 ml. Manufacture.

The present invention may be embodied in many other specific forms without departing from the spirit or essential characteristics of the invention.

Claims (7)

Butcherson extract or a fraction thereof as an active ingredient, characterized in that for enhancing the immune system.
2. The composition of claim 1,
To the total weight of the composition characterized in that it comprises 0.02 ~ 50% by weight of the Buddha extract or the fraction of the Buddha extract, composition for strengthening immunity.
According to claim 1, wherein the Buddha hand extract,
Immunity enhancing composition, characterized in that the extraction using water, C1 to C4 lower alcohol or a mixed solvent thereof.
According to claim 3, wherein the lower alcohol of C1 to C4,
Methanol or ethanol, characterized in that the composition for enhancing immunity.
The method of claim 1, wherein the fraction,
Characterized in that the Buddha extract extract using a non-polar or polar organic solvent, immunity enhancing composition.
The method of claim 5, wherein the nonpolar organic solvent,
Hexane, chloroform, methylene chloride or ethyl acetate, characterized in that the composition for enhancing immunity.
The method of claim 5, wherein the polar organic solvent,
Acetone, ethanol, methanol or butanol, characterized in that the composition for enhancing immunity.

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