RU2566656C1 - Medium for dilution, long-term storage and conservation of semen of turkeys "vniigrzh-5" - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: medium comprises sodium glutamate, glutamic acid, inositol, potassium phosphate dibasic, glucose and magnesium sulphate dissolved in distilled water. All the components are taken in a specific ratio. And in the cryopreservation of semen the medium additionally comprises N,N-dimethylacetamide at a concentration of 8%.

EFFECT: use of invention enables to improve the biological full-value of sperm cells of turkeys during long-term storage and cryopreservation.

2 cl, 3 tbl

Description

The invention relates to poultry and can be used in biotechnologies for artificial insemination of turkeys.

With the development of artificial insemination methods for farm poultry, the development of media for dilution, long-term storage and cryopreservation of male sperm in order to increase egg fertilization has become urgent. Moreover, the composition of the media is different for different types of birds.

One of the tasks is the long-term storage and cryopreservation of turkey semen. To solve it, among others, a medium was proposed containing dissolved in distilled water: inositol, lactose, potassium phosphate disubstituted and monosubstituted, sodium thiosulfate and zinc complex of disodium dihydrogen ethylene diamine tetraacetate [1]. The main disadvantage of this medium is the relatively low rates of egg fertilization (63.3%) and young hatching (49.9%) obtained after insemination with sperm that underwent cryoconservation.

As a prototype, the VIRGZH-4 medium was chosen, having the composition of components closest to the claimed object of the invention [2], used for cryopreservation of semen of roosters and turkeys.

The objective of the invention was to significantly increase the biological usefulness of turkey sperm during prolonged storage at positive temperatures and cryopreservation and, as a result, increase the fertility of eggs.

According to the results of numerous experiments, it was found that to solve this problem, it is advisable to use sulfate as the sources of magnesium ions in the prototype of the acetate and bring the ratio of components dissolved in 100 ml of distilled water to the following ranges: monosodium glutamate - 1.60-2.00 g; glutamic acid - 0.04-0.05 g; inositol - 0.24-0.26 g; potassium phosphate disubstituted - 0.32-0.36 g; glucose - 1.00-1.14 g; magnesium sulfate 0.22-0.23.

When using this medium for cryopreservation of sperm, turkeys as a cryoprotectant add 8% N, N-dimethylacetamide to the volume of diluted sperm.

To test the ranges of the content of components in comparison with the prototype, 4 variants of the new medium, called “VNIIRGZH-5”, the compositions of which are shown in table 1, were tested.

Dilution rate: 1 part sperm and 3 parts medium. The frequency of insemination of turkeys during storage of sperm at positive temperatures - 1 time in 14 days; when using frozen-thawed sperm - 1 time in 7 days.

Table 2 shows the results of comparative tests of various variants of the medium after freezing, storage for a year at a temperature of liquid nitrogen and subsequent thawing, which shows that the object of the invention has a significant advantage over the prototype, especially noticeable in options II and III.

Table 3 shows the results of comparative tests of the prototype and the composition of the proposed medium according to option III (both without N, N-dimethylacetamide) for different storage periods at a temperature of + 1 ... + 3 ° C. There is also an advantage of the subject matter.

The VNIIGRZh-5 medium is prepared as follows: 50 ml of distilled water are poured into the first container, in which they dissolve: monosodium glutamate and disubstituted potassium phosphate. In a second container, glucose, inositol, magnesium sulfate and glutamic acid are dissolved in 50 ml of distilled water. After complete dissolution of the components, both solutions are combined in one container.

The technical result of the invention consists in a significant increase in the biological usefulness of turkey sperm during prolonged storage and cryopreservation.

Table 1 Environment components Units measuring Wednesday "VNIIGRZH-5" Prototype "VIRGZH-4" Option I Option II Option III Option IV Monosodium glutamate g 1.26 1,60 2.00 2.40 2.40 Glutamic acid g 0,03 0.04 0.05 0.06 0.14 Inositol g 0.23 0.24 0.26 0.30 0.20 Potassium Disubstituted Potassium Phosphate g 0.29 0.32 0.36 0.40 0.35 Glucose g 0.87 1.00 1.14 1.35 0.80 Magnesium sulfate g 0.20 0.22 0.23 0.25 - Magnesium acetate g - - - - 0.10 Glycine g - - - - 0.10 N, N-dimethylacetamide % 8.00 8.00 8.00 8.00 8.00 Distilled water ml one hundred one hundred one hundred one hundred one hundred

table 2 Wednesday Options Incubated eggs, pcs. Egg Fertilization,% The output of young animals,% Object of invention "VNIIGRZH-5" I 200 67.2 60.0 II 213 81.2 72.5 III 198 86.9 79.6 IV 385 70,2 61.3 Prototype "VIRGZH-4" 328 79.1 72.9

Table 3 Wednesday Sperm storage time, hour Incubated eggs, pcs. Egg Fertilization,% The output of young animals,% The object of the invention "VNIIGRZH-5", option III one 1378 97.0 83.8 24 831 95.2 82.8 48 284 89.1 71.1 Prototype "VIRGZH-4" 24 1324 85.6 73.0

Information sources

1. Ostashko F.I., Besulin V.I., Karkach P.M. Medium for dilution and freezing of turkey semen: Auth. St. USSR No. 1477397, A61D 7/02, 1987.

2. Tselyutin K.V., Tour B.K. Artificial insemination and cryopreservation of sperm of poultry (roosters, turkeys, gander and drakes). - St. Petersburg - Pushkin. - 2013 .-- S. 29.

Claims (2)

1. Medium for dilution and long-term storage of turkey semen, characterized in that it contains sodium glutamate, glutamic acid, inositol, potassium phosphate disubstituted, glucose and magnesium sulfate dissolved in distilled water in the following ratio of components:
Monosodium glutamate - 1.60-2.00 g
Glutamic acid - 0.04-0.05 g
Inositol - 0.24-0.26 g
Disubstituted potassium phosphate - 0.32-0.36 g
Glucose - 1.00-1.14 g
Magnesium sulfate - 0.22-0.23 g
Distilled water - 100 ml 2. Medium for dilution and long-term storage of turkey sperm according to claim 1, characterized in that when the sperm are cryopreserved, it additionally contains 8% N, N-dimethylacetamide.