RU2548767C1 - Method for producing befungin preparation of chaga muschroom - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method for producing Befungin, a preparation of Chaga mushroom involving extracting the milled raw material in heated water on a heating unit consisting of 3 reactors; adding cobalt salts to the extract in certain environment, mixing with ethanol, evaporating to dry residue of 20-25%.

EFFECT: method enables accelerating the production process, reducing energy demands, improving the quality and completeness of extraction, and improving the quality of the preparation.

2 dwg, 1 tbl, 1 ex

Description

The invention relates to the production of medicines and can be used to obtain medicines Befungin from birch fungus chaga, formed on birches by the phytopathogenic parasite Inonotus obliquus. The drug has a tonic, tonic, antioxidant, immunomodulatory, anti-inflammatory effect. It normalizes the metabolism and activity of the gastrointestinal tract, is a symptomatic tool that improves the general condition of cancer patients. It is used for diseases of the gastrointestinal tract (hypoacid gastritis, peptic ulcer, polyposis of the stomach and intestines, biliary tract and intestines hypokinesia), as well as for skin diseases, periodontal disease and as a symptomatic agent for oncological diseases.

A known method, including obtaining diffusion juice of chaga, its treatment with an aqueous solution of hyperbranched polymer with a concentration of 1 · 10 ^-7 -1 · 10 ^-20 % in a ratio of 10: 1 at room temperature for 0.5-1.5 hours, the deposition of polyphenol complex with a 20% hydrochloric acid solution to a pH of 2.0-2.2 to obtain a precipitate of a polyphenolic complex, moreover, Boltorn 40 is used as a hyperbranched polymer [RU 2425686, C1, A61K 36/06, 08/10/2011].

The disadvantage of this method is the relatively high complexity.

In addition, there is a method for producing a precipitated preparation from birch fungus fungus by producing chaga diffusion juice, precipitating it, adding a 20% hydrochloric acid solution to a pH of 2.0-2.2, and adding aqueous to the chaga diffusion juice a solution of hyperbranched polymer with a concentration of 1 · 10 ^-7 -10 ^-20 % in a ratio of 10: 1, processing is carried out at room temperature for 0.5-1.5 hours [RU 2366439, C1, A61K 36/06, 09/10/2009 ].

The disadvantage of this method is the relatively high complexity.

There is also a method of producing chaga powder, including grinding the raw material in an inert liquefied gas medium, while freshly prepared raw materials are pre-treated with an inert liquefied gas for 30-60 minutes, then dried by vacuum sublimation to a residual moisture content of 1-8%, moreover, the ratio of raw materials and inert liquefied gas during the preliminary processing of freshly prepared raw materials and when grinding it in an inert liquefied gas environment take (1: 1) - (1: 3) [RU 2167665, C1, A61K 35/78, 05.27.2001].

This method is also characterized by relatively high complexity.

Closest to the proposed prototype is a method of obtaining a preparation from chaga birch mushroom, including extraction of crushed chaga grains heated up with water (up to 2-3 mm), adding cobalt salts to the extract, boiling up to 32-33% of the dry residue, mixing with rectified alcohol to obtaining a thick extract and further drying the resulting mixture [SU 104607, A1, A61K 35/78, 01/01/1956].

The disadvantage of this method is the relatively low quality of the resulting drug, since it does not provide modes for the individual stages of the method, providing increased extraction.

Grinding chaga to a size of 2-3 mm during extraction leads to leaching of a large amount of ballast substances into the extractant and difficulties in filtering.

Boiling the extract to 32-33% of the dry residue has its drawbacks:

- an increase in the duration of production, an increase in energy consumption;

- difficulty unloading from the reactor, as well as losses - subsidence on the walls of the reactor;

- increase the duration of the standardization process;

- difficulties in packing the drug;

- inconvenience of using the consumer, because a thick mass is difficult to dose.

The required technical result is a significant acceleration of the production process; reduction of energy costs per unit of production; improving the quality of extraction; achieving complete extraction; increase in production volumes; improving the quality of the drug.

The required technical result is achieved by the fact that in the method for preparing Befungin from chaga birch mushroom, including the extraction of ground raw material with water, adding cobalt salts to the extract, evaporation and mixing with ethyl alcohol, and grinding of the starting material to particles with sizes from 7 to 15 mm, the extraction of active substances from chaga birch fungus is carried out on a battery of 3 reactors by mixing the crushed feedstock with the extractant in a mass ratio of 1:10 and keeping for 1.5 hours in the reactors at a temperature of 68-72 ° C, after which the finished hood is poured into a collector, cobalt salt is added to the hood, followed by evaporation of the hood at a temperature of 70-80 ° C under a vacuum of 0.06 MPa to dry residue 20-25%.

In addition, the required technical result is achieved by the fact that extractor is supplied to each battery reactor 3 times, then the depleted raw materials - chaga, are replaced with fresh ones.

In addition, the desired technical result is achieved by the fact that the last two extraction before draining the finished hood is made on fresh raw materials, after which the hood is poured into the collection.

Using the above extraction scheme (see Fig. 1 and Fig. 2) and evaporating the hood at a temperature of 70-80 ° C under a vacuum of 0.06 MPa can significantly accelerate the production of Befungin (up to 20% of the time to obtain 1 batch of the finished product) .

Evaporation of the extract to a dry residue of 20-25% allows to reduce energy costs for production up to 10% and to reduce losses during unloading of the extract from the reactor, to facilitate packing and dosing of the drug.

Grinding chaga to optimal particle sizes from 7 to 15 mm (selected experimentally) allows to improve the extraction process and reduce the leaching of ballast substances, which increases the quality of the product.

The optimal ratio of 1:10 raw materials - chaga and extractant allows you to maximize the use of raw materials.

The optimal temperature range of 68-72 ° C during the extraction process allows to achieve an improvement in the extraction of the active substance (polyphenol complex) from chaga up to 30%.

An example of the implementation of the proposed method, which is used to obtain the drug Befungin.

Chaga birch fungus is ground to a particle size of 7 to 15 mm.

Extraction of the extract of birch fungus chaga is carried out by mixing the ground mushroom chaga with purified water.

Extraction of the active substances from the birch fungus Chaga is carried out on a battery of 3 reactors at a temperature of 68 to 72 ° C, and heating is carried out by contact method with hot steam or non-contact method with dead steam using a shirt.

Chilled chaga mushroom is charged into reactor P.1 and purified water is added. Insist birch fungus chaga in the reactor P.1 for 1.5 hours at a temperature of 68 to 72 ° C. Shredded chaga mushroom is loaded into the reactor P.2 and the extract from the reactor P.1 is moved. Water is again poured into the reactor P.1. The contents of the reactors are heated from 68 to 72 ° C. After 1.5 hours, the first batch of the finished extract is poured from the reactor P.2 into the collector.

The hood from reactor P.1 is moved to reactor P.2. Water is again poured into the reactor P.1.

Extraction is carried out in reactors P.1 and P.2 at a temperature of 68 to 72 ° C for 1.5 hours. The chopped chaga mushroom is loaded into the reactor P.3 and the extract from the reactor P.2 is moved. After and before carrying out the shifts and before draining in the reactors, circulation (mixing) is performed. The meal from the reactor P.1 is removed, the reactor is washed and reloaded with chopped mushroom chaga. Extracts are drawn from reactor P.3 to reactor P.1, from reactor P.2 to reactor P.3. Extraction is carried out in reactors P.1 and P.3 at a temperature of 68 to 72 ° C. After 1.5 hours, the second batch of the finished extract is poured into the collection from the reactor P.1. An extractant is supplied 3 times to each battery reactor. At the beginning of the operating cycle, water is poured into the reactor P.1 3 times. The last two extraction before draining the finished hood is carried out on fresh raw materials, after which the hood is poured into the collection (see Fig. 1 and Fig. 2).

The next stage is the addition of cobalt salt to the extract based on the density of the extract. A sample of the hood is taken, the density is determined, and the required amount of cobalt salt is found.

Example

At a temperature of 20 ° C and an extract density of 1.038 g / cm ^{3, the} required amount of cobalt chloride will be 0.84 g per 1 liter of extract, and cobalt sulfate - 0.996 g per 1 liter.

Cobalt salts are dissolved in a small amount of extract in an intermediate tank, after which the resulting solution is poured into a collection tank.

Then carry out the stage of evaporation to obtain a liquid extract with a solids content of 20-25%. Evaporation is carried out under a vacuum of 0.06 MPa with a working stirrer and a temperature of from 70 to 80 ° C. The liquid extract is discharged from the reactor into an intermediate tank.

After the analysis of the liquid extract, ethyl alcohol and, if necessary, purified water are added to the extract to obtain a preparation with a solids content of 15-20%. The amount of ethyl alcohol and purified water is determined by the necessary calculations based on the results of the analysis.

Calculation Example

We have a sample of the extract weighing 380 kg and with a mass fraction of solids of 22%. It is necessary to calculate the amount of purified water and ethyl alcohol required to obtain a mass fraction of solids of 19.8%.

1. Determine the mass of Befungin after mixing:

2. Determine the mass of ethyl alcohol 96.3% (vol.d.).

We accept that the final concentration of ethyl alcohol in Befungin will be 11% (obd):

, где

where

M ₂ - Befungin mass after standardization;

K is the ethyl alcohol content in liters per 1 kg of its solution, depending on the concentration of alcohol used, according to the table for determining the ethyl alcohol content in water-alcohol solutions (see Table 1):

, где

where

0,112 - the volume of alcohol in liters per 1 kg of 11% solution,

1.1961 - the volume of alcohol in liters per 1 kg of a 96.3% solution.

3. Determine the mass of purified water:

422.22-380-39.54 = 2.68 kg

According to the calculation, the liquid extract is mixed with ethyl alcohol and purified water.

After receiving a positive analysis result, the obtained Befungin is poured from the reactor by gravity into intermediate containers with filtration through a filter fungus. A label is indicated on the bottle with filtered Befungin, indicating the name of the preparation, batch number, weight and transferred to the packaging area.

Claims (1)

A method of producing a Befungin preparation from chaga birch fungus, including extraction of ground raw material with heated water, adding cobalt salts to the extract and mixing with ethyl alcohol, characterized in that the starting material is ground to particles with sizes from 7 to 15 mm, extraction of active substances from Chaga birch fungus is carried out on a battery of 3 reactors by mixing the crushed feedstock with the extractant in a mass ratio of 1:10 and keeping for 1.5 hours in the reactors at a temperature of D 68-72 ° C, wherein in each reactor is fed with battery extractant 3 times and the two last extraction hoods finished before draining occur in the fresh feed, whereupon the finished extract is drained to the collection; add cobalt salts to the extract and evaporate at a temperature of 70-80 ° C under a vacuum of 0.06 MPa to a dry residue of 20-25%.

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