RU2547792C1 - Method of producing dry anatomical preparation of heart - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: in order to produce the dry anatomical preparation of the heart applied is a disembowelled non-opened heart, prepared by the removal of the epicardium and subepicardial cellular tissue. Successive defatting, drying and dehydration of the organ with its following colouring are carried out. To carry out deffating the organ is placed for 3 days at room temperature into composition No 1, which consists of acetone and white spirit, taken in equal parts. The volume of composition No 1 must exceed the heart volume by 5-6 times. Then, after carrying out drying, a cavity of the deffated heart is filled with cotton or small pieces of cotton fabric. For the following dehydration the organ is placed at room temperature for 7 days in composition No 2, which represents a solution of polyacrylate varnish in acetone, taken in a volume ratio of 5:1. After that, the organ is hung also at room temperature until its dries completely. The preparation production is finished by colouring anatomical structures with oil paints, diluted with polyacrylate varnish.

EFFECT: method simplifies the production of the anatomical preparation, excluding the stage of the preparation soaking with silicate glue or liquid potassium glass, with the exclusion of application of ethylene alcohol and phenol.

2 dwg

Description

The invention relates to human anatomy, can be used in pathological anatomy and comparative anatomy of humans and animals.

Methods for the manufacture of dry anatomical preparations of internal organs, including and hearts, are known from a number of sources, namely: MG Prives Methods of preserving anatomical preparations. - Medgiz L.O., 1956; Yaroslavtsev B.M. Anatomical technique (guidelines for the manufacture of anatomical and biological preparations) - Frunze, [b. and.], 1961; Gaivoronsky I.V., Starchik D.A., Grigoryan S.P., Nichiporuk G.I. New methods of embalming biological objects // Scientific reports. Ed. Belgorod University, 2000, No. 2. - S.31-32; Hagens G., Tiedemann K., Kriz W. The current potential of plastination. Anat Embryol., 1986, Vol. 175, p. 411-421.

As a prototype - the closest analogue - we can note one of the methods of manufacturing the drug described in the manual: Pikalyuk B.C., Moroz GA, Kutya S.A. Toolkit for the manufacture of anatomical preparations. - Simferopol, 2004 .-- pp. 43-44.

In this case, the heart taken from the corpse is dissected, fixed in a 10% formalin solution or saline solutions based on it, then degreased in a sealed container in gasoline for 2-4 days, dehydrated in a mixture of glycerol and 96 ° ethanol with phenol added, gradually changing the ratio of "glycerol: ethanol" from 1: 1 to 1: 6 for 6 days. After that, the drug is dried until the liquid has stopped draining, tinted and dipped for 3 days in liquid silicate glue or liquid potassium glass for impregnation; the remnants of water glass are removed with tampons, the preparation is again dried in air.

The disadvantages of the above method are that the method involves the step of processing with silicate glue or liquid potassium glass, as well as the use of ethanol in the manufacture of the preparation, the circulation of which requires strict reporting, and carbolic acid (phenol), which has an irritating effect.

The patented method is aimed at obtaining objective knowledge in the fields of basic science, studying options and patterns of the structure and development of the heart of humans and animals in normal and pathological processes.

The technical result, which the patented method is aimed at, consists in simplifying the manufacture of the anatomical preparation, eliminating the step of impregnating the preparation with silicate glue or liquid potassium glass, and also refusing to use ethyl alcohol and phenol.

The specified technical result is achieved due to the fact that the eviscent and prepared, but not open heart for 3 days is placed in the composition (solution) No. 1, consisting of acetone and white spirit, taken in equal parts, and the volume of the composition (solution) No. 1 should exceed the heart volume by 5-6 times, then the fat-free heart cavity is filled with cotton, the organ is placed for 7 days in the composition (solution) No. 2, which is a solution of polyacrylate varnish in acetone, taken in a volume ratio of 5: 1, after which the organ is suspended at room second temperature until the complete drying and fabrication of the drug coloring anatomical structures oil paints, lacquer diluted polyacrylate.

The essence of the patented method is that the heart is sequentially placed in a mixture of white spirit with acetone in a ratio of 1: 1 and then in a solution of polyacrylate varnish in acetone in a ratio of 5: 1, which eliminates the use of ethanol and phenol, after which the drug is stained oil paints based on polyacrylate varnish, which eliminates the stage of processing the drug with silicate glue or liquid potassium glass.

The method is illustrated by photographs of the finished anatomical preparation of the heart.

Figure 1 shows the preparation of the heart with the prepared right atrium 1, the right ventricle 2 and the pulmonary trunk 3 coming out of it, the left ventricle 4 and the aorta 5 coming out of it, and also with the right 6 and left 7 coronary arteries;

Figure 2 shows the preparation of the heart with removed coronary arteries.

The method is as follows.

The heart is removed from the chest cavity (eviscerated) and fixed in a 5% formalin solution for 2-3 days, after which the heart is dissected, that is, mechanically, using a scalpel and tweezers, the epicardium and subepicardial tissue are removed. For degreasing, the prepared heart is placed in the composition (solution) No. 1, which is a mixture of acetone and white spirit, taken in equal parts. The volume of solution No. 1 should be 5-6 times the volume of the drug. The drug is kept in solution No. 1 for 3 days at room temperature, changing the position of the drug in the container several times a day for its best soaking in the solution. The heart is removed from the composition (solution) No. 1, left under good ventilation until the liquid evaporates from the surface of the heart, after which the atria through the openings of the veins, and the ventricles through the openings of the pulmonary trunk and aorta are filled with cotton or small pieces of cotton fabric for Prevent organ deformation. Next, the organ for dehydration for 7 days at room temperature is placed in composition No. 2, which is a solution of a polyacrylate varnish, for example, Tsapon varnish, in acetone, taken in a volume ratio of 5: 1. After 7 days, the heart is removed from the composition (solution) No. 2 and suspended (depending on the mass of the organ) on a thread or in a hammock made of gauze under good ventilation at room temperature until completely dried (depending on the thickness of the organ wall, the drying time is usually 2-3 days). If necessary, a dried heart is cut in the right plane to demonstrate intracardiac anatomical structures or left unopened. The anatomical structures of interest are stained with oil paints diluted with polyacrylate varnish.

Thus, the use of a patented method of manufacturing a dry anatomical preparation of the heart and compositions (solutions) used for its implementation allows you to abandon the stage of impregnation of the preparation with silicate glue or liquid potassium glass, the use of ethyl alcohol and phenol, and thereby simplify the manufacture of the anatomical preparation.

Claims (1)

A method of manufacturing a dry anatomical preparation of the heart by successive degreasing, drying and dehydration of the organ, followed by its coloring, characterized in that the eviscured unopened heart, prepared by removing the epicardium and subepicardial fiber, is placed at room temperature in composition No. 1, consisting of degreasing for 3 days from acetone and white spirit, taken in equal parts, and the volume of composition No. 1 should exceed the volume of the heart by 5-6 times, then, after drying, the cavity of a fat-free heart is filled with cotton or small pieces of cotton cloth and for subsequent dehydration, the organ for 7 days is placed at room temperature in composition No. 2, which is a solution of polyacrylate varnish in acetone taken in a volume ratio of 5: 1, after which the organ is also at room temperature suspended until complete drying and complete the preparation of the drug by coloring the anatomical structures with oil paints diluted with polyacrylate varnish.

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