RU2501293C2 - Method of dewatering mycelium mass pleurotus oustreatus, grown in liquid medium - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to biotechnology and can be used for production of the planting mycelium mass Pleurotus oustreatus. The method comprises separating of the mycelium mass Pleurotus oustreatus, grown in liquid medium, from the liquid medium by passing it through the sterile gauze filter. Mycelium remaining on the filter is subjected to microwave exposure of 600 W and wavelength of 12 cm for 10-30 minutes in a vacuum, which depth is 0.003-0.005 MPa at a temperature of 25-30°C with saving of viability of the mycelium mass Pleurotus oustreatus.

EFFECT: it enables to save viability of the mycelium mass Pleurotus oustreatus.

Description

The method relates to agricultural biotechnology and industrial biotechnology.

Liquid mycelial mass is a good nutrient medium for the development of bacteria and lower fungi, such as Trichoderma spp., Penicillium spp., Aspergillus spp., Alternaria spp., Cladosporium spp., Arthrobotrys spp., Sclerotium spp., Mycor spp., Neurospora sitophila, Trichurus spirales, Chromelosporium fulvum. Pseudomonas spp. The processes of microbiological damage to the mycelial mass of Pleurotus oustreatus grown in a liquid medium and contaminated with mold spores can occur even at low storage temperatures. As a result of a decrease in water unbound to the cells and an increase in cell concentration, the mycelial mass of Pleurotus ostreatus increases immunity to yeast, molds and bacteria, which is an advantage over non-dehydrated liquid mycelium.

A known method of drying mycelial wastes of the antibiotic industry (Pat. RF №93036539). The method consists in the fact that the pasty mass of mycelial waste is sequentially directed to three conveyors moving in the opposite direction relative to the other in a closed drying chamber, at the same time air is heated to 70 ° C-90 ° C, followed by suction it through 3-5 channels, and air suction is carried out so that the temperature in the chamber varies sequentially along the vertical zones of air suction in the intervals of 40-50 ° C, 70-80 ° C, 40-50 ° C. The method allows to obtain a dry residue with a moisture content of 4% -6%. The dry mycelium obtained in this way can be deposited or sent to fur farms for feeding fur animals. The advantages of this method is the drying of large volumes of mycelial mass. The main disadvantage of this method is the death of the mycelial mass. A modified method is known, which may be a prototype (Pat. RF №2016522).

In the known method for producing dry feed mycelium, comprising heating and mixing a layer of raw mycelium, these operations are carried out under a vacuum of 0.05-0.06 MPa, during the processing, the humidity of the mycelium is continuously monitored by the value of the active power of the mixing motor of the device; in the period of falling drying speed, the mycelium temperature is raised to 90-100 ° C and maintained at this level until the end of the process, the process is stopped when the specified humidity value is reached. In this technical solution, heating and mixing the raw mycelium under vacuum at a given temperature regime provides a product with the required humidity. The disadvantage of this method is not sufficiently deep vacuum and high temperature, which leads to the death of the mycelium.

The purpose of the proposed method is the drying of Pleurotus oustreatus mycelium grown in a liquid medium under microwave exposure in vacuum while maintaining the viability of the mycelial mass.

When implementing this method, the drying temperature is reduced and the viability of the mycelium is preserved, and humidity is reduced.

The proposed method of dehydration of the mycelial mass of Pleurotus oustreatus grown in a liquid medium is as follows.

1. To separate the mycelial mass from the liquid medium, it is dehydrated by passing through a sterile gauze filter.

2. The mycelium remaining on the filter is a wet mass, which is subjected to microwave exposure in a vacuum. The optimum temperature of dehydration of the mycelial mass affects both the rate of moisture removal and the safety of a greater number of mycelial cells and should be in the range of 25-30 ° C. The wavelength of microwave electromagnetic radiation should be 12 cm. The microwave power should be 600 watts.

3. At a temperature of 25-30 ° C with the vacuum on, the depth of which should be 0.003-0.005 MPa, give an exposure of 10-30 minutes.

The proposed method allows dehydrated liquid mycelial mass to a moisture content of 25-35%.

The proposed method of dehydration of liquid mycelial mass differs from the known ones in that providing a lower drying temperature and a deeper vacuum during microwave exposure, allows you to maintain the viability of Pleurotus oustreatus mycelium.

Claims (1)

The method of dehydration of Pleurotus oustreatus mycelium grown in a liquid medium, characterized in that the drying process is carried out under microwave exposure with a power of 600 W and a wavelength of 12 cm for 10-30 minutes in vacuum, the depth of which is 0.003-0.005 MPa at a temperature of 25-30 ° C while maintaining the viability of the mycelial mass.