RU2485793C2 - "verva" biologically active fodder additive - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to farm industry, in particular, to a liquid biologically active fodder additive for animals and birds. The fodder additive contains an active substance in the form of water-soluble salts of triterpenic acids and is produced by way of extraction with a water solution of sodium hydroxide of milled green fir wood and the liquid phase filtering out from the solid one upon completion of the extraction process.

EFFECT: fodder additive feeding has stimulatory effect onto the whole organism as well as allows to increase animals and birds productiveness and enhance their resistance to various diseases.

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Description

The invention relates to agriculture, in particular to the use of a liquid feed additive from fir green wood, namely in the field of animal husbandry and poultry farming. The additive can be used to increase the productivity of animals and birds, increase resistance to various diseases, to provide a stimulating effect on the whole organism.

It is known the use of the drug "Abisib" in the form of an aqueous solution of extractives isolated from green wood fir [patent 2061491, cl. A61K 35/78, 1996]. The drug can be used in animal husbandry as a biostimulant and for the rehabilitation of the body.

It is known the use of biologically active feed additives "Pihtex" [patent 2216198, class. AK61K 35/78, 2001] obtained from coniferous raw materials. The invention is intended for use in the field of livestock, poultry, fishing.

The objective of the present invention is to obtain a product - a new biologically active liquid feed additive for animals and birds in order to increase the productivity of animals and birds, increase resistance to various diseases, to provide a stimulating effect on the whole body. This is the technical result.

The technical result is achieved by the fact that a liquid biologically active feed additive for animals and birds contains the active substance in the form of water-soluble salts of triterpene acids and is obtained by extracting chopped wood green fir from an aqueous solution of sodium hydroxide and filtering the liquid phase from solid at the end of the extraction process.

A method of obtaining a biologically active liquid feed additive is as follows. The chopped wood green fir is extracted with an aqueous solution of sodium hydroxide. At the end of the extraction process, the liquid phase is filtered off from the solid phase. The liquid phase is used as a dietary supplement for animals.

Before use, the feed additive must be mixed and diluted in water in a ratio of 1:10. The additive is introduced to animals and birds as a part of premixes, compound feeds and grain mixtures, juicy and roughage, as well as by evaporation. Recommended administration rates: for a bird, 100-500 ml per 1 ton of compound feed (1-5 g of active ingredient per 1 ton) per day; animals 1-5 ml / head per day.

The experimental use of a feed supplement from fir yielded positive results.

Example 1

The studies were performed in the SEC “Kortkeros-1” of the Komi Republic. The total duration of the experiment was 65 days. The feed additive from fir was used in the winter-spring period on the experimental group of dairy cows with an average milk yield of 4,500-5,000 kg of milk per lactation and fat content of 3.6-3.8%. In total, 18 cows that were 2–3 months of lactation were selected from the dairy herd. During lactation, all the cows involved in the experiment naturally decreased the milk yield from 20.6 liters at the beginning to 17.7 liters at the end. The amount of fat and protein in milk first increased slightly, and then were at the same level: 3.7% fat and 3.0% protein.

The feed additive in appropriate quantities was mixed with 200 g of sunflower meal and in dry form was fed to each animal individually from a bucket, daily, once a day, after morning milking during the entire period of the experiment. The additive was added to the sunflower meal by spraying from a household sprayer. For this, additive and water were poured into the tank of the sprayer in a ratio of 1: 1. The resulting working solution was thoroughly mixed.

Household diets, taking into account the productivity of cows, consisted of 5 kg of cereal hay, 25 kg of grass-mixed grass silage, 5-7 kg of feed granulated for dairy cows and heifers K-60-10 (280 g / l), 0.6 kg of loose sunflower meal , 0.8 kg of granulated beet pulp, 50 g of chalk and 100 g of common salt. 1 kg of dry matter of rations contained 9.7-9.8 MJ of metabolic energy, 11.5-12.1% of crude protein, 7.5-8.2 of digestible protein, 26-27% of crude fiber, 7.1 g calcium, 4.3-4.6 phosphorus, 2.7-2.8 g of magnesium.

Taking into account the given feeds and their residues showed that the introduction of feed additives from fir into the diet of cows did not affect the total feed intake of the main diet. From the very first day, cows willingly and completely ate dry sunflower meal enriched with a feed additive.

Studies were carried out in three stages: the first 25 days were administered 0.5 and 1 ml of the supplement per day per head, then 25 days - 1 and 2 ml and the last 15 days - 2 and 4 ml.

As a result of the experiments, the influence of a liquid feed additive on the yield of cows was revealed (Table 1-3).

The analysis showed that the use of feed additives does not affect the milk fat and milk protein of cows. The percentage of fat and protein in the milk of all cows ranged from 3.6-3.7%.

In the process of research, a positive effect of the feed additive on the reproductive qualities of cows was revealed, in particular on the duration of the service period, the duration of which was reduced by 3-10 days.

Example 2

The studies were performed in the SEC “Palevitsy” of the Komi Republic.

The feed supplement from fir (in doses from 1 to 5 ml per 1 head per day) was used in the winter-spring period on dairy cows of Ayrshire breed. The average milk yield of cows was 4500-5000 kg of milk per lactation and with a fat content of 4.0-4.2%. In addition to the main diet, the cows participating in the experiment received an additional amount of feed additive mixed with 100 g of wheat bran.

In the process of research, a positive tendency was found to affect the dose of feed additives equal to 3 ml on the yield of cows. Feeding 3 ml of the additive increased the milk yield from 23.8 liters in the control to 29 liters per day, or 5.2 liters. At the same time, the use of the additive did not adversely affect the basic biochemical parameters of blood and the milk fat content of cows.

A tendency was found to decrease the duration of the service period for cows fed the feed supplement. A dose of 4 ml was especially effective. So, feeding 4 ml of the supplement reduced the service period from 105 days in the control to 72, or by 33 days. Other dosages of the feed additive reduced the duration of the service period compared with the control by 9-17 days.

Example 3

The antiviral properties of the feed additive (effects on avian influenza viruses (GP) and Newcastle disease (NB)) were studied on 380 chicken embryos of a 10-day incubation period.

The feed additive was diluted in water in a ratio of 1: 5, 1:50, 1: 500, then the solutions were combined in equal volumes with the NB virus and the GP virus, thus obtaining the final dilutions of the additive: 1:10, 1: 100, 1: 1000. The NB virus diluted with sterile physiological saline 1: 1 and the GP virus diluted with sterile saline 1: 1 were used as controls.

The studies revealed a pronounced virucidal effect of the feed additive in relation to the Newcastle disease and avian influenza viruses (Table 4).

Example 4

To determine the effectiveness of the use of feed additives in poultry rearing, an experiment was conducted on broiler chickens from daily to 35 days of age. Previously, the chemical composition of the additive was investigated (Table 5). In the main diet, the supplement was introduced at a dose of 50 ml / t of feed.

It was found that the use of the additive has a growth-promoting effect when growing broilers and allows to increase the productivity of poultry (Table 6). Data on the vitamin content in the liver of chickens (table 7) indicate the positive effect of feed additives.

Table 1 Average milk yield in the first period of the experiment, depending on the dose of the supplement Dose of supplements per 1 head, ml / day Average milk yield, l 0 18.70 0.5 20,42 one 22.63

table 2 Average milk yield in the second period of the experiment, depending on the dose of the supplement Dose of supplements per 1 head, ml / day Average milk yield, l 0 17.71 one 19.31 2 21.14

Table 3 Average milk yield in the third period of the experiment, depending on the dose of the supplement Dose of supplements per 1 head, ml / day Average milk yield, l 0 16,48 2 17.40 four 19.18

Table 4 The virucidal effect of the feed additive on the viruses NB and GP No. p / p Breeding supplements NB virus titer GP virus titer one 1:10 10 ^4,5 10 ^2.7 2 1: 100 10 ^8.7 10 ^4.9 3 1: 1000 10 ^10.1 10 ^6.9 four 10 ^10.5 10 ^7.1

Table 5 Quality indicators of feed supplement for fir Indicators unit of measurement amount Crude protein % 0.38 Raw ash % 4.96 Calcium % 1.05 Phosphorus % 0.013 Manganese mg / kg 48 Iron mg / kg 21 Copper mg / kg 2.2 Zinc mg / g eleven Carotenoids mcg / g 4.65

Table 6 Broiler Growth Efficiency Indicators Group The control Experience Weight of chickens, g, aged: per diem 42 42 2 weeks 401 419 3 weeks 801 838 35 days 2008 2047 The safety of livestock,% 97.2 97.2 The average daily gain in live weight, g 57.4 58.5

Table 7 The vitamin content in the liver of broiler chickens,% Vitamin amount The control Experience A, mcg / g 50.1 73.6 B ₂ μg / g 10.3 10.9

Claims (1)

A liquid biologically active feed additive for animals and birds, characterized in that it contains the active substance in the form of water-soluble salts of triterpene acids and is obtained by extracting chopped wood green fir from an aqueous solution of sodium hydroxide and filtering the liquid phase from solid at the end of the extraction process.