RU2485779C1 - Fungicidal composition for seed treatment - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to plant protection agents. Disclosed is a preparation having fungicidal properties, where the active components used are terbuconazole, imazalil and prochloraz or metalaxyl. The ratio terbuconazole:imazalil:prochloraz/metalaxyl is equal to 1:(0.5-15.0):(0.5-15.0), with consumption rate of active substances of 45-300 g/t seeds.

EFFECT: invention widens the range of biological action against snow mould, increases biological efficiency against root rot.

4 tbl, 2 ex

Description

The invention relates to plant protection products, in particular to a preparation having fungicidal properties, where tebuconazole, imazalil and prochlorase or metalaxyl are used as active components.

Known fungicidal protectants for treating seeds of agricultural crops against various diseases based on tebuconazole - [(RS) -4,4-dimethyl-3- (1H-1,2,4-tyrazol-1-ylmethyl) -1-n-chlorophenylpentan- 3-ol] in the form of wetting powders - Raxil and Agrosil, in the form of a suspension concentrate - Raxil / Handbook of pesticides and agrochemicals approved for use on the territory of the Russian Federation, 2011 /.

Known fungicidal preparation containing Imazalil - 1- (β-allyloxy-2,4-dichlorophenethyl) imidazole used against powdery mildew and other cereal diseases / Handbook. Pesticides and plant growth regulators, NN Melnikov, M., 1995 /.

Known fungicide containing Prochloraz - 1- / N-propyl-N- / 2- (2,4,6-trichlorophenoxy) ethyl / carbamoyl / imidazole - a wide spectrum of action for combating diseases of grain and other crops / Reference. Pesticides and plant growth regulators, NN Melnikov, M., 2001 /.

Known fungicide containing Metalaxyl - N- (2.6-xylyl) -N- (2-methoxyacetyl) -DL-alanine methyl ether - produced in the form of a wetting powder. Metalaxil is used to combat diseases of potatoes, tomatoes, cucumbers, cabbage, beets / Reference. Pesticides and plant growth regulators, NN Melnikov, M., 1995 /.

Based on the patent search, the following patents were selected relating to the fungicides listed above.

There are various formulations based on tebuconazole containing auxiliary substances (US Pat. RF 2138525, 2238649, 2232504). The disadvantage of these compositions is the low biological activity, a narrow spectrum of action.

To expand the spectrum of action and enhance the biological activity of tebuconazole used in various mixed preparations (US Pat. RF No. 2024228, 2192743, 2040899). So, for example, in RF patent No. 2040899 an emulsion concentrate based on two active compounds of tebuconazole and 4- (2,2-difluoro-1,3-benzodioxol-7-yl) -1H-pyrrole-3-carbonitrile containing auxiliary substances is described , in the patent of the Russian Federation No. 2024228 a fungicidal composition is described in the form of an emulsion concentrate based on a mixture of tebuconazole with propiconazole.

To expand the spectrum of action and enhance the biological activity of imazalil, various auxiliary substances are also used, for example, in US Pat. RF №2382554 describes the composition of the drug containing imazalil with alkoxylated amines.

To expand the spectrum of action and enhance the biological activity of metalaxyl, a fungicidal composition based on metalaxyl is used, which additionally contains other active substances, i.e. dimetomorph and famoxadone (US Pat. RF 2181004). The composition has increased biological activity and reduced consumption rates.

The effectiveness and technological characteristics of these drugs are insufficient.

The most preferred for use in agriculture are liquid preparations, since they do not dust during the preparation of the working solution, which reduces their toxicity and increases safety in their use.

The closest technical solution to the claimed composition is a fungicidal composition comprising tebuconazole and imazalil as active substances, as well as excipients (US Pat. RF 2352114). The ratio of tebuconazole: imazalil is 0.35-1.55: 1. The drug is used to protect crops at a rate of consumption of 0.3-0.4 l / t against smut diseases, root rot, powdery mildew.

The fungicidal compositions listed above have the main drawback: a wide range of fungicidal effects, for example, preparations are not used against snow mold of winter crops.

The viability of mycelium and conidia of snow mold is maintained even at a temperature of -33 ° C. Under natural conditions, such a temperature occurs in winter only in the absence of snow cover. Snowy winters, low temperatures, high moisture supply, extended snow melting, and increased acidity of soils determine the danger of frequent emergence of infectious aging. In the Northwestern and Central regions in the epiphytotic years (3 times out of 10 years) the development of the disease is 40-50%, death - 15-20%. On separate fields, crops can completely die (Sanin et al., 1999; Zakharenko et al., 2000). Protective measures and crop rotation do not give satisfactory results.

The purpose of this invention is the expansion of the spectrum of biological action against snow mold for winter crops, increasing the biological effectiveness against various root rot.

This goal is achieved through the use of a fungicidal composition for seed treatment, including tebuconazole and imazalil as active substances. Moreover, the composition additionally contains, as another active component, prochlorase or metalaxyl with the ratio tebuconazole: imazalil: prochloraz / metalaxyl 1: (0.5-15.0) :( 0.5-15.0) at a rate of consumption of the sum of active substances 45 -300 g / t seeds.

Fungicides are often combined in a mixture for three main reasons:

- to expand the spectrum of fungicidal activity in order to control several diseases;

- to achieve a synergistic effect of the action of fungicides, in which the total activity increases, and the concentration of active substances can be reduced without loss of activity;

- in order to delay the pathogen resistance to one of the components of the mixture.

Example 1

An experimental study of the fungicidal and fungistatic activity of the claimed preparations containing prochlorase as an additional active substance was carried out on a Fusarium nivale isolate (snow mold).

In the experiment, the following composition was investigated: tebuconazole: imazalil: cool in a ratio of 1: 1: 1.

The drug was introduced into a potato-agar medium. The composition of the medium: potato broth (200 g / l), sucrose (15 g / l), agar (25 g / l).

Inoculation of the isolate was carried out by die cuts of 0.5 × 0.5 mm in Petri dishes on a layer of medium. Growing conditions: in a thermostat at a temperature of 22 ° C.

Accounting for growth was carried out on the 4th and 10th day from the moment of sowing by measuring the diameter of the mycelium.

The growth rate (CP) of the fungi was calculated as the ratio of the diameter of the mycelium growth in the experimental version to the control, expressed in%. Efficiency (E) was calculated as E = 100% -CP.

Four repetition.

The expected efficacies E _Waiting. calculated by the Abbott-Colby formula

$$E_{\mathrm{about}\mathrm{well}\mathrm{and}d.}=A+B-\frac{A\times B}{\mathrm{one\; hundred}},$$

where A and B are the efficacy for separately applied fungicides.

If the ratio between the observed experimental efficacy (E _exp.) And the expected efficiency (E _ozhid.) - synergy factor (SF), - more than 1, the mixture displays a synergistic effect:

$$\mathrm{FROM}F=\frac{E_{\mathrm{uh}\mathrm{to}\mathrm{from}P.}}{E_{\mathrm{about}\mathrm{well}\mathrm{and}d.}}$$

The research results are shown in table 1.

Thus, it can be seen from the table that the introduction of the third component of prochlorase into the mixture exhibits a synergistic effect against Fusarium nivale.

Example 2

Similarly, experimental studies of the fungicidal and fungistatic activity of the claimed preparations containing metalaxyl as an additional active substance were carried out. The experiments were conducted on isolate Bipolaris sorokiniana. The following composition was investigated in the experiment: tebuconazole: imazalil: metalaxyl at a ratio of 1: 1: 1.

The research results are shown in table 2.

Thus, it can be seen from the table that the introduction of a third component (metalaxyl) into the mixture leads to a synergistic effect of the three-component mixture against Bipolaris sorokiniana (helminthosporious root rot).

The proposed preparations have high biological activity against snow mold on winter cereals, prochlorase and metalaxyl have shown synergistic properties with respect to the mixture (tebuconazole + imazalil).

The high biological activity of the combinations of biologically active substances according to this invention is visible from the examples below. While individual active substances exhibit insufficient fungicidal activity, the claimed combinations exhibit activity that exceeds a simple summation of activities.

To obtain a suitable ready-to-use preparation, biologically active substances in the claimed proportions are mixed with excipients and the preparation is diluted with water to the required concentration. Ready solution pickle seeds.

The results of biological experiments are summarized in tables 3 and 4.

Table 1 Fungicidal activity of drugs at various concentrations in relation to Fusarium nivale Active components Dose, ml / l _Exp. Uh, _wait. SF Tebuconazole + imazalil 0.0002 + 0.0002 12.0 Cool 0,0002 8.2 Tebuconazole + imazalil + chloraz 0.0002 + 0.0002 + 0.0002 52.9 24.6 2.15 Tebuconazole + imazalil 0.002 + 0.002 32,5 Cool 0.002 19.5 Tebuconazole + imazalil + chloraz 0.002 + 0.002 + 0.002 97.6 57.4 1.70

Table 2. Fungicidal activity of drugs at various concentrations in relation to Bipolaris sorokiniana Active components Dose, ml / l _Exp. Uh, _wait. SF Tebuconazole + imazalil 0.0002 + 0.0002 11,4 Metalaxyl 0,0002 9.5 - - Tebuconazole + imazalil + metalaxyl 0.0002 + 0.0002 + 0.0002 58.4 19.8 2.95 Tebuconazole + imazalil 0.002 + 0.002 25.0 Metalaxyl 0.002 26.3 Tebuconazole + imazalil + metalaxyl 0.002 + 0.002 + 0.002 82.7 44.7 1.85

Table 3 The effectiveness of the claimed fungicidal composition (winter wheat) in the conditions of small-plot experiments. Moist seed dressing, 4x repetition. №№ The ratio of DV - Tebuconazole: Imazalil: Prochloraz / Metalaxyl Consumption rate ∑ DV g / t of seeds Biposo Development,% Efficiency% one Tebuconazole: Imazalil: Prochloraz = 1: 0.5: 0.5 45 6.0 66.7 2 Tebuconazole: Imazalil: Prochloraz = 1: 1: 4 300 2,8 84,4 3 Tebuconazole: Imazalil: Prochloraz = 1: 1: 15 102 4.0 77.8 four Tebuconazole: Imazalil: Prochloraz = 1: 15: 5 63 4,5 75.0 5 Tebuconazole: Imazalil: Metalaxyl = 1: 0.5: 0.5 60 5,0 72,2 6 Tebuconazole: Imazalil: Metalaxyl = 1: 1: 15 119 3,1 82.8 7 Tebuconazole: Imazalil: Metalaxyl = 1: 1.6: 1.3 72 4.9 72.8 8 Tebuconazole: Imazalil: Metalaxyl = 1: 15: 5 105 3.3 81.7 9 Tebuconazole: Imazalil: = 1: 1.6, reference 64 6.2 65.6 10 - the control - 18.0 - BIPOSO - Bipolaris sorokiniana (helminthosporious root rot)

Table 4 The effectiveness of the claimed fungicidal composition (winter wheat) in the conditions of small-plot experiments. Moist seed dressing, 4x repetition. №№ The ratio of DV - Tebuconazole: Imazalil: Prochloraz / Metalaxyl Consumption rate ∑ DV g / t of seeds Fusarium nivale Development,% Efficiency% one Tebuconazole: Imazalil: Prochloraz = 1: 0.5: 0.5 45 12.3 48.5 2 Tebuconazole: Imazalil: Prochloraz = 1: 1: 4 300 3.8 84.1 3 Tebuconazole: Imazalil: Prochloraz = 1: 1: 15 102 6.7 72.0 four Tebuconazole: Imazalil: Prochloraz = 1: 15: 5 63 10.8 54.8 5 Tebuconazole: Imazalil: Metalaxyl = 1: 0.5: 0.5 45 15.7 34.3 6 Tebuconazole: Imazalil: Metalaxyl = 1: 1: 15 119 12.1 49.4 7 Tebuconazole: Imazalil: Metalaxyl = 1: 1.6: 1.3 72 10.5 56.1 8 Tebuconazole: Imazalil: Metalaxyl = 1: 15: 5 105 8.3 65.3 9 Tebuconazole: Imazalil: = 1: 1.6 64 18.8 21.3 10 - the control - 23.9 -

Claims (1)

A fungicidal composition for seed dressing, comprising tebuconazole and imazalil as active substances, characterized in that the composition further comprises prochlorase or metalaxyl as another active component, the ratio of tebuconazole: imazalil: prochlorase / metalaxyl 1: (0.5-15.0 ) :( 0.5-15.0) at a rate of consumption of the amount of active substances of 45-300 g / t of seeds.