RU2469738C1 - Method of carrying out of superovulation in cows-donors of embryos - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method includes injection of the preparation "FSH-super" and the preparation of prostaglandin F2α. On the day of artificial insemination the cows-donors are additionally injected with homeopathic preparation "Ovariovit" at the dose of 4.9-5.1 ml per head in a single dose.

EFFECT: method enables to obtain a greater number of embryos and to improve their quality.

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Description

The invention relates to biotechnology and improves the efficiency of superovulation in cattle.

An increase in the efficiency of embryo transplantation is possible only if ovulation occurs not in one but in several germ cells (superovulation phenomenon) and in the future they will fully fertilize by seed.

Known methods for conducting superovulation in cows using gonadotropins.

A widely used gonadotropin for conducting superovulation is FFA. To ensure the growth of several follicles and their subsequent ovulation, FFAs are injected 4-5 days before the expected hunt. This is achieved by the fact that the hormonal treatment of the donor begins 2-3 days before the natural or induced regression of the corpus luteum. In the first case, the drug is administered in the transition period from the luteal to the follicular phase of the reproductive cycle on days 16-17 and then, when the first signs appear and during the expected hunt, 2000 IE CG are injected intravenously (3).

The advantage of this method is the low cost of FFA, the ability to get the drug directly at the enterprise producing embryos. The disadvantage of this method is the high anaphylactogenicity of FFA.

A known method of conducting superovulation according to the following scheme (Irish method):

Cycle days 16-17th 1500-3000 units of HFFA intramuscularly 19-20th 10 mg of estradiol benzoate intramuscularly 21st Sexual hunting (0 day). 1500-3000 IU of chorionic gonadotropin intravenously 1st First insemination 2nd Second insemination 5th Surgical embryo extraction 6-8th Non-surgical embryo extraction

The advantage of this method is a single use of gonadotropin. However, this method requires the additional use of estradiol benzoate and chorionic gonadotropin. In addition, this method is extended in time, which causes a rise in the cost of obtaining embryos in connection with feeding and donor maintenance (1).

A known method of conducting superovulation according to the following scheme (Danish method):

Cycle days 8-12th 1500-3000 units of HFFA intramuscularly 10-14th 25 mg PG- _2α or 500 mcg cloprostenol intramuscularly After 48 hours 0 Sexual hunt 1st First insemination 2nd Second insemination 6-8th Non-surgical embryo extraction

The advantage of this method is a short period of hormonal treatment of the animal - the donor of the embryo. However, the prolonged action of gonadotropins does not allow to obtain a stable result in the form of superovulation and the quality of embryos (1).

A known method of conducting superovulation using the drug "FSH-p":

Cycle days 8-10th FSH-p 15 mg 9-11th FSH-p 12 mg 10-12th FSH-p 10 mg Prostaglandin 500 mcg - 30 mg 11-13th FSH-p 5 mg 12-14th Hunting and insemination 19-21th Non-surgical embryo extraction

The advantage of this method is the uniform effect of the drug on the ovary and obtaining a large number of embryos suitable for freezing and storage. The disadvantage is the high cost of the drug (4).

The closest technical solution is the method of conducting superovulation using the drug "FSH-super."

To stimulate polyovulation (superovulation) in embryo donor cows, starting from day 9-10 of the reproductive cycle, they are given the drug “FSH-super” in a dose of 6 units. Armor's Standard (AE) (120 I.E.) twice a day every 12 hours. On the third day after the start of administration, one of the preparations of F _2α (estrofan, clathraprostin, etc.) is administered at the same time in the morning and evening at the same time as FSH-super. Identification of signs of hunting in cows begins after 36-48 hours from the time of administration of prostaglandin F _2α . As animals enter the hunt, they are artificially inseminated 2-3 times with an interval of 12 hours. Embryos from donor cows are removed at the time necessary to obtain embryos of the desired stages (on days 7-8), considering the day of the first insemination of animals as day zero (2).

The advantage of this method is the low cost of the drugs used and the high yield of embryos.

The disadvantage of this method is the lack of nonspecific stimulation of the ovaries and uterus.

The aim of the invention is to increase the effectiveness of superovulation in donor cows of embryos and improve their quality using homeopathic preparations that have a nonspecific effect on the ovaries and uterus.

This goal is achieved by the fact that when carrying out superovulation using the FSH-super preparation and prostaglandin on the day of artificial insemination, donor cows receive an intramuscular injection of the homeopathic preparation Ovariovit at a dose of 4.9-5.1 ml per head.

Ovariovit - solution for injection TU 9337-007-73027337-05. Composition: Calcium carbonicum D6, Damiana D4, Aquilegia D4, Pulsatilla D8, Aurum jodatum D6, Sepia D10, Aristolochia D4.

Damiana D4, Aquilegia D4, Aristolochia D4, Sepia D10, Pulsatilla D4 - are considered the main ones in homeopathy for hormonal dysfunction, infertility, and violation of the sexual cycle (acyclia, infantile genital organs), including those caused by insufficiency of the anterior pituitary gland.

Aurum jodatum D6 - recommended for chronic endometritis, uterine subinvolution, decreased tone, uterine prolapse, cystic ovarian degeneration.

Calcium carbonicum D6 - the main remedy for impaired calcium metabolism. It is also used for chronic diseases of the mucous membranes and proliferative processes on them.

A comparative analysis of the claimed and used methods of conducting superovulation in donor cows of embryos allows us to identify signs that distinguish the proposed technical solution from the prototype: increasing the effectiveness of the influence of follicle-stimulating hormone in superovulation, as well as improving the quality of the resulting embryos, is achieved by intramuscular injection of the homeopathic medicine “Ovariovit”, which contains the corresponding components are Calcium carbonicum D6, Damiana D4, Aquilegia D4, Pulsatilla D8, Aurum jodatum D6, Sepia D10, Aristolochia D4. The injection of the drug “Ovariovit” is carried out on the day of artificial insemination at a dose of 4.9-5.1 ml per head once.

From the literature available to us, the use of Ovariovit is unknown to increase the efficiency of superovulation in donor cows of embryos together with follicle-stimulating hormone.

Thus, the claimed method for conducting superovulation in donor cows of embryos meets the criterion of "novelty." The inventive method has an inventive step, as for the average specialist it clearly does not follow from the prior art: neither in the instructions nor in the recommendations for embryo transplantation, the use of homeopathic medicines, in particular “Ovariovit”, is not described. The method is industrially applicable, as it can be used to stimulate superovulation in donor cows.

The authors propose to use “FSH-super”, prostaglandin and optionally “Ovariovit” drug, which is injected to animals on the day of artificial insemination in a dose of 4.9-5.1 ml per head, to increase the efficiency of superovulation in donor cows and improve the quality of embryos. .

Execution example

To implement the proposed method for conducting superovulation in cows-donor embryos, scientific and production experience was set.

In the conditions of OAO PZ "Russia" in the Sosnovsky district of the Chelyabinsk region, two groups of cows with 5 animals each were formed on the principle of analogs: control and experimental.

Superovulation in animals was carried out according to the scheme shown in the table:

Cycle days Control group Experienced group 10th “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰ “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰ 11th “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰ “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰ 12th “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰
Prostaglandin 500 mcg at 8 ⁰⁰ and 250 mcg at 20 ⁰⁰ “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰
Prostaglandin 500 mcg at 8 ⁰⁰ and 250 mcg at 20 ⁰⁰ 13th “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰ “FSG-super” 6 AE at 8 ⁰⁰ and at 20 ⁰⁰ 14th Hunting and insemination Hunting and insemination, injection "Ovariovit" 5 ml intramuscularly 21st Non-surgical embryo extraction Non-surgical embryo extraction

Results of non-surgical embryo extraction

Indicator Control group Experienced group The number of embryos per donor 3.6 4.8 The number of embryos per donor suitable for transplantation 3.0 4.8 The number of embryos per donor unsuitable for transplantation 0.6 0

The table shows that the cows of the experimental group received 1.2 embryos more than in the control group. Moreover, all embryos from cows to the experimental group were suitable for transplantation, while 0.6 embryos received from animals in the control group were not suitable for transplantation. Data are based on one animal.

Thus, the proposed method allows to obtain a larger number of embryos from donor cows and improve their quality.

Literature

1. Valyushkin K.D., Medvedev G.F. Obstetrics, gynecology and biotechnology of animal reproduction: Textbook, 2nd ed., Revised. and add. - Мn .: Urajay, 2001. - P.320.

2. Instruction for the use of “FSH-super” to stimulate ovarian function in female animals and polyovulation in embryo donor cows. Approved by Deputy Head of the Veterinary Department B.A. Kobzev on April 23, 1996. Registration number of the drug is PVR-2-1.0 / 00269.

3. Prokofiev M.I. Regulation of breeding of farm animals. - L .: Nauka, 1983 .-- P.182.

4. Ernst L.K., Sergeev N.I. Transplantation of embryos of farm animals. - M.: Agropromizdat, 1989 .-- P.117.

Claims (1)

A method of conducting superovulation in embryo donor cows, including an injection of FSH-super and prostaglandin F2α, characterized in that the animals are injected with a homeopathic preparation Ovariovit at a dose of 4.9-5.1 ml when superovulating on the day of artificial insemination on the head once.