RU2446713C1 - "pentamarin" biologically active food additive and its production method - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to biologically active food additives intended for prevention of metabolic derangements, cardiovascular and other disorders and adjuvant therapy for reduction of their development risk. The biologically active additive includes a fat modulus represented by marine animal oil with polyunsaturated omega-3 acid content no less than 20 wt % and a carbohydrate modulus that are placed into individual chambers at a ratio of (1-3.33):(23.33-25.67) respectively. Mixing of the fat and carbohydrate moduli is performed immediately prior to consumption. The carbohydrate modulus includes, wt %: fructose - (10-12), sodium alginate - (0.7-1.5), water - balance. The biologically active additive production method envisages preparation of the carbohydrate modulus: sodium alginate and fructose are dissolved in individual vessels with boiled water with temperature no higher than 100°C, added into boiling water while stirring. After the sodium alginate and fructose complete dissolution the solutions are mixed till the required components ratio is reached, cooled till the temperature is 50-65°C and filtered immediately prior to the carbohydrate modulus placement into the chamber.

EFFECT: invention allows to produce a biologically active additive with extended storage life - no less than 24 months, without detriment to quality characteristics and with preservation of good organoleptic indices.

4 cl, 3 ex

Description

The invention relates to biologically active food additives intended for prophylaxis, adjuvant therapy in order to reduce the risk of developing metabolic disorders, cardiovascular and other diseases.

Known pharmaceutical agent, which includes animal fat, bee honey, mercuric chloride and alcohol (RF patent No. 2071778, A61K 33/28, publ. 1997). The essence of the method consists in preliminary separate dissolution of mercuric chloride and bee honey in 96 ° ethanol. Both alcohol solutions obtained are mixed for 15-20 minutes and the resulting mixture is kept in a dark place at a temperature of 18-22 ° C for 2-3 days. Then, in a softened base, which is animal fat or a mixture of fats, an alcoholic solution containing mercuric chloride and bee honey is introduced with stirring with repeated weight keeping in a dark place at 18-22 ° C for 3-6 days.

This drug has antitumor activity and is not intended for the prevention of cardiovascular diseases. It cannot be recommended for widespread use, since it contains mercury salt - mercury chloride II. The disadvantages should also include a short shelf life and the duration of its receipt. The preparation process takes from 5 to 9 days.

Known food biologically active additives (BAA) from seafood, which can be used in diet therapy for the prevention or treatment of cardiovascular diseases. Supplements contain edible fish oil and biologically active substances (options): alcoholic extract of phospholipid concentrate, alcoholic extract of phospholipid concentrate with saponins obtained from marine aquatic organisms such as cucumaria, sea urchins and stars, herring, etc. (RF patent No. 2225210, A61K 35/00, A61K 31/00, A23L 1/325, publ. 03/10/2004).

The disadvantages of the present invention include the difficulty of obtaining one of the components of dietary supplements, namely the phospholipid concentrate. To obtain it requires not only a number of chemicals, equipment, but also the source of raw materials - seafood, which in themselves are rich sources of biologically active substances, vitamins and minerals. Cucumaria, sea urchin caviar, herring are used to prepare a significant number of culinary dishes. Moreover, the addition of a phospholipid concentrate to edible fish oil enhances the action of only the fat component of dietary supplements, since carbohydrates are not present in this supplement.

The closest technical solution for the purpose and the number of similar features to the claimed invention is a biologically active food supplement (BAA) "Marivit" and the method for its preparation. Supplements are obtained by mixing a fat-honey mixture, including bee honey and marine mammalian fat, with an aqueous solution of sodium alginate, in the following ratio of ingredients, wt.%: Bee honey - 15-20, marine mammalian fat 4-12, sodium alginate - 0.5 -4.5, water - the rest (RF patent No. 2253328, A23L 1/30, 1/325, A23D 7/00, publ. 06/10/2005).

To obtain the target product according to the invention, an aqueous solution of sodium alginate is preliminarily prepared, for this purpose sodium alginate is taken, placed in a reactor with a stirrer, distilled water heated to boiling is added, and left to swell with stirring for 24-72 hours at normal temperature, after whereby the resulting sodium alginate solution is subjected to vacuum filtration (using a Bunsen funnel and a Bunsen flask using three to four layers of calico).

Bee honey and marine mammalian fat are heated in separate open stainless steel containers to 100-120 ° C with stirring, subjected to vacuum filtration and mixed at the same temperature in a separate open container, after which the resulting fat-honey mixture is subjected to vacuum filtration.

The filtered aqueous alginate solution is heated to 100-120 ° C and mixed with the fat-and-honey mixture at the same temperature, after which the mixture is also subjected to vacuum filtration. Then the obtained biologically active additive is poured at a temperature of 100-120 ° C in vacuum into glass bottles with a capacity of up to 30 ml and hermetically sealed. Store at room temperature in a dark place for no more than two months without deterioration in the quality of dietary supplements.

The disadvantages of this method of producing dietary supplements are:

- the complexity and duration of the process of its preparation, requiring separate preparation of each component, and in separate containers, including heating to 100-120 ° C and vacuum filtration (filtered 4 times: sodium alginate, honey, marine mammalian fat and the finished product before packaging), as well as the duration of the preparation of an aqueous solution of sodium alginate, requiring its swelling within 24-72 hours;

- a significant cost of the target product due to the use of additional equipment and electric energy for heating;

- heating of sodium alginate at 100-120 ° C leads to a partial destruction of polymer bonds, which ultimately leads to not only a deterioration in the biological activity of dietary supplements, but also to a reduction in the shelf life of finished products;

- during prolonged sedimentation of sodium alginate (within 24-72 hours), yeast / mold forms for swelling, which ultimately leads to a deterioration in the biological activity of dietary supplements and to a reduction in its shelf life;

- the use of bee honey in the dietary supplement during storage of the target product leads to its foaming, souring, which also reduces the shelf life of the target product. Bee honey in its composition contains different composition and type of yeast. The presence of moisture starts the process of honey fermentation, in an aqueous solution of sodium alginate, honey is rapidly fermented within 2 months, a white foam with an aftertaste of alcohol forms;

- not in the best way the quality of dietary supplements is affected by the prolonged joint presence in it of polyunsaturated fatty acids, honey and sodium alginate, since they are substances of different nature. They have different physical and chemical properties, these substances are quite difficult to combine simultaneously in one drug, without reducing the quality of the individual components and without reducing the shelf life. As it turned out, fish oils are extremely sensitive to the presence of water. In the presence of water, the process of hydrolysis is accelerated, the total content of free fatty acids increases, and mechanisms of further degradation of fat are triggered, which leads to a deterioration in the quality of the target product and a decrease in its shelf life.

The problem solved by the invention is to improve the quality and shelf life of biologically active food additives; simplification, reduction and reduction of the cost of the process of its receipt, as well as the expansion of the range of dietary supplements.

The problem is solved in that in a known biologically active food supplement containing a fat module represented by marine mammalian fat with an omega-3 polyunsaturated acid content of at least 20 wt.%, And the carbohydrate module, according to the invention, the fat and carbohydrate modules are placed in separate chambers, with a ratio of (1-3.33) :( 23.33-25.67), respectively. As a carbohydrate module, a mixture of fructose, sodium alginate and water is used, in the following ratio of components, wt.%:

Fructose 10-12 Sodium Alginate 0.7-1.5 Water rest.

Mixing fat and carbohydrate modules is carried out immediately before the use of dietary supplements.

The use of fructose in the carbohydrate module allows to increase the shelf life of dietary supplements, simplifies the method of its production, and placing it separately from the fat component allows avoiding acidification of dietary supplements (formation of free fatty acids) and thereby contributes to the preservation of all biological properties of dietary supplements for a long time. Fructose as a monosaccharide belongs to carbohydrates and is an important chain in the metabolism. Of all types of sugar, fructose has the highest degree of sweetness. Once in the gastrointestinal tract, it is easily absorbed into the blood. Absorbed fructose is able to participate in the same metabolic processes as glucose, fully replacing it in situations in which glucose is not absorbed by the cells, which occurs in patients with diabetes mellitus. At the same time, firstly, the absorption of readily available fats and carbohydrates slows down, which is very important for patients with diabetes mellitus. Secondly, it binds and removes cholesterol, heavy metals and other toxic substances that form or enter the intestines from the body. Thirdly, fructose is one of the effective means for the restoration of intestinal microflora. The undeniable benefit of fructose is that it reduces the risk of caries and inflammatory diseases in the oral cavity by 20-30% compared with sugar. It is believed that it helps the liver process alcohol and turn the products of its incomplete decay into harmless substances. Regular use of dietary supplements containing fructose will help normalize carbohydrate metabolism, lower blood cholesterol, thereby reducing the risk of cardiovascular disease.

Achieving the claimed technical result is provided by the introduction of 10-12% fructose into the composition of the carbohydrate module of dietary supplement. When the fructose content is less than 10% in dietary supplements, the above effects are not enough manifested. And with an increase in the fructose content of more than 14%, undesirable effects begin to appear, in particular, irritable bowel syndrome can manifest itself. Irritable bowel syndrome usually means intestinal dysfunctions (constipation, diarrhea, flatulence, abdominal pain) without obvious organic changes in the gastrointestinal tract. This syndrome is eliminated by using sodium alginate in the dietary supplement.

The introduction of 0.7-1.5% sodium alginate into the carbohydrate module also helps to achieve the claimed technical result, primarily increasing the shelf life of dietary supplements by mixing with fructose, which is resistant to oxidation, as well as combining it with polyunsaturated fatty omega-3 acids directly during the use of dietary supplements by humans. Sodium alginate in the human stomach under the influence of hydrochloric acid contained therein turns into alginic acid, which is not absorbed in the intestine. It has the property of swelling and has a gentle enveloping effect on the walls of the gastrointestinal tract, while at the same time contributing to a significant weakening of pathological reflexes, including pain. The combination of alginic acid with sodium reduces excessive intestinal motility, which leads to normal stool. Sodium alginate, in addition to all of the above properties, has a hemostatic effect, which is due to a reduction in the polymerization time of the fibrin monomer, the final stage of blood coagulation, due to the presence of carboxyl groups in the polymer molecule of alginic acid. Alginic acid and its salts are effective enterosorbents that can bind and remove heavy metals and radionuclides from the body, accelerate wound healing, and lower blood cholesterol.

The combined presence of fructose and sodium alginate complement each other's positive properties and contribute to the achievement of the claimed technical result.

The task and the claimed technical result are also achieved by the method of obtaining the proposed dietary supplement. In a known method for producing a biologically active food supplement, comprising mixing a fat module represented by marine mammalian fat with an omega-3 polyunsaturated acid content of at least 20 wt.% And a carbohydrate module according to the invention, a mixture of fructose, alginate is taken as a carbohydrate module sodium and water, in the following ratio of components, wt.%:

Fructose 10-12 Sodium Alginate 0.7-1.5 Water rest,

while the fat and carbohydrate modules are placed in separate chambers, with a ratio of (1-3.33) :( 23.33-25.67).

The mixture of fat and carbohydrate modules is carried out immediately before use.

The preparation of the carbohydrate module is carried out as follows: sodium alginate and fructose are dissolved in separate containers with boiled water having a temperature of no higher than 100 ° C, adding them to boiled water with stirring. After complete dissolution of sodium alginate and fructose, the solutions are mixed until the required component ratios are reached, cooled to a temperature of 50-65 ° C and filtered immediately before the carbohydrate module is placed in the chamber.

In contrast to the prototype, the method for producing a biologically active additive is much simpler, shorter, and allows to reduce the cost of the target product, since it does not require preliminary separate filtration of the dietary supplement components, as well as heating the components to 120 ° C.

To obtain the claimed dietary supplement, an initial carbohydrate module is prepared. To do this, distilled water is brought to a boil in a container. Then, the right amount of water is measured and placed in a cooking tank, while the water already has a temperature below 100 ° C, the required amount of fructose is measured (assuming that the fructose content in the carbohydrate module is 10-12%), pour it in small portions with stirring in boiled water. Mixing lead to complete dissolution.

A sodium alginate solution is prepared similarly to a fructose solution. Take the right amount of boiled water from the tank and place it in the cooking tank, while the water already has a temperature below 100 ° C, measure the required amount of sodium alginate (assuming that the content of sodium alginate in the carbohydrate module is 0.7-1.5%) , pour it in small portions with stirring in boiled water. Mixing lead to complete dissolution.

It should be remembered that at temperatures above 100 ° C there is an active destruction of polymer bonds, and also not to allow the resulting solution to be outdoors for a long time in order to avoid the formation of yeast / mold, which subsequently can not only adversely affect the quality of the target product , but also significantly reduce its storage time.

The prepared solutions of fructose and sodium alginate are mixed in the amounts stated in the claims, and if necessary, boiled distilled water up to 100% is added. The prepared carbohydrate module containing sodium alginate, fructose and water is cooled to 50-65 ° C, poured into the chamber of a plastic container, passing through a filter. At the same time, the amount of carbohydrate module is poured into the chamber so that when using dietary supplement, when mixing the fat module with the carbohydrate module, the ratio (1-3.33) of the fat module to (23.33-25.67) parts of the carbohydrate module is maintained. The specified ratio of fat and carbohydrate modules allows the use of dietary supplements to obtain high-quality, non-stratified water-fat emulsion.

It was experimentally established that the temperature of the spill of the carbohydrate module of dietary supplements should not exceed 65 degrees, since this achieves safe bottling of hot solution in bottles made of glass. Lowering the temperature below 50 ° C will lead to a thickening of the gel-like solution of the carbohydrate module of dietary supplements, which will not only reduce the speed of preparation of the target product, but also lead to greater contact of the dietary supplement components with the environment, which in the end can lead to a decrease in the quality of dietary supplements and shorten its terms storage.

The use of omega-3 polyunsaturated fatty acids, eicosapentaenoic and docosahexaenoic fatty acids as a fatty module can enhance the positive properties of the carbohydrate module. This in the end result leads to the fact that the use of dietary supplements by patients helps to reduce the risk of cardiovascular disease, while ensuring a decrease in blood pressure, a decrease in blood viscosity and the risk of vascular blood clots, prevention of heart rhythm disturbances, lower cholesterol, prevention and treatment of the initial stages type II diabetes mellitus.

To obtain Pentamarine dietary supplement, you can use any container that has two separate chambers, in particular, a container protected by RF patent No. 2353345, as well as a container for pharmaceutical composition according to the RF application No. 2009138059 of 10/14/2009, can be used.

Separate storage of fat and carbohydrate modules, as well as the preparation of a dietary supplement composition immediately before use, can eliminate the disadvantages noted above, as a result of which the quality of dietary supplements increases and its shelf life increases.

The method is as follows.

The distilled water in the vessel is brought to a boil.

Example 1

Take 15 l of boiled water and fill it in a 25-liter container, then in water having a temperature below 100 ° C (by pouring it into another container), add 6000 g of fructose in small portions with stirring. Mixing lead to complete dissolution of fructose in water.

At the same time, 15 l of boiled water are taken and poured into another 25-liter container, then 420 g of sodium alginate are added in small portions to water having a temperature below 100 ° C. Stirring also leads to the complete dissolution of sodium alginate in water.

Then, the resulting solutions are poured into the cooking tank, the total volume of the solution is brought to 60 l with boiled water and stirred until a homogeneous gel-like solution of light beige color is obtained. Moreover, the resulting carbohydrate module contains, wt.%: Fructose - 10.0; sodium alginate - 0.7; water is the rest. Then the carbohydrate module is cooled to 50 ° C and poured through a filter into one of the chambers of a container made of dark colored polymer material with a capacity of 25 ml, while 23.1 g (ml) of a gel-like solution are placed in the chamber.

0.9 g (ml) of marine mammalian fat with an omega-3 polyunsaturated acid content of at least 20 wt.% Is poured into another chamber of this container. Use ready-made fat of marine mammals, corresponding to GOST 1304, TU 9281-002-88246714-11, which is fed to the workshop immediately before the filling of dietary supplements into containers. Then the container is hermetically sealed.

The ratio of fat and carbohydrate modules in the prepared Pentamarine dietary supplement is (1-25.67). Appearance of dietary supplement: in the upper chamber of a container made of dark-colored polymer material with a capacity of 25 ml there is a fat module representing marine mammals fat with an omega-3 polyunsaturated fatty acid content of at least 20%, which has a color from light orange to dark brown, and in the lower chamber the carbohydrate module in the form of a gel-like solution of light beige color. Shelf life is at least two years.

The mixture of the fat module with the carbohydrate module is carried out immediately before taking the dietary supplement by turning the lid and shaking the container, and a homogeneous sweet emulsion is obtained, which does not have extraneous odors and aftertaste.

Example 2

Take 15 liters of boiled water and fill it in a 25-liter container, then in water having a temperature below 100 ° C (by pouring it into another container), 7200 g of fructose are added in small portions with stirring. Mixing lead to complete dissolution of fructose in water.

At the same time, 15 L of boiled water are taken and poured into another 20-liter container, then 900 g of sodium alginate are added in small portions to water having a temperature below 100 ° C. Stirring also leads to the complete dissolution of sodium alginate in water.

Then, the resulting solutions are poured into the cooking tank, the total volume of the solution is brought up to 60 l with boiled water and stirred until a homogeneous gel-like solution of light gray color is obtained. The resulting carbohydrate module contains, wt.%: Fructose - 12.0; sodium alginate - 1.5; water is the rest. Then the carbohydrate module is cooled to 60 ° C and poured through a filter into one of the chambers of a container made of dark colored polymer material with a capacity of 25 ml, while 21.0 g (ml) of a gel-like solution is placed in the chamber.

3.0 g (ml) of marine mammalian fat with an omega-3 polyunsaturated acid content of at least 20 wt.% Is poured into another chamber of this container. Use ready-made fat of marine mammals, corresponding to GOST 1304, TU 9281-002-88246714-11, which is fed to the workshop immediately before the filling of dietary supplements into containers. Then the container is hermetically sealed.

The ratio of fat and carbohydrate modules in the prepared Pentamarine dietary supplement is (3.33-23.33). Appearance of dietary supplement: in the upper chamber of a container made of dark-colored polymer material with a capacity of 25 ml there is a fat module representing marine mammals fat with an omega-3 polyunsaturated fatty acid content of at least 20%, which has a color from light orange to dark brown, and in the lower chamber the carbohydrate module in the form of a gel-like solution of light gray color. Shelf life is at least two years.

The mixture of the fat module with the carbohydrate module is carried out immediately before taking the dietary supplement by turning the lid and shaking the container, and a homogeneous sweet emulsion is obtained, which does not have extraneous odors and aftertaste.

Example 3

Take 15 liters of boiled water and pour into a 25-liter container, then into water having a temperature below 100 ° C (by pouring it into another container), add 6600 g of fructose in small portions with stirring. Mixing lead to complete dissolution of fructose in water.

In parallel, 15 l of boiled water are also taken and poured into another 25-liter container, then 480 g of sodium alginate is added in small portions to water having a temperature below 100 ° C. Stirring also leads to the complete dissolution of sodium alginate in water.

Then, the resulting solutions are poured into the cooking tank, the total volume of the solution is brought to 60 l with boiled water and stirred until a homogeneous gel-like solution of light beige color is obtained. Moreover, the resulting carbohydrate module contains, wt.%: Fructose - 11.0; sodium alginate - 0.8; water is the rest. Then the carbohydrate module is cooled to 65 ° C and poured through a filter into one of the chambers of a container made of dark colored polymer material with a capacity of 25 ml, while 22.5 g (ml) of a gel-like solution is placed in the chamber.

1.5 g (ml) of marine mammalian fat with omega-3 polyunsaturated acids of at least 20 wt.% Is poured into another chamber of this container. Use ready-made fat of marine mammals, corresponding to GOST 1304, TU 9281-002-88246714-11, which is fed to the workshop immediately before the filling of dietary supplements into containers. Then the container is hermetically sealed.

The ratio of fat and carbohydrate modules in the prepared Pentamarine dietary supplement is (1.67-25.0). Appearance of dietary supplement: in the upper chamber of a container made of dark-colored polymer material with a capacity of 25 ml there is a fat module representing marine mammals fat with an omega-3 polyunsaturated fatty acid content of at least 20%, which has a color from light orange to dark brown, and in the lower chamber the carbohydrate module in the form of a gel-like solution of light beige color. Shelf life is at least two years.

The mixture of the fat module with the carbohydrate module is carried out immediately before taking the dietary supplement by turning the lid and shaking the container, and a homogeneous sweet emulsion is obtained, which does not have extraneous odors and aftertaste.

The inventive biologically active food supplement "Pentamarine" expands the range of dietary supplements and can be used for the prevention of cardiovascular diseases. The method of obtaining it in comparison with the prototype is simpler, less long, allows you to get dietary supplements with low cost and having a long shelf life.

Shelf life is 2 years. Storage conditions: at a temperature of 0 to 20 ° C in a dark place and relative humidity not more than 85%. According to microbiological indicators and the content of toxic elements, pesticides and radionuclides, the claimed dietary supplement meets the requirements of SanPiN 2.3.2.1078 (index 1.10.2), SanPiN 2.3.2.1280 (index 1.10.2).

Claims (4)

1. A biologically active food supplement containing a fat module represented by marine mammalian fat with an omega-3 polyunsaturated acid content of at least 20 wt.%, And a carbohydrate module, characterized in that it contains a fat and carbohydrate module in separate chambers, with a ratio of (1-3.33): (23.33-25.67), respectively, and as a carbohydrate module, a mixture of fructose, sodium alginate and water in the following ratio of components, wt.%:
Fructose 10-12 Sodium Alginate 0.7-1.5 Water Rest,
mixing fat and carbohydrate modules immediately before use. 2. A method of obtaining a biologically active food supplement, comprising mixing a fat module represented by marine mammalian fat with an omega-3 polyunsaturated acid content of at least 20 wt.% And a carbohydrate module, characterized in that a mixture of fructose is taken as a carbohydrate module , sodium alginate and water in the following ratio, wt.%:
Fructose 10-12 Sodium Alginate 0.7-1.5 Water Rest,
while the fat and carbohydrate modules are placed in separate chambers, with a ratio of (1-3.33) :( 23.33-25.67), and their mixing is carried out immediately before use. 3. The method according to claim 2, characterized in that the preparation of the carbohydrate module is as follows: sodium alginate and fructose are dissolved in separate containers with boiled water having a temperature of not higher than 100 ° C, adding them to boiled water with stirring, after complete dissolution sodium alginate and fructose solutions are mixed until the required proportions of the components are achieved. 4. The method according to claim 2, characterized in that before placing the carbohydrate module in the chamber it is cooled to a temperature of 50-65 ° C.