RU2431498C1 - Method of vaccination of fur-bearing animals - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to fur farming. A method involves introduction of ethane-1,2-dicarboxylic acid in the diet of fur animals. Ethane-1,2-dicarboxylic acid is introduced in the diet of fur animals for 5 days before vaccination in dose 5 mg/kg of body weight.

EFFECT: method allows improving immune status of the animals in a post-vaccination period.

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Description

The invention relates to fur farming, in particular to vaccination of fur animals against infectious diseases. This method increases the level of the immune response of animals to the administration of vaccine antigens.

A known method of vaccination, in which a single vaccination of poultry is carried out and the chemical preparation “Abactan-R” is fed at a dose of 20 mg / kg body weight 1 time per day for 14 days [1]. However, this method requires a large expenditure of the drug and its relatively long use, which is economically unjustified.

A known method of stimulating immunity in fur animals, in which vaccinated animals are injected subcutaneously for two to three days in a row at a dose of 1 ml / kg of body weight, an immunomodulator containing norstimulin and thymogen in a ratio of 1: 10000-1: 15000 [2]. However, the use of an immunomodulator according to this scheme is labor intensive and ineffective in the conditions of animal husbandry. In addition, norstimulin (T-activin) and thymogen, which are extracts of the thyroid gland of cattle, can cause allergic reactions in animals.

The closest analogue of our proposed method is a method of vaccinating a red fox [3]. It provides for the introduction into the diet of foxes of butanedioic (succinic) acid 4 days before and 4 days after vaccination at a dose of 10 mg / kg body weight, which contributes to an increase in the level of immunity factors after vaccination. However, with this method there is a large consumption of the drug and additional costs, which reduces the possibility of its widespread use in fur farms.

Our proposed method is devoid of the above disadvantages.

The purpose of the proposed method is to increase the immune status of fur animals in the period after vaccination against infectious diseases. This goal is achieved by introducing into the diet of fur-bearing animals ethane-1,2-dicarboxylic acid, which by nature is a natural metabolite of the body. It is produced in the body in small quantities and provides cell respiration processes. Ethane-1,2-dicarboxylic acid is introduced into the diet of animals within 5 days before vaccination at a dose of 5 mg / kg body weight, which helps to increase the level of immunity of animals in the post-vaccination period.

We conducted three-year studies on the use of ethane-1,2-dicarboxylic acid in the diet of fur animals in order to increase the post-vaccination immune status of animals. Specific prophylaxis against infectious diseases of a bacterial and viral nature, respectively, against salmonellosis and carnivore plague, was carried out on foxes and arctic foxes.

Example 1. From young foxes at the age of 2 months, 3 groups were formed on the principle of analog groups: one control and two experimental (16 animals each). Foxes of the 1st experimental group were included in the diet of ethane-1,2-dicarboxylic acid at a dose of 5 mg / kg body weight for 5 days before vaccination, the 2nd experimental group - for 5 days before vaccination and 3 days after vaccination. Animals of the control group did not receive the drug. Animals of all three groups were immunized with an inactivated salmonellosis vaccine according to the instructions for use. The effect of ethane-1,2-dicarboxylic acid on post-vaccination immunity was judged by the accumulation of antibodies to salmonellosis 14 days after vaccination. During this period, the fox noted the maximum production of antibodies, which were determined in the agglutination reaction. The results of the experiment are shown in table 1.

The data in table 1 show that the introduction of ethane-1,2-dicarboxylic acid in the diet of young fox helps to increase the level of post-vaccination immune response. The most effective administration of the drug within 5 days before vaccination.

Example 2. Conducted by analogy with example 1. From young foxes at the age of 2 months, 3 groups were formed according to the principle of analog groups: one control and two experimental (12 animals each). Arctic foxes of the 1st experimental group included 5 mg / kg of body weight ethane-1,2-dicarboxylic acid in the diet for 5 days before vaccination, the 2nd experimental group for 5 days before vaccination and 3 days after vaccination. Animals of the control group did not receive the drug. Animals of all three groups were immunized with the vaccine against the plague of carnivorous Bionor-D according to the instructions for use. The effect of ethane-1,2-dicarboxylic acid on post-vaccination immunity was judged by the accumulation of antibodies to the carnivorous plague virus 21 days after vaccination. In the Arctic fox during this period, the maximum number of antibodies is formed, which was determined using enzyme-linked immunosorbent assay. The results of the experiment are shown in table 2.

The data in table 2 show that the introduction of ethane-1,2-dicarboxylic acid in the diet of young fox stimulates the formation of antibodies to the carnivore plague virus, thereby increasing the immune status of animals after vaccination. The formation of post-vaccination immunity was most strongly affected by the introduction of the drug within 5 days before vaccination.

Therefore, the inclusion of ethane-1,2-dicarboxylic acid in the diet of fur animals within 5 days prior to vaccination at a dose of 5 mg / kg body weight increases the immune status of animals in the post-vaccination period.

Information sources

1. RF patent No. 2383357 C1, 03/10/2010.

2. RF patent No. 2229889 C1, 06/10/2004.

3. Bespyatykh O.Yu., Beltyukova Z.N., Berezina Yu.A. et al. The effect of butanedioic acid on the formation of post-vaccination immunity in the red fox // Actual problems of young diseases in modern conditions: Materials of an international scientific and practical conference. Voronezh: Sources, 2008. P.34-37.

Table 1 Antibody titers to salmonellosis on day 14 after vaccination of young fox Group of animals Salmonellosis antibody titer before vaccination on day 14 after vaccination Control - 1:95 1st experienced - 1: 160 2nd experienced - 1: 134

table 2 Antibody titers of the plague of carnivores on day 21 after vaccination of young fox Group of animals Carnivore plague virus antibody titer before vaccination 21 days after vaccination Control - 1: 249 1st experienced - 1: 361 2nd experienced - 1: 315

Claims (1)

A method for vaccinating fur-bearing animals against infectious diseases, comprising introducing ethane-1,2-dicarboxylic acid into their diet, characterized in that ethane-1,2-dicarboxylic acid is introduced into the diet of fur-bearing animals within 5 days prior to vaccination at a dose of 5 mg / kg body weight.