RU2412718C1 - Medication for treatment and prevention of acute respiratory viral and bacterial diseases and method of obtaining it - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of chemical-pharmaceutical and food industry, namely to field of creating liquid forms of medications and biologically active food additives, with wide spectrum of pharmacological action, namely general health-improving, anti-inflammatory, antimicrobial, antiviral and immunomodelling and antioxidant action. Claimed medication for treatment and prevention of upper airways diseases is made in form of syrup of thallus of Island moss Cetraria islandica (L.) Ach., enriched with complex of biologically active substances, including polysaccharides and lichen acids. Also claimed is method of obtaining medicinal syrup of Island moss. Vegetable raw material before extraction is preliminarily crushed and extraction is carried out with syrup of various concentrations with its further filtering.

EFFECT: method ensures obtaining of final product - syrup of Island moss, maximal substance extraction, as well as preservation of biologically active substances (BAS) in syrup (polysaccharides, lichen acids) during long term in native state, thus, ensuring high medication efficiency.

8 cl, 9 ex, 3 tbl, 3 dwg

Description

The invention relates to the pharmaceutical industry and relates to a method for producing a medicinal product and biologically active food additives from plant materials with a wide range of pharmacological effects for the prevention and treatment of acute respiratory viral and bacterial diseases.

A known drug for the prevention and treatment of tuberculosis, which has anti-inflammatory, antimicrobial, gastroprotective, immunomodulating, hepatoprotective, antiproliferative, anti-exudative and antioxidant effects, and a method for producing a drug consisting of a complex of alcoholic and aqueous extracts of Icelandic cetraria (patent RU 2203081, 2003).

The disadvantage of this invention is that the technology is multistage and involves first obtaining an aqueous extract, then an alcohol extract, combining these extracts, thickening, drying, which leads to a rise in the cost of the product, in addition, the complex extract cannot be used to treat inflammatory diseases of the oral cavity and pharynx. The disadvantage of this composition is the presence of 40-42% ethyl alcohol, which has an undesirable side effect. Such drugs are contraindicated in children.

Currently, the most common way to obtain a syrup based on medicinal plant materials is to first obtain an alcoholic or aqueous extract, and then add it to the finished sugar syrup. There is a technology for producing cough syrup (patent US 2006257507, 2006), characterized in that the extraction of crushed plant material by maceration with water for 18 hours. The resulting aqueous extract was filtered, evaporated in vacuo and dried. Next, the dry extract is dissolved in water for 18 hours, filtered and added to a pre-prepared syrup. This method involves a lengthy process, the presence of special additional extraction and evaporation equipment, which leads to a rise in the cost of technology.

The objective of the present invention is to obtain from Icelandic moss - Cetraria islandica (L.) Ach., A family of Parmelia (Parmeliaceae) water extract and obtain the finished form for internal use immediately in the form of a syrup, while ensuring the simplicity and cost-effectiveness of the process.

An agent is proposed for the treatment and prevention of acute respiratory viral infections and other diseases of the upper respiratory tract, which contains 10-70% sugar syrup of the thallus of the Icelandic tetraria (Cetraria islandica (L.) Ach.) Or a mixture of the thallus of the Icelandic tetraria (Cetraria islandica (L.) Ach .) with a medicinal plant with an expectorant, anti-inflammatory, bactericidal, antiviral and general strengthening effect when the ratio of the thallus of the Icelandic cetraria to the medicinal plant is 1:10, and a preservative in an amount of 0.2 wt.%, while the density is PA is 1.22-1.30 g / cm ³ and the mass fraction of polysaccharides is not less than 0.8%.

The preservative may be selected from the group of potassium benzoate, calcium benzoate, sodium sorbate, potassium sorbate, calcium sorbate.

The tool may further include ascorbic acid in an amount of 0.1-0.5 wt.%.

The medicinal plant can be selected from the group: thyme grass, marshmallow roots, large plantain leaves, coltsfoot leaves, sage leaves, eucalyptus leaves, chamomile flowers, rhizome and elecampane roots, anise ordinary fruits, licorice roots, succession grass, flowers linden, violet grass, St. John's wort grass, incense rhizome, blueberry fruits, rose hips, blackcurrant fruits, Echinacea purpurea grass, chicory grass.

Also, a method for producing the abovementioned agent is proposed, which consists in the fact that freshly collected or dried ground thalli of the Icelandic cetraria (Cetraria islandica (L.) Ach.) Or a mixture of the Icelandic cetraria thallus (Cetraria islandica (L.) Ach.) With a medicinal plant, possessing an expectorant, anti-inflammatory, bactericidal, antiviral and general strengthening effect when the ratio of the thallus of the Icelandic cetraria lichen to the medicinal plant is 1:10, they are extracted with 10-70% sugar syrup by maceration at a temperature 65-100 ° C for 0.5-12 hours with a ratio of the thallus of cetraria and sugar syrup 1: 10-60, then the resulting extract is filtered and a preservative is added in an amount of 0.2 wt.%, while the density of the extract is 1, 22-1.30 g / cm ³ and the mass fraction of polysaccharides is not less than 0.8%.

The preservative may be selected from the group of potassium benzoate, calcium benzoate, sodium sorbate, potassium sorbate, calcium sorbate.

For the preparation of water-sugar syrup, sorbitol and / or fructose and / or isomalt are used.

The medicinal plant can be selected from the group: thyme grass, marshmallow roots, large plantain leaves, coltsfoot leaves, sage leaves, eucalyptus leaves, chamomile flowers, rhizome and elecampane roots, anise ordinary fruits, licorice roots, succession grass, flowers linden, violet grass, St. John's wort grass, incense rhizome, blueberry fruits, rose hips, blackcurrant fruits, Echinacea purpurea grass, chicory grass.

In official and folk medicine, Icelandic cetraria (Icelandic moss, Cetraria islandica (L.) Ach.), The Parmeliaceae family (Parmeliaceae) is widely used for the treatment of acute respiratory viral infections (ARVI), other diseases of the upper respiratory tract, intestinal disorders, and dysfunctions thyroid gland, anemia, and general exhaustion [1, 2].

Icelandic moss grows in the tundra and forest zone of the European part of Russia, in the mountains of the Caucasus, Altai, Sayan and the Far East. The reserves of Icelandic cetraria are very significant. The presence of Icelandic cetraria, as a rule, is an indicator of the cleanliness of the environment [2].

As medicinal raw materials, Icelandic thalli are used, which contain biologically active substances of various groups: polysaccharides (70-80%), sugar (13%), tannins (1-2%), iridoids (2-3%), lichen acids (2-3%), trace elements, vitamins, proteins, fats, wax, gum and pigments [3].

The antitussive, antiemetic, enveloping effect of cetraria preparations is due to the presence of polysaccharides. Mucous substances of cetraria act envelopingly on the mucosa of the upper respiratory tract, therefore, they are used for bronchitis with severe cough, bronchial asthma, whooping cough, chronic bronchial catarrh. In the prescriptions of folk medicine, Icelandic mucus is combined with biologically active substances of plants such as sage, mint, coltsfoot, thyme, horsetail, angelica, calamus, serpentine. The use of fees enhances the action of each component and thereby improves the pharmacodynamics of the drug [4].

Extracts and individual compounds isolated from Icelandic cetraria in various experiments showed the following action: antioxidant [5], immunostimulating [6], antiproliferative [7] and anti-inflammatory [8].

The presence of tannins in Icelandic cetraria allows its use as an astringent and bactericidal agent in inflammatory processes of the mucous membranes, in case of burns, in case of disturbance of the gastrointestinal tract, and also as an astringent in case of poisoning with heavy metals and plant poisons.

The greatest value in Icelandic cetraria is represented by lichen acids, practically insoluble in water. Among lichen acids, usnic acid, which has a strong antibacterial effect against staphylococci, streptococci, subtilis bacteria, and mycobacteria, is of the greatest importance. Studies have shown that sodium salt of usnic acid has bacteriostatic properties at a dilution of 1: 2,000,000, kills tuberculosis bacteria at a higher concentration, and the antibiotic mechanism is associated with inhibition of oxidative phosphorylation in bacteria. Icelandic cetraria drugs, the effect of which is associated with the presence of usnic acid and its sodium salt, are used: externally - for the treatment of infectious skin diseases, inside - for the treatment of tuberculosis, whooping cough, bronchial asthma, respiratory diseases [8, 9].

Due to the high content of polysaccharides (70-80%) in combination with trace elements, Icelandic is a valuable immunomodulating agent. Icelandic moss polysaccharides have antihypoxic properties and belong to interferonostimulants and adaptogens [1, 9].

When choosing a solvent for extracting Icelandic cetraria, we were guided by the fact that the extractant should extract both the sum of water-soluble polysaccharides and water-insoluble lichen acids. In a comparative analysis of the extraction of the amount of organic acids from Icelandic cetraria, water and sugar syrup were studied as an extractant. As a result of the experiment, it was found that sugar syrup extracts 1.3 times the large amount of organic acids. These two extracts were analyzed by thin layer chromatography for the presence of usnic acid, which is of most interest among lichen acids. As a result of the detection of chromatograms in ultraviolet light, usnic acid was detected only in the extract obtained using sugar syrup as an extractant.

In the described method, Icelandic cetraria is extracted with syrup with a concentration of 10-70%, extraction is carried out by maceration in the following ratio of the cetraria thallus and syrup: 1 mass part of the cetraria thallus to 10-60 volume parts of the syrup. Extraction is carried out at a temperature of 65-100 ° C, the extraction time is 0.5-12 hours. After extraction is complete, the resulting syrup is separated from the meal by filtration or centrifugation. The resulting syrup is stabilized with a preservative in an amount of 0.2 wt.%.

As a preservative, sodium benzoate, potassium benzoate, calcium benzoate, sodium sorbate, potassium sorbate, calcium sorbate, etc. are used.

The resulting syrup is a syrupy solution of brown color, bitter taste with a pleasant aroma.

The method involves the use for extraction of any sugars, such as sucrose, sorbitol, fructose, isomaltose and others, or mixtures thereof.

Additionally, ascorbic acid in an amount of 0.1-0.5 wt.% Can be included in the composition of cetraria syrup.

Also, for the preparation of cetraria syrup, together with the thalli of cetraria, it is possible to additionally extract medicinal plant material, which has an expectorant, anti-inflammatory, antiviral, general strengthening effect. As additional components providing the required therapeutic effect, cetraria thalli can be mixed with thyme grass, marshmallow roots, large plantain leaves, coltsfoot leaves, sage leaves, eucalyptus leaves, chamomile flowers, rhizomes and elecampane roots, fruits of anise ordinary, licorice roots, succession grass, linden flowers, violet grass, St. John's wort, incense rhizomes, blueberries, rose hips, blackcurrant fruits, echinacea purpurea, chicory grass, etc., while m can optionally include both individual types of raw materials, and a mixture of raw materials.

It has been experimentally established that the technology claimed as an invention provides a preparation with the required therapeutic properties according to a simplified flow chart.

The experimental studies conducted by the authors showed that the extraction of thalli of cetraria with 10-70% syrup promotes the transfer of polysaccharides and water-insoluble organic acids into syrup by increasing the solvent properties of the extractant by introducing sugars with additional solubilizing properties into its composition.

The limits of the ratio of cetraria and syrup are selected from the conditions for obtaining such a concentration of biologically active substances of cetraria in syrup, which provides the desired therapeutic effect.

Example 1

For the preparation of cetraria syrup, freshly picked throughout the growing season or dried thallus of the Icelandic tetraria lichen Cetraria islandica (L.) Ach is used.

Prepare 10% sugar syrup, bring to a boil, remove the foam.

The crushed thallus of cetraria is loaded into the prepared hot syrup and extracted at the boil for 4 hours at a mass ratio of raw material to extractant (1:20). After extraction is complete, the resulting syrup is separated from the meal by filtration. The resulting syrup is stabilized with sodium benzoate, which is taken in an amount of 0.2 wt.%.

The main criterion for the quality of Icelandic thalli is the quantitative content of polysaccharides.

It is proposed to use the following standards as parameters for standardizing cetraria syrup: syrup density, polysaccharide mass fraction. It is proposed to determine the presence of tannins as authenticity. To quantify polysaccharides in finished form, the optical density of the products is measured in the reaction of polysaccharides with an anthron in the presence of concentrated sulfuric acid.

Example 2

Preparation of 50% isomalt syrup. Isomalt is dissolved in warm water in a ratio of 1: 1. Bring to a boil, remove the foam.

The crushed thallus of cetraria is loaded into the prepared hot syrup and extracted at the boil for 4 hours at a mass ratio of raw material to extractant (1:20). After extraction is complete, the resulting syrup is separated from the meal by filtration. The resulting syrup is stabilized with sodium benzoate, which is taken in an amount of 0.2 wt.%.

Example 3

Prepare 30% sugar syrup. Dissolve sugar in warm water, bring to a boil, remove the foam.

The crushed thallus of cetraria is loaded into the prepared hot syrup and extracted at the boil for 4 hours at a mass ratio of raw material to extractant (1:20). 3 hours after the start of the process, crushed thyme grass is added (the ratio of the thallus of Icelandic cetraria - thyme grass is 10: 1). After extraction is complete, the resulting syrup is separated from the meal by filtration. The resulting syrup is stabilized with sodium benzoate, which is taken in an amount of 0.2 wt.%.

Example 4

The cetraria syrup is prepared analogously to example 3, but instead of thyme grass or in addition to it, the following medicinal plant materials are added to the cetraria syrup: marshmallow roots, plantain leaves, coltsfoot leaves, sage leaves, eucalyptus leaves, chamomile flowers, rhizomes and elecampane roots , fruits of anise vulgaris, succession grass, linden flowers, violet grass, St. John's wort grass, incense rhizomes, blueberries, rosehips, blackcurrant fruits, purple coneflower grass, chicory grass, etc.

Example 5

Cetraria syrup is prepared analogously to examples 1, 2, 3, 4, but as sugar for the preparation of syrup use: sorbitol and / or fructose.

Example 6

Cetraria syrup is prepared similarly to examples 1, 2, 3, 4, 5, but extraction is carried out at ratios of the cetraria thallus and syrup: 1 mass part of the cetrari thallus to 10 volume parts of syrup or 1 mass part of the cetra thallus to 40 volume parts of syrup, or 1 mass part of the thallus of cetraria to 60 volume parts of syrup.

Example 7

Cetraria syrup is prepared analogously to examples 1, 2, 3, 4, 5, 6, but syrup with a concentration of 40% or 64% or 70% is used as an extractant.

Example 8

Cetraria syrup was prepared analogously to examples 1, 2, 3, 4, 5, 6, 7, but in addition, ascorbic acid in an amount of 0.1 wt.% Or 0.2 wt.%, Or 0.3 wt. %, or 0.5 wt.%.

Example 9

Cetraria syrup is prepared analogously to examples 1, 2, 3, 4, 5, 6, 7, 8, but potassium benzoate, calcium benzoate, sodium sorbate, potassium sorbate, calcium sorbate in an amount of 0.2 wt.% Are used as a preservative.

Pharmacological action of cetraria syrup.

Evaluation of the anti-inflammatory effect was carried out on a model of rat paw carrageenin edema.

The anti-inflammatory effect was antiexudative, analgesic and antipyretic. The general indicators of the inflammatory process activity were evaluated: the erythrocyte sedimentation rate, the levels of CRH, sialic acids, fibrinogen and the leukocyte content.

Figure 1-3 presents data on the anti-inflammatory activity of the studied objects.

Figure 1 shows the antiexudative effect:

syrup 1 - syrup prepared according to example 1; syrup 2 - syrup prepared according to example 1 and diluted 2 times.

Figure 2 shows the analgesic activity in the test "hot-plate test" (M ± m)):

syrup 1 - syrup prepared according to example 1; syrup 2 - syrup prepared according to example 1 and diluted 2 times.

Figure 3 shows the antipyretic effect (M ± m):

syrup 1 - syrup prepared according to example 1; syrup 2 - syrup prepared according to example 1 and diluted 2 times.

From figure 1-3 it follows that the use of cetraria syrup effectively dose-dependently has a decongestant, analgesic and antipyretic effect, while the clinical and biochemical blood parameters also normalized during treatment.

Evaluation of the antitussive effect of cetraria syrup was carried out on a model of a frog palate.

As a comparison drug used licorice root syrup. The studied drugs were used in three dilutions: 1/1000, 1/10000, 1/100000.

It was found that the cetraria syrup prepared according to example 3 in a 1/100000 dilution, corresponding to that created when taken in therapeutic doses, increases the transport function of the ciliated frog palatal epithelium.

Antimicrobial activity in relation to the reference strains of microorganisms Staphylococcus aureus ATCC 653 8-P, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Bacillus subtilis ATCC 6633, Candida albicans ATCC 885-653 and clinical isolates of microorganisms with various bacterial isolates.

The antimicrobial test results are shown in Tables 1 and 2. The minimum inhibitory concentration (MIC) of cetraria syrups with respect to test strains of microorganisms is given in table 1. The minimum inhibitory concentration of cetraria syrups with respect to clinical isolates of microorganisms is given in table 2.

Table 1 Sample MIC of cetraria syrups, having a bactericidal effect, mg / ml S.aureus ATCC 6538-P E.coli ATCC 25922 P.aeruginosa ATCC 9027 B.subtilis ATCC 6633 C.albicans ATCC 885-653 Syrup cetraria in example 1 7.8 7.8 31,2 7.8 62.5 Syrup cetraria according to example 2 15.6 15.6 62.5 15.6 125.0 Syrup cetraria according to example 3 3.9 3.9 15.6 3.9 15.6

table 2 Sample MIC of cetraria syrups, having a bactericidal effect, mg / ml S.pyogenes S. pneumoniae B.pertussis H.influenzae S.aureus Syrup cetraria, example 1 3.9 3.9 15.6 7.8 7.8 Syrup cetraria, example 2 7.8 7.8 7.8 7.8 7.8 Syrup cetraria, example 3 <3.9 <3.9 7.8 <3.9 7.8

Based on the data obtained, it was found that cetraria syrups have high bactericidal activity against test strains and clinical isolates of microorganisms. At the same time, cetraria preparations are most active in relation to the test strains Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 and Bacillus subtilis ATCC 6633, as well as to pneumococcus, which causes pneumonia, hemolytic streptococcus - the causative agent of tonsillitis, the main hemophilic bacillus - whooping cough (B.pertussis) and pustular skin lesions (S.aureus).

Antiviral activity.

Antiviral activity in in vitro experiments was evaluated against the following viruses: influenza A / Puerto Rico / 8/34 (H1N1), influenza A / Chita / 3/03 (H3N2), influenza A / Duck / Singapore / R / F 119-3 / 97 (H5N2), influenza B / Victoria / 35/72, type 5 human adenovirus (Ad5), type 3 human parainfluenza virus (PG3). Remantadin for the influenza virus and Virazole for adenovirus and parainfluenza virus were used as comparison preparations.

The antiviral activity of cetraria syrups was evaluated by lowering the titer of the virus compared to control preparations. Antiviral activity against human respiratory viruses is shown in table 3.

Table 3 Sample Concentration, mg / ml Virus titer in the presence of the drug, lg CTD50 A (H1N1) A (H3N2) A (H5N2) AT PG3 Ad5 Syrup cetraria, example 1 0.25 4.0 2.0 3.5 4.5 2.5 4.5 0.125 4.0 2.5 3.5 4.5 3.0 4.5 Syrup cetraria according to example 3 0.25 3,5 3.5 3.5 4.5 3.5 4.5 0.125 4.0 3.5 3.5 4.5 3.5 4.5 Syrup cetraria, example 2 0.25 4.0 2.5 3.0 4.5 2.5 4.5 0.125 4.0 3.0 3.5 4.5 3.5 4.5 Remantadine 0.01 3.0 1,5 1.5 4.0 but 4.5 Virazole 0.02 but* but but but 2.0 4.0 Virus control 4.0 3,5 3,5 4.5 3.5 4.5 * n / a - not determined.

As follows from the above data, comparison drugs remantadine and virazole showed the expected spectrum and level of antiviral activity. So, remantadine was active against influenza viruses A (H5N2) and A (H3N2). Virazole showed activity against parainfluenza virus.

According to the results of the studies, the following should be noted: the cetraria syrups prepared according to examples 1, 2 were active against the epidemiologically relevant subtype of influenza A (H3N2) virus and human parainfluenza virus.

Literature

1. Pamukov D.P., Akhtorjiev X.Z. Pharmacy wildlife / Per. with bulg. G.K. Smyka, N.P. Zubitskaya. - K .: Harvest, 1991 .-- 304 p.

2. Handbook of medicinal plants / A. M. Zadorozhny, A. G. Koshkin et al. - M .: Forest industry, 1990. - 412 p.

3. Safonova M.Yu., Sakanyan E.I., Lesiovskaya E.E. Cetraria islandica (L.) Ach .: chemical composition and prospects of application in medicine // Plant Resources, - 1999. - V. 35, No. 2. - S. 106-115.

4. Krishtanova N.A., Safonova M.Yu., Bolotova V.Ts. and other Prospects for the use of plant polysaccharides as therapeutic and therapeutic agents // Bulletin of Voronezh State University. Series: Chemistry. Biology. Pharmacy. - 2005. - No. 1. - S.212-221.

5. Gulcin I., Oktay M., Kufrevioglu O.I., Asian A. Determination of antioxidant activity of lichen Cetraria islandica (L) Ach. // J Ethnopharmacol. - 2002. - Vol. 79. - P.325-329.

6. Olafsdottir E.S., Ingolfsdottir K., Barsett H. et al. Immunologically active (1 → 3) - (1 → 4) -alpha-D-glucan from Cetraria islandica // Phytomedicine. - 1999. - Vol.6. - P.33-39.

7. Bucar F., Schneider I., Ogmundsdottir N., Ingolfsdottir K. Anti-proliferative lichen compounds with inhibitory activity on 12 (S) -HETE production in human platelets. // Phytomedicine. - 2004 .-- Vol.11. - P.602-606.

8. Kempe S., Gruning N., Stasche N., Honnann K. Icelandic moss lozenges in the prevention or treatment of oral mucosa irritation and dried out throat mucosa // Laryngorhinootologologie. - 1997. - Vol. 76. - P.186-188.

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Claims (8)

1. An agent for the treatment and prevention of acute respiratory viral and bacterial diseases, characterized in that it contains 10-70% sugar syrup of the thallus of the Icelandic tetraria (Cetraria islandica (L.) Ach.) Or a mixture of the thallus of the Icelandic tetraria (Cetraria islandica ( L.) Ach.) With a medicinal plant that has an expectorant, anti-inflammatory, bactericidal, antiviral and restorative effect with a mass ratio of the Icelandic and medicinal plant lichen thallus thallus 1:10 and a preservative of 0.2 wt.%, while the density of the syrup is 1.22-1.30 g / cm ³ and the mass fraction of polysaccharides is not less than 0.8%. 2. The tool according to claim 1, where the preservative is selected from the group: potassium benzoate, calcium benzoate, sodium sorbate, potassium sorbate, calcium sorbate. 3. The tool according to claim 1, further include ascorbic acid in an amount of 0.1-0.5 wt.%. 4. The tool according to claim 1, where the medicinal plant is selected from the group: thyme grass, marshmallow roots, large plantain leaves, coltsfoot leaves, sage leaves, eucalyptus leaves, chamomile flowers, rhizome and elecampane roots, fruits of anise ordinary, licorice roots, succession grass, linden flowers, violet grass, St. John's wort, incense rhizome, blueberry fruits, rose hips, blackcurrant fruit, purple coneflower herb, chicory herb. 5. The method of obtaining funds according to claim 1, characterized in that the freshly collected or dried crushed thalli of the Icelandic tetraria (Cetraria islandica (L.) Ach.) Or a mixture of the Icelandic cetraria thallus (Cetraria islandica (L.) Ach.) With a medicinal a plant with an expectorant, anti-inflammatory, bactericidal, antiviral and general strengthening effect with a mass ratio of the Icelandic and medicinal plants lichen thallus mass 1:10 is extracted with 10-70% sugar syrup by maceration at a temperature of 65-100 ° С in those 0.5-12 hours at a ratio of the thallus of cetraria and sugar syrup 1: 10-60, then the extract obtained is filtered and a preservative is added in an amount of 0.2 wt.%, while the density of the extract is 1.22-1.30 g / cm ³ and the mass fraction of polysaccharides is not less than 0.8%. 6. The method according to claim 4, where a preservative selected from the group of potassium benzoate, calcium benzoate, sodium sorbate, potassium sorbate, calcium sorbate is used. 7. The method according to claim 4, where sorbitol and / or fructose and / or isomalt are used to prepare the water-sugar extract. 8. The method according to claim 4, where a medicinal plant selected from the group is used: thyme grass, marshmallow roots, large plantain leaves, coltsfoot leaves, sage leaves, eucalyptus leaves, chamomile flowers, rhizome and elecampane roots, anise fruits common, licorice roots, string grass, linden flowers, violet grass, St. John's wort grass, incense rhizome, blueberry fruits, rose hips, blackcurrant fruits, Echinacea purpurea grass, chicory grass.

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