RU2412702C1 - Composite preparation for prevention and treatment of gastroenteritis in piglets - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary. A composite preparation for prevention and treatment of gastroenteritis of piglets contains the active agent tylosin tartrate and the synergist furasonal in the ratio 3:1. For prevention of gastroenteritis in piglets, the preparation is administered in dosage of tylosin tartrate 3.75 mg/kg of live body weight and furasonal 1.25 mg/kg of live body weight for 7 days, for treatment of piglets with gastroenteritis, the preparation is administered in dosage of tylosin tartrate 7.5 mg/kg of live body weight and furasonal 2.5 mg/kg of live body weight for 10 days.

EFFECT: invention provides higher therapeutic and preventive efficacy of the preparation and prevention of tolerance thereto by piglets' gastroenteritis agents.

3 cl, 5 tbl

Description

The present invention relates to veterinary medicine and can be used for the prevention and treatment of piglet gastroenteritis.

There are various antibacterial agents used to treat gastroenteritis in piglets: tetracyclines, oxamycins, macrolides and fluoroquinolones, as well as immunoglobulin-C [Use of immunoglobulin-C allogeneic for the treatment of gastrointestinal diseases of piglets / A.G. Shakhov, V.I. Lesnykh, E.G. Pozhenko, Yu.N. Brigadirov, S.I. Chaika, N.P. Zuev. - Voronezh, 1990. - 4 p. - (IL / Voronezh Central Scientific Research Institute, No. 362-90)].

In connection with the spread of infectious diseases caused by pathogens of not only bacterial but also viral nature, compositional preparations containing antimicrobial and antiviral components [RU 2118529 C1, A61K 31/00, 09/10/1998: Veterinary drug “Tricaptol” for the treatment of gastroenteritis are spreading young s.kh. animals].

For the prevention of gastroenteritis in piglets, it is recommended to use vitamin A, E, C and cyanocobalamin preparations [Sevryuk I.Z. Prevention of immune deficiencies and diarrhea in young animals / I.Z.Sevryuk // Ecological problems of pathology, pharmacology and animal therapy: International. coordination meeting. - Voronezh, 1997. - S. 346-348]. Widespread use of the composition of nitazole, sulfonamides and antibiotics [Shabunin S.V. / Therapy and prevention of gastrointestinal diseases of bacterial etiology in piglets with combinations of nitazole with sulfanilamides and antibiotics / S.V.Shabunin, P.A. Parshin // Ecological problems of pathology, pharmacology and animal therapy: International coordination meeting. 1997. - P.369].

Fradizin and tylosin tartrate have therapeutic efficacy in piglet gastroenteritis [SU 681598 A1, A61K 31/00, A61P 31/00, 03/23/1981; Antipov V.A. Antibiotic technical preparations - effective therapeutic and prophylactic agents for veterinary medicine and animal husbandry / V.A. Antipov, B. C. Grishkevich // Collection of scientific works “Pharmacology and toxicology of new drugs and feed additives in veterinary medicine”. - Leningrad, 1990. - S.171-173; Antipov V.A. The drug for the treatment and prevention of gastroenteritis and bronchopneumonia of pigs / V.A. Antipov, A.G. Shakhov // Certificate for the rat. proposal GUV GAPK of the USSR dated 06/10/1986. - No. 439-11 / 2015].

A known method for the prevention and treatment of infectious diseases of pigs using fradizin - macrolide antibiotic [Description of the invention to the copyright certificate SU 681598 A1, A61K 31/00, A61P 31/00 / OA Gavrilova, AM Makukhina, A. G. Rezvykh and others ., 1981], which relates to the closest analogue of the claimed invention.

However, the use of fradizin for the prevention of infectious diseases of pigs and the treatment of sick animals requires its daily administration with feed in an amount of 0.2-0.4 kg per 1 ton for prevention and 0.4-4.0 kg per 1 ton for treatment, respectively.

Tylosin tartrate is one of the drugs of microbiological synthesis. Compared with fradizin, it has a higher antimicrobial activity in relation to gram-positive and gram-negative microflora [Bukhanov V.D. Therapy and prevention of pig dysentery / V.D. Bukhanov // Abstract. dis ... cand. Vet.n., Moscow, 1986.- 24 p .; Zuev N.P. Obtaining and development of antimicrobial compositions based on tylosin-containing preparations / N.P. Zuev, V.D. Bukhanov // Materials of the First Congress of Veterinary Pharmacologists of Russia, June 21-23, 2007. - RAAS VNIVIPFiT, Voronezh, 2007. - S.311-316].

The disadvantages of tylosin-containing drugs are: a narrow spectrum of activity against microorganisms that cause gastroenteritis of piglets, the rapid emergence of pathogens resistance to them.

Object of the invention: increasing the therapeutic activity of tylosin tartrate in the treatment of piglets with gastroenteritis of bacterial etiology.

The solution of this problem allows you to:

1. The expansion of the spectrum of antimicrobial action of drugs in severe infections against microbes that cause gastroenteritis in piglets requiring immediate treatment before a bacteriological diagnosis is established, or in mixed infections, when microbial pathogens have different sensitivity to antibacterial drugs.

2. To prevent the development of toxic effects by achieving a quicker and more complete effect with the simultaneous administration of two or more drugs in lower than usual, course doses. This is of great importance when using antibiotics that cause nephrotoxic (aminoglycosides, polymyxin, griseofulvin), hepatotoxic (tetracycline, erythromycin, novobicin) actions, an adverse effect on the gastrointestinal tract (tetracyclines, erythromycin, griseofulvin) and the blood-forming system.

3. Potentiation of the antibacterial effect.

4. Prevention or reduction of the occurrence of acquired drug resistance of pathogenic microorganisms.

Based on the above requirements, macrolide and nitrofuran drugs are promising for the creation of composite antibacterial agents with a wide spectrum of action. In addition, combinations with nitrofurans have the ability to inhibit R plasmids of resistance of microorganisms to pharmacological agents.

Tylosin-containing preparations have high antimicrobial activity in relation to gram-positive and relatively low - to gram-negative microflora. Nitrofuran compounds exhibit a wide range of antimicrobial activity [Nitrofurans / I.E. Brains // Pharmacology. - M .: Agropromizdat, 1985. - P.338-341; Bukhanov V.D. Therapy and prevention of pig dysentery / V.D. Bukhanov // Abstract. dis ... cand. Vet.n., Moscow, 1986.- 24 p .; Zuev N.P. Obtaining and development of antimicrobial compositions based on tylosin-containing preparations / N.P. Zuev, V.D. Bukhanov // Materials of the First Congress of Veterinary Pharmacologists of Russia, June 21-23, 2007. - RAAS VNIVIPFiT, Voronezh, 2007. - S.311-316].

The synergistic combination of tylosin tartrate with furazonal in a 3: 1 ratio is effective in treating sick animals and preventing the development of gastroenteritis in piglets. This combination is effective when administered with food for the prevention of gastroenteritis at a dose of tylosin tartrate 3.75 mg / kg body weight and furazonal 1.25 mg / kg body weight for 7 days, and for the treatment of piglets with gastroenteritis at a dose of tylosin tartrate 7.5 mg / kg body weight and furazonal 2.5 mg / kg body weight for 10 days.

Examples of specific performance.

Laboratory studies allow us to determine the optimal ratio of the components in the composite composition being created when studying their antimicrobial activity against bacteria that play an etiological role in the occurrence of piglet gastroenteritis. The research results are presented in table 1.

Table 1
Antimicrobial activity of the composition of tylosin tartrate with furazonal Type of microorganisms Escherichia coli Salmonella cholerae suis Brachyspira hyodysenteriae Minimum bacteriostatic concentration (MBC), mcg / ml: tylosin tartrate 17 fifty 26 furazonal 10 10 0.6 compositions of tylosin tartrate with furazonal 1: 1 8.0 8.0 9,4 2: 1 6.0 6.0 8.3 3: 1 5,0 5,0 6.3 Fractional inhibitory concentration (μg / ml) in a ratio of 3: 1 tylosin tartrate 0.2 0.1 0.2 furazonal 0.1 0.1 0.1 FIC index of the connection 0.3 0.2 0.3

As the data in this table show, the most effective proportion of the constituents of the composite preparation, which potentiates the antimicrobial activity of the combination, is a 3: 1 ratio of tylosin tartrate and furazonal.

The fractional inhibitory concentration (FIC) of tylosin tartrate in relation to E. coli, S.ch.suis, Brachyspira hyodysenteriae when interacting with furazonal was 0.2; 0.1; 0.2 μg / ml, furazonal - 0.1; 0.1 and 0.1; and of a composite compound, 0.3; 0.2; 0.3 μg / ml, respectively.

table 2
Prevention of adaptation of microorganisms to pathogens of gastroenteritis in piglets Bacteriostatic concentration of tylosin tartrate, μg / ml of culture medium Background (MBK after the first passage) After 30 passages on culture media containing furazonal E. coli Salmonella Brahispira E. coli Salmonella Brahispira 17 fifty 26 17 fifty 26

From the data of table 2 it can be seen that the antimicrobial activity of tylosin tartrate against pathogens of gastroenteritis in piglets does not change even after 30 passages, which indicates the inhibition of resistance plasmids in the studied microorganisms using furazonal.

Table 3
Comparative prophylactic efficacy of drugs for gastroenteritis in piglets Group of animals Compositional drug Positive control The control Tylosin tartrate Furazonal The number of piglets at the beginning of the experiment, goal. twenty twenty twenty twenty Piglets got gastroenteritis, goal. four 5 5 8 Preventive efficacy,% 80 75 75 -

The results of an experiment on the prophylactic effect of a composite preparation at a dose of tylosin tartrate 3.75 mg / kg body weight and furazonal 1.25 mg / kg body weight (animals received the drug with food for 7 days) are presented in table 3.

In a production experiment to test the prophylactic effectiveness of the developed composition of tylosin tartrate in combination with furazonal for piglet gastroenteritis, 500 conditionally healthy animals were used, which were divided into two groups (Table 4).

Table 4
Comparative prophylactic efficacy of tylosin and furazonal in piglet gastroenteritis Indicators Groups of animals Experienced Control The number of animals at the beginning of the experiment, goal. 350 150 The average body weight of one animal at the beginning of the experiment, kg fifteen fifteen The average body weight of the animal at the end of the experiment, kg 17.6 17.0 The average daily weight gain, g 260 200 There were healthy animals at the end of the experiment, a goal. 335 132 Preventive efficacy,% 95 -

Animals of the first group (n = 350) for 7 days were fed with tylosin tartrate along with furazonal at a dose of tylosin tartrate 3.75 mg / kg body weight and furazonal 1.25 mg / kg body weight, piglets of the second (n = 150 goals.) - the drug was not prescribed (control).

Piglets were monitored for 30 days, during which the incidence and growth rate were taken into account.

The results of the studies (table 4) show that the preventive effect of the proposed composition was 74% (with an incidence of 32% in the control), the average daily growth rate of animals in the 1st group was 260 g, and in the control 200 g.

The results of the study of the therapeutic effectiveness of the composite preparation for gastroenteritis of piglets are presented in table 5.

Table 5
Comparative therapeutic efficacy in diseases of young animals Group Tylosin tartrate and furazonal Tylosin tartrate Furazonal The number of sick animals at the beginning of the experiment, goal. fifteen fifteen fifteen The number of animals recovered by the end of the experiment, goal. 12 eleven 10

From the data of table 5 it can be seen that the therapeutic efficacy of the composition of tylosin tartrate and furazonal when given with food for 10 days at a dose of tylosin tartrate 7.5 mg / kg body weight and furazonal 2.5 mg / kg body weight is higher than the original drugs separately.

The data obtained indicate that the prophylactic and therapeutic effectiveness of a composite preparation increases due to the synergistic interaction of tylosin tartrate and furazonal, as well as the prevention of the formation of resistance to these drugs in microorganisms - causative agents of gastroenteritis.

The present invention is a composite preparation for the prevention and treatment of piglet gastroenteritis by administering to the animals a composition of tylosin tartrate and furazonal in a ratio of 3: 1. The composite preparation is effective for piglets from 2 weeks to 4 months of age. The drug is effective at a dose of tylosin tartrate 7.5 mg / kg body weight and furazonal 2.5 mg / kg body weight of piglets for 10 days with treatment and tylosin tartrate 3.75 mg / kg body weight and furazonal 1.25 mg / kg body weight for 7 days in the prevention of gastroenteritis. The introduction of a composite preparation can begin when the pigs show the first signs of gastroenteritis or 2-5 days before exposure to stresses arising from technological situations (regrouping, weighing, vaccination, etc.), which are one of the causes of gastroenteritis.

The use with feed of a developed and tested composite preparation is the most technologically advanced and economically feasible way.

Claims (3)

1. A composite preparation for the prevention and treatment of piglet gastroenteritis, including the active principle of tylosin tartrate and synergist furazonal in a ratio of 3: 1. 2. The composite preparation according to claim 1, characterized in that for the prevention of gastroenteritis in piglets it is administered at a dose of tylosin tartrate 3.75 mg / kg body weight and furazonal 1.25 mg / kg body weight for 7 days. 3. The composite preparation according to claim 1, characterized in that for the treatment of piglets suffering from gastroenteritis it is administered at a dose of tylosin tartrate 7.5 mg / kg body weight and furazonal 2.5 mg / kg body weight for 10 days.