RU2399051C1 - Method for estimating dynamic balance of human homeostasis stability - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: pH and Redox values of the potential of biological fluids, namely urine and saliva are recorded. A diagram

is plotted, and the antiphase fluctuations of varying measured parametres on the timing chart are used to transform the diagram in a histogram wherein the height of each columns is proportional to the area of diagram between the proximate measurements, respectively. An absolute value of total area of the positive columns and total area of the negative columns is determined, and if they are compared to their average value to exceed no more than by 15% thereof, and differ no more than by 15 % thereof, dynamic balance of homeostasis stability is considered to be normal within limits, and if they exceed 15% of the value, then they are considered to be subnormal.

EFFECT: higher estimation accuracy.

2 cl, 26 dwg

Description

The invention relates to the field of biophysics, experimental and clinical medicine, in particular to methods for diagnosing a functional state of a person, and can be used for integral assessment of a person's condition in the diagnosis and correction of pathological conditions of the body.

Known methods for diagnosing the functional state of a biological object by separately measuring the pH of various liquid media (for example, human urine) of the body of a biological object (see E. Revich. "Research in physiopathology as the basis of controlled chemotherapy in relation to cancer, New York, 1961) or by measuring separately the Redox potential (see AS USSR No. 602860, class A61B 5/00, 1975).

However, a separate measurement of the above parameters of the liquid media of a biological object and an absolute value assessment without taking into account changes in parameters over time does not provide a qualitative and quantitative assessment of the state of the biological object, which reduces the reliability and reliability of the diagnosis.

There is a method for diagnosing the functional state of a biological object, in which the Redox potential (ORP) of the liquid medium of the object under study is measured and the obtained values are compared with a health standard, after which it is estimated how the value of the Redox potential correlates with the health of the object (see US patent No. 6269261, class A61B 5/05, 2001).

However, this method does not allow to give a qualitative and quantitative diagnosis of the state of the bioobject, as they measure and evaluate the state of the bioobject only by the absolute value of one parameter - the Redox potential. In addition, the vibrational nature of the change in the measured parameter over time is not taken into account.

по колебаниям значений вариаций измеряемых параметров на их временной диаграмме, преобразуют его в столбчатую диаграмму, где высота каждого из столбцов пропорциональна площади графика между соседними измерениями соответственно, и по отношению суммы площадей положительных столбцов к сумме площадей отрицательных столбцов за фиксированный интервал времени, в качестве которого могут быть приняты значения от минутного до годового, судят о состоянии биообъекта, причем за норму считают значение, не выходящее из диапазона 0,5÷2 (см. пат. РФ №2258227).By technical essence, the closest to the present invention is a method for diagnosing the functional state of a biological object, in which the pH and Redox values of the potential of the liquid media of biological objects are recorded at the same time, a graph is built

according to the fluctuations in the values of the variations of the measured parameters on their time chart, they transform it into a bar chart, where the height of each column is proportional to the area of the graph between adjacent measurements, respectively, and the ratio of the sum of the areas of the positive columns to the sum of the areas of the negative columns for a fixed time interval, in which values can be taken from minute to annual, judge the state of the bioobject, and consider the norm to be a value that does not fall outside the range of 0.5 ÷ 2 (see Pat. RF №2258 227).

However, the known method does not allow to sufficiently determine the real adaptive capabilities of a person, since the known method does not determine and analyze the dynamic balance of the stability of homeostasis of a living organism, since there is no process of simultaneously measuring the pH and Redox potential of urine and saliva, and they only measure simultaneously of these parameters for only one type of liquid medium: urine or saliva. This reduces the quality of the diagnostics of the process of assessing the adaptive capabilities of a person.

The technical result is to improve the quality of diagnosis of a person’s condition by providing an analysis of the dynamic balance of the stability of his homeostasis while recording the pH and Redox potential for urine and saliva.

и по противофазным колебаниям значений вариаций измеряемых параметров на их временной диаграмме преобразуют его в столбчатую диаграмму, где высота каждого из столбцов пропорциональна площади графика между соседними измерениями соответственно, определяют абсолютное значение сумм площадей положительных столбцов и сумм площадей отрицательных столбцов и при превышении их значения по сравнению с их среднестатистическим значением не более чем на 15% от его величины и при их разнице между собой, не превышающей 15% от его величины, считают, что адаптационные возможности исследуемого человека в пределах нормы, а при превышении 15% значения - ниже нормы, причем регистрацию значений рН и Redox потенциала для мочи и слюны производят одновременно, кроме того, в качестве среднестатистического значения сумм площадей положительных и отрицательных столбцов берут значение 0,75±0,25.This is achieved by the fact that in the method for determining the adaptive capacity of a person according to the invention, the pH and Redox values of the potential of the living body fluids are recorded, using urine and saliva as a graph.

and from the out-of-phase fluctuations of the values of the variations of the measured parameters on their time chart, they transform it into a bar chart, where the height of each of the columns is proportional to the area of the graph between adjacent measurements, respectively, the absolute value of the sums of the areas of the positive columns and the sums of the areas of the negative columns is determined and when their values are exceeded in comparison with their average value not more than 15% of its value and with their difference between themselves not exceeding 15% of its value, it is believed that the adaptive capabilities of the person under investigation are within normal limits, and when exceeding 15%, they are below normal, and the pH and Redox potential for urine and saliva are recorded simultaneously, in addition, 0.75 is taken as the average statistical value of the sum of the areas of positive and negative columns ± 0.25.

The essence of the invention lies in the fact that the above operations of the method provide a qualitative analysis of the dynamic balance of stability of the homeostasis of the human body and increase the accuracy of the assessment - determine the adaptive capabilities of the person, since pH and Redox potentials for saliva and urine are measured simultaneously. Therefore, vibrations that provide dynamic acid-base and redox balances are not only interconnected, but also occur in antiphase to each other.

Comparison of the proposed method with the closest analogue allows us to claim compliance with the criterion of "novelty", and the absence of distinctive features in the analogs indicates compliance with the criterion of "inventive step".

Preliminary tests confirm the possibility of widespread clinical use.

и

соответственно для здорового человека, а на фиг.3в и 3г - для больного; на фиг.4а и 4б - графики

- столбчатая диаграмма площадей за фиксированный промежуток времени (за сутки) здорового, а на фиг. 4в и 4г - больного; на фиг.5а и 5б - графики изменения отношения сумм площадей S(+)/S(-) положительных и отрицательных столбцов за месяц (для нормы и выходящий за пределы нормы соответственно), на фиг.6а и 6б - графики

и

для мочи и слюны одновременно (здорового), а 6в и 6г - больного; на фиг.7а и 7б - график Redox потенциала и рН одновременно для мочи и слюны здорового, а на фиг.7в и 7г - больного; на фиг.8а и 8б - график Redox потенциала и рН для здорового и больного, и на фиг.9 - временные диаграммы измеряемых

мочи и слюны здорового и больного.Figure 1 presents a functional diagram of a device for implementing the proposed method, figure 2A and 2B shows the timing diagrams of the measured Redox potential and pH for a healthy person, and in figures 2B and 2g for a patient; on figa and 3b presents graphs

and

respectively for a healthy person, and on figv and 3d for the patient; on figa and 4b - graphs

- a bar graph of areas for a fixed period of time (per day) healthy, and in FIG. 4c and 4g - of the patient; on figa and 5b are graphs of changes in the ratio of the sums of areas S (+) / S (-) of positive and negative columns per month (for the norm and beyond the norm, respectively), on figa and 6b are graphs

and

for urine and saliva at the same time (healthy), and 6c and 6g - for the patient; on figa and 7b is a graph of the Redox potential and pH simultaneously for healthy urine and saliva, and on figv and 7g - patient; on figa and 8b is a graph of Redox potential and pH for healthy and sick, and Fig.9 is a timing chart of measured

urine and saliva healthy and sick.

A device for implementing the proposed method comprises an electrode system 1, a converter 2, a microprocessor unit 3 and a display 4.

The electrode system 1 contains three electrodes, two of which are designed to measure pH and Redox potential, respectively, and one for comparison. In addition, the electrode system can be equipped with a container (cuvette) for placement of the studied biological fluid, such as urine.

It should be noted that in the study of other human biological fluids, such as saliva, it is not necessary to use a container, but it is necessary and sufficient to place electrodes, made, for example, in the form of probes, in the mouth. At the same time, the potential difference between each of the electrodes and the reference electrode is measured and fixed on a computer display.

и

(фиг.3а и 3б) для здорового и (фиг.3в и 3г) для больного, которые являются вариациями измеряемых параметров на интервале Δt (ч), который является интервалом между последовательными измерениями.In this case, the pH and Redox potential are recorded simultaneously for saliva and urine, which is reflected in FIGS. 2a and 2b (for healthy) and in FIGS. 2c and 2g (for the patient). After that build

and

(figa and 3b) for healthy and (figv and 3d) for the patient, which are variations of the measured parameters in the interval Δt (h), which is the interval between consecutive measurements.

на каждом временном интервале измерений Δt (ч), преобразуя его в столбчатую диаграмму (фиг.4а для мочи и фиг.4б для слюны по суткам) для здорового и (фиг.4в для мочи и фиг.4г для слюны по суткам) для больного соответственно, где высота каждого из столбцов пропорциональна площади графика между соседними измерениями соответственно.Next, they build a graph

at each time interval of measurements Δt (h), converting it to a bar graph (Fig. 4a for urine and Fig. 4b for saliva daily) for healthy and (Fig. 4c for urine and Fig. 4g for saliva daily) for the patient respectively, where the height of each of the columns is proportional to the area of the graph between adjacent measurements, respectively.

Further, according to the ratio of the area of the positive columns to the sum of the areas of the negative columns for a fixed time interval S (+) / S (-), which can be taken as the values from minute to annual, the state of the bioobject is judged, and the values considered are not normal out of the range of 0.75 ÷ 0.25, it is believed that the greater the absolute values of the sums of the areas of positive and negative columns, the better the condition of the bioobject.

At the same time, in some individuals, the absolute values of both pH and Redox potential may differ, but the relative fluctuations of the measured values must remain in dynamic equilibrium.

It should be noted that all measured values are recorded and processed automatically according to the corresponding program in microprocessor unit 3 and are observed on display 4.

The relationship of living things with the environment as a single open system is a fundamental problem in biology. Nature solved the problem of adaptation to an ever-changing environment by building a different hierarchy of rhythms of living beings, which to a different degree resonant with the rhythms of the environment and restoring dynamic equilibrium with it in different ways. Therefore, the proposed criteria for evaluating the described two-phase process with higher sensitivity will be able to show the imbalance parameters in a living system and correct it in time.

In light of the foregoing, we consider it appropriate to simultaneously measure the pH and ORP of saliva and urine. The pH and ORP measurements of saliva in a healthy patient are presented in FIGS. 2a and 2b, and in a patient in FIGS. 2c and 2d. From the presented it can be seen that the observed two-phase systemic process can be observed as an alternation of redox processes that occur alternately and are natural vibrations that provide a general dynamic balance of the stability of homeostasis. In this case, the dynamic acid-base and redox balances are interconnected.

The absolute values of both pH and ORP in a healthy person fluctuate in a wider range of values (pH from 6.1 to 7.2; ORP from -30 mB to +250 mB) than in a patient (pH from 7.2 to 7 , 5; ORP from +10 mB to +125 mB), i.e. the limit of fluctuations in the first case is for pH 0.9 units, and for ORP 280 mV, and in the second, respectively, for pH 0.3 units, and for ORP 115 mV. In the second case, there is a kind of extinction of the oscillatory process, because the greater the difference in the measured values (up to a certain limit), the greater the degree of stability of homeostasis.

Therefore, only the proposed method allows us to provide a qualitative and quantitative diagnosis of the functional state of a biological object and expand the functionality of assessing the imbalance of the body, since without comparing simultaneously measured indicators between different environments, it was not possible to really assess the acceptable characteristics of transients to determine safe boundaries of adaptation to external disturbances.

Thus, in the proposed technical solution, the delivered technical result is achieved.

Claims (2)

1. A method for assessing the dynamic balance of stability of human homeostasis, characterized in that the pH and Redox values of the potential of the living organism’s body fluids are recorded, using urine and saliva as a graph.

and from the out-of-phase fluctuations of the values of the variations of the measured parameters on their time chart, they transform it into a bar chart, where the height of each of the columns is proportional to the area of the graph between adjacent measurements, respectively, determine the absolute value of the sums of the areas of the positive columns and the sums of the areas of the negative columns and compared with their average value, not more than 15% of its value, and with their difference between themselves, not exceeding 15% of its value, consider a dynamic balance homeostasis stability test person within the normal range, and when exceeding 15% below the normal value is considered, the recording of pH and Redox potential for urine and saliva are performed simultaneously. 2. The method according to claim 1, characterized in that the average value of the sum of the areas of the positive and negative columns is taken to be 0.75 ± 0.25.

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