RU2386132C2 - Agent for indication of potential propensity for disease in cats (versions) and method for determining health situation in cats - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: agent for indication of potential propensity for disease in cats represents indicator granules including a base and pH indicator. As a base, white granulated perlite of fraction 1 to 6 mm and content 96 to 99% in an end product. Said pH indicator is methyl red or alizarin or bromine cresol purple or bromine thymol blue or phenol red or mixtures.

EFFECT: invention allows for rapid and easy detection of signs of a potential disease.

3 cl, 3 tbl, 12 ex

Description

The invention relates to veterinary medicine, in particular, to a method for detecting cat health problems at home. The invention is intended for the prevention (timely detection) of cat health disorders (prerequisites for the occurrence of a urological syndrome, urolithiasis, etc.) at home through the use of indicator granules. At the same time, the product is not a veterinary drug, but serves as a simple rapid test, the purpose of which is to promptly notify the owner of the animal about the need to visit the veterinarian.

At the present stage of development of veterinary medicine, the urgent issue of identifying a disease in cats in an outpatient setting using express methods. If various symptoms suggest the appearance of a particular disease, then various methods for determining the status of a cat are used for diagnosis. In particular, they use X-ray examination of the kidneys, ureters and bladder, as well as a simple ultrasound examination. A urine test for calcium, phosphorus and uric acid is also used. The urine of clinically healthy animals has an acid reaction (pH 6-6.5), protein is absent or occurs in trace amounts and the specific gravity of urine does not exceed 1.02. These methods can only be used in a veterinary clinic, since they require significant energy costs (X-ray unit and ultrasound) or the expense of expensive reagents.

For pet cat owners, this issue is relevant, since the clinic’s cost recovery for conducting comprehensive studies of the health status of a pet rests with the owner of the animal. Therefore, it was important to create a tool for rapid diagnosis of health problems in cats at home. This problem was solved by selecting a set of indicators that, with a high degree of probability, can show the presence (occurrence) of a disease state in an animal. It is a known fact for a specialist that under normal conditions of an animal’s health, urine pH is in the range of 6.0-6.5 (slightly acidic environment), and when, for example, an urolithiasis occurs in an animal, the urine pH “moves” to the alkaline region 7, 0-9.2 (the last value with very severe kidney damage).

As the closest analogue can be used the technical solution presented in the document US 6376252, publ. 04/23/2002. This patent describes an indicator system for determining the presence of blood in the urine of cats, which may indicate a late stage of development of urolithiasis in cats, since urinary stone damages the tissues of the kidney and urinary tract using expensive reagents. The system contains a matrix including a benzidine derivative, cumene hydroperoxide or diisopropylbenzene hydroperoxide, and a buffer with a pH of 6.0 and a surfactant based on sodium lauryl sulfate are used in the system. Such a system is expensive and time consuming to obtain.

It is known from RU 2304778, publ. 08/20/2007, a tool for rapid diagnosis of urolithiasis in cats, which is indicator granules containing the following components, g:

Alizarin 0.45 Bromphenol red 0.40 Potato starch 180 Fine Chalk one hundred Millet 550 Starch paste fifty

In the known patent, in contrast to the previously described analogue, readily available components are used, which can be obtained (manufactured) in a rather simple way. The use of indicator granules allows you to identify the disease (urolithiasis) in the initial stages, when the disease has not yet manifested itself.

However, these granules in their composition are designed for rapid diagnosis of only such disorders, a qualitative assessment of which is a change in the color of the indicator in the pH range of 8.5-10.2. At the same time, such areas as pH, from 6.0 to 8.5 and below 6.0, are not affected.

The present invention is aimed at solving the technical problem of creating granules of indicators with an extended range of qualitative assessment of changes in the acidity of cats urine, which allows for a substantially new approach to the prevention and detection of the initial stages of cat diseases. At the same time, the task of expanding the range of components on the basis of which indicator granules can be obtained while maintaining their functionality in terms of acidity indication is also solved.

The technical result achieved by the present invention is to increase the reliability of information about the initial stages of cat diseases by expanding the range of quality control over changes in urine acidity. In addition, the result lies in the possibility of producing indicator granules using a wide range of modern substances.

The specified technical result in terms of the device is achieved by the fact that in the tool for indicating the potential predisposition of cats to the disease, which is indicator granules, which include the base and indicator, white perlite granular fraction from 1 to 6 mm with a content of from 96 to 99% in the composition of the finished product, and the indicator in the form of an aqueous solution includes a pH level indicator and a surfactant, while methyl red, silt was used as an indicator of the pH level alizarin or bromocresols magenta, or bromotimol blue or phenol red, or mixtures thereof, as well as the surfactant used polyvinyl alcohol or polyvinyl ether, or polyvinyl pyrrolidone, or polymethacrylamide, or Polyoxymethylene, or polyethylene oxide, or polymethacrylic acid.

The specified technical result in terms of the device is achieved by the fact that in the means for indicating the potential predisposition of cats to the disease, which is a set of indicator granules, including a base and an indicator, characterized in that the basis of the indicator granules use perlite white granular fraction from 1 to 6 mm with a content of 96 to 99% in the composition of the finished product, and the indicator in the form of an aqueous solution includes a pH level indicator and a surfactant, while for some of the aboriginal granules in the kit, methyl red, or alizarin, or purple bromocresol, or mixtures thereof was used as an indicator of the pH level; for the other part of these granules in the kit, bromotimol blue, or phenol red, or mixtures thereof was used, and as a surfactant polyvinyl alcohol or polyvinyl methyl ether or polyvinyl pyrrolidone or polymethacrylamide or polyoxymethylene or polyethylene oxide or polymethacrylic acid was used.

The specified technical result in terms of the method is achieved by the fact that in the method for determining the health problems of cats, which consists in adding granules of indicators to the cat litter that change color at a pH of more than 6.5, and recording color changes of these granules of indicators when they get on them cat urine, additionally use indicator granules, which, when cat urine gets on them, change color when pH is more than 5.0 and less than 6.0 and more than 6.5 and less than 9.2, and cat's health is judged by changing the color of the granules in dictators relative to their original color.

These features are significant and are interconnected with the formation of a stable set of essential features sufficient to obtain the desired technical result.

According to the present invention, a product is considered - indicator granules intended for the prevention (timely detection) of cat health disorders (prerequisites for the occurrence of a urological syndrome, urolithiasis, etc.) at home.

The product is a round-shaped granules from white and yellow to pale orange, bulk density of about 150 g / l, very fragile. Indicator granules are poured on top of any toilet filler. Within 15-30 seconds after the urine enters the granules, a reaction occurs showing the degree of potential predisposition to the disease by changing the color of the granules. The results of the reaction are clearly visible for several hours, then the color gradually weakens due to evaporation. Granules can be made both with a separate indication of a specific range of urine acidity, and in the form of a set of granules for indicating urine acidity in the entire measurement range.

These granules consist of a base and additional components, the total share of which is up to 1% by volume of the main component (base) and which include:

- buffer solution;

- indicators - aqueous solution;

- surface-active substances (surfactants) - aqueous solution.

For granules, white perlite granulated with a fraction of 1 to 6 mm, from 96 to 99% in the composition of the finished product (indicator granules), is used as a base. Mass fraction of moisture in perlite is from 2 to 5%.

The buffer solution provides neutral acidity of perlite granules with a concentration in the solution of 100 ml / mol with a total fraction of up to 5% by volume of the base.

The indicator aqueous solution (hereinafter - IRV) with a total fraction of up to 10% by volume of the base includes: an indicator of the pH level and a surfactant (hereinafter - surfactant).

As an indicator of the pH level of water-soluble, you can use:

- methyl red C.I, Acid Red 2, CAS: 63451-28-5

- Alizarin C.1.58000, CAS: 72-48-0

- bromocresol purple, CAS: 115-40-2

- bromotimol blue, CAS: 76-59-5

- phenol red, CAS: 143-74-8

- neutral red, CAS: 553-24-2.

The indicated components of the indicator can be used both separately and in combination of two or more of the listed indicators. The concentration of the pH indicator in the IRV is from 0.01 to 2.0%.

Water-soluble polymer - a surfactant (hereinafter - surfactant), capable of forming a water-soluble film. The concentration of water-soluble polymer in the IRV from 10 to 40%. As a surfactant, you can use:

- polyvinyl alcohol - CAS: 98002-48-3, formula - (CH ₂ CHOH) n

- polyvinyl methyl ether - CAS: 9003-09-2, formula - (CH ₂ CH (OCH ₃ )) n

- polyvinylpyrrolidone - CAS: 9003-39-8, formula - (C ₆ H ₉ ON) n

- polymethacrylamide - CAS: 25014-12-1, formula - (C ₄ H ₇ NO) n

- polyoxymethylene - CAS: 30525-89-4, formula - (CH ₂ O) n

- polyethylene oxide - CAS: 25322-68-3, formula - (C ₂ H ₆ O ₂ ) n

- polymethacrylic acid - CAS: 25087-26-7, formula - (C ₄ H ₆ O ₂ ) n.

Distilled water is also used - 55-85% of the volume of IRV.

An aqueous solution of a surfactant and an aqueous solution of the indicator is applied to the finished perlite granules by fine spraying through a nozzle. Treated granules are dried.

Previously, an aqueous buffer solution is applied to the base, the temperature of the solution is 20-25 ° C.

Perlite granules are dried at a temperature of +100 to + 200 ° C for 5 minutes.

An aqueous solution of the polymer (surfactant) and indicator is applied to the finished perlite granules (base) by fine spraying.

The intrinsic temperature of the aqueous solution when applied to the substrate is from +15 to + 45 ° C.

The treated granules are dried. The drying temperature of the granules is from +40 to + 200 ° C. The drying time of the granules is from 5 to 30 minutes. Mass fraction of moisture in perlite is from 2 to 5%. For drying granules, any industrial and laboratory equipment of the “drying cabinet (SH)” type can be used, which can provide a temperature inside the drying chamber in the range from +40 to + 200 ° С.

Indicator granules for express determination of a potential disease in cats during the interaction of urine with indicator granules are used as follows.

Indicator granules are poured on top of any toilet filler. Within 15-30 seconds after the urine enters the granules, a reaction occurs showing the degree of potential predisposition of cats to the disease or existing disease by changing the color of the granules. The results of the reaction are clearly visible for several hours, then the color gradually weakens due to evaporation.

Example 1

The urine acidity of a healthy cat is in the range of 6.0-6.5 units, while urine values can be in the range of 5 to 8 units.

Acidity above pH 6.5 contributes to the formation of struvite stones, the main cause of the urological syndrome of cats, including urolithiasis, or indicates an existing serious disease. The phenol red indicator changes its color from yellow to red in the pH range of 6.6-8.2. A change in the color of the granules from yellow to red after the urine enters the granules will indicate a higher level of urine acidity and, as a result, the animal's predisposition to the disease or its presence.

Example 2

The alizarin indicator changes its color from yellow to red when the acidity level changes from 5.5 to 7.1. The indicator bromocresol purple changes its color from yellow to violet with a change in acidity from 5.2 to 6.8. However, with the combined use of these indicators, a selective reaction to a change in acidity occurs. So, after urine enters the granules, a visible reaction occurs when the acidity changes in the intervals of 5.2-6.0 and 6.5-7.1.

The present invention is industrially applicable, can be manufactured using known substances and does not require special manufacturing techniques of the granules themselves. Express diagnostics allows you to quickly visually identify signs of the disease and prevent the onset of the disease by timely contact to the veterinarian. Moreover, this rapid diagnosis is carried out without visiting the clinic and conducting analyzes, as is customary in existing practice.

Examples of indicator granules with different ranges of urine acidity according to claim 1, containing the following components

Example 1 (All quantities are indicated in grams)

Water 200 Phenol red 0.2 Polyvinylpyrrolidone thirty Perlite 300

Example 2 (All quantities are indicated in grams)

Water 200 Methyl red 0.35 Polyvinyl methyl ether 35 Perlite 300

Example 3 (All quantities are indicated in grams)

Water 200 Bromotimol blue 0.15 Polyvinylpyrrolidone thirty Perlite 300

Example 4 (All quantities are indicated in grams)

Water 200 Alizarin 0.3 Polyvinyl methyl ether fifty Perlite 300

Example 5 (a mixture of indicators, all quantities are indicated in grams)

Water 200 Methyl red 0.15 Bromotimol blue 0.25 Polyvinylpyrrolidone 35 Perlite 300

Example 6 (a mixture of indicators)

Water 200 Alizarin 0.2 Bromotimol blue 0.15 Polymethacrylamide 60 Perlite 300

Example 7 (a mixture of indicators, all quantities are indicated in grams)

Water 200 Alizarin 0.30 Bromocresol purple 0.2 Polyvinyl alcohol fifty Perlite 300

The results of the use of the claimed funds according to claim 1 and their predisposition to cats to the following diseases:

Options for sets of indicator granules with different ranges for evaluating the acidity of urine according to claim 2, containing the following components

Example 8 (a set of indicator granules, all quantities are indicated in grams)

granules 1:

Water 200 Methyl red 0.35 Polyvinyl methyl ether 25 Perlite 300

granules 2:

Water 200 Phenol red 0.2 Polyvinylpyrrolidone twenty Perlite 300

Example 9 (a set of indicator granules, all quantities are indicated in grams)

granules 1:

Water 200 Methyl red 0.35 Polyvinyl methyl ether 25 Perlite 300

granules 2:

Water 200 Bromotimol blue 0.15 Polyvinylpyrrolidone thirty Perlite 300

Example 10 (a set of indicator granules, all quantities are indicated in grams)

granules 1:

Water 200 Alizarin 0.25 Bromocresol purple 0.25 Polyvinyl alcohol fifty Perlite 300

granules 2:

Water 200 Phenol red 0.2 Polyvinylpyrrolidone thirty Perlite 300

Specific examples (1-10) of the manufacture of indicator granules according to claim 1 and claim 2 are presented above.

Claims (3)

1. A tool for indicating the potential predisposition of cats to the disease, which is indicator granules, which are used as a basis perlite white, granular fraction of 1 to 6 mm with a content of 96 to 99% in the composition of the finished product, and as an indicator in the form of a solution water use a pH indicator and a surfactant, while methyl red, or alizarin, or bromocresol purple, or bromotimol blue, or phenol red, or mixtures thereof, are used as an indicator of pH level; quality of surface-active agent is used polyvinyl alcohol or polyvinyl ether, or polyvinyl pyrrolidone, or polymethacrylamide, or Polyoxymethylene, or polyethylene oxide, or polymethacrylic acid. 2. A tool for indicating the potential predisposition of cats to the disease, which is a set of indicator granules, which are used as a basis perlite white, granular fraction from 1 to 6 mm with a content of 96 to 99% in the composition of the finished product, and as an indicator in the form aqueous solution using a pH indicator and a surfactant, while for part of the indicator granules, methyl red, or alizarin, or bromocresol purple, or mixtures thereof, are used as an indicator of pH th part - bromotimol blue or phenol red, or mixtures thereof, as well as a surfactant is used polyvinyl alcohol or polyvinyl ether, or polyvinyl pyrrolidone, or polymethacrylamide, or Polyoxymethylene, or polyethylene oxide, or polymethacrylic acid. 3. A method for determining the health problems of cats, which consists in adding indicator granules that change color at pH greater than 6.5 to the cat litter, and recording color changes of said indicator granules when feline urine enters them, characterized in that in the cat litter indicator granules are additionally added to the toilet, which, when feline urine enters them, change color at a pH of more than 5.0 and less than 6.0 due to the inclusion of methyl red, or alizarin, or bromocresol purple, or cm This and indicator granules, which, when feline urine gets on them, change color at a pH of more than 6.5 and less than 9.2 due to the inclusion of bromotimol blue, or phenol red, or mixtures thereof, and the violation of the health of cats is judged by a change colors of indicator granules relative to their original color.