JP2006234475A - Sprayer filled with coloring type spraying reagent for health check, and health check method using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an instrument capable of detecting easily a specified component contained in the excretory substance of a human being or an animal, and to provide a health check method for the human being or the animal that uses the same. <P>SOLUTION: The present invention provides a sprayer filled with a coloring-type spraying reagent for health check capable of detecting the specified component contained in then fecal matter of the human being or the animal, and the human being or the animal is health-checked (1), by spraying directly the coloring-type spraying reagent for the health check, or (2) by bonding a label-like material, prepared by spray the coloring-type spray reagent for the health check, onto toilet paper, a pet sheet, toilet sands, an fecal matter portion, the surface of a toilet container for the human being or the animal, the inside of the toilet container for the human being or the animal, or the diaper for the human being or the animal, using the sprayer. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a sprayer filled with a color-developing spray reagent for health check and a health check method using the same. More specifically, the present invention relates to a nebulizer filled with a color check-type spraying reagent for health check for examining human or animal excrement and a health check method using the same.

  In today's aging society, home health management is extremely important. In particular, there is an increasing demand for simple urine sugar meters and urine test strips that can be easily and non-invasively examined. However, handling and maintenance of the urine sugar meter is particularly troublesome for elderly people. In addition, the examination using the urine test paper is accompanied by troublesome work such as urine collection and dirty hands during the examination.

  On the other hand, in the modern society where the nuclear family and the aging are advancing, the existence value of pets represented by dogs and cats at home is very high, and not only their own health status but also the health status of pets is extremely important for owners. It is a serious concern. Furthermore, in recent years, lifestyle-related diseases such as diabetes are not limited to human diseases, but there is a concern that the morbidity of pets may increase due to obesity.

  It is ideal if the health status of a pet that cannot be self-asserted by words can be managed by examining the urine and blood of the pet at each home. It was very difficult to do.

  For this reason, there are many cases where the health management of pets cannot be performed at home, and the detection of pet illnesses tends to be delayed, and medical examinations and treatments are often performed at animal hospitals and the like after the health condition has deteriorated. As a result, the treatment is performed after the medical condition is worsened, and in some cases, it is too late. Furthermore, although medical insurance related to pets has become widespread in recent years, the burden of medical expenses has become considerably large. For these reasons, there is a growing demand for pet owners to easily manage their health conditions at home, leading to early detection of pet illnesses.

In response to the above demands, a color reagent that changes color tone by reacting with a specific component contained in animal urine, such as so-called toilet litter for pets, can be used as a method for easily performing health management using urine in general households. A method has been proposed in which a urine absorbent is impregnated, and the health of the urine is detected by detecting the components of the urine by using the color of the reagent when the pet urinates.
Japanese Patent No. 1,512,480 Japanese Patent No. 2,727,474 Japanese Patent Laid-Open No. 04-114066 Japanese Patent No. 2,896,021 JP-A-10-229767 Japanese Patent Laid-Open No. 10-257832 JP-A-11-295306 Japanese Patent No. 3,352,425 JP 2003-279565 A

  However, although these methods are easy to operate, they are mainly related to toilet sand and toilet seats used for indoor pets and are effective for indoor pets, but are kept outdoors. There is a problem that it is difficult to use for pets. In addition, it is necessary to use specific toilet sand, toilet seats, and diapers, which may not be convenient for owners and pets.

  An object of the present invention is to provide a device that can easily detect a specific component contained in human or animal excrement and a method for checking the health of a human or animal using the same.

  An object of the present invention is to provide a sprayer filled with a color-type spraying reagent for health check that detects specific components contained in human or animal excrement, and using this, toilet paper, pet sheet, toilet sand, (1) Directly spraying a coloring spray reagent for health check or (2) Health on the excretion area, the surface of a human or animal toilet container, the human or animal toilet container, or a human or animal diaper This is achieved by performing a health check on a human or animal by applying a seal-like material prepared by spraying a color developing type spraying reagent for checking.

  The coloring sprayer for human or animal health check according to the present invention can easily detect a specific component contained in human or animal excrement by a method of spraying a reagent filled in the sprayer. Therefore, it can be used conveniently not only indoors but also outdoors, and can be used effectively not only for humans but also for health management of pets raised outdoors.

  As specific components contained in human or animal excrement, components that can be checked for health are sufficient, and components reflected in urine as a result of illness such as hydrogen ions, nitrite, glucose, ascorbic acid , Proteins, urea, uric acid, urea nitrogen, creatinine, blood (occult blood), ketone bodies, bilirubin, urobilinogen, bile acids and the like.

  The color-developing spray reagent for health check is a reagent for detecting the specific component contained in human and animal excrement by a change in color tone, and (1) In addition to the color former that reacts with the specific component and develops color Any reagent capable of detecting a specific component in urine is not particularly limited, and (2) reacts with an enzyme that generates a distinguishable substance that reacts with a color former to generate a color by reacting with the specific component and a distinguishable substance. Two or more types of color formers that develop color by color, or (3) an antibody that recognizes a specific component labeled with a distinguishable substance that reacts with the color former and a colorant that reacts with the distinguishable substance and develops color A mixture of reagents is used.

  In the case where the hydrogen ion concentration is detected and the acidity or alkalinity of urine is examined, those having a pH indicator as a main component can be mentioned. Examples of pH indicators include thymol blue, phenolphthalein, cresol red, phenol red, neutral red, bromothymol blue, bromocresol purple, bromophenol red, p-nitrophenol, methyl red, bromocresol green, tetrabromophenol Examples include indicators such as blue, chlorophenol red, methyl orange, ethyl orange, bromophenol blue, brilliant yellow, and congo red bromocresol blue. These indicators can be used individually by 1 type or in combination of 2 or more types. Examples of using the above indicators in combination include a mixed indicator of methyl orange and bromocresol green, a mixed indicator of methyl red and bromothymol blue, a mixed indicator of bromocresol purple and bromothymol blue, and the like.

  In the case of detecting glucose, for example, glucose oxidase and peroxidase enzymes include ortho-dianisidine, ortho-tolidine, and 2,2′-azino-di- (3-ethylbenzthiolysone), which are reduced dyes. -6-sulfonate [ABTS], 2,7-diaminofluorene dihydrochloride, N- (3-sulfopropyl) -3,3 ', 5,5'-tetramethylbenzidine sodium Used. From glucose, gluconic acid and hydrogen peroxide are produced by the action of glucose oxidase, and further, a reduced dye reacts by the action of peroxidase to produce an oxidized dye. A check can be made.

  In the case of detecting ascorbic acid, for example, those containing Tillmans reagent as the main component can be mentioned. Reduced pigments are produced by the reaction of oxidized Tillmans reagents such as 2,6-dichlorophenolindophenol with reduced ascorbic acid, and a health check is performed by colorimetric or semi-quantitative determination of this. Can do. It is also possible to predict false negatives due to the presence of ascorbic acid in other test items such as urine sugar, occult blood, bilirubin or nitrite.

  In the case of detecting nitrite, for example, one containing a grease reagent as a main component can be mentioned. Usually, nitrates are present in urine, but when a large amount of bacteria are present in urine, nitrates are reduced to nitrites by the action of enzymes contained in the bacteria. By reacting the nitrite with a grease reagent such as nitrite arsanilic acid or sulfamine, a pink azo dye is formed. By colorimetrically or semi-quantitatively determining this, it is possible to determine whether urine contains bacteria such as Escherichia coli, Shigella, Salmonella and Staphylococcus.

  In the case of detecting a protein, an indicator that identifies the presence or absence of a protein by a protein error method is used as a main component. Examples of the indicator include tetrabromoblue, tetrabromothymol blue, tetra Examples include bromophenol blue. Tetrabromoblue forms a complex when protein is present in the specimen, and the color tone changes from yellow, which is an acidic color, to blue, which is a basic color.

  Further, as another method for detecting a protein, a method using an antibody can be mentioned, and an example thereof includes a pregnancy test drug. Human chorionic gonadotropin (hCG) is found in urine during pregnancy, and blue latex-conjugated anti-hCG antibody is used for this detection. The blue latex-bound anti-hCG antibody aggregates due to an antigen-antibody reaction when hCG is present in urine and turns blue.

  In the case of quantifying or semi-quantifying urea, for example, a pH indicator capable of measuring between pH 5 and 9 and a mixture of urease and pH indicator can be mentioned. In other words, after measuring the pH of urine using only a pH indicator, a mixture of a pH indicator and urease is allowed to act on urine, so that ammonia, which is an identifiable substance produced by the reaction of urease and urea, Since it is generated in proportion to the amount, the value is higher than the pH of urine alone, and the more urea, the higher the pH. The amount of ammonia produced, that is, the amount of urea can be known.

  In the case of detecting uric acid, for example, the enzyme group of uricase and peroxidase is reduced to ortho-dianisidine, ortho-tolidine, ABTS, 2,7-diaminofluorene dihydrochloride, N- (3- A combination of sulfopropyl) -3,3 ′, 5,5′-tetramethylbenzidine sodium and the like is used. From urea, hydrogen peroxide is produced by the action of uricase, and the reduced dye reacts by the action of peroxidase to produce oxidized dye, which is then colorimetrically or semi-quantitatively checked for health. be able to.

  In the case of detecting urea nitrogen, for example, a combination of urease and phenol is used. Urea nitrogen produces ammonia by the action of urease, and a health check is performed by colorimetric or semi-quantitative determination of indophenol blue produced by reaction of this with phenols such as ortho-cresol and bromcresol green. be able to.

  In the case of detecting creatinine, for example, creatininase, creatinase, sarcosine oxidase, peroxidase enzyme group ortho-dianisidine, ortho-tolidine, ABTS, 2,7-diaminofluorene dihydrochloride which is a reduced type dye A combination of a salt, sodium N- (3-sulfopropyl) -3,3 ′, 5,5′-tetramethylbenzidine and the like is used. From creatinine, creatine is produced by the action of creatininase. Creatinase acts on this to form sarcosine, and sarcosine oxidase further produces glycine and hydrogen peroxide. Furthermore, the presence of a renal disease can be confirmed mainly by colorimetric or semi-quantification of the oxidized dye by the reaction of the reduced dye by the action of peroxidase.

  In the case of detecting occult blood, those containing a reduced dye, an organic peroxide or the like as a main component can be mentioned. Examples of reduced dyes include ortho-tolidine and benzidine, and examples of organic peroxides include cumene hydroperoxide. When occult blood is present in the urine, the occult blood combines with the cumene hydroperoxide described above to generate nascent oxygen, and this oxygen immediately reacts with ortho-tolidine, benzidine, etc. to cause the coloring of this pigment.

  In the case of detecting a ketone body, for example, a combination of nickel chloride and sodium nitroprus pseudo is used. In this combination, the hue changes from pale yellow to reddish purple in the presence of acetoacetic acid in the specimen.

  In the case of detecting bilirubin, for example, one containing a diazonium salt as a main component can be mentioned. Azo produced by reacting diazonium salts such as bis-2,4-dichlorobenzenediazonium chloride, 2,4-dichlorobenzenediazonium tetrafluoroborate, p-benzenediazoniumsulfonic acid with bilirubin under acidic conditions The presence or absence of hepatobiliary disease can be confirmed mainly by colorimetric or semi-quantitative determination of the compound.

  In the case of detecting urobilinogen, for example, one containing a diazonium salt as a main component can be mentioned. Diazonium salts such as 4-methoxybenzenediazonium tetrafluoroborate, 3,4-methylenedioxybenzenediazonium tetrafluoroborate, 3,3'-dimethoxy-4,4'-diazobiphenyltetrafluoroborate The presence or absence of hepatobiliary disease can be confirmed mainly by colorimetric or semi-quantitative determination of the azo compound produced by reacting with urobilinogen under acidic conditions.

  These color-developing spray reagents for health check are filled in a sprayer in liquid, solid or powder form, preferably solid or powder form. Liquid reagents can be nebulized as they are, but solid or powdered ones can be dissolved in a solvent that can dissolve these reagents, such as water, alcohol, and a buffering solution immediately before use. Used. By using a solid or powdery reagent dissolved immediately before use, the stability of a liquid unstable reagent can be improved. In any case, alcohols such as ethanol are preferably added and used for the purpose of improving the drying rate after spraying the reagent.

  In addition to these reagents, at least one of a desiccant, a deodorant, an antibacterial agent, a preservative, a stabilizer, and a preservative is further added as necessary.

  The desiccant is used to prevent alteration of the reagent due to moisture absorption when the reagent filled in the sprayer is solid or powdery, and includes silica gel, quicklime, calcium chloride, silica alumina gel, alumina, sulfuric acid, Examples include phosphorus oxide, sodium hydroxide, barium oxide, potassium carbonate, dehydrated copper sulfate, and sodium metal.

  Examples of the deodorizer include silica gel, zeolite, activated carbon, fullerene, ceramics and the like. Antibacterial agents include methylene blue, titanium oxide, titanium phosphate, catechin and the like. Examples of the preservative, stabilizer, or preservative include sorbic acids, celluloses, thickening polysaccharides, thickening stabilizers, alginic acids, benzoic acids, phenols, and the like.

  Each of the above reagents is used by being filled in a sprayer. Each reagent is filled in the same container, or filled in separate containers and mixed at the time of spraying, or used by a procedure such as spraying a solution containing a color former after spraying some reagents. The When each reagent is filled in a separate container and mixed at the time of spraying, if the color former and enzyme are mixed for a long time, a part of the reaction will proceed, resulting in a decrease in detection sensitivity. It is possible to prevent problems such as In addition, when a solution containing a color former is sprayed after spraying a part of the reagent, the colorant becomes vivid by using the color former after sufficiently generating a discriminating substance, There is an effect of preventing color fading over time of coloring.

  The sprayer is not particularly limited as long as it can spray the reagent, and examples thereof include those using air pressure such as a spray or those filled with gas. The container material is not particularly limited, but aluminum, plastic Steel, glass, paper, etc. are used. Furthermore, a light-shielding container considering the stability of each reagent is preferable.

  The sprayer body is preferably displayed with a color sample showing the color change of the color former that has reacted with the specific component in the excrement using the displayed color sample. By using this, it becomes possible to determine the content of the specific component or the presence or absence of the content.

  Human or animal health check methods include toilet paper, pet sheets, toilet sand, excretion points, human or animal toilet container surfaces, human or animal toilet containers, human or animal diapers (1 ) Directly spraying the health-checking color-developing reagent or (2) sticking a seal-like material created by spraying the health-checking coloring reagent with a sprayer. Further, the spraying or sticking of the seal-like material is performed at a urination location after urination or at a urination location in advance.

  Next, the present invention will be described with reference to examples.

Example 1
A solution of 58 units of glucose oxidase (Sigma), 44 units of peroxidase (Wako Pure Chemicals) and 2 mg of ortho-tolidine (Wako Pure Chemicals) in 20 ml of ultrapure water was placed in a nebulizer, and 500 mg / dl was added. When a solution in which glucose was dissolved in a phosphate buffer solution (pH 6.0) at a concentration was sprayed onto a test object soaked in tissue paper, it turned blue after about 10 seconds. Thus, it was shown that glucose (urine sugar) in urine can be detected.

Example 2
In Example 1, a tissue paper soaked with a solution in which glucose was dissolved in a phosphate buffer solution (pH 6.0) at a concentration of 500 mg / dl as a test object was further dried at room temperature for 1 hour. When used, it turned blue about 10 seconds after spraying. As a result, it was shown that glucose (urine sugar) in urine can be detected even in urine that is in a dry state, for example, at the urination site of a pet toilet.

Comparative Example In Example 1, when a tissue buffer soaked with a phosphate buffer solution (pH 6.0) not containing glucose was used as a test object, no coloration was confirmed. Thus, it was shown that the blue coloration shown in each example was due to the detection of glucose.

Claims (8)

  A sprayer filled with a color-developing spray reagent for health check that detects specific components contained in human or animal excrement.   The color-specific spray for health check according to claim 1, wherein the specific component is hydrogen ion, nitrite, glucose, ascorbic acid, protein, urea, uric acid, urea nitrogen, creatinine, blood (occult blood), ketone body, bilirubin or urobilinogen. Nebulizer filled with reagents for use.   The sprayer filled with the coloring reagent for health check according to claim 1 or 2 further added with at least one of desiccant, deodorant, antibacterial agent, preservative, stabilizer and preservative.   4. A sprayer filled with the color-developing reagent for health check according to claim 1, 2 or 3, which is liquid, solid or powder.   A sprayer filled with the color-developing reagent for health check according to claim 4, which is used by being dissolved in alcohols.   6. A sprayer filled with a health check coloring reagent according to any one of claims 1 to 5, wherein a color sample of the color former is displayed on the sprayer body.   With the sprayer according to any one of claims 1 to 6, toilet paper, pet sheet, toilet sand, excretion site, human or animal toilet container surface, human or animal toilet container, human or animal diaper , (1) Directly spraying a coloring reagent for health check, or (2) Applying a seal-like material created by spraying a coloring reagent for health check How to check the health of a person or animal. The method for checking human or animal health according to claim 7, wherein spraying or sticking of a seal-like material is performed before or after excretion.