RU2356558C2 - Method of producing antioxidant enterosorbent - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for peroxidate excretion gastrointestinal tract. There is disclosed method of producing antioxidant enterosorbent involving that natural aluminium silicate is powdered, mixed with water in ratio 1:10, actively mixed. 2/3 volume of liquid is poured out to the other tank 20 sec later where it is settled for 25 min. Then the liquid is rejected, while the residual deposit representing sorbent SB-1 is dried up in air flow at 20-35°C and 100-105°C during 6 hours. Washed potatoes are milled, mixed with water in ratio 1:5, kept in constant stirring for 5 hours at 10-20°C, settled for 5 hours at 10°C. The liquid is decanted and filtered. The sorbent SB-1 is added to the filtrate in ratio 5:1. The mixture is constantly stirred during 5 hours at 5-10°C, settled during 1 hour. The liquid is poured out, and deposition is washed in water in ratio 1:10, mixed for 2 hours at 5-10°C, settled for 5 hours at the same temperature. The liquid is poured out, while deposit containing the end product is dried in air flow. Said enterosorbent contains high-porous natural aluminium silicate - silica clay of the Astrakhan area characterised with extended surface, and antioxidants.

EFFECT: invention ensures production of new high-performance enterosorbent antioxidative to peroxidates.

1 ex, 2 tbl

Description

The invention relates to medicine and can be used to remove peroxide compounds from the gastrointestinal tract.

Issues of oxygen metabolism in the human body are the objects of constant attention of physicians, chemists and biochemists. Especially now, when it became apparent that the excessive content of oxygen and its active radicals cause radical chain oxidation processes in the cells of the body, leading to significant disturbances in their normal metabolism.

Pharmacological correction of oxidative stress is carried out using biologically active substances, in particular antioxidants.

A known method of obtaining a natural antioxidant (AO) [1]. The method is as follows: the unmilled sunflower husk obtained after the seeds were crushed in the technological cycle for the production of sunflower oil is mixed with water in a mass ratio of water: husk = 10: 1, brought to a boil and boiled for 30-55 minutes, and then filtered. The solvent (water) is evaporated by boiling, bringing the extract to a jelly-like state.

The specified method has the disadvantage that the obtained AO simultaneously has the property to destroy blood, is a hemolytic [1]. The presence of hemolytic activity not only reduces the beneficial properties of AO, but can lead to damage to health.

There is also a method of producing carbon enterosorbent [2], used in acute poisoning and other endo- and exogenous intoxications, including those arising from radiation exposure. This method is based on grinding activated carbon fibers, moistening them, treating with a binder, pressing and drying. Grinding is carried out to a monofilament length of 1-3 mm and 30-100 microns, and starch gel is used as a binder, which is introduced into the fibers to a final concentration of 2-6%.

The disadvantage of this development is the use of carbon fiber particles having a length at the level of millimeters, such particles have a sharply reduced sorption capacity compared to particles having nanoscale sizes. The effectiveness of carbon as a sorbent almost completely disappears if these carbon particles are coated with starch. When it enters the gastrointestinal tract, starch dissolves, while most of the components are desorbed from starch.

Closest to the described is a method for producing a natural melanoid antioxidant [3]. This method is as follows. Unmilled sunflower husk is washed with water, the washed husk is dried at a temperature of 90-110 ° C to a friable state; mix water with husk in a mass ratio of water: husk = 10: 1, bring to a boil and boil for 30-55 minutes, and then filtered. The filtrate is a liquid antioxidant. A food adsorbent (silica gel, zeolite, etc.) is added to the obtained AO and evaporated to a jelly-like state.

The disadvantage of this melanoid AO is that it has a narrowly targeted effect, mainly preventing the oxidation of fatty acids. Meanwhile, enterosorbent with antioxidant functions should deactivate significant amounts of various peroxide compounds.

The aim of this invention is the creation of a new highly effective enterosorbent that carries antioxidant functions in relation to peroxides.

As the basis of the proposed enterosorbent, a natural aluminosilicate (flask) is used, having the following composition: SiO ₂ 75-80%, Al ₂ O ₃ 22-18%, Fe ₂ O ₃ 0.5-1%, H ₂ O 0.2- 0.5%, CaSO ₄ 0.3-0.5%, CaCO ₃ 0.12-0.8%. Flask has a high sorption capacity in relation to a large group of organic and inorganic compounds. Sorbents from flasks of varying degrees of dispersion, not subject to any chemical treatment, simply milled and sieved by particle size, were called SV-1 (50-300 nm), SV-2 (0.01-0.03 mm), SV-4 (2-5 mm), SV-10 (8-12 mm), where the numbers in parentheses are the approximate particle diameters. To obtain enterosorbent with antioxidant properties, the sorbent SV-1 is used. To improve the sorption ability with respect to peroxide compounds, antioxidants, such as peroxidase, catalase, and ascorbic acid, obtained from aqueous extracts of plant material, are adsorbed onto the sorbent. As materials of plant origin, potatoes, turnips, peanuts, horseradish roots, etc. are used.

The following example is presented in order to more fully illustrate the invention.

Example 1. The first stage of obtaining enterosorbent. The natural aluminosilicate is ground to a powder state, mixed with water in a ratio of 1:10, mixed vigorously, after 20 seconds 2/3 of the liquid volume is poured into another vessel, sedimented for 25 minutes, then the liquid is discarded, and the remaining precipitate, which is a sorbent SV-1 , dried in a stream of air at 20-35 ° C and 6 hours at 100-105 ° C.

The second stage of obtaining enterosorbent. The washed potatoes are crushed, mixed with water in a ratio of 1: 5, maintained with constant stirring for 5 hours at 10-20 ° C, stand for 5 hours at 10 ° C, the liquid is decanted, filtered, the sorbent SV-1 in a ratio of 5 is added to the filtrate: 1, the mixture is constantly stirred for 5 hours at 5-10 ° C, left to stand for 1 hour, the liquid is drained, the precipitate is washed with water at a ratio of 1:10, stirred for 2 hours at 5-10 ° C, left to stand for 5 hours at the same temperature, the liquid is drained and the precipitate containing the target product is dried in a stream of air.

Ready enterosorbent SV-1-AO is a gray powder, odorless and tasteless. It does not dissolve in water and biological fluids.

The antioxidant properties of the enterosorbent SV-1-AO were studied by the reaction of the interaction of peroxidase, catalase, or vitamin C located on its surface with hydrogen peroxide. In this case, various reactions proceed with the formation of water or oxygen.

The amount of hydrogen peroxide is judged by the amount of sodium thiosulfate used for titration of elemental iodine, which is formed quantitatively during the oxidation of hydroiodic acid (KJ + H ₂ SO ₄ ) with hydrogen peroxide. The results are presented in tables 1 and 2.

Table 1 Sorbent test results to establish antioxidant properties The volume of hydrogen peroxide, cm ³ The amount consumed is 0.01 n. sodium thiosulfate solution, cm ³ Without sorbent Sorbent SV-1-AO Sorbent SV-1 0 0 0 0 0.1 0.5 0.1 0.6 0.2 0.98 0.36 0.92 0.4 1.9 0.3 1,6 0.8 3.8 0.6 3.4 one 4.7 1,1 4.4 5 23,2 5,4 20.5 10 44.5 10,2 42,2

table 2 The study of the decomposition of hydrogen peroxide on SV-1-AO Time s The volume of sodium thiosulfate, went to the titration, cm ³ T = 278K T = 298K 0
5
10
120
240
360
600 38,0
36.8
35.9
5.2
3.4
2.1
0.34 38,0
31.5
30.9
4.8
2.2
1.3
0.2

The results given in Tables 1 and 2 indicate that the antioxidant properties of the enterosorbent CB-1-AO when using different concentrations of hydrogen peroxide are well manifested. The decomposition rate of H ₂ O ₂ at different temperatures is quite high. For at least 25-30 s, the concentration of H ₂ O ₂ in the studied system decreases by 100 times.

The possibility of deactivation of hydrogen peroxide in the gastrointestinal tract of animals with the use of enterosorbent SV-1-AO (experience) and with the use of SV-1 (i.e., a sorbent that does not have antioxidant properties) was clarified on rats (10 albino males). with a mass of 130-135 g each).

The rats were fed oats, which were kept in a solution of hydrogen peroxide (in this case, partial adsorption of H ₂ O _{2 by} oat grains took place). After 10 hours, the content of hydrogen peroxide in grains is reduced by about half, and after 2 hours after processing, by 10%. After the feed was soaked in an aqueous solution of H ₂ O ₂ , a sample of oats was soaked in water, then the content of hydrogen peroxide in the feed (mg / g) was determined by iodometric titration with sodium thiosulfate. Separately prepared gruel SV-1-AO (5 g per 20 cm ^{3 of} water) and 100 g of oats was added to this gruel. The mass was constantly mixed, then spread on glass and dried using a fan.

Initially, all animals were fed oat-treated with hydrogen peroxide (half of the prescribed amount according to the diet). After 1 h, the experimental group (5 pcs.) Was given oats treated with the enterosorbent SV-1-AO, and the control group (5 pcs.) Was given untreated oats. Within 24 hours, the reaction of animals of both groups was observed. The experimental group for 3 hours, as well as the control, in contrast to animals that were not given food with H ₂ O ₂ , was lethargic, with some inhibition in movements. Further, the behavior of the experimental animals became quite normal, and the control remained sluggish, after 13 hours 2 rats died, after 23 hours - another one. On the next day, the behavior of the experimental and control animals became identical, the death of animals was not observed.

Thus, experiments on rats led to the conclusion that the new enterosorbent SV-1-AO has pronounced antioxidant properties.

Information sources

1. Kashevatskaya L.A., Alekseeva Yu.K., Matygina L.M., Minasyan N.M., Proskurina V.L., Tsygankova G.E. / A method of obtaining natural antioxidants: A.S. 502012 USSR, MKI C11B 5/00; declared 1973.10.10; publ. February 2, 1976.

2. Yeretskaya EV, Pimonenko N.Yu., Nikolaev VG, Pimonenko Yu.N. / A method of producing a carbon enterosorbent: RF Patent No. 2027437, IPC A61K 33/44; declared 04/04/16; publ. 1995.01.27.

3. Zhorina L.A., Kashevatskaya R.N., Ivanov A.L., Ivanov V.L. / A method of obtaining a natural melanoid antioxidant: RF Patent No. 2281779, IPC A61K 36/28; declared 2004.11.26; publ. 2006.08.20.

Claims (1)

A method of producing an enterosorbent with antioxidant properties to remove peroxide compounds from the gastrointestinal tract, characterized in that the natural aluminosilicate is ground to a powder state, mixed with water in a ratio of 1:10, mixed vigorously, and 2/3 of the liquid volume is poured into another in 20 seconds the vessel is left to stand for 25 minutes, then the liquid is discarded, and the remaining precipitate, which is a sorbent SV-1, is dried in a stream of air at 20-35 ° C and 6 hours at 100-105 ° C; washed potatoes are crushed, mixed with water in a ratio of 1: 5, kept under constant stirring for 5 hours at 10-20 ° C, stand for 5 hours at 10 ° C, the liquid is decanted, filtered, the sorbent CB-1 in a ratio of 5 is added to the filtrate: 1, the mixture is constantly stirred for 5 hours at 5-10 ° C, left to stand for 1 hour, the liquid is drained, the precipitate is washed with water at a ratio of 1:10, stirred for 2 hours at 5-10 ° C, left to stand for 5 hours at the same temperature, the liquid is drained and the precipitate containing the target product is dried in a stream of air.