RU2467760C1 - Method of obtaining modified enterosorbent - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to method of obtaining enterosorbent, which includes application of plant extract on prepared highly disperse basis of silicon dioxide by mixing plant extract in ratio 3:1 with basis during 10-30 minutes at room temperature, decantation after complete sedimentation and drying at temperature 60°C until dry homogeneous powder is obtained. As plant extract used is aloe juice, obtained from washed, dried and crushed aloe leaves, preliminarily kept in the dark at temperature 4-8°C for 10-12 days. Basis is prepared by washing with distilled water and drying at room temperature.

EFFECT: invention ensures obtaining enterosorbent with sorption and antioxidant properties in few steps.

1 tbl, 4 dwg

Description

The invention relates to the field of biotechnology, specifically to a method for producing an enterosorbent having antioxidant activity, and can be used in medicine, veterinary medicine and animal husbandry.

Enterosorbents are products used to bind metabolites, toxins and other substances in the digestive tract. They are promising in solving the problems of regulating the nutrition of humans and animals, to reduce the intake of environmentally harmful substances (including radionuclides, pesticides, heavy metals), and the prevention and treatment of a number of diseases.

As sorbents, zeolites, bentonites, activated carbon, microcrystalline cellulose, lignins and their derivatives are widely used. One of the contraindications for the widespread use of these substances is their indiscriminate adsorption, which leads to the removal of beneficial substances (essential amino acids, vitamins, monosaccharides, etc.) from the body. A promising area of biotechnology is the production of modified sorbents with desired or predicted properties. For these purposes, both different matrices and various modifying agents are used.

A known method of producing enterosorbent based on lignin (Patent RU No. 2302866. "Enterosorbent and method for its production", published. 07.20.2007). Lignin is modified by applying iodine. The disadvantage of this method is that the resulting bactericidal preparation with prolonged use can have an irritating effect on the gastric mucosa.

In patent RU No. 2257951 "Method for producing enterosorbent" (published. 08/10/2005), enterosorbent based on casein-modified silica is described. The disadvantage of this method is the lack of antioxidant activity in the resulting product.

In patent RU No. 2197145 "Modified dietary fiber" (published. 01.27.2003) enterosorbent based on dietary fiber with the addition of selenium is described. The disadvantage of this method is that selenium in the form of inorganic compounds - selenites - is mechanically added to a mixture of dietary fiber. The biological activity of enterosorbent is not described.

In the patent RU No. 2180230 "Enterosorbent" (publ. 10.03.2003) describes a method for producing enterosorbent, which includes cellulose, pectin, dry rosehip extract. The disadvantage of this method is that the enterosorbent is a mechanical mixture of dry components. Patent RU No. 2180577 "Enterosorbent" (publ. March 20, 2003) describes a method for producing a sorbent based on microcrystalline cellulose to improve the therapeutic and prophylactic properties and taste of a product containing dry rosehip extract and sorbitol, to which an additional liquid extract of Rhodiola rosea or ascorbic acid and a sugar substitute. The method is carried out by mechanical mixing of the components and subsequent tableting.

The closest in its features adopted for the prototype is the patent RU No. 2356558 "Method for the production of enterosorbent with antioxidant properties", which consists in preparing at the first stage a finely divided base by grinding to powder state, mixing with water and intensively mixing natural aluminosilicate, which is then dried first in a stream of air at 20-35 ° C and then for 6 hours at 100-105 ° C; and at the second stage, a plant extract from potatoes is prepared by chopping it, mixing it with water in a ratio of 1: 5, holding it with constant stirring for 5 hours at 10-20 ° С, settling for 5 hours at 10 ° С, decanting and filtering, after which the sorbent SV-1 was added to the filtrate in a ratio of 5: 1, the mixture was constantly stirred for 5 hours at 5-10 ° C, sedimented for 1 hour, the liquid was drained, the precipitate was washed with water in a ratio of 1:10, stirred for 2 hours at 5-10 ° C, they stand for 5 hours at the same temperature, the liquid is drained, and the precipitate containing the target product is dried in a stream of air.

The disadvantage of this method is that it is multi-stage and requires using lower temperatures during mixing and settling, and the resulting product has antioxidant properties only with respect to peroxides.

The objective of the invention is to provide a method for producing an effective enterosorbent, characterized by a lower complexity and a wider range of antioxidant properties of the finished product.

The problem is solved by the proposed method, in which highly dispersed silicon dioxide is used as the basis of the sorption material, and native aloe juice, which is a modifying agent, is used as a plant extract.

Technical result:

- reducing the number of stages of the method;

- the resulting composite not only preserves the sorption and antioxidant properties of the components, but also surpasses them in the listed indicators;

- a wide spectrum of enterosorbent action, due to the anthracene derivatives contained in aloe juice, which have antioxidant, wound healing and immunomodulating properties.

The proposed method for producing a modified enterosorbent (MES), including applying to the prepared highly dispersed base plant extract, differs from the prototype by the signs:

- as a highly dispersed base, a substrate of highly dispersed silicon dioxide (VDK) is used;

- as a plant extract using aloe juice as a modifying agent;

- the method of preparation, namely: VDK is mixed with the obtained aloe juice in a ratio of 3: 1 for 10-30 minutes at room temperature, decanted after complete settling and dried in an oven at 60 ° C until an air-dry state is obtained, which allows to preserve the biological activity of the anthracene derivatives that make up aloe juice.

No technical solutions have been found from the prior art in which to obtain a modified sorbent, a method is used by mixing finely divided silica with aloe juice, which suggests that the claimed method meets the criterion of "novelty." The criterion of "inventive step" confirms that the enterosorbent obtained by the proposed method not only preserves the sorption and antioxidant properties of the components, but also surpasses them in the listed indicators.

The method is characterized by the following graphic materials:

Figure 1. IR spectra of the proposed modified enterosorbent (1) and VDK sorbent (2).

Figure 2. Nitrogen sorption isotherm at MES (TriStar II 3020 gas adsorption analyzer).

Figure 3. Nitrogen sorption isotherm on VDK (TriStar II 3020 gas adsorption analyzer).

Figure 4. Kinetic oxidation curves of unrefined sunflower oil, where • - idle; ■ - in the presence of 1 ml of aloe juice; ▲ - in the presence of 0.1 g of MES.

An example implementation of the method.

A substrate of finely divided silica (VDK) is preliminarily prepared by washing with distilled water and drying at room temperature. Then, aloe juice (Aloe arborescens Miller) containing anthracene derivatives is applied to the prepared substrate. To obtain juice, large, more developed aloe leaves are used, kept in the dark at a temperature of 4-8 ° C for 10-12 days to activate biogenic stimulants in the plant. Then they are washed, dried, crushed and squeezed. The resulting juice is mixed with VDK in a ratio of 3: 1 for 10-30 minutes at room temperature, decanted after complete settling, and dried in an oven at a temperature of 60 ° C until a dry, homogeneous powder is obtained.

In the proposed method, the retention of the modifying agent on the sorbent is ensured by physicochemical interactions, which is confirmed by IR spectroscopy. When comparing the spectrum (1) of the obtained enterosorbent and the spectrum (2) of the VDK sorbent used as the base, shown in Fig. 1, it is seen that highly dispersed silicon dioxide (2) does not have catalytic properties, and the appearance of a peak in the spectrum of enterosorbent (1) in the region of 1598 cm ^-1 (characteristic of aromatic rings) and an increase in the peak intensity in the region of 985, 803 cm ^-1 (vibrations of the CC bond) allow us to speak of the physicochemical interaction of the substrate and native juice, which guarantees the constancy of biologically active properties enterosorbent. The intense band in the region of 1580 cm ^-1 is due to the presence of aromatic rings in anthraquinone. The carbonyl groups of the quinoid ring give a band in the region of 1730 cm ^-1 . The band in the region of 3397 cm ^-1 indicates the existence of β-hydroxyl groups. Peaks in the region of 1373 and 1093 cm ^{–1 are} due to vibrations of the С – С and С – O groups, respectively.

The sorption activity of the VDK substrate and the obtained MES enterosorbent are shown in FIGS. 2 and 3. An important characteristic of the sorbent is the specific surface that determines its activity. The specific surface area of VDK and MES was estimated for nitrogen sorption. Calculated by the BET method (the method proposed by Brunauer, Emmett and Taylor), it was 156.4799 ± 3.0521 m ² / g and 91.7837 ± 1.0489 m ² / g, respectively. The difference in the specific surface area of the VDK and MES allows one to positively assess the role of MES as an enterosorbent, since more than 40% of the obtained sample is occupied by the phytocomponent (in particular, anthracene derivatives).

The antioxidant activity of the obtained composite is illustrated in figure 4. The oxidation dynamics of unrefined sunflower oil shows that the resulting enterosorbent has a much greater antioxidant activity compared to native aloe juice.

Relative antioxidant activity (AOA _rel ) was determined by the conductometric method.

Comparative results of the relative antioxidant activity of the proposed MES, starting components and ascorbic acid are presented in the table.

Values of relative antioxidant activity (AOA _rel )

sample AOA _Rel VDK 0.43 aloe juice 1,5 MES 2 vitamin C 1.94

The table shows that the antioxidant activity of the modified sorbent obtained by the proposed method is almost 5 times higher than the AOA _{relative to the} substrate, 33% higher than the AOA _{relative to} aloe juice, and 3.5% higher than the arithmetic sum of the values of AOA _{relative to the} substrate and AOA _{relative to} aloe juice and comparable to the AOA _relative ascorbic acid.

Thus, the task of creating a less labor-intensive method of obtaining an effective enterosorbent that combines sorption properties with a wide range of antioxidant properties has been solved.

The proposed method allows it to be used to obtain an effective enterosorbent with pronounced biological activity: the silicon base of MES, due to its high dispersion, allows it to be fixed on the cell surface, and in combination with the herbal medicinal component, create conditions for an effective antioxidant effect in the body.

Claims (1)

A method of producing an enterosorbent, comprising applying to a prepared fine base plant extract, characterized in that as a plant extract using aloe juice obtained from washed, dried and crushed aloe leaves, previously aged in the dark at a temperature of 4-8 ° C for 10- 12 days, which is mixed in a 3: 1 ratio with a base, which is used as a highly dispersed silicon dioxide, previously prepared by washing with distilled water and drying at room temperature; the juice with the base is stirred for 10-30 minutes at room temperature, decanted after complete settling, and dried at a temperature of 60 ° C until a dry, homogeneous powder is obtained.

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