RU2353930C2 - METHOD OF RAPID BLOOD SERUM ANALYSIS FOR b-LIPOPROTEIDS - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention aims at industrial screening and periodical health examination, low-equipment hospital application. The disclosed method allows for approximate concentration of b-lipoproteids based on visual estimation enabled by reading the developed text through the test-tube layer containing the analysed substance.

EFFECT: there is developed simplified method of b-lipoproteid content analysis, possibility to estimate their approximate concentration without instrumentation means for examination of considerable number of patients.

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Description

The invention relates to medicine, in particular to laboratory diagnosis of pathological conditions associated with impaired lipid metabolism, and is intended for screening and occupational examinations at work, for use in hospitals having a low level of laboratory equipment.

Diseases associated with the pathology of lipid metabolism have an increasing spread. The most common violation of lipid metabolism is the excessive accumulation of cholesterol and its complexes with lipoproteins. Therefore, of great interest is the determination of the content of b-lipoproteins in the blood serum in the diagnosis of atherosclerosis, obesity, chronic liver diseases, etc.

A known method for determining b-lipoproteins by the turbidimetric method according to Burstein and Samai, the essence of which is the ability of heparin to form a complex with serum b-lipoproteins, which precipitates under the influence of calcium chloride. The degree of turbidity of the solution is proportional to the content of b-lipoproteins in serum. Based on the values of the extinctions of the solutions measured on a photoelectrocolorimeter, the concentration of b-lipoproteins is calculated (E. Stroev, Workshop on Biological Chemistry / E.A. Stroev and V.G. Makarov. - M.: Higher School, 1986. - 126 p. .).

However, the application of this method when conducting screening programs in the first stages of a patient examination is not rational. It is longer and more expensive, since it includes the use of laboratory equipment (photoelectrocolorimeter, cuvettes, etc.).

The objective of the present invention is to develop the simplest method for studying the content of b-lipoproteins, which would allow to determine their approximate concentration during the examination of a large number of patients, when the speed of analysis is of no small importance, and would be available for medical institutions that are not adequately equipped with laboratory devices.

The problem is solved in that in the method for the rapid determination of b-lipoproteins in blood serum, including the use of calcium chloride and heparin, their effect on serum, the formation of a complex compound and turbidity of the serum, the content is visually assessed, for this a sample of text is used, typed in bold font No. 14, which is placed for reading at a distance of best vision - about 25 cm, and a test tube with the test solution is placed in front of the text, close to it, the ability to read the text and determines the content of b-lipoproteins, not exceeding 400 mg%, the lack of visibility of the text determines the concentration of b-lipoproteins above normal.

The method is as follows.

Blood from patients for research is taken in a test tube on an empty stomach. By centrifugation at 1,500 rpm or by sedimentation, the serum is separated from the suspension of red blood cells. 0.2 ml of blood serum is pipetted into a chemical test tube (tubes of the same height and diameter), then 2 ml of a 0.28% calcium chloride solution are mixed, mixed and 0.04 ml of heparin with an activity of 1000 units per ml is added, and the solution becomes turbid . After re-mixing after 4 min, an indicative concentration of b-lipoproteins is visually determined. For this purpose, use the reading of the following text developed:

The text is in boldface No. 14. A sample of text is placed for reading at a distance of best view (about 25 cm). A tube with a clouded solution is brought close to the text. If the text can be read through the solution layer, then the content of b-lipoproteins is not more than 400 mg%, if the text is viewed, but not clearly, then the content is not more than 630 mg% (upper limit of norm), if letters are not visible through the solution layer, then the concentration of b-lipoproteins is higher than normal.

Execution example.

Rapid determination of b-lipoproteins in blood serum was carried out in patients with closed traumatic brain injury and closed fracture of the left shoulder.

For this, blood serum samples were taken and the content of b-lipoproteins was determined (2 parallel determinations were performed).

The content of b-lipoproteins was assessed visually. For this, a sample of text of any content was used, which was typed in boldface No. 14. Paper with typed text was placed for reading at a distance of best vision of about 25 cm. In this case, a test tube with the test solution was placed in front of the text, close to it. If the text was read, then the content of b-lipoproteins was determined, not exceeding 400 mg%. If the text was not visible, the concentration of b-lipoproteins was determined as above normal.

The proposed method is easy to use, since its implementation does not require the use of expensive research laboratory equipment. It can be used in the laboratory of any technical equipment.

The proposed method allows you to quickly determine the approximate concentration of b-lipoproteins in a large number of subjects during their medical examination and professional examinations.

In addition, the proposed method makes it possible to assess the intensity of the pathological process.

The proposed method has social significance, since in the future it will allow to carry out the necessary organizational and therapeutic measures for the prevention of atherosclerosis, obesity, chronic liver disease, hypercholesterolemia, etc.

The proposed method is tested in the biochemistry laboratory of the Federal State Institution “RRC“ WTO ”named after Academician G.A. Ilizarov.

Claims (1)

A method for the rapid determination of b-lipoproteins in blood serum, including the use of calcium chloride and heparin, their effect on serum, the formation of a complex compound and turbidity of the serum, characterized in that the content is visually evaluated using a sample of text in boldface No. 14 , which is placed for reading at a distance of best vision - about 25 cm, and a test tube with the test solution is placed in front of the text, close to it, the ability to read the text determines the content Contents b-lipoprotein, does not exceed 400 mg%, the lack of visibility of text determines the concentration of b-lipoprotein above normal.